Subgingival microbiota in a population with and without cognitive dysfunction.
Conclusion: It is concluded that in individuals with cognitive impairment or AD, the subgingival microbiota exhibits shifts typical of periodontal disease. PMID: 33537116 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - February 6, 2021 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Human cytomegalovirus is present in the odontogenic epithelium of ameloblastoma.
Conclusions: The high prevalence of HCMV inside the odontogenic epithelium of AB could indicate a possible role of the virus in the oncogenesis and/or oncomodulation of the AB. Additionally, we recommend the IHC for the detection of HCMV in the odontogenic tumors like AB. PMID: 33537117 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - February 6, 2021 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Enterococcus faecalis shifts macrophage polarization toward M1-like phenotype with an altered cytokine profile.
Conclusions: These findings suggested that E. faecalis infection of bone marrow-derived stem cells during differentiation into macrophages induces an atypical M1-like phenotype associated with intracellular bacterial survival. PMID: 33488991 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - January 27, 2021 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Recombinant bacteriophage T4 Rnl1 impacts Streptococcus mutans biofilm formation.
Authors: Chen J, Chen Z, Yuan K, Huang Z, Mao M Abstract Bacteriophage T4 RNA ligase 1 (T4 Rnl1) can be stably expressed in many bacteria and has been reported to affect the bioactivity of the host bacteria. Recently, we constructed bacteriophage T4 Rnl1 expressing system in Streptococcus mutans, a crucial biofilm-forming and dental caries-causing oral pathogen. Here, we characterized the function of recombinant bacteriophage T4 Rnl1 in biofilm formation of S. mutans. The T4 Rnl1 mutant exhibited similar growth phenotype but resulted in a significant reduction of biofilm biomass compared to wild type stra...
Source: Journal of Oral Microbiology - January 20, 2021 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Oral microbiome in down syndrome and its implications on oral health.
Conclusion: Altogether, our study provides a first global snapshot of the oral microbiome in DS. Future studies are required to establish whether the observed differences are related to differential pathology in the oral cavity in DS. PMID: 33456723 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - January 20, 2021 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Tetracycline and multidrug resistance in the oral microbiota: differences between healthy subjects and patients with periodontitis in Spain.
Conclusion: The high prevalence of multidrug resistance and tetracycline resistance genes found in the subgingival microbiota, highlights the importance of performing wider and more in-depth analysis of antibiotic resistance in the oral microbiota. PMID: 33391624 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - January 6, 2021 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Periodontal tissues are targets for Sars-Cov-2: a post-mortem study.
Conclusion: We presented a biomolecular analysis obtained from minimally invasive autopsies. This is the first study to demonstrate the presence of SARS-CoV-2 in periodontal tissue in COVID-19 positive patients. PMID: 33391625 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - January 6, 2021 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Fusobacteria modulate oral carcinogenesis and promote cancer progression.
Conclusion: This study demonstrated that Fusobacteria could potentially enhance cancer cell invasiveness, survival, and EMT when presented in the oral tumour microenvironment. Abbreviations: 4NQO, 4-nitroquinoline-1-oxide; ELISA, enzyme-linked immunosorbent assay; EMT, epithelial-mesenchymal transition; IL-8, interleukin-8; JAK1, Janus kinase 1; LPS, lipopolysaccharide; MMP, matrix metalloproteinase; OSCCs, oral squamous cell carcinomas; PK, proteinase K; PMB, Polymyxin B; qRT-PCR, quantitative real-time polymerase chain reaction; STAT3, signal transducer and activator of transcription 3; TGF-β, transforming growth fa...
Source: Journal of Oral Microbiology - January 6, 2021 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Porphyromonas, Treponema, and Mogibacterium promote IL8/IFN γ/TNFα-based pro-inflammation in patients with medication-related osteonecrosis of the jaw.
Porphyromonas, Treponema, and Mogibacterium promote IL8/IFNγ/TNFα-based pro-inflammation in patients with medication-related osteonecrosis of the jaw. J Oral Microbiol. 2020 Nov 23;13(1):1851112 Authors: Li Q, Pu Y, Lu H, Zhao N, Wang Y, Guo Y, Guo C Abstract Objective: Refractory infection is an important factor affecting the progression of medication-related osteonecrosis of the jaw (MRONJ) from clinical stage I to stage II/III. The aim of this study was to explore the distribution of bacteria and their association with the inflammatory pathway of stage II/III MRONJ. Materials and Methods...
Source: Journal of Oral Microbiology - January 6, 2021 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Neutrophils exhibit an individual response to different oral bacterial biofilms.
Authors: Mikolai C, Branitzki-Heinemann K, Ingendoh-Tsakmakidis A, Stiesch M, von Köckritz-Blickwede M, Winkel A Abstract Oral innate immunity is led by neutrophils. It is still unclear how their main antimicrobial mechanisms against different biofilms may contribute to balance or dysregulation in the oral cavity. We investigated the capacity of commensal (Streptococcus oralis) and pathogenic (Porphyromonas gingivalis or Aggregatibacter actinomycetemcomitans) monospecies biofilms to induce or to inhibit selected antimicrobial mechanisms of neutrophils. S. oralis induced neutrophil extracellular traps (NETs) fo...
Source: Journal of Oral Microbiology - January 6, 2021 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Salivary bacterial shifts in oral leukoplakia resemble the dysbiotic oral cancer bacteriome.
Conclusion: Oral bacteria may play a role in the early stages of oral carcinogenesis as a dysbiotic bacteriome is associated with oral leukoplakia and this resembles that of oral cancer more than healthy controls. Our findings may have implications for developing oral cancer prevention strategies targeting early microbial drivers of oral carcinogenesis. PMID: 33391629 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - January 6, 2021 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Porphyromonas gingivalis laboratory strains and clinical isolates exhibit different distribution of cell surface and secreted gingipains.
Conclusion: The global P. gingivalis clinical isolates exhibit different Arg- and Lys‑gingipain activities with substantial variability in the level of soluble proteinases released into the environment. PMID: 33391630 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - January 6, 2021 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Performance of at-home self-collected saliva and nasal-oropharyngeal swabs in the surveillance of COVID-19.
Conclusion: Our data show the feasibility of using at-home self-collected samples (especially saliva), as an adequate alternative for SARS-CoV-2 detection. This new approach of testing can be useful to develop strategies for COVID-19 surveillance and for guiding public health decisions. PMID: 33391631 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - January 6, 2021 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Correction Notice.
Authors: Abstract [This corrects the article DOI: 10.1080/20002297.2019.1688449.]. PMID: 33312445 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - December 15, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Correction.
Authors: Abstract [This corrects the article DOI: 10.1080/20002297.2019.1644111.]. PMID: 33312446 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - December 15, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

The use of bacterial DNA from saliva for the detection of GAS pharyngitis.
Conclusion: Saliva-based PCR amplification of GAS DNA method is effective in detection of GAS pharyngitis. Further studies are needed to achieve detection rates to replace the traditional throat swab-based approach. PMID: 33312447 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - December 15, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Correction.
Authors: Abstract [This corrects the article DOI: 10.1080/20002297.2020.1798044.]. PMID: 33312448 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - December 15, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Potential implications of SARS-CoV-2 oral infection in the host microbiota.
Authors: Xiang Z, Koo H, Chen Q, Zhou X, Liu Y, Simon-Soro A Abstract The oral cavity, as the entry point to the body, may play a critical role in the pathogenesis of SARS-CoV-2 infection that has caused a global outbreak of the coronavirus disease 2019 (COVID-19). Available data indicate that the oral cavity may be an active site of infection and an important reservoir of SARS-CoV-2. Considering that the oral surfaces are colonized by a diverse microbial community, it is likely that viruses have interactions with the host microbiota. Patients infected by SARS-CoV-2 may have alterations in the oral and gut microbio...
Source: Journal of Oral Microbiology - December 15, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Isolation and cultivation of candidate phyla radiation Saccharibacteria (TM7) bacteria in coculture with bacterial hosts.
Conclusions: Human oral Saccharibacteria are obligate bacterial parasites that can be stably passaged in coculture with specific species of host bacteria. Isolating these important members of the human oral microbiome, and many natural environments, requires abandoning many of Koch's concepts and methods and embracing novel microbiological approaches. PMID: 33209205 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - November 21, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Susceptibility to biofilm formation on 3D-printed titanium fixation plates used in the mandible: a preliminary study.
Conclusions: The present preliminary study showed that rough surfaces of additively manufactured titanium plates are susceptible to microbial adhesion. The research should be continued in order to compare additively manufactured plates with other commercially available osteotomy plates. Therefore, we suggest caution when using this type of material. PMID: 33194115 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - November 18, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Lactobacillus rhamnosus strains of oral and vaginal origin show strong antifungal activity in vitro.
Authors: Rose Jørgensen M, Thestrup Rikvold P, Lichtenberg M, Østrup Jensen P, Kragelund C, Twetman S Abstract Background: Intake of probiotic bacteria may prevent oral Candida infection. Objective: To screen the antifungal activity of 14 Lactobacillus candidate strains of human origin, against six opportunistic C. albicans and non-albicans species. A second aim was to study the acid production of the four strains showing the strongest antifungal activity. Methods: We used an agar overlay growth inhibition assay to the assess the antifungal activity of the lactobacilli. T...
Source: Journal of Oral Microbiology - November 14, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Investigation of the potential regulator proteins associated with the expression of major surface protein and dentilisin in Treponema denticola.
Authors: Ishihara K, Arai Y, Kikuchi Y, Okamoto-Shibayama K, Kokubu E, Shintani S Abstract Objective Treponema denticola is involved in 'chronic' periodontitis pathogenesis. The mechanism underlying the regulation of the expression of its virulence factors, such as major surface protein (Msp) and prolyl-phenylalanine specific protease (dentilisin) is yet to be clarified. We determined the gene expression profiles of Msp- and dentilisin-deficient mutants of T. denticola to identify the regulation network of gene expression concomitant with the inactivation of these virulence genes. Methods Gene expression profiles o...
Source: Journal of Oral Microbiology - November 7, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Response of Salivary Microbiota to Caries Preventive Treatment in Aboriginal and Torres Strait Islander Children.
Authors: Skelly E, Johnson NW, Kapellas K, Kroon J, Lalloo R, Weyrich L Abstract A once-annual caries preventive (Intervention) treatment was offered to Aboriginal and Torres Strait Islander schoolchildren-a population with disproportionately poorer oral health than non-Indigenous Australian children-in the Northern Peninsula Area (NPA) of Far North Queensland (FNQ), which significantly improved their oral health. Here, we examine the salivary microbiota of these children (mean age = 10 ± 2.96 years; n = 103), reconstructing the bacterial community composition with high-thr...
Source: Journal of Oral Microbiology - November 7, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Production and characteristics of a novel chicken egg yolk antibody (IgY) against periodontitis-associated pathogens.
The objective of this study was to produce an A. actinomycetemcomitans- and P. gingivalis-specific IgY and evaluate its effects on bacterial agglutination and biofilm formation. Thirty laying hens were immunized with a complex of lysate containing typical molecular weights of membrane proteins of A. actinomycetemcomitans and P. gingivalis. IgY was isolated by polyethylene glycol 6000 and ammonium sulfate and purified by dialysis. The results of enzyme-linked immunosorbent assay showed that the obtained IgY were specific to both A. actinomycetemcomitans and P. gingivalis. In addition,...
Source: Journal of Oral Microbiology - November 5, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Activity of five antimicrobial peptides against periodontal as well as non-periodontal pathogenic strains.
Conclusion: AMPs, especially nisin and to a smaller degree lactoferrin, might be promising alternatives to antibiotics because of their antimicrobial activity, high resistance to environmental conditions and partially low costs. PMID: 33133417 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - November 4, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Oral dysbiosis induced by Porphyromonas gingivalis is strain-dependent in mice.
Conclusion: This association between alveolar bone loss and a major persistent shift of the oral microbiota gives insights into virulence discrepancies among these bacterial strains. PMID: 33133418 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - November 4, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Inhibitory effects of 2-methacryloyloxyethyl phosphorylcholine polymer on the adherence of bacteria causing upper respiratory tract infection.
Conclusions: MPC polymer might be effective to reduce the occurrence of upper respiratory tract infection caused by Spn and NTHi and could be applied for the development of local treatments, such as topical gargles and nebulizer medications. PMID: 33062198 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - October 21, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Grade C molar-incisor pattern periodontitis subgingival microbial profile before and after treatment.
Conclusions:Treatment reduced putative disease-associated species, particularly A.a., and shifted the microbial profile to more closely resemble a healthy-site profile. (Clinicaltrials.gov registration #NCT01330719). PMID: 33062199 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - October 21, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Oral Candida albicans colonization in healthy individuals: prevalence, genotypic diversity, stability along time and transmissibility.
In this study, 181 healthy individuals, including 29 couples, were analysed regarding oral yeast colonization using a culture-based approach. Results showed that 39% of the individuals were yeast carriers, 89% being colonized with Candida albicans, 5% with C. guilliermondi, 3% with C. lusitaniae and 3% with C. parapsilosis. Sixty-two percent of the couples had at least one member colonized. Colonization and CFU counts were higher in the couples´ group. Eighty percent of the volunteers were colonized with C. albicans strains with only one CAI genotype, while two but similar CAI genotypes inhabited the oral cavity...
Source: Journal of Oral Microbiology - October 21, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Interaction between genetic factors, Porphyromonas gingivalis and microglia to promote Alzheimer's disease.
Authors: Olsen I, Singhrao SK Abstract In late-onset Alzheimer disease (AD) pathogenesis, genes, infections and immunity could be significant factors. We have reviewed if the keystone periodontal pathogen Porphyromonas gingivalis may affect genes and microglia (primary immune cells in the brain) to promote AD development. Genes for apolipoprotein, clusterin, CD33, triggering receptor expressed on myeloid cells-2 (TREM-2), tyrosine kinase binding protein (TYR-OBP), and complement receptors can affect microglia. Most of these genes can also be affected by P. gingivalis via its mastering of immune suppression. Besides...
Source: Journal of Oral Microbiology - October 21, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Incidence of infective endocarditis caused by viridans group streptococci in Sweden - effect of cessation of antibiotic prophylaxis in dentistry for risk individuals.
Conclusion: The results suggest that the recommended cessation of prophylactic antibiotics for the prevention of IE in dentistry has not led to an increased incidence of IE caused by oral streptococci among the Swedish population. PMID: 33014311 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - October 6, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Phylogenetic diversity in fim and mfa gene clusters between Porphyromonas gingivalis and Porphyromonas gulae, as a potential cause of host specificity.
Conclusions: The differences in the gene clusters between P. gingivalis and P. gulae may result in their host specificity. PMID: 32944148 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 20, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Carbon source utilization patterns in dental plaque and microbial responses to sucrose, lactose, and phenylalanine consumption in severe early childhood caries.
Conclusion: S-ECC plaques had more active interaction with cariogenic carbohydrates like sucrose and lactose than healthy plaques. We supported lactose has less cariogenicity compared with sucrose from microbial community structural aspect. Phenylalanine may have a potentially inhibitory effect on caries development. PMID: 32944149 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 20, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Longitudinal study on oral shedding of human betaherpesviruses 6 and 7 in renal transplant recipients reveals active replication.
Authors: Raposo JV, Sarmento DJS, Pinto RBDS, Lopes AO, Gallottini M, Tozetto-Mendoza TR, Braz-Silva PH, de Paula VS Abstract Backgroung: Roseolovirus latency and persistence in salivary glands that are frequently reactivated after renal transplantation to cause infection have been reported. However, limited information is available on the persistence and excretion of HHV-6 and HHV-7 during and after transplant. Methods: 32 renal transplant recipients were followed up before (T1) and after transplant (T2 and T3) and viral replication (via assessment of mRNA) in oral fluid samples investigated. Roseolovirus...
Source: Journal of Oral Microbiology - September 20, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Mucus is more than just a physical barrier for trapping oral microorganisms.
Authors: Olsen I Abstract Mucus is thought to serve as a protective coating on wet epithelial surfaces. Recent research has shown that glycans, which are branched sugar molecules found in mucin, a part of mucus, can prevent bacteria from communicating with each other and forming biofilms. This could hinder microbes from causing infections. The present editorial, focusing on a paper by Wheeler et al. [1], published in October 2019 in Nature Microbiology, describes how mucus can attenuate the virulence of Pseudomonas aeruginosa. In addition, streptococci and Candida albicans can be 'tamed' by mucin. PMID: 3294415...
Source: Journal of Oral Microbiology - September 20, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Antiviral mouthwashes: possible benefit for COVID-19 with evidence-based approach.
Conclusions: The antiviral mouthwashes play a certainly important role in reducing the viral load of the salivary virus. In the present study, this importance could be proved in two different aspects, that is, the use of mouthwash before dental procedures to reduce the risk of transmission of the virus to the dental team and the use of this mouthwash in COVID-19 patients to help improve systemic problems associated with oral microbial flora. PMID: 32944152 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 20, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Identification and functional analysis of glutamine transporter in Streptococcus mutans.
Conclusions: These results suggest that glnP is associated with glutamine transport in S. mutans, especially the import of glutamine involved in biofilm formation. PMID: 32944153 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 20, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

A Dual Zinc plus Arginine formulation attenuates the pathogenic properties of Porphyromonas gingivalis and protects gingival keratinocyte barrier function in an in vitro model.
Conclusions: The Dual Zinc plus Arginine formulation may offer benefits for patients affected by periodontal disease through its ability to attenuate the pathogenic properties of P. gingivalis and promote keratinocyte barrier function. PMID: 32944154 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 20, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Porphyromonas gingivalis adopts intricate and unique molecular mechanisms to survive and persist within the host: a critical update.
Authors: Chopra A, Bhat SG, Sivaraman K Abstract is an obligate, asaccharolytic, gram-negative bacteria commonly associated with increased periodontal and systemic inflammation. P. gingivalis is known to survive and persist within the host tissues as it modulates the entire ecosystem by either engineering its environment or modifying the host's immune response. It interacts with various host receptors and alters signaling pathways of inflammation, complement system, cell cycle, and apoptosis. P. gingivalis is even known to induce suicidal cell death of the host and other microbes in its vicinity with the emergence ...
Source: Journal of Oral Microbiology - September 20, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Probiotics alter biofilm formation and the transcription of Porphyromonas gingivalis virulence-associated genes.
Conclusions: Probiotics affect P. gingivalis biofilm formation by down-regulating overall PgVAFs with the most pronounced effect observed for L. acidophilus LA5. PMID: 32944156 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 20, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Oral mycobiome identification in atopic dermatitis, leukemia, and HIV patients - a systematic review.
Conclusions: Although morphological and biochemical tests are still used, they are associated with high-throughput sequencing techniques, due to their accuracy and time saving for profiling the predominant species in oral mycobiome. PMID: 32944157 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 20, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Metabolic cooperativity between Porphyromonas gingivalis and Treponema denticola.
Conclusion:  Collectively these metabolic interactions help to partition resources and engage synergistic interactions between these two species. PMID: 32944158 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 20, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Modulation of pathogenic oral biofilms towards health with nisin probiotic.
Conclusion: A nisin-producing probiotic, can be used to treat 'disease-altered' biofilms and promote healthier oral biofilms, which may be useful for improving patient oral health. PMID: 32944159 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 20, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Antifungal effects of Lactobacillus acidophilus and Lactobacillus plantarum against different oral Candida species isolated from HIV/ AIDS patients: an in vitro study.
In this study, the antifungal effects of both cells and cell-free supernatants (CFSs) of L. acidophilus and L. plantarum were investigated against different oral Candida species by co-aggregation, agar overlay interference and broth microdilution assays, respectively. Our results showed that the highest co-aggregation ratio of the two tested Lactic acid bacteria (LAB) was observed for C. krusei. Both L. acidophilus and L. plantarum at cell concentrations 1010 to 102 cfu/ml were able to inhibit the growth of most of the oral Candida species, except for C. albicans, and to some C. krusei. In this study, MIC and MFC values fo...
Source: Journal of Oral Microbiology - September 16, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

A nanocarrier system that potentiates the effect of miconazole within different interkingdom biofilms.
Conclusion: In conclusion, the IONPs-CS-MCZ nanocarrier was efficient against three in vitro models of pathogenic oral biofilms, showing potential to possibly interfere in the synergistic interactions among fungal and bacterial cells within polymicrobial consortia. PMID: 32922677 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 16, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

A case study of salivary microbiome in smokers and non-smokers in Hungary: analysis by shotgun metagenome sequencing.
Conclusion: Smoking-associated dysbiosis of the salivary microbiome in current cigarette smokers, especially increased abundance of Prevotella and Megasphaera genera, may facilitate disease development. PMID: 32922678 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 16, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

A review of co-culture models to study the oral microenvironment and disease.
Authors: Mountcastle SE, Cox SC, Sammons RL, Jabbari S, Shelton RM, Kuehne SA Abstract Co-cultures allow for the study of cell-cell interactions between different eukaryotic species or with bacteria. Such an approach has enabled researchers to more closely mimic complex tissue structures. This review is focused on co-culture systems modelling the oral cavity, which have been used to evaluate this unique cellular environment and understand disease progression. Over time, these systems have developed significantly from simple 2D eukaryotic cultures and planktonic bacteria to more complex 3D tissue engineered structur...
Source: Journal of Oral Microbiology - September 16, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Microbiology of molar-incisor hypomineralization lesions. A pilot study.
Conclusion: The higher protein content of MIH-affected teeth could favour colonization by proteolytic microorganisms. The over-representation of bacteria associated with endodontic infections and periodontal pathologies suggests that, in addition to promote caries development, MIH could increase the risk of other oral diseases. PMID: 32595912 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - July 1, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Lasting Gammaproteobacteria profile changes characterized hematological cancer patients who developed oral mucositis following conditioning therapy.
Conclusions: Post-conditioning OM might contribute to long-term oral microbiome changes affecting Gammaproteobacteria, in HSCT patients. PMID: 32537095 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - June 18, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Dental aerosols: microbial composition and spatial distribution.
Conclusion: Contamination in dental clinics due to aerosols is mainly low, although high level of contamination with taxa from both human and water origin was found within 80 cm around the head of the patient. Our results stress the importance of infection control measures on surfaces in close proximity to the head of the patient as well as in dental water lines. PMID: 32537096 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - June 18, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research