Antimicrobial activity of ceftazidime-avibactam and comparators against Pseudomonas aeruginosa and Enterobacterales collected in Croatia, Czech Republic, Hungary, Poland, Latvia and Lithuania: ATLAS Surveillance Program, 2019
AbstractAntimicrobial susceptibility of clinical isolates collected from sites in central Europe in 2019 was tested by CLSI broth microdilution method and EUCAST breakpoints. Most active were amikacin, ceftazidime-avibactam and colistin; respectively, susceptibility rates amongP. aeruginosa (n  = 701) were 89.2%, 92.2% and 99.9%; difficult-to-treat (DTR) isolates, 62.5%, 37.5% and 100%; multidrug-resistant (MDR) isolates, 68.3%, 72.9% and 99.5%; meropenem-resistant (MEM-R), metallo-β-lactamase-negative (MBL-negative) isolates, 72.8%, 78.6% and 100%. AmongEnterobacterales (n = 1639), susceptibility to ceftazidime-a...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 20, 2022 Category: Microbiology Source Type: research

Antimicrobial resistance among Haemophilus influenzae isolates responsible for lower respiratory tract infections in Poland, 2005 –2019
AbstractHaemophilus influenzae is a human-specific pathogen responsible for respiratory tract infections, meningitis, and sepsis. The study aimed to characterize antibiotic resistance inH. influenzae strains isolated from patients with lower respiratory tract infections over 15  years in Poland. The minimum inhibitory concentrations (MICs) of clinically relevant antibiotics were determined by broth microdilution method. Screening for beta-lactam resistance was performed in all isolates following EUCAST recommendation. Finally, relevant changes in penicillin-binding prote in 3 (PBP3) were detected by PCR screening. Of the...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 18, 2022 Category: Microbiology Source Type: research

The absolute number of leukocytes per vial as a major cause of early false positive blood cultures: proof-of-concept and application
AbstractBlood cultures detected as positive by the automated system but negative by microscopy and subculture are considered as “false-positives.” Several causes have been identified, including hyperleukocytosis or the presence of fastidious bacteria, but as many cases remain unexplained we aimed to investigate the false positives occurring in our laboratory. We retrospectively collected data on blood cultures received o ver a period of 12 months to determine factors associated with the false-positive vials. We then prospectively validated our findings on the false-positive results occurring over a 3.5-month period. W...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 18, 2022 Category: Microbiology Source Type: research

Contemporary comparison of infective endocarditis caused by Candida albicans and Candida parapsilosis: a cohort study
AbstractAmong 1655 consecutive patients with infective endocarditis treated from 1998 to 2020 in three tertiary care centres, 16 were caused byCandida albicans (CAIE,n = 8) andCandida parapsilosis (CPIE,n = 8). Compared to CAIE, CPIE were more frequently community-acquired. Prosthetic valve involvement was remarkably more common among patients with CPIE. CPIE cases presented a higher rate of positive blood cultures at admission, persistently positive blood cultures after antifungals initiation and positive valve cultures. All patients but four underwent cardiac surgery. Urgent surgery was more frequently performed in CPIE....
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 14, 2022 Category: Microbiology Source Type: research

Retrospective analysis of outcomes of outpatient parenteral antimicrobial therapy (OPAT) for necrotising otitis externa
This study aims to identify factors associated with treatment response and outcome in patients with NOE treated through outpatient parenteral antimicrobial therapy (OPAT). We performed a retrospective analysis of patients with NOE treated over a 4-year period (January 2018 –January 2022) at a tertiary referral hospital in Derbyshire, UK. We defined OPAT failure as unplanned readmission within 30 days of discontinuation of OPAT. Prolonged duration of therapy was defined as length of parenteral antimicrobial treatment of more than 8 weeks. A total of 46 cases of NOE were reviewed. OPAT failure and prolonged therapy were ...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 13, 2022 Category: Microbiology Source Type: research

Disseminated coccidioidomycosis in immunocompetent patients in non-endemic areas: a case series and literature review
This study aimed to review immunocompetent patients diagnosed with disseminated coccidioidomycosis in a tertiary hospital in Eastern China and other non-endemic areas, and to emphasize the importance of combining travel history with clinical manifestations and proper diagnostic examinations. This study retrospectively reviewed a case series of disseminated coccidioidomycosis diagnosed in an academic hospital in Eastern China. We conducted a global literature review of disseminated coccidioidomycosis in immunocompetent patients with travel history. We identified six patients in our case series and reviewed 42 cases in the l...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 12, 2022 Category: Microbiology Source Type: research

Evolution of Bordetella pertussis in the acellular vaccine era in Norway, 1996 to 2019
We described the population structure ofBordetella pertussis (B. pertussis) in Norway from 1996 to 2019 and determined if there were evolutionary shifts and whether these correlated with changes in the childhood immunization program. We selected 180B. pertussis isolates, 22 from the whole cell vaccine (WCV) era (1996 –1997) and 158 from the acellular vaccine (ACV) era (1998–2019). We conducted whole genome sequencing and determined the distribution and frequency of allelic variants and temporal changes of ACV genes. NorwegianB. pertussis isolates were evenly distributed across a phylogenetic tree that included global s...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 11, 2022 Category: Microbiology Source Type: research

Validation study of an automated chemiluminiscence assay to detect HIV antibodies in oral fluid specimens
AbstractOral fluid specimens (OF) have been widely used to know the HIV prevalence in several key populations. Here, we aim to validate in OF specimens an existing HIV chemiluminiscence assay for serum specimens. Paired OF and serum specimens were collected from 83 known HIV-positives and 83 known HIV-negatives in order to validate the performance characteristics of the automated chemiluminiscence Liaison XL Murex HIV Ag/Ab assay (Diasorin Inc, Iberia) for HIV antibody detection in OF specimens. Among the previously known HIV-seropositive group, HIV antibodies were detected in 69 out of 83 OF specimens. All serum and OF sp...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 7, 2022 Category: Microbiology Source Type: research

Correction to: Validation of Rapid Antimicrobial Susceptibility Testing directly from blood cultures using WASPLab ®, including Colibrí™ and Radian® in-Line Carousel
(Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 5, 2022 Category: Microbiology Source Type: research

Evaluating the efficacy of stool sample on Xpert MTB/RIF Ultra and its comparison with other sample types by meta-analysis for TB diagnostics
This study aims to assess the accuracy of Xpert MTB/RIF Ultra on stool samples and systematically review the performance of Xpert MTB/RIF Ultra with different sample types by meta-analysis. Stool samples of smear-negative pulmonary TB (PTB), cervical lymph node TB, and abdominal TB patients were tested on the Xpert MTB/RIF Ultra system. Meta-analysis was performed on a set of 44 studies. Data were grouped by sample type, and the pooled sensitivity and specificity of Xpert MTB/RIF Ultra were calculated. The sensitivity of Xpert MTB/RIF Ultra with stool samples was 100% for smear-negative PTB, 27.27% for cervical lymph node ...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 4, 2022 Category: Microbiology Source Type: research

Gonococcal tysonitis, a rare local complication of gonorrhea: a clinical study of 15 cases
AbstractTo investigate the incidence, clinical manifestations, and treatments of gonococcal tysonitis in men. We enrolled men with gonococcal tysonitis and men with gonococcal urethritis from January 2000 to December 2020. Demographic data, interval from non-marital sexual contact to the onset of symptoms of gonococcal tysonitis, occurrence sites, and manifestations were recorded for all patients. Ceftriaxone (1  g) was injected intramuscularly once daily for 5 days in patients with lesions comprising abscesses or nodules. A single dose of ceftriaxone (1 g) was injected intramuscularly in patients with sinus-like lesion...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 22, 2022 Category: Microbiology Source Type: research

Prosthetic joint infection diagnosis applying the three-level European Bone and Joint Infection Society (EBJIS) approach
AbstractSensitive and specific tests for the diagnosis of prosthetic joint infection (PJI) are lacking. The aim of this study was to report clinical and microbiological findings of consecutive patients diagnosed with PJI at the University Hospital of Perugia, Perugia, Italy, and to validate these diagnoses utilizing the European Bone and Joint Infection Society (EBJIS) three-level diagnostic approach from 2021. Patients with a PJI diagnosis were included in this study and examined retrospectively. Overall, 133 patients were diagnosed with PJI: mean age 72  years, 54.9% female, and 55.6% with more than one comorbidity. The...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 22, 2022 Category: Microbiology Source Type: research

Prevalence and clinical features of secondary skin lesions in septic patients with bloodstream infections
The objective of this study was to determine the overall prevalence of sepsis-related skin findings and evaluate their possible impact on the prognosis of septic patients. Single-centre, retrospective study of septic patients with documented bloodstream infections admitted in a tertiary hospital during 2019. Primary skin and soft tissue infections, and non-sepsis-related skin conditions diagnosed during hospital admission were excluded. Unselected sample of 320 episodes of sepsis in 265 patients. Secondary skin lesions were documented in 57 sepsis episodes (17.8%) in 47 patients. Purpura (petechiae/ecchymosis) was the most...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 22, 2022 Category: Microbiology Source Type: research

Evaluation of the VITEK2 AST-XN17 card for the detection of carbapenemase-producing Enterobacterales in isolates primarily producing metallo β-lactamase
In this study, we evaluated the detectability of CPE using the results of AST with multiple antimicrobial agents with additional measurements of the AST-XN17 card. In addition, we evaluated the CPE detectability of comments on CPE using the VITEK2 Advance Expert System (AES). In total, 169 Enterobacterales samples, including 76 non-CPE and 93 CPE, collected from multiple medical institutions in the Kinki region of Japan, were used in this investigation. AST with VITEK2 was performed by adding the AST-XN17 card in addition to the AST-N268 or AST-N404 card. Measurement results were identified using cutoff values, primarily C...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 22, 2022 Category: Microbiology Source Type: research

Validation of Rapid Antimicrobial Susceptibility Testing directly from blood cultures using WASPLab ®, including Colibrí™ and Radian® in-Line Carousel
AbstractWith the increase in antimicrobial resistance, fast reporting of antimicrobial susceptibility testing (AST) results is becoming increasingly important. EUCAST developed a method for rapid AST (RAST) directly from the broth of positive blood cultures (BC). Inhibition zones are read after 4, 6, and 8  h, with specific breakpoints per time point. We evaluated the RAST method based on EUCAST disk diffusion methodology with inoculation of BC broth using WASPLab® (inclusive Colibr í™ and Radian®). Forty-nine non-duplicate strains were tested:Escherichia coli n = 17,Klebsiella pneumoniae n = 7,Pseudomonas ae...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 22, 2022 Category: Microbiology Source Type: research