Phylogenetic diversity in fim and mfa gene clusters between Porphyromonas gingivalis and Porphyromonas gulae, as a potential cause of host specificity.
Conclusions: The differences in the gene clusters between P. gingivalis and P. gulae may result in their host specificity. PMID: 32944148 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 20, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Carbon source utilization patterns in dental plaque and microbial responses to sucrose, lactose, and phenylalanine consumption in severe early childhood caries.
Conclusion: S-ECC plaques had more active interaction with cariogenic carbohydrates like sucrose and lactose than healthy plaques. We supported lactose has less cariogenicity compared with sucrose from microbial community structural aspect. Phenylalanine may have a potentially inhibitory effect on caries development. PMID: 32944149 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 20, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Longitudinal study on oral shedding of human betaherpesviruses 6 and 7 in renal transplant recipients reveals active replication.
Authors: Raposo JV, Sarmento DJS, Pinto RBDS, Lopes AO, Gallottini M, Tozetto-Mendoza TR, Braz-Silva PH, de Paula VS Abstract Backgroung: Roseolovirus latency and persistence in salivary glands that are frequently reactivated after renal transplantation to cause infection have been reported. However, limited information is available on the persistence and excretion of HHV-6 and HHV-7 during and after transplant. Methods: 32 renal transplant recipients were followed up before (T1) and after transplant (T2 and T3) and viral replication (via assessment of mRNA) in oral fluid samples investigated. Roseolovirus...
Source: Journal of Oral Microbiology - September 20, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Mucus is more than just a physical barrier for trapping oral microorganisms.
Authors: Olsen I Abstract Mucus is thought to serve as a protective coating on wet epithelial surfaces. Recent research has shown that glycans, which are branched sugar molecules found in mucin, a part of mucus, can prevent bacteria from communicating with each other and forming biofilms. This could hinder microbes from causing infections. The present editorial, focusing on a paper by Wheeler et al. [1], published in October 2019 in Nature Microbiology, describes how mucus can attenuate the virulence of Pseudomonas aeruginosa. In addition, streptococci and Candida albicans can be 'tamed' by mucin. PMID: 3294415...
Source: Journal of Oral Microbiology - September 20, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Antiviral mouthwashes: possible benefit for COVID-19 with evidence-based approach.
Conclusions: The antiviral mouthwashes play a certainly important role in reducing the viral load of the salivary virus. In the present study, this importance could be proved in two different aspects, that is, the use of mouthwash before dental procedures to reduce the risk of transmission of the virus to the dental team and the use of this mouthwash in COVID-19 patients to help improve systemic problems associated with oral microbial flora. PMID: 32944152 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 20, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Identification and functional analysis of glutamine transporter in Streptococcus mutans.
Conclusions: These results suggest that glnP is associated with glutamine transport in S. mutans, especially the import of glutamine involved in biofilm formation. PMID: 32944153 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 20, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

A Dual Zinc plus Arginine formulation attenuates the pathogenic properties of Porphyromonas gingivalis and protects gingival keratinocyte barrier function in an in vitro model.
Conclusions: The Dual Zinc plus Arginine formulation may offer benefits for patients affected by periodontal disease through its ability to attenuate the pathogenic properties of P. gingivalis and promote keratinocyte barrier function. PMID: 32944154 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 20, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Porphyromonas gingivalis adopts intricate and unique molecular mechanisms to survive and persist within the host: a critical update.
Authors: Chopra A, Bhat SG, Sivaraman K Abstract is an obligate, asaccharolytic, gram-negative bacteria commonly associated with increased periodontal and systemic inflammation. P. gingivalis is known to survive and persist within the host tissues as it modulates the entire ecosystem by either engineering its environment or modifying the host's immune response. It interacts with various host receptors and alters signaling pathways of inflammation, complement system, cell cycle, and apoptosis. P. gingivalis is even known to induce suicidal cell death of the host and other microbes in its vicinity with the emergence ...
Source: Journal of Oral Microbiology - September 20, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Probiotics alter biofilm formation and the transcription of Porphyromonas gingivalis virulence-associated genes.
Conclusions: Probiotics affect P. gingivalis biofilm formation by down-regulating overall PgVAFs with the most pronounced effect observed for L. acidophilus LA5. PMID: 32944156 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 20, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Oral mycobiome identification in atopic dermatitis, leukemia, and HIV patients - a systematic review.
Conclusions: Although morphological and biochemical tests are still used, they are associated with high-throughput sequencing techniques, due to their accuracy and time saving for profiling the predominant species in oral mycobiome. PMID: 32944157 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 20, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Metabolic cooperativity between Porphyromonas gingivalis and Treponema denticola.
Conclusion:  Collectively these metabolic interactions help to partition resources and engage synergistic interactions between these two species. PMID: 32944158 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 20, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Modulation of pathogenic oral biofilms towards health with nisin probiotic.
Conclusion: A nisin-producing probiotic, can be used to treat 'disease-altered' biofilms and promote healthier oral biofilms, which may be useful for improving patient oral health. PMID: 32944159 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 20, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Antifungal effects of Lactobacillus acidophilus and Lactobacillus plantarum against different oral Candida species isolated from HIV/ AIDS patients: an in vitro study.
In this study, the antifungal effects of both cells and cell-free supernatants (CFSs) of L. acidophilus and L. plantarum were investigated against different oral Candida species by co-aggregation, agar overlay interference and broth microdilution assays, respectively. Our results showed that the highest co-aggregation ratio of the two tested Lactic acid bacteria (LAB) was observed for C. krusei. Both L. acidophilus and L. plantarum at cell concentrations 1010 to 102 cfu/ml were able to inhibit the growth of most of the oral Candida species, except for C. albicans, and to some C. krusei. In this study, MIC and MFC values fo...
Source: Journal of Oral Microbiology - September 16, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

A nanocarrier system that potentiates the effect of miconazole within different interkingdom biofilms.
Conclusion: In conclusion, the IONPs-CS-MCZ nanocarrier was efficient against three in vitro models of pathogenic oral biofilms, showing potential to possibly interfere in the synergistic interactions among fungal and bacterial cells within polymicrobial consortia. PMID: 32922677 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 16, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

A case study of salivary microbiome in smokers and non-smokers in Hungary: analysis by shotgun metagenome sequencing.
Conclusion: Smoking-associated dysbiosis of the salivary microbiome in current cigarette smokers, especially increased abundance of Prevotella and Megasphaera genera, may facilitate disease development. PMID: 32922678 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 16, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

A review of co-culture models to study the oral microenvironment and disease.
Authors: Mountcastle SE, Cox SC, Sammons RL, Jabbari S, Shelton RM, Kuehne SA Abstract Co-cultures allow for the study of cell-cell interactions between different eukaryotic species or with bacteria. Such an approach has enabled researchers to more closely mimic complex tissue structures. This review is focused on co-culture systems modelling the oral cavity, which have been used to evaluate this unique cellular environment and understand disease progression. Over time, these systems have developed significantly from simple 2D eukaryotic cultures and planktonic bacteria to more complex 3D tissue engineered structur...
Source: Journal of Oral Microbiology - September 16, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Microbiology of molar-incisor hypomineralization lesions. A pilot study.
Conclusion: The higher protein content of MIH-affected teeth could favour colonization by proteolytic microorganisms. The over-representation of bacteria associated with endodontic infections and periodontal pathologies suggests that, in addition to promote caries development, MIH could increase the risk of other oral diseases. PMID: 32595912 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - July 1, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Lasting Gammaproteobacteria profile changes characterized hematological cancer patients who developed oral mucositis following conditioning therapy.
Conclusions: Post-conditioning OM might contribute to long-term oral microbiome changes affecting Gammaproteobacteria, in HSCT patients. PMID: 32537095 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - June 18, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Dental aerosols: microbial composition and spatial distribution.
Conclusion: Contamination in dental clinics due to aerosols is mainly low, although high level of contamination with taxa from both human and water origin was found within 80 cm around the head of the patient. Our results stress the importance of infection control measures on surfaces in close proximity to the head of the patient as well as in dental water lines. PMID: 32537096 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - June 18, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Innovative application of nested PCR for detection of Porphyromonas gingivalis in human highly calcified atherothrombotic plaques.
Authors: Brun A, Rangé H, Prouvost B, Mazighi M, Kapila Y, Bouchard P, Michel JB Abstract Atherothrombosis, leading to stroke and myocardial infarction, is responsible for most of the deaths in the world. An increased risk of atherothrombotic vascular events has been reported in patients with periodontitis. Periodontitis is a chronic multifactorial inflammatory disease, which involves a dysbiotic microbiota, and leads to a progressive destruction of the tooth-supporting apparatus. Transcient periodontal pathogen blood translocation, mainly bacteremia, has been associated with the severity of gingival inflamm...
Source: Journal of Oral Microbiology - May 5, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Co-occurrence of yeast, streptococci, dental decay, and gingivitis in the post-partum period: results of a longitudinal study.
Discussion: C. albicans is associated with active decay and generalized gingivitis, but whether one or both are present depends on the structure of the co-existing microbial community. PMID: 32363007 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - May 5, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Outer membrane vesicle-mediated serum protection in Aggregatibacter actinomycetemcomitans.
Authors: Lindholm M, Metsäniitty M, Granström E, Oscarsson J Abstract Aggregatibacter actinomycetemcomitans belongs to the HACEK group of fastidious Gram-negative organisms, a recognized cause of infective endocarditis. A. actinomycetemcomitans is also implicated in periodontitis, with rapid progress in adolescents. We recently demonstrated that the major outer membrane protein, OmpA1 was critical for serum survival of the A. actinomycetemcomitans serotype a model strain, D7SS, and that the paralogue, OmpA2 could operate as a functional homologue to OmpA1 in mediating serum resistance. In the present work...
Source: Journal of Oral Microbiology - May 5, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

The microbial abundance dynamics of the paediatric oral cavity before and after sleep.
Conclusion: The oral cavity is a dynamic numerically heterogeneous environment where microbial communities can increase by a count of 100 million during sleep. Quantification of the paediatric oral microbiome complements taxonomic diversity information to show how biomass varies and shifts in space and time. PMID: 32341758 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - April 29, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

The relationship between cigarette smoking and the tongue microbiome in an East Asian population.
Conclusions: The tongue microbiomes and related metagenomic pathways of current smokers differ from those of never smokers among East Asians. PMID: 32341759 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - April 29, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Porphyromonas gingivalis W83 traffics via ICAM1 in microvascular endothelial cells and alters capillary organization in vivo.
Conclusions: P. gingivalis perturbs microvascular endothelial function and invasion of these cells via ICAM-1 may be important for microbial persistence within tissues. PMID: 32341760 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - April 29, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Acetaldehyde production by Rothia mucilaginosa isolates from patients with oral leukoplakia.
This study shows that R. mucilaginosa can generate ACH from ethanol in vitro at levels which can induce oxidative stress. This organism likely contributes to oral ACH levels following alcohol consumption and the significance of the increased abundance of R. mucilaginosa in patients with potentially malignant disorders requires further investigation. PMID: 32341761 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - April 29, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Supragingival mycobiome and inter-kingdom interactions in dental caries.
Conclusions: the results substantiate the potential role of the oral mycobiome, primarily Candida species, in dental caries. Inter-kingdom correlations and inhibition of S. mutans by M. globosa are worth further investigation. PMID: 32158514 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - March 13, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

The microbiota of the mother at birth and its influence on the emerging infant oral microbiota from birth to 1 year of age: a cohort study.
Authors: Hurley E, Mullins D, Barrett MP, O'Shea CA, Kinirons M, Ryan CA, Stanton C, Whelton H, Harris HMB, O'Toole PW Abstract Background: The acquisition of microbial communities and the influence of delivery mode on the oral microbiota of the newborn infant remains poorly characterised. Methods: A cohort of pregnant women were enrolled in the study (n = 84). All infants were born full term, by Spontaneous vaginal delivery (SVD) or by Caesarean section (CS). At delivery a saliva sample along with a vaginal/skin sample from the mother. Saliva samples were the taken from the infant within one week of birth, and at ...
Source: Journal of Oral Microbiology - March 6, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

The salivary microbiota in health and disease.
Authors: Belstrøm D Abstract The salivary microbiota (SM), comprising bacteria shed from oral surfaces, has been shown to be individualized, temporally stable and influenced by diet and lifestyle. SM reflects local bacterial alterations of the supragingival and subgingival microbiota, and periodontitis and dental-caries associated characteristics of SM have been reported. Also, data suggest an impact of systemic diseases on SM as demonstrated in patients with a wide variety of systemic diseases including diabetes, cancer, HIV and rheumatoid arthritis. The presence of systemic diseases seems to influence sali...
Source: Journal of Oral Microbiology - March 6, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Candida biome of severe early childhood caries (S-ECC) and its cariogenic virulence traits.
Authors: Fakhruddin KS, Perera Samaranayake L, Egusa H, Chi Ngo H, Panduwawala C, Venkatachalam T, Kumarappan A, Pesee S Abstract The protected niche of deep-caries lesions is a distinctive ecosystem. We assessed the Candida biome and its cariogenic traits from dentin samples of 50 children with severe-early childhood caries (S-ECC). Asymptomatic, primary molars belonging to International Caries Detection and Assessment-ICDAS caries-code 5 and 6 were analyzed, and C. albicans (10-isolates), C. tropicalis (10), C. krusei (10), and C. glabrata (5) isolated from the lesions were then evaluated for their biofilm format...
Source: Journal of Oral Microbiology - March 6, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

High-throughput DNA sequencing of microbiota at interproximal sites.
Conclusion: The microbiota at interproximal regions appears to be specific and stable through time. Potentially pathogenic bacteria may increase caries development risk and gingival inflammation at those sites. PMID: 32002129 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - February 2, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Inhibitory and preventive effects of Lactobacillus plantarum FB-T9 on dental caries in rats.
This study aimed to determine the inhibitory effect of Lactobacillus plantarum FB-T9 on S. mutans biofilm formation in vitro and on the prevention and treatment of dental caries in rats. During in vitro experiments, FB-T9 exhibited good bacteriostatic ability in a plate competition assay. This strain also significantly reduced the biomass and viability of S. mutans biofilms and induced structural damage during the early (6 h), middle (12 h) and late (24 h) stages of biofilm formation. In a 70-day in vivo experiment, FB-T9 significantly reduced the levels of S. mutans on the dental surfaces of rats by more than 2 orders of ...
Source: Journal of Oral Microbiology - February 2, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Integrated hypothesis of dental caries and periodontal diseases.
Authors: Nyvad B, Takahashi N Abstract This review considers an integrated hypothesis of dental caries and periodontal diseases that builds on theoretical ecological principles. The backbone of the hypothesis is based on the dynamic stability stage of the oral microbiota, at which intrinsic (mainly saliva and gingival crevicular fluid) and bacterial (mainly metabolic) resilience factors maintain ecological dynamic stability, compatible with clinical health. However, loss of intrinsic resilience factors and/or prolonged changes in the availability of microbial metabolic substrates may shift the ecological balance of...
Source: Journal of Oral Microbiology - February 2, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Role of EphA2 in host defense against oro-pharyngeal candidiasis.
Authors: Olsen I Abstract Oral defense should be able to sense the burden of, and distinguish between fungal commensals and pathogens, so that an adequate inflammatory response can be set up. Recently, Ephrin type-A receptor 2 (EphA2) was identified on oral epithelial cells and neutrophils that recognizes Candida albicans and induces adaptive protective host responses against this organism. The studies have increased our knowledge of how epithelial cells and neutrophils contribute to host defense against oral yeast infection. PMID: 32002132 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - February 2, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Is there a link between genetic defects in the complement cascade and Porphyromonas gingivalis in Alzheimer's disease?
Authors: Olsen I, Singhrao SK Abstract Defects, as determined by Genome-Wide Association Studies (GWAS), in the complement cascade of innate immunity have been suggested to play a key role in Alzheimer's disease (AD). These defective genes encode sub-component 1s (C1s), complement receptor 1, complement component 9, and clusterin, a fluid-phase regulatory protein. A dysregulated complement cascade has been shown to relate to cell activation, defective complement mediated clearance and possible cognitive decline in AD patients. Porphyromonas gingivalis, a putative keystone pathogen of periodontal disease, has been r...
Source: Journal of Oral Microbiology - January 2, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Shared detection of Porphyromonas gingivalis in cohabiting family members: a systematic review and meta-analysis.
Conclusion: Pooled results suggest that the likelihood of detecting P.gingivalis within within family members is moderately frequent. Personalized periodontal screening and prevention may consider intra-familial co-occurrence of P.gingivalis as feasible. PMID: 31893015 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - January 2, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Evaluating the relationship between acidogenicity and acid tolerance for oral streptococci from children with or without a history of caries.
Conclusion: An ability to grow on acid agar below pH 5.0 is best aligned with strong acidogenicity and best able to distinguish between subjects with differing caries histories. PMID: 31893016 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - January 2, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Protozoans in subgingival biofilm: clinical and bacterial associated factors and impact of scaling and root planing treatment.
Conclusions: Protozoans were associated with some clinical parameters and/or periodontopathogens in patients with periodontitis. PMID: 31893017 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - January 2, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Salivary concentrations of macrophage activation-related chemokines are influenced by non-surgical periodontal treatment: a 12-week follow-up study.
Conclusions: Periodontal treatment impacts salivary concentrations of MCP-2, MDC and MIP-1α, which correlate with the abundance of specific periodontitis-associated bacteria. This indicates that these chemokines reflect periodontal status and possess potential in illustrating a response to treatment. PMID: 31893018 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - January 2, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Oral microbiota and autism spectrum disorder (ASD).
Authors: Olsen I, Hicks SD Abstract Autism spectrum disorder (ASD) is associated with several oropharyngeal abnormalities, including dysbiosis in the oral microbiota. Since the oral cavity is the start of the gastrointestinal tract, this strengthens and extends the notion of a microbial gut-brain axis in ASD and even raises the question whether a microbial oral-brain axis exists. It is clear that oral bacteria can find their way to the brain through a number of pathways following routine dental procedures. A connection between the oral microbiota and a number of other brain disorders has been reported. As the evide...
Source: Journal of Oral Microbiology - January 2, 2020 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Dual-species biofilms of Streptococcus mutans and Candida albicans exhibit more biomass and are mutually beneficial compared with single-species biofilms.
Authors: Lobo CIV, Rinaldi TB, Christiano CMS, De Sales Leite L, Barbugli PA, Klein MI Abstract Background: Streptococcus mutans (Sm) and Candida albicans (Ca) are found in biofilms of early childhood caries. Objective: To characterize in vitro dual- and single-species biofilms of Sm and Ca formed on saliva-coated hydroxyapatite discs in the presence of sucrose. Design: Evaluation of biofilms included biochemical [biomass, proteins, matrix's water-soluble (WSP) and alkali-soluble (ASP) polysaccharides, microbiological, 3D structure, gene expression, and stress tolerance analyses. Results: Biomass and proteins were ...
Source: Journal of Oral Microbiology - November 6, 2019 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Effect of nicotine on biofilm formation of Streptococcus mutans isolates from smoking and non-smoking subjects.
This study was conducted using 60 S. mutans isolates with three S. mutans isolates collected from oral washes of ten smoking subjects and ten from non-smoking subjects. Biofilm was formed by culturing each S. mutans strain (10 μl) in 190 μl of TSB supplemented with 1% sucrose (TSBS) containing 0, 0.25, 0.5, 1.0, 2.0, 4.0, 8.0, 16.0, and 32.0 mg/ml of nicotine for 24 h in 5% CO2 at 37°C in 96 well microtiter plates. The absorbance values of biofilm were measured at 490 nm in a microplate spectrophotometer. Results: There was a significant effect (p-value
Source: Journal of Oral Microbiology - September 27, 2019 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Composition and maternal origin of the neonatal oral cavity microbiota.
Conclusion: The neonatal oral cavity microbiota is not significantly modulated by the birth canal or maternal oral microbiota but displays clear associations with microbes in the placenta. These results suggest that the neonatal oral microbiota may have a prenatal origin. PMID: 31528268 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 20, 2019 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Sex-specific differences in the salivary microbiome of caries-active children.
Conclusion: We have found the acid-generating, cariogenic Lactococcus lactis to be much more abundant in caries-active girls than caries-active boys, indicating that this microorganism may play a more significant role in shaping the cariogenic microbiome in girls. In addition, in caries-active girls, Alloprevotella species HOT 473 was the only species that exhibited both significant sex differences (4.4-fold difference; p=0.0003) as well as high abundance in numbers (1.85% of the total microbial population). PMID: 31497256 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 11, 2019 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Metaproteomics analysis of microbial diversity of human saliva and tongue dorsum in young healthy individuals.
Conclusion: Even if the microbial communities of saliva and tongue dorsum showed a strong similarity based on identified protein functions and deduced bacterial composition, certain specific characteristics were observed. Both microbiomes exhibit a great diversity with seven genera being most abundant. PMID: 31497257 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 11, 2019 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Next-generation sequencing of whole saliva from patients with primary Sj ögren's syndrome and non-Sjögren's sicca reveals comparable salivary microbiota.
Conclusion: The salivary microbiota and oral health status, as well as salivary flow rate in patients with pSS resemble that of non-pSS patients. Our findings indicate that changes in the salivary microbiota do not appear to be determined by the disease entity pSS itself. PMID: 31497258 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 11, 2019 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Mixed aerobic-anaerobic incubation conditions induce proteolytic activity from in vitro salivary biofilms.
Authors: Cleaver LM, Moazzez R, Carpenter GH Abstract Oral biofilms have not been studied using both metabolome and protein profiling concurrently. Bacteria produce proteases that lead to degradation of functional salivary proteins. The novel protocol described here allows for complete characterisation of in vitro oral biofilms, including proteolytic, metabolic, and microbiome analysis. Biofilms were grown on hydroxyapatite discs from whole mouth saliva, using sterilised saliva as a growth-medium, in different growth environments. Salivary protein degradation was assessed from spent saliva ...
Source: Journal of Oral Microbiology - September 7, 2019 Category: Microbiology Tags: J Oral Microbiol Source Type: research

The prevalence and impact of lysogeny among oral isolates of Enterococcus faecalis.
Authors: Stevens RH, Zhang H, Sedgley C, Bergman A, Manda AR Abstract Bacterial phenotypic properties are frequently influenced by the uptake of extrachromosomal genetic elements, such as plasmids and bacteriophage genomes. Such modifications can result in enhanced pathogenicity due to toxin production, increased toxin release, altered antigenicity, and resistance to antibiotics. In the case of bacteriophages, the phage genome can stably integrate into the bacterial chromosome as a prophage, to produce a lysogenic cell. Oral enterococcal strains have been isolated from subgingival plaque and the root canals of endo...
Source: Journal of Oral Microbiology - September 7, 2019 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Toothbrushes may convey bacteria to the cystic fibrosis lower airways.
Conclusion: Toothbrushes may be sources of oral contamination and might act as reservoirs favoring transmission of potentially pathogenic microorganisms from the environment to the oral cavity and eventually to the LAW. Oral hygiene and toothbrush care are important strategies to prevent CF lung infections. PMID: 31489126 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 7, 2019 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Intraoral appliances for in situ oral biofilm growth: a systematic review.
Conclusions: No uniformly superior method for collecting AGOB could be discerned. NGS platforms are preferable for AGOB analyses. PMID: 31489127 [PubMed] (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - September 7, 2019 Category: Microbiology Tags: J Oral Microbiol Source Type: research