Probiotics improve re-epithelialization of scratches infected by Porphyromonas gingivalis through up-regulating CXCL8-CXCR1/CXCR2 axis
Anaerobe. 2021 Sep 18:102458. doi: 10.1016/j.anaerobe.2021.102458. Online ahead of print.ABSTRACTPorphyromonas gingivalis inhibits the release of CXCL8 by gingival epithelial cells and reduces their proliferation. We previously reported that Bifidocaterium sp. and Lactobacillus sp. immunomodulate gingival epithelial cells response to this periodontal pathogen, but their effects on re-epithelialization properties are still unknown. Herein we explored these activities of potential probiotics on gingival epithelial cells and clarified their mechanisms. The immortalized OBA-9 lineage was used to perform in vitro scratches. Twe...
Source: Anaerobe - September 21, 2021 Category: Microbiology Authors: Emmanuel Albuquerque-Souza Karin Hitomi Ishikawa P âmela Penas Amado Jacques Robert Nicoli Marinella Holzhausen Marcia P A Mayer Source Type: research

Probiotics improve re-epithelialization of scratches infected by Porphyromonas gingivalis through up-regulating CXCL8-CXCR1/CXCR2 axis
Anaerobe. 2021 Sep 18:102458. doi: 10.1016/j.anaerobe.2021.102458. Online ahead of print.ABSTRACTPorphyromonas gingivalis inhibits the release of CXCL8 by gingival epithelial cells and reduces their proliferation. We previously reported that Bifidocaterium sp. and Lactobacillus sp. immunomodulate gingival epithelial cells response to this periodontal pathogen, but their effects on re-epithelialization properties are still unknown. Herein we explored these activities of potential probiotics on gingival epithelial cells and clarified their mechanisms. The immortalized OBA-9 lineage was used to perform in vitro scratches. Twe...
Source: Anaerobe - September 21, 2021 Category: Microbiology Authors: Emmanuel Albuquerque-Souza Karin Hitomi Ishikawa P âmela Penas Amado Jacques Robert Nicoli Marinella Holzhausen Marcia P A Mayer Source Type: research

Label-free quantitative proteomic analysis of the oral bacteria Fusobacterium nucleatum and Porphyromonas gingivalis to identify protein features relevant in biofilm formation
CONCLUSIONS: The findings suggest that P. gingivalis reduces the production of multiple proteins because of the F. nucleatum presence. The results highlight the complex interactions of bacteria contributing to oral biofilms, which need to be considered in the design of prevention strategies.PMID:34543761 | DOI:10.1016/j.anaerobe.2021.102449 (Source: Anaerobe)
Source: Anaerobe - September 20, 2021 Category: Microbiology Authors: Marwan Mansoor Ali Mohammed Veronika Kucha řová Pettersen Audun H Nerland Harald G Wiker Vidar Bakken Source Type: research

Label-free quantitative proteomic analysis of the oral bacteria Fusobacterium nucleatum and Porphyromonas gingivalis to identify protein features relevant in biofilm formation
CONCLUSIONS: The findings suggest that P. gingivalis reduces the production of multiple proteins because of the F. nucleatum presence. The results highlight the complex interactions of bacteria contributing to oral biofilms, which need to be considered in the design of prevention strategies.PMID:34543761 | DOI:10.1016/j.anaerobe.2021.102449 (Source: Anaerobe)
Source: Anaerobe - September 20, 2021 Category: Microbiology Authors: Marwan Mansoor Ali Mohammed Veronika Kucha řová Pettersen Audun H Nerland Harald G Wiker Vidar Bakken Source Type: research

Rapid detection and surveillance of cfiA-positive Bacteroides fragilis using matrix-assisted laser desorption ionization time-of-flight mass spectrometry
CONCLUSION: The MBT Subtyping Module can detect cfiA-positive B. fragilis rapidly and accurately, supporting its use for surveillance of cfiA-positive B. fragilis in clinical settings.PMID:34537378 | DOI:10.1016/j.anaerobe.2021.102448 (Source: Anaerobe)
Source: Anaerobe - September 19, 2021 Category: Microbiology Authors: Yasuhide Kawamoto Kosuke Kosai Kenji Ota Naoki Uno Kei Sakamoto Hiroo Hasegawa Koichi Izumikawa Hiroshi Mukae Katsunori Yanagihara Source Type: research

Gardnerella vaginalis in urinary tract infections, are men spared?
Anaerobe. 2021 Sep 14;72:102438. doi: 10.1016/j.anaerobe.2021.102438. Online ahead of print.ABSTRACTGardnerella vaginalis in association with anaerobes has been linked to bacterial vaginosis in women, while urinary tract infections (UTIs) in men have rarely been reported. The aim of the review was to reveal the significance of G. vaginalis UTIs in men. Prevalence of G. vaginalis UTIs in men varied from 0.5 to>27% according to patients' groups. Most patients had comorbidity such as urolithiasis or stents, transplants, tumors and diabetes, however, infections can also affect immunocompetent patients. We observed G. vagina...
Source: Anaerobe - September 16, 2021 Category: Microbiology Authors: Lyudmila Boyanova Yulia Marteva-Proevska Raina Gergova Rumyana Markovska Source Type: research

Etiology and antimicrobial susceptibility profiles of anaerobic bacteria isolated from clinical samples in a university hospital in Madrid, Spain
CONCLUSION: Antimicrobial susceptibility surveillance of anaerobic bacteria should be performed to monitor changes in resistance patterns and to be able to optimize empiric antimicrobial treatment. Reliable species identification and quick reporting of results would guide clinicians to select the optimal antimicrobial therapy.PMID:34520862 | DOI:10.1016/j.anaerobe.2021.102446 (Source: Anaerobe)
Source: Anaerobe - September 14, 2021 Category: Microbiology Authors: J M L ópez-Pintor S Garc ía-Fernández M Ponce-Alonso A M S ánchez-Díaz P Ruiz-Garbajosa M I Morosini R Cant ón Source Type: research

Etiology and antimicrobial susceptibility profiles of anaerobic bacteria isolated from clinical samples in a university hospital in Madrid, Spain
CONCLUSION: Antimicrobial susceptibility surveillance of anaerobic bacteria should be performed to monitor changes in resistance patterns and to be able to optimize empiric antimicrobial treatment. Reliable species identification and quick reporting of results would guide clinicians to select the optimal antimicrobial therapy.PMID:34520862 | DOI:10.1016/j.anaerobe.2021.102446 (Source: Anaerobe)
Source: Anaerobe - September 14, 2021 Category: Microbiology Authors: J M L ópez-Pintor S Garc ía-Fernández M Ponce-Alonso A M S ánchez-Díaz P Ruiz-Garbajosa M I Morosini R Cant ón Source Type: research

Etiology and antimicrobial susceptibility profiles of anaerobic bacteria isolated from clinical samples in a university hospital in Madrid, Spain
CONCLUSION: Antimicrobial susceptibility surveillance of anaerobic bacteria should be performed to monitor changes in resistance patterns and to be able to optimize empiric antimicrobial treatment. Reliable species identification and quick reporting of results would guide clinicians to select the optimal antimicrobial therapy.PMID:34520862 | DOI:10.1016/j.anaerobe.2021.102446 (Source: Anaerobe)
Source: Anaerobe - September 14, 2021 Category: Microbiology Authors: J M L ópez-Pintor S Garc ía-Fernández M Ponce-Alonso A M S ánchez-Díaz P Ruiz-Garbajosa M I Morosini R Cant ón Source Type: research

Etiology and antimicrobial susceptibility profiles of anaerobic bacteria isolated from clinical samples in a university hospital in Madrid, Spain
CONCLUSION: Antimicrobial susceptibility surveillance of anaerobic bacteria should be performed to monitor changes in resistance patterns and to be able to optimize empiric antimicrobial treatment. Reliable species identification and quick reporting of results would guide clinicians to select the optimal antimicrobial therapy.PMID:34520862 | DOI:10.1016/j.anaerobe.2021.102446 (Source: Anaerobe)
Source: Anaerobe - September 14, 2021 Category: Microbiology Authors: J M L ópez-Pintor S Garc ía-Fernández M Ponce-Alonso A M S ánchez-Díaz P Ruiz-Garbajosa M I Morosini R Cant ón Source Type: research

Etiology and antimicrobial susceptibility profiles of anaerobic bacteria isolated from clinical samples in a university hospital in Madrid, Spain
CONCLUSION: Antimicrobial susceptibility surveillance of anaerobic bacteria should be performed to monitor changes in resistance patterns and to be able to optimize empiric antimicrobial treatment. Reliable species identification and quick reporting of results would guide clinicians to select the optimal antimicrobial therapy.PMID:34520862 | DOI:10.1016/j.anaerobe.2021.102446 (Source: Anaerobe)
Source: Anaerobe - September 14, 2021 Category: Microbiology Authors: J M L ópez-Pintor S Garc ía-Fernández M Ponce-Alonso A M S ánchez-Díaz P Ruiz-Garbajosa M I Morosini R Cant ón Source Type: research

Non-steroidal anti-inflammatory drugs are not associated with increased risk of Clostridioides difficile infection: A propensity-score-matched case-control study
Anaerobe. 2021 Sep 7:102444. doi: 10.1016/j.anaerobe.2021.102444. Online ahead of print.ABSTRACTPrior research identified an increased risk for Clostridioides difficile infection (CDI) following exposure to certain non-steroidal anti-inflammatory drugs (NSAIDs). We conducted a retrospective case-control study to evaluate the risk for CDI associated with NSAID use. NSAID use was not associated with an increased risk of CDI.PMID:34506930 | DOI:10.1016/j.anaerobe.2021.102444 (Source: Anaerobe)
Source: Anaerobe - September 10, 2021 Category: Microbiology Authors: Adam M Ressler Alieysa Patel Krishna Rao Source Type: research

Non-steroidal anti-inflammatory drugs are not associated with increased risk of Clostridioides difficile infection: A propensity-score-matched case-control study
Anaerobe. 2021 Sep 7:102444. doi: 10.1016/j.anaerobe.2021.102444. Online ahead of print.ABSTRACTPrior research identified an increased risk for Clostridioides difficile infection (CDI) following exposure to certain non-steroidal anti-inflammatory drugs (NSAIDs). We conducted a retrospective case-control study to evaluate the risk for CDI associated with NSAID use. NSAID use was not associated with an increased risk of CDI.PMID:34506930 | DOI:10.1016/j.anaerobe.2021.102444 (Source: Anaerobe)
Source: Anaerobe - September 10, 2021 Category: Microbiology Authors: Adam M Ressler Alieysa Patel Krishna Rao Source Type: research

Non-steroidal anti-inflammatory drugs are not associated with increased risk of Clostridioides difficile infection: A propensity-score-matched case-control study
Anaerobe. 2021 Sep 7:102444. doi: 10.1016/j.anaerobe.2021.102444. Online ahead of print.ABSTRACTPrior research identified an increased risk for Clostridioides difficile infection (CDI) following exposure to certain non-steroidal anti-inflammatory drugs (NSAIDs). We conducted a retrospective case-control study to evaluate the risk for CDI associated with NSAID use. NSAID use was not associated with an increased risk of CDI.PMID:34506930 | DOI:10.1016/j.anaerobe.2021.102444 (Source: Anaerobe)
Source: Anaerobe - September 10, 2021 Category: Microbiology Authors: Adam M Ressler Alieysa Patel Krishna Rao Source Type: research

Comparative evaluation of agar dilution and broth microdilution by commercial and in-house plates for Bacteroides fragilis group: An economical and expeditious approach for resource-limited settings
CONCLUSION: BMD gives comparable results to agar dilution and can be considered a method of choice to test a small number of samples. The technique is an economical option when plates are standardized in-house and could be employed for susceptibility testing of the B. fragilis group.PMID:34492368 | DOI:10.1016/j.anaerobe.2021.102443 (Source: Anaerobe)
Source: Anaerobe - September 7, 2021 Category: Microbiology Authors: Anshul Sood Archana Angrup Pallab Ray Kiran Bala Source Type: research

Comparative evaluation of agar dilution and broth microdilution by commercial and in-house plates for Bacteroides fragilis group: An economical and expeditious approach for resource-limited settings
CONCLUSION: BMD gives comparable results to agar dilution and can be considered a method of choice to test a small number of samples. The technique is an economical option when plates are standardized in-house and could be employed for susceptibility testing of the B. fragilis group.PMID:34492368 | DOI:10.1016/j.anaerobe.2021.102443 (Source: Anaerobe)
Source: Anaerobe - September 7, 2021 Category: Microbiology Authors: Anshul Sood Archana Angrup Pallab Ray Kiran Bala Source Type: research

Comparative evaluation of agar dilution and broth microdilution by commercial and in-house plates for Bacteroides fragilis group: An economical and expeditious approach for resource-limited settings
CONCLUSION: BMD gives comparable results to agar dilution and can be considered a method of choice to test a small number of samples. The technique is an economical option when plates are standardized in-house and could be employed for susceptibility testing of the B. fragilis group.PMID:34492368 | DOI:10.1016/j.anaerobe.2021.102443 (Source: Anaerobe)
Source: Anaerobe - September 7, 2021 Category: Microbiology Authors: Anshul Sood Archana Angrup Pallab Ray Kiran Bala Source Type: research

Comparative evaluation of agar dilution and broth microdilution by commercial and in-house plates for Bacteroides fragilis group: An economical and expeditious approach for resource-limited settings
CONCLUSION: BMD gives comparable results to agar dilution and can be considered a method of choice to test a small number of samples. The technique is an economical option when plates are standardized in-house and could be employed for susceptibility testing of the B. fragilis group.PMID:34492368 | DOI:10.1016/j.anaerobe.2021.102443 (Source: Anaerobe)
Source: Anaerobe - September 7, 2021 Category: Microbiology Authors: Anshul Sood Archana Angrup Pallab Ray Kiran Bala Source Type: research

A rare cause of bacteremia due to Porphyromonas asaccharolytica in a patient with necrotizing fasciitis
We report an uncommon case of bacteremia due to P. asaccharolytica in a patient with necrotizing fasciitis. A 52-year-old woman with a history of diabetes mellitus was admitted for an extensive necrotizing lesion on the left lower limb. After she developed septic shock, two sets of blood cultures were taken. Anaerobic bottles yielded a pure culture of a microorganism initially identified as P. uenonis by MALDI-TOF MS but with a low log score, and a gene sequencing technique was therefore applied, identifying the isolate as P. asaccharolytica. Only resistance to penicillin and clindamycin was documented. Treatment with mero...
Source: Anaerobe - September 5, 2021 Category: Microbiology Authors: Fernando Cobo Virginia P érez-Carrasco Victoria S ánchez-Martin Jos é A García-Salcedo Elvira Barr ón Martín Jos é María Navarro-Marí Source Type: research

Comparative clinical outcomes evaluation of hospitalized patients infected with Clostridioides difficile ribotype 106 vs. other toxigenic strains
Anaerobe. 2021 Aug 27:102440. doi: 10.1016/j.anaerobe.2021.102440. Online ahead of print.ABSTRACTBACKGROUND: Although Clostridioides difficile surveillance often identifies emerging strains, clinical outcome evaluations are rarely performed. Ribotype (RT) 106 is a commonly isolated C. difficile strain worldwide; however, studies investigating RT 106 clinical outcomes are limited. The purpose of this study was to investigate clinical outcomes of RT 106 infections compared with two other endemic strains of varying virulence.METHODS: This multicenter study evaluated adults hospitalized with C. difficile infection (CDI). C. di...
Source: Anaerobe - August 30, 2021 Category: Microbiology Authors: Masaad Saeed Almutairi Anne J Gonzales-Luna Faris S Alnezary Saad Fallatah M Jahangir Alam Khurshida Begum Kevin W Garey Source Type: research

Add-on interventions for the prevention of recurrent Clostridioides Difficile infection: A systematic review and network meta-analysis
CONCLUSION: Add-on treatment with oligofructose, NTCD-M3 spores, rifaximin, RBX2660, and bezlotoxumab likely reduces the risk of CDI. Evidence on probiotics and SER-109 are uncertain, thus adequately powered trials are warranted.PMID:34454094 | DOI:10.1016/j.anaerobe.2021.102441 (Source: Anaerobe)
Source: Anaerobe - August 28, 2021 Category: Microbiology Authors: Paschalis Paschos Konstantinos Ioakim Konstantinos Malandris Argyro Koukoufiki Tarek Nayfeh Evangelos Akriviadis Apostolos Tsapas Eleni Bekiari Source Type: research

Targeting antibiotic tolerance in anaerobic biofilms associated with oral diseases: Human antimicrobial peptides LL-37 and lactoferricin enhance the antibiotic efficacy of amoxicillin, clindamycin and metronidazole
In this study, we examined the effects of two endogenous human antimicrobial peptides, LL-37 and human Lactoferricin, on the antibiotic drug efficacy of amoxicillin, clindamycin and metronidazole in two types of polymicrobial biofilms, which aimed to represent frequent oral diseases: (1) facultative anaerobic (Streptococcus mutans, Streptococcus sanguinis, Actinomyces naeslundii) and (2) obligate anaerobic biofilms (Veillonella parvula, Parvimonas micra, Fusobacterium nucleatum). LL-37 and Lactoferricin enhanced the anti-biofilm effect of amoxicillin and clindamycin in facultative anaerobic biofilms. Metronidazole alone wa...
Source: Anaerobe - August 28, 2021 Category: Microbiology Authors: Sabina Noreen Wuersching Karin Christine Huth Reinhard Hickel Maximilian Kollmuss Source Type: research

Add-on interventions for the prevention of recurrent Clostridioides Difficile infection: A systematic review and network meta-analysis
CONCLUSION: Add-on treatment with oligofructose, NTCD-M3 spores, rifaximin, RBX2660, and bezlotoxumab likely reduces the risk of CDI. Evidence on probiotics and SER-109 are uncertain, thus adequately powered trials are warranted.PMID:34454094 | DOI:10.1016/j.anaerobe.2021.102441 (Source: Anaerobe)
Source: Anaerobe - August 28, 2021 Category: Microbiology Authors: Paschalis Paschos Konstantinos Ioakim Konstantinos Malandris Argyro Koukoufiki Tarek Nayfeh Evangelos Akriviadis Apostolos Tsapas Eleni Bekiari Source Type: research

Targeting antibiotic tolerance in anaerobic biofilms associated with oral diseases: Human antimicrobial peptides LL-37 and lactoferricin enhance the antibiotic efficacy of amoxicillin, clindamycin and metronidazole
In this study, we examined the effects of two endogenous human antimicrobial peptides, LL-37 and human Lactoferricin, on the antibiotic drug efficacy of amoxicillin, clindamycin and metronidazole in two types of polymicrobial biofilms, which aimed to represent frequent oral diseases: (1) facultative anaerobic (Streptococcus mutans, Streptococcus sanguinis, Actinomyces naeslundii) and (2) obligate anaerobic biofilms (Veillonella parvula, Parvimonas micra, Fusobacterium nucleatum). LL-37 and Lactoferricin enhanced the anti-biofilm effect of amoxicillin and clindamycin in facultative anaerobic biofilms. Metronidazole alone wa...
Source: Anaerobe - August 28, 2021 Category: Microbiology Authors: Sabina Noreen Wuersching Karin Christine Huth Reinhard Hickel Maximilian Kollmuss Source Type: research

Add-on interventions for the prevention of recurrent Clostridioides Difficile infection: A systematic review and network meta-analysis
CONCLUSION: Add-on treatment with oligofructose, NTCD-M3 spores, rifaximin, RBX2660, and bezlotoxumab likely reduces the risk of CDI. Evidence on probiotics and SER-109 are uncertain, thus adequately powered trials are warranted.PMID:34454094 | DOI:10.1016/j.anaerobe.2021.102441 (Source: Anaerobe)
Source: Anaerobe - August 28, 2021 Category: Microbiology Authors: Paschalis Paschos Konstantinos Ioakim Konstantinos Malandris Argyro Koukoufiki Tarek Nayfeh Evangelos Akriviadis Apostolos Tsapas Eleni Bekiari Source Type: research

Targeting antibiotic tolerance in anaerobic biofilms associated with oral diseases: Human antimicrobial peptides LL-37 and lactoferricin enhance the antibiotic efficacy of amoxicillin, clindamycin and metronidazole
In this study, we examined the effects of two endogenous human antimicrobial peptides, LL-37 and human Lactoferricin, on the antibiotic drug efficacy of amoxicillin, clindamycin and metronidazole in two types of polymicrobial biofilms, which aimed to represent frequent oral diseases: (1) facultative anaerobic (Streptococcus mutans, Streptococcus sanguinis, Actinomyces naeslundii) and (2) obligate anaerobic biofilms (Veillonella parvula, Parvimonas micra, Fusobacterium nucleatum). LL-37 and Lactoferricin enhanced the anti-biofilm effect of amoxicillin and clindamycin in facultative anaerobic biofilms. Metronidazole alone wa...
Source: Anaerobe - August 28, 2021 Category: Microbiology Authors: Sabina Noreen Wuersching Karin Christine Huth Reinhard Hickel Maximilian Kollmuss Source Type: research

What's a SNP between friends: The lineage of Clostridioides difficile R20291 can effect research outcomes
Anaerobe. 2021 Jul 31;71:102422. doi: 10.1016/j.anaerobe.2021.102422. Online ahead of print.ABSTRACTClostridioides difficile R20291 is the most studied PCR-Ribotype 027 isolate. The two predominant lineages of this hypervirulent strain, however, exhibit substantive phenotypic differences and possess genomes that differ by a small number of nucleotide changes. It is important that the source of R20291 is taken into account in research outcomes.PMID:34343672 | DOI:10.1016/j.anaerobe.2021.102422 (Source: Anaerobe)
Source: Anaerobe - August 3, 2021 Category: Microbiology Authors: Jorge Monteford Terry W Bilverstone Patrick Ingle Sheryl Philip Sarah A Kuehne Nigel P Minton Source Type: research

What's a SNP between friends: The lineage of Clostridioides difficile R20291 can effect research outcomes
Anaerobe. 2021 Jul 31;71:102422. doi: 10.1016/j.anaerobe.2021.102422. Online ahead of print.ABSTRACTClostridioides difficile R20291 is the most studied PCR-Ribotype 027 isolate. The two predominant lineages of this hypervirulent strain, however, exhibit substantive phenotypic differences and possess genomes that differ by a small number of nucleotide changes. It is important that the source of R20291 is taken into account in research outcomes.PMID:34343672 | DOI:10.1016/j.anaerobe.2021.102422 (Source: Anaerobe)
Source: Anaerobe - August 3, 2021 Category: Microbiology Authors: Jorge Monteford Terry W Bilverstone Patrick Ingle Sheryl Philip Sarah A Kuehne Nigel P Minton Source Type: research

What's a SNP between friends: The lineage of Clostridioides difficile R20291 can effect research outcomes
Anaerobe. 2021 Jul 31;71:102422. doi: 10.1016/j.anaerobe.2021.102422. Online ahead of print.ABSTRACTClostridioides difficile R20291 is the most studied PCR-Ribotype 027 isolate. The two predominant lineages of this hypervirulent strain, however, exhibit substantive phenotypic differences and possess genomes that differ by a small number of nucleotide changes. It is important that the source of R20291 is taken into account in research outcomes.PMID:34343672 | DOI:10.1016/j.anaerobe.2021.102422 (Source: Anaerobe)
Source: Anaerobe - August 3, 2021 Category: Microbiology Authors: Jorge Monteford Terry W Bilverstone Patrick Ingle Sheryl Philip Sarah A Kuehne Nigel P Minton Source Type: research

What's a SNP between friends: The lineage of Clostridioides difficile R20291 can effect research outcomes
Anaerobe. 2021 Jul 31;71:102422. doi: 10.1016/j.anaerobe.2021.102422. Online ahead of print.ABSTRACTClostridioides difficile R20291 is the most studied PCR-Ribotype 027 isolate. The two predominant lineages of this hypervirulent strain, however, exhibit substantive phenotypic differences and possess genomes that differ by a small number of nucleotide changes. It is important that the source of R20291 is taken into account in research outcomes.PMID:34343672 | DOI:10.1016/j.anaerobe.2021.102422 (Source: Anaerobe)
Source: Anaerobe - August 3, 2021 Category: Microbiology Authors: Jorge Monteford Terry W Bilverstone Patrick Ingle Sheryl Philip Sarah A Kuehne Nigel P Minton Source Type: research

What's a SNP between friends: The lineage of Clostridioides difficile R20291 can effect research outcomes
Anaerobe. 2021 Jul 31;71:102422. doi: 10.1016/j.anaerobe.2021.102422. Online ahead of print.ABSTRACTClostridioides difficile R20291 is the most studied PCR-Ribotype 027 isolate. The two predominant lineages of this hypervirulent strain, however, exhibit substantive phenotypic differences and possess genomes that differ by a small number of nucleotide changes. It is important that the source of R20291 is taken into account in research outcomes.PMID:34343672 | DOI:10.1016/j.anaerobe.2021.102422 (Source: Anaerobe)
Source: Anaerobe - August 3, 2021 Category: Microbiology Authors: Jorge Monteford Terry W Bilverstone Patrick Ingle Sheryl Philip Sarah A Kuehne Nigel P Minton Source Type: research

What's a SNP between friends: The lineage of Clostridioides difficile R20291 can effect research outcomes
Anaerobe. 2021 Jul 31;71:102422. doi: 10.1016/j.anaerobe.2021.102422. Online ahead of print.ABSTRACTClostridioides difficile R20291 is the most studied PCR-Ribotype 027 isolate. The two predominant lineages of this hypervirulent strain, however, exhibit substantive phenotypic differences and possess genomes that differ by a small number of nucleotide changes. It is important that the source of R20291 is taken into account in research outcomes.PMID:34343672 | DOI:10.1016/j.anaerobe.2021.102422 (Source: Anaerobe)
Source: Anaerobe - August 3, 2021 Category: Microbiology Authors: Jorge Monteford Terry W Bilverstone Patrick Ingle Sheryl Philip Sarah A Kuehne Nigel P Minton Source Type: research

What's a SNP between friends: The lineage of Clostridioides difficile R20291 can effect research outcomes
Anaerobe. 2021 Jul 31;71:102422. doi: 10.1016/j.anaerobe.2021.102422. Online ahead of print.ABSTRACTClostridioides difficile R20291 is the most studied PCR-Ribotype 027 isolate. The two predominant lineages of this hypervirulent strain, however, exhibit substantive phenotypic differences and possess genomes that differ by a small number of nucleotide changes. It is important that the source of R20291 is taken into account in research outcomes.PMID:34343672 | DOI:10.1016/j.anaerobe.2021.102422 (Source: Anaerobe)
Source: Anaerobe - August 3, 2021 Category: Microbiology Authors: Jorge Monteford Terry W Bilverstone Patrick Ingle Sheryl Philip Sarah A Kuehne Nigel P Minton Source Type: research

What's a SNP between friends: The lineage of Clostridioides difficile R20291 can effect research outcomes
Anaerobe. 2021 Jul 31;71:102422. doi: 10.1016/j.anaerobe.2021.102422. Online ahead of print.ABSTRACTClostridioides difficile R20291 is the most studied PCR-Ribotype 027 isolate. The two predominant lineages of this hypervirulent strain, however, exhibit substantive phenotypic differences and possess genomes that differ by a small number of nucleotide changes. It is important that the source of R20291 is taken into account in research outcomes.PMID:34343672 | DOI:10.1016/j.anaerobe.2021.102422 (Source: Anaerobe)
Source: Anaerobe - August 3, 2021 Category: Microbiology Authors: Jorge Monteford Terry W Bilverstone Patrick Ingle Sheryl Philip Sarah A Kuehne Nigel P Minton Source Type: research

What's a SNP between friends: The lineage of Clostridioides difficile R20291 can effect research outcomes
Anaerobe. 2021 Jul 31;71:102422. doi: 10.1016/j.anaerobe.2021.102422. Online ahead of print.ABSTRACTClostridioides difficile R20291 is the most studied PCR-Ribotype 027 isolate. The two predominant lineages of this hypervirulent strain, however, exhibit substantive phenotypic differences and possess genomes that differ by a small number of nucleotide changes. It is important that the source of R20291 is taken into account in research outcomes.PMID:34343672 | DOI:10.1016/j.anaerobe.2021.102422 (Source: Anaerobe)
Source: Anaerobe - August 3, 2021 Category: Microbiology Authors: Jorge Monteford Terry W Bilverstone Patrick Ingle Sheryl Philip Sarah A Kuehne Nigel P Minton Source Type: research

What's a SNP between friends: The lineage of Clostridioides difficile R20291 can effect research outcomes
Anaerobe. 2021 Jul 31;71:102422. doi: 10.1016/j.anaerobe.2021.102422. Online ahead of print.ABSTRACTClostridioides difficile R20291 is the most studied PCR-Ribotype 027 isolate. The two predominant lineages of this hypervirulent strain, however, exhibit substantive phenotypic differences and possess genomes that differ by a small number of nucleotide changes. It is important that the source of R20291 is taken into account in research outcomes.PMID:34343672 | DOI:10.1016/j.anaerobe.2021.102422 (Source: Anaerobe)
Source: Anaerobe - August 3, 2021 Category: Microbiology Authors: Jorge Monteford Terry W Bilverstone Patrick Ingle Sheryl Philip Sarah A Kuehne Nigel P Minton Source Type: research

What's a SNP between friends: The lineage of Clostridioides difficile R20291 can effect research outcomes
Anaerobe. 2021 Jul 31;71:102422. doi: 10.1016/j.anaerobe.2021.102422. Online ahead of print.ABSTRACTClostridioides difficile R20291 is the most studied PCR-Ribotype 027 isolate. The two predominant lineages of this hypervirulent strain, however, exhibit substantive phenotypic differences and possess genomes that differ by a small number of nucleotide changes. It is important that the source of R20291 is taken into account in research outcomes.PMID:34343672 | DOI:10.1016/j.anaerobe.2021.102422 (Source: Anaerobe)
Source: Anaerobe - August 3, 2021 Category: Microbiology Authors: Jorge Monteford Terry W Bilverstone Patrick Ingle Sheryl Philip Sarah A Kuehne Nigel P Minton Source Type: research

What's a SNP between friends: The lineage of Clostridioides difficile R20291 can effect research outcomes
Anaerobe. 2021 Jul 31:102422. doi: 10.1016/j.anaerobe.2021.102422. Online ahead of print.ABSTRACTClostridioides difficile R20291 is the most studied PCR-Ribotype 027 isolate. The two predominant lineages of this hypervirulent strain, however, exhibit substantive phenotypic differences and possess genomes that differ by a small number of nucleotide changes. It is important that the source of R20291 is taken into account in research outcomes.PMID:34343672 | DOI:10.1016/j.anaerobe.2021.102422 (Source: Anaerobe)
Source: Anaerobe - August 3, 2021 Category: Microbiology Authors: Jorge Monteford Terry W Bilverstone Patrick Ingle Sheryl Philip Sarah A Kuehne Nigel P Minton Source Type: research

What's a SNP between friends: The lineage of Clostridioides difficile R20291 can effect research outcomes
Anaerobe. 2021 Jul 31:102422. doi: 10.1016/j.anaerobe.2021.102422. Online ahead of print.ABSTRACTClostridioides difficile R20291 is the most studied PCR-Ribotype 027 isolate. The two predominant lineages of this hypervirulent strain, however, exhibit substantive phenotypic differences and possess genomes that differ by a small number of nucleotide changes. It is important that the source of R20291 is taken into account in research outcomes.PMID:34343672 | DOI:10.1016/j.anaerobe.2021.102422 (Source: Anaerobe)
Source: Anaerobe - August 3, 2021 Category: Microbiology Authors: Jorge Monteford Terry W Bilverstone Patrick Ingle Sheryl Philip Sarah A Kuehne Nigel P Minton Source Type: research

What's a SNP between friends: The lineage of Clostridioides difficile R20291 can effect research outcomes
Anaerobe. 2021 Jul 31:102422. doi: 10.1016/j.anaerobe.2021.102422. Online ahead of print.ABSTRACTClostridioides difficile R20291 is the most studied PCR-Ribotype 027 isolate. The two predominant lineages of this hypervirulent strain, however, exhibit substantive phenotypic differences and possess genomes that differ by a small number of nucleotide changes. It is important that the source of R20291 is taken into account in research outcomes.PMID:34343672 | DOI:10.1016/j.anaerobe.2021.102422 (Source: Anaerobe)
Source: Anaerobe - August 3, 2021 Category: Microbiology Authors: Jorge Monteford Terry W Bilverstone Patrick Ingle Sheryl Philip Sarah A Kuehne Nigel P Minton Source Type: research

What's a SNP between friends: The lineage of Clostridioides difficile R20291 can effect research outcomes
Anaerobe. 2021 Jul 31:102422. doi: 10.1016/j.anaerobe.2021.102422. Online ahead of print.ABSTRACTClostridioides difficile R20291 is the most studied PCR-Ribotype 027 isolate. The two predominant lineages of this hypervirulent strain, however, exhibit substantive phenotypic differences and possess genomes that differ by a small number of nucleotide changes. It is important that the source of R20291 is taken into account in research outcomes.PMID:34343672 | DOI:10.1016/j.anaerobe.2021.102422 (Source: Anaerobe)
Source: Anaerobe - August 3, 2021 Category: Microbiology Authors: Jorge Monteford Terry W Bilverstone Patrick Ingle Sheryl Philip Sarah A Kuehne Nigel P Minton Source Type: research

Clostridium innocuum: Microbiological and clinical characteristics of a potential emerging pathogen
Anaerobe. 2021 Jul 28:102418. doi: 10.1016/j.anaerobe.2021.102418. Online ahead of print.ABSTRACTClostridium innocuum is an anaerobic, gram-positive, spore-forming bacterium identified by Smith and King in 1962 after being isolated from a patient with an appendiceal abscess. Its name, C. innocuum, reflected its clinically "innocuous" nature based on observed lack of virulence in animal models of infection. Since that time, C. innocuum has been identified as both part of the normal intestinal flora and the cause of a rare, intrinsically vancomycin-resistant opportunistic infection in immunocompromised patients. Mo...
Source: Anaerobe - July 31, 2021 Category: Microbiology Authors: Kathryn E Cherny Emily B Muscat Megan E Reyna Larry K Kociolek Source Type: research

Clostridium innocuum: Microbiological and clinical characteristics of a potential emerging pathogen
Anaerobe. 2021 Jul 28:102418. doi: 10.1016/j.anaerobe.2021.102418. Online ahead of print.ABSTRACTClostridium innocuum is an anaerobic, gram-positive, spore-forming bacterium identified by Smith and King in 1962 after being isolated from a patient with an appendiceal abscess. Its name, C. innocuum, reflected its clinically "innocuous" nature based on observed lack of virulence in animal models of infection. Since that time, C. innocuum has been identified as both part of the normal intestinal flora and the cause of a rare, intrinsically vancomycin-resistant opportunistic infection in immunocompromised patients. Mo...
Source: Anaerobe - July 31, 2021 Category: Microbiology Authors: Kathryn E Cherny Emily B Muscat Megan E Reyna Larry K Kociolek Source Type: research

Clostridium innocuum: Microbiological and clinical characteristics of a potential emerging pathogen
Anaerobe. 2021 Jul 28:102418. doi: 10.1016/j.anaerobe.2021.102418. Online ahead of print.ABSTRACTClostridium innocuum is an anaerobic, gram-positive, spore-forming bacterium identified by Smith and King in 1962 after being isolated from a patient with an appendiceal abscess. Its name, C. innocuum, reflected its clinically "innocuous" nature based on observed lack of virulence in animal models of infection. Since that time, C. innocuum has been identified as both part of the normal intestinal flora and the cause of a rare, intrinsically vancomycin-resistant opportunistic infection in immunocompromised patients. Mo...
Source: Anaerobe - July 31, 2021 Category: Microbiology Authors: Kathryn E Cherny Emily B Muscat Megan E Reyna Larry K Kociolek Source Type: research

Septic hip abscess due to Fusobacterium nucleatum and Actinomyces turicensis in an immunocompetent SARS-CoV-2 positive patient
Anaerobe. 2021 Jul 24;71:102420. doi: 10.1016/j.anaerobe.2021.102420. Online ahead of print.ABSTRACTA 42-year-old man was referred to the Department of Orthopedic Surgery with pain over his right greater trochanter and signs of systemic infection. CT showed an enhanced mass in his gluteus maximus as well as gas in the biceps femoris over the underlying hip joint. Tissue biopsy yielded Fusobacterium nucleatum and Actinomyces turicensis. The patient was successfully treated for 6 weeks with amoxicillin/clavulanic acid 875mg/125mg and metronidazole 500mg.PMID:34314865 | PMC:PMC8305297 | DOI:10.1016/j.anaerobe.2021.102420 (Source: Anaerobe)
Source: Anaerobe - July 27, 2021 Category: Microbiology Authors: A Nia A Ungersboeck M Uffmann D Leaper O Assadian Source Type: research

EUCAST invite comments on new methods for antimicrobial susceptibility testing of anaerobic bacteria
Anaerobe. 2021 Jul 24:102417. doi: 10.1016/j.anaerobe.2021.102417. Online ahead of print.NO ABSTRACTPMID:34314866 | DOI:10.1016/j.anaerobe.2021.102417 (Source: Anaerobe)
Source: Anaerobe - July 27, 2021 Category: Microbiology Authors: Gunnar Kahlmeter Trefor E Morris Source Type: research

Catabacter hongkongensis bacteremia identified by direct metagenomic sequencing of positive blood culture fluid, first case report in the US
Anaerobe. 2021 Jul 24:102421. doi: 10.1016/j.anaerobe.2021.102421. Online ahead of print.ABSTRACTCatabacter hongkongensis, an increasingly recognized bacteria in clinical samples, was identified by direct metagenomic sequencing of positive blood culture fluid from a 55-year-old patient with colonic perforation. The bacteremia was cleared by both antibiotic treatment and surgical intervention. This is the first case report of C. hongkongensis infection in the US.PMID:34314867 | DOI:10.1016/j.anaerobe.2021.102421 (Source: Anaerobe)
Source: Anaerobe - July 27, 2021 Category: Microbiology Authors: Edwin Kamau Enedina Maliksi Nancy Kwan Omai B Garner Shangxin Yang Source Type: research

Septic hip abscess due to Fusobacterium nucleatum and Actinomyces turicensis in an immunocompetent SARS-CoV-2 positive patient
Anaerobe. 2021 Jul 24;71:102420. doi: 10.1016/j.anaerobe.2021.102420. Online ahead of print.ABSTRACTA 42-year-old man was referred to the Department of Orthopedic Surgery with pain over his right greater trochanter and signs of systemic infection. CT showed an enhanced mass in his gluteus maximus as well as gas in the biceps femoris over the underlying hip joint. Tissue biopsy yielded Fusobacterium nucleatum and Actinomyces turicensis. The patient was successfully treated for 6 weeks with amoxicillin/clavulanic acid 875mg/125mg and metronidazole 500mg.PMID:34314865 | PMC:PMC8305297 | DOI:10.1016/j.anaerobe.2021.102420 (Source: Anaerobe)
Source: Anaerobe - July 27, 2021 Category: Microbiology Authors: A Nia A Ungersboeck M Uffmann D Leaper O Assadian Source Type: research

EUCAST invite comments on new methods for antimicrobial susceptibility testing of anaerobic bacteria
Anaerobe. 2021 Jul 24:102417. doi: 10.1016/j.anaerobe.2021.102417. Online ahead of print.NO ABSTRACTPMID:34314866 | DOI:10.1016/j.anaerobe.2021.102417 (Source: Anaerobe)
Source: Anaerobe - July 27, 2021 Category: Microbiology Authors: Gunnar Kahlmeter Trefor E Morris Source Type: research

Catabacter hongkongensis bacteremia identified by direct metagenomic sequencing of positive blood culture fluid, first case report in the US
Anaerobe. 2021 Jul 24:102421. doi: 10.1016/j.anaerobe.2021.102421. Online ahead of print.ABSTRACTCatabacter hongkongensis, an increasingly recognized bacteria in clinical samples, was identified by direct metagenomic sequencing of positive blood culture fluid from a 55-year-old patient with colonic perforation. The bacteremia was cleared by both antibiotic treatment and surgical intervention. This is the first case report of C. hongkongensis infection in the US.PMID:34314867 | DOI:10.1016/j.anaerobe.2021.102421 (Source: Anaerobe)
Source: Anaerobe - July 27, 2021 Category: Microbiology Authors: Edwin Kamau Enedina Maliksi Nancy Kwan Omai B Garner Shangxin Yang Source Type: research