Role of asymptomatic bacteriuria on early periprosthetic joint infection after hip hemiarthroplasty. BARIFER randomized clinical trial
ConclusionsNeither preoperative ASB nor its treatment appears to be risk factors of early-PJI after HHA.ClinicalTrials.gov Identifier: Eudra CT 2016-001108-47 (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 16, 2021 Category: Microbiology Source Type: research

The ID NOW COVID-19, a high-speed high-performance assay
AbstractThe ID NOW COVID-19 assay is a promising tool for the rapid identification of COVID-19 patients. However, its performances were questioned. We evaluate the ID NOW COVID-19 in comparison to a reference RT-PCR using a collection of 48 fresh nasopharyngeal swabs sampled on universal transport media (UTM). Only 2 false negatives of the ID NOW COVID-19 were identified. They display PCR cycle threshold values of 37.5 and 39.2. The positive percent agreement and the negative percent agreement were 94.9% and 100%, respectively. The Kappa value was 0.88. The ID NOW COVID-19 combines high-speed and accurate processing. Using...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 15, 2021 Category: Microbiology Source Type: research

Infective endocarditis caused by HACEK group bacteria —a registry-based comparative study
This study aimed to describe clinical features of IE caused by HACEK genera in comparison with IE due to other pathogens. Cases of IE due to HACEK were identified through the Swedish Registry of Infective Endocarditis (SRIE). Clinical characteristics of IE cases caused by HACEK were compared with cases of IE due to other pathogens reported to the same registry. Ninety-six patients with IE caused by HACEK were identified, and this corresponds to 1.8% of all IE cases. Eighty-three cases were definite endocarditis, and the mortality rate was 2%. The median age was 63 years, which was lower compared to patients with IE caused ...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 14, 2021 Category: Microbiology Source Type: research

Bartonella infections diagnosed in the French reference center, 2014 –2019, and focus on infections in the immunocompromised
AbstractWe studied retrospectively 651 PCR-confirmedBartonella infections diagnosed at the French reference center for bartonellosis from 2014 to 2019. The most common form was cat-scratch disease (89%) followed by endocarditis (9%). Disseminated forms (2%) mainly presented as bacillary angiomatosis or peliosis hepatis in solid organ transplant recipients. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 12, 2021 Category: Microbiology Source Type: research

Accuracy of the serological detection of IgG and IgM to SARS-Cov-2: a prospective, cross-sectional study
AbstractIn response to the rapidly evolving of SARS-CoV-2 infection, numerous serological tests have been developed but their sensitivity and specificity are unclear. We collected serum samples of patients and health-care professionals to assess the accuracy of chemiluminescent (CLIA) and two lateral flow immunochromatographic assays (LFIA) to determine IgG and IgM antibodies to SARS-CoV-2 virus. We calculated theφ correlation for qualitative results and test accuracy, adopting the following case definition: either real-time-PCR positivity or serological positivity with at least two different tests. We analyzed 259 sam...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 10, 2021 Category: Microbiology Source Type: research

Diagnostic value of galactomannan test in non-immunocompromised critically ill patients with influenza-associated aspergillosis: data from three consecutive influenza seasons
AbstractTo explore the diagnostic value of a galactomannan (GM) detection for non-immunocompromised critically ill patients with influenza-associated aspergillosis (IAA). In this retrospective case-control study, we explored the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and area under the receiver operating characteristic (ROC) curve (AUC) of serum and bronchoalveolar lavage fluid (BALF) GM tests by four detection strategies at different detection time points and with different compound modes. In total, 90 patients were evaluated. The AUC values of the second serum GM test,...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 10, 2021 Category: Microbiology Source Type: research

Multicenter evaluation of the VITEK MS matrix-assisted laser desorption/ionization –time of flight mass spectrometry system for identification of bacteria, including Brucella , and yeasts
AbstractThe use of matrix-assisted laser desorption ionization –time of flight (MALDI-TOF) mass spectrometry has proven to be rapid and accurate for the majority of clinical isolates. Some gaps remain concerning rare, emerging, or highly pathogenic species, showing the need to continuously expand the databases. In this multicenter study, we evaluated the accu racy of the VITEK MS v3.2 database in identifying 1172 unique isolates compared to identification by DNA sequence analysis. A total of 93.6% of the isolates were identified to species or group/complex level. A remaining 5.2% of the isolates were identified to th...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 10, 2021 Category: Microbiology Source Type: research

Affinity of Mycobacterium tuberculosis strains for M059K microglial cells after migration through A549 alveolar epithelium
This study evaluated the transmigration ability through the alveolar epithelium and adhesion and invasion capacity of glial cells ofM. tuberculosis strains of varying drug susceptibility and genotype profiles using an in vitro co-culture model. A549 alveolar epithelial cells and M059K glial cells were co-cultured in a Transwell plate with A549 cells cultured in the upper chamber and M059K glial cells in the lower chamber. A549 epithelial cells were infected with F15/LAM4/KZN (susceptible, MDR, XDR), Beijing (susceptible, XDR), F11 (susceptible), F28 (MDR), and H37Rv strains ofM. tuberculosis. The transmigration of an A549 ...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 9, 2021 Category: Microbiology Source Type: research

Fatal Pandoraea nosoerga infection after combined liver-lung transplantation for cystic fibrosis: a recontamination by the pre-transplantation strain
AbstractA 26-year-old girl with a longstanding colonization byPandoraea nosoerga underwent liver-lung transplantation for cystic fibrosis (CF) in 2018. Her brother also suffering from CF was also colonized byP. nosoerga. Despite appropriate perioperative antibiotic therapy, she had post-transplant bacteremic pneumonia caused by extensively drug-resistantP. nosoerga. Drug repurposing was used to optimize treatment options. The cause of post-transplant contamination was studied by comparative whole-genome sequencing including pre- and post-transplant strains and her brother ’s strains. Post-transplant contamination app...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 8, 2021 Category: Microbiology Source Type: research

Bartonella species as a cause of culture-negative endocarditis in South Africa
AbstractPrevious reports have highlighted the high prevalence of blood culture negative endocarditis (BCNE) in South Africa. The Tygerberg Endocarditis Cohort (TEC) study is an ongoing prospective cohort study of patients with confirmed or suspected IE presenting to Tygerberg Academic Hospital, Cape Town, South Africa. Current analysis includes patients that presented between November 2019 and August 2020. Forty four (44) patients have been included in this ongoing study. Fourteen of the 44 patients (31.8%) had BCNE. Further analysis of the patients with BCNE identifiedBartonella species as the most common causative organi...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 7, 2021 Category: Microbiology Source Type: research

Modifications of CD4 T cells, CD4/CD8 ratio and serum levels of soluble CD14 in HIV-HCV-coinfected patients after sustained HCV response induced by direct-acting antiviral agents: influence of liver cirrhosis
AbstractTo analyze the modifications of CD4 T cell, CD4/CD8 ratio, and serum levels of soluble CD14 (sCD14) in HIV/HCV-coinfected patients after treatment with direct anti-HCV antiviral agents. Consecutive cases of HIV/HCV-coinfected patients, attended at the University Hospital, who achieved sustained virological responses with interferon-free hepatitis C antiviral drugs, were analyzed. Thirty-five percent of patients (n = 39) had been diagnosed with liver cirrhosis. The evaluation criteria were changes in CD4 T-cell counts and percentages and inflammation (measured by serum sCD14 levels) or immune activation indexes (det...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 6, 2021 Category: Microbiology Source Type: research

Carriage of multiple Streptococcus pneumoniae capsular types is frequent among children with invasive pneumococcal disease
This study reports a high frequency of pneumococcal multiple serotype carriage in children with invasive pneumococcal disease. Whether multiple serotype carriage is important for the onset and progress to pneumococcal infection warrants further investigation. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 2, 2021 Category: Microbiology Source Type: research

Exploratory development of PCR-fluorescent probes in rapid detection of mutations associated with extensively drug-resistant tuberculosis
This study aims to evaluate the clinical value of PCR-fluorescent probes for detecting the mutation gene associated with extensively drug-resistant tuberculosis (XDR-TB). The molecular species identification of 900 sputum specimens was performed using polymerase chain reaction (PCR) –fluorescent probe. The mutations of the drug resistance genesrpoB,katG,inhA,embB,rpsL,rrs, andgyrA were detected. The conventional drug susceptibility testing (DST) and PCR-directed sequencing (PCR-DS) were carried out as control. DST demonstrated that there were 501 strains of rifampicin resistance, 451 strains of isoniazid resistance, ...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 1, 2021 Category: Microbiology Source Type: research

Reducing the dosing frequency of selective digestive tract decontamination to three times daily provides effective decontamination of Gram-negative bacteria
This study evaluated the effectiveness of selective digestive tract decontamination (SDD) application three times daily (t.i.d.) compared to the standard four times daily (q.i.d.). Retrospective equivalence (combined non-inferiority and non-superiority design) study with a before-and-after design on a tertiary ICU in which the SDD frequency was reduced from q.i.d. to t.i.d. All patients with ICU admissions ≥72h and with ≥2 surveillance cultures collected on different dates were included in this study. We compared successful decontamination of Gram-negative bacteria (GNB). Furthermore, time to decontamination, ICU-acq...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 1, 2021 Category: Microbiology Source Type: research

Rifampin-accompanied antibiotic regimens in the treatment of prosthetic joint infections: a frequentist and Bayesian meta-analysis of current evidence
This study aimed to combine current evidence supporting the rifampin supplement to an effective antibiotic in the treatment of prosthetic joint infections. We conducted a random-effects meta-analysis with frequentist and Bayesian approaches. A total of 13 studies, all observational, were included in the final analysis. The predominant bacteria in eight, two, and three studies wereStaphylococcus spp.,Propionibacterium spp., andStreptococcus spp., respectively. We pooled data from 568 patients in the staphylococcus subset (OR, 1.18; 95% CIs, [0.76; 1.82];I2 = 23%) and data from 80 patients in the propionibacterium subset (RE...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 20, 2021 Category: Microbiology Source Type: research

Sensitive detection and quantification of SARS-CoV-2 by multiplex droplet digital RT-PCR
AbstractThe purpose of this study is to develop a one-step droplet digital RT-PCR (RT-ddPCR) multiplex assay that allows for sensitive quantification of SARS-CoV-2 RNA with respect to human-derived RNA and could be used for screening and monitoring of Covid-19 patients. A one-step RT-ddPCR multiplex assay was developed for simultaneous detection of SARS-CoV-2E, RdRp andN viral RNA, and humanRpp30 DNA andGUSB mRNA, for internal nucleic acid (NA) extraction and RT-PCR control. Dilution series of viral RNA transcripts were prepared in water and total NA extract of Covid-19-negative patients. As reference assay, anE-GUSB duple...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 20, 2021 Category: Microbiology Source Type: research

Microbiological profiles and antimicrobial resistance patterns of pediatric bloodstream pathogens in China, 2016 –2018
This study aimed to investigate the microbiological profiles and antimicrobial resistance patterns of bloodstream pathogens in Chinese children.MethodsThis retrospective study was conducted at 13 tertiary hospitals in China during 2016 –2018. The first bloodstream isolates of the same species from one pediatric patient
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 20, 2021 Category: Microbiology Source Type: research

Prevalence and mechanisms of linezolid resistance among staphylococcal clinical isolates from Egypt
AbstractThe emergence of methicillin-resistant staphylococci necessitated the search for alternative agents as linezolid, introduced to treat infections due to multidrug-resistant bacteria. Linezolid resistance has since emerged, yet its global prevalence remains low. In Egypt, little is known about the situation. We investigated the prevalence and mechanisms of resistance among Egyptian staphylococcal clinical isolates. Linezolid resistance among 232 staphylococcal isolates obtained from Alexandria Main Hospitals between 2011 and 2016 was assessed using disc diffusion and minimum inhibitory concentration. Resistant isolat...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 20, 2021 Category: Microbiology Source Type: research

Prevalence of genital HPV infection in STI and healthy populations and risk factors for viral persistence
This study aimed to assess prevalence and persistence rate of genital HPV infection in sexually transmitted infections (STIs) patients and healthy subjects. The risk factors influencing the persistence of genital HPV infection were also investigated. The samples were collected with the ThinPrep liquid-based cytology system. Among the HPV-positive patients, those consenting were retested after 12  months. Overall, 145/292 subjects proved HPV positive with a higher prevalence (51%) in STI than in healthy population (43%). The persistence of genital HPV infection was statistically associated with female gender, HR-HPV in...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 20, 2021 Category: Microbiology Source Type: research

Delayed hospital admission and high-dose corticosteroids potentially prolong SARS-CoV-2 RNA detection duration of patients with COVID-19
AbstractCoronavirus disease 2019 (COVID-19) with the infection of SARS-CoV-2 has become a serious pandemic worldwide. However, only few studies focused on risk factors of prolonged SARS-CoV-2 RNA detection among patients with COVID-19. We included 206 adult patients with laboratory-confirmed COVID-19 from two hospitals between 23 Jan and 1 April 2020. Least absolute shrinkage and selection operator (LASSO) analysis was used to screen out independent risk factors of SARS-CoV-2 RNA detection. By multivariate binomial logistic regression analysis and Cox regression analysis, we further determined the associations between SARS...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 20, 2021 Category: Microbiology Source Type: research

High versus standard doses of corticosteroids in severe COVID-19: a retrospective cohort study
AbstractDespite the increasing evidence of the benefit of corticosteroids for the treatment of moderate-severe coronavirus disease 2019 (COVID-19) patients, no data are available about the potential role of high doses of steroids for these patients. We evaluated the mortality, the risk of need for mechanical ventilation (MV), or death and the risk of developing a severe acute respiratory distress syndrome (ARDS) between high (HD) and standard doses (SD) among patients with a severe COVID-19. All consecutive confirmed COVID-19 patients admitted to a single center were selected, including those treated with steroids and an A...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 20, 2021 Category: Microbiology Source Type: research

Description of an influenza outbreak in a French university hospital and risk factors of nosocomial influenza
AbstractOur objective was to evaluate risk factors of nosocomial influenza (NI) in an university hospital during the 2015/2016 influenza season. All hospitalized patients with influenza-like illness associated with laboratory confirmation by polymerase chain reaction were included in a prospective observational study. We identified 44 cases (19%) of NI among the 233 cases of influenza: 38/178 (21%) in adults and 6/55 (11%) in children. Among adults, hospitalization in a double or multi-occupancy room was independently associated with NI (adjusted Odds Ratio, 3.42; 95% CI, 1.29 –9.08;p = 0.013). The results of the stu...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 20, 2021 Category: Microbiology Source Type: research

Rapid detection of antimicrobial resistance markers with Allplex ™ Entero-DR assay directly from positive blood culture bottles
AbstractA method for rapid detection of one extended-spectrum β-lactamase (ESBL) and five carbapenemase-encoding genes as well as vancomycin resistance markers directly from blood cultures using the Allplex™ Entero-DR assay (Seegene, Seoul, South Korea) is presented. Altogether 28 previously well-characterized resistant Gram-negative bacilli andEnterococcus spp., and 142 clinical blood cultures containing Gram-negative bacilli or Gram-positive cocci were analyzed. The method had 100% sensitivity and specificity for detectingblaOXA-48-like,blaKPC,blaVIM,blaIMP,blaNDM,blaCTX-M,vanA, andvanB. The lowest detectable ...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 20, 2021 Category: Microbiology Source Type: research

Bloodstream infection due to Herbaspirillum sp.: case series and review of literature
We describe our own case series and review the cases reported in the literature. Cancer appears as the major underlying disease. The main source of bacteraemia was respiratory. Phenotypic identification methods often misidentify this species. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI –TOF MS) and molecular methods identify at genus level, but species assignment is not reliable.Herbaspirillum spp. showed a highly susceptible antimicrobial profile. β-Lactams showed good activity with low MIC values, except ampicillin. All isolates were resistant to colistin, suggesting an ...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 20, 2021 Category: Microbiology Source Type: research

Epidemiological of cat scratch disease among inpatients in the Spanish health system (1997 –2015)
AbstractCat scratch disease, whose causative agent isBartonella henselae, is an anthropozoonosis with a worldwide distribution that causes significant public health problems. Although it is an endemic disease in Spain, the available data are very limited. The aim of our study was to describe cat scratch disease inpatients in the National Health System (NHS) of Spain. This was a retrospective descriptive study using the minimum basic data set (CMBD in Spanish) in patients admitted to hospitals of the NHS between 1997 and 2015 with a diagnosis of cat scratch disease (ICD-9: 078.3). We found 781 hospitalized patients diagnose...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 20, 2021 Category: Microbiology Source Type: research

Multi-year prevalence and macrolide resistance of Mycoplasma genitalium in clinical samples from a southern Italian hospital
AbstractThe use of azithromycin for the treatment ofMycoplasma genitalium infections has led to resistance to macrolides. From July 2014 to July 2020, 7150 samples were analysed for the detection of sexually transmitted infections at the Policlinico of Bari. A total of 67/7150 samples (0.93%) were positive for MG DNA and 47 samples were analysed for the evaluation of six azithromycin resistance-associated mutations. In 5/47 samples, the A2058G mutation was detected (10.63%). Although the cases of positive MG samples and mutations are low in our reality, diagnostic tests to detect azithromycin resistant-associated genes may...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 20, 2021 Category: Microbiology Source Type: research

Genetic and phenotypic characteristics of mcr-8 positive extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolated from patient in China
(Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 20, 2021 Category: Microbiology Source Type: research

Pooling of samples to optimize SARS-CoV-2 diagnosis by RT-qPCR: comparative analysis of two protocols
This study has aimed to evaluate the use pool of samples as a strategy to optimize the diagnostic of SARS-CoV-2 by RT-qPCR. A total of 220 naso/orofaryngeal swab samples were collected and tested using two different protocols of sample pooling. Results from protocol A were identical with the individual results. However, for results from protocol B, reduced agreement (91%) was observed in relation to individual testing. Inconsistencies observed were related to RT-qPCR results with higher cycle thresholds. These results suggest that pooling of samples before RNA extraction is preferable in terms of diagnostic for SARS-CoV-2....
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 20, 2021 Category: Microbiology Source Type: research

Evaluation of three immunochromatographic tests in COVID-19 serologic diagnosis and their clinical usefulness
AbstractResults of three rapid immunochromatographic tests (ICTs) were compared with those obtained with two automated immunoassays for evaluation of their usefulness. One hundred fifty-nine patients and 67 healthy volunteers were included. Different assays demonstrate 41 –45% of diagnostic sensitivities and 91–98% of specificities, with substantial agreement (89.3–91.2%), but a high percentage of weak positive results (13–22%) was observed with ICTs. ICTs performances were comparable to those of automated immunoassays. ICTs could have a role as screening app roach due to their easy usability. Subje...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 20, 2021 Category: Microbiology Source Type: research

Clinical characteristics of SARS-CoV-2 by re-infection vs. reactivation: a case series from Iran
In this study, we investigated three COVID-19 patients who represented the symptoms after recovery. Chest CT scan was applied to assess the patients along with the viral samples from oropharyngeal/nasopharyngeal which were subjected to RT-PCR. The viral genome sequencing was applied where possible to distinguish possible re-infection or latent reactivation. Moreover, COVID-19-specific antibodies available data were evaluated in each incidence. The second episode of SARS-CoV-2 infection was different among the investigated subjects who experienced an interval between positive PCR tests ranged between 63 and 156 days. The di...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 18, 2021 Category: Microbiology Source Type: research

Evaluation of the CARDS toxin and its fragment for the serodiagnosis of Mycoplasma pneumoniae infections
In this study, the full-length, N-terminal, and C-terminal regions of the CARDS toxin were expressed and purified, and serological reactions were evaluated using ELISA. A total of 184 serum samples were collected and tested using a commercialized test kit. Eighty-seven samples were positive, and 97 samples were negative for infection. The purified recombinant proteins were used as antigens to test the serum via indirect ELISA. The sensitivity of the CARDS toxin, the N-terminal region, and the C-terminal region were 90.8%, 90.8%, and 92.0%, respectively. The specificity of the CARDS toxin, the N-terminal region, and the C-t...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 18, 2021 Category: Microbiology Source Type: research

Evaluation of 18 commercial serological assays for the detection of antibodies against SARS-CoV-2 in paired serum samples
AbstractA variety of serological tests have been developed to detect the presence of antibodies against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We evaluated the performance of 18 commercially available SARS-CoV-2 antibody assays. Early (6 –8 days after the start of symptoms) and late sera (>14 days) from ICU patients (n=10 andn=16, respectively) and healthcare workers (n=5 andn=9, respectively) were included. Additionally, 22 sera were included to detect potential cross-reactivity. Test characteristics were determined for the 18 assays. In>14 days samples, the Vircell IgG and Wantai Ig...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 17, 2021 Category: Microbiology Source Type: research

Impact of rapid multiplex PCR on management of antibiotic therapy in COVID-19-positive patients hospitalized in intensive care unit
AbstractBecause the diagnosis of co/superinfection in COVID-19 patients is challenging, empirical antibiotic therapy is frequently initiated until microbiological analysis results. We evaluated the performance and the impact of the BioFire ® FilmArray® Pneumonia plus Panel on 112 respiratory samples from 67 COVID-19 ICU patients suspected of co/superinfections. Globally, the sensitivity and specificity of the test were 89.3% and 99.1%, respectively. Positive tests led to antibiotic initiation or adaptation in 15% of episodes and de- escalation in 4%. When negative, 28% of episodes remained antibiotic-free (14% no i...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 17, 2021 Category: Microbiology Source Type: research

Bone and joint infections caused by Clostridium perfringens: a case series
The objective of this study was to evaluate antimicrobial therapy outcomes of bone and joint infections (BJI) caused byClostridium perfringens. We investigated remission of symptoms and the absence of relapse or reinfection during follow-up. Among the 8 patients withC. perfringens BJI, the type of infection was early prosthesis infection (n = 2), osteosynthetic device infection (n = 4), and chronic osteomyeletis (n = 2). Clindamycin-rifampicin combination was given in 4 cases and metronidazole in 4 cases. The overall success rate was 87.5%. Among the 7 patients who completed antibi...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 16, 2021 Category: Microbiology Source Type: research

Comparison between Abbott m2000 RealTime and Alinity m STI systems for detection of Chlamydia trachomatis , Neisseria gonorrhoeae , and Mycoplasma genitalium
AbstractThe new Abbott Alinity m STI Assay was compared with Abbott m2000 RealTime PCR. ForChlamydia trachomatis, 26 (7.5%) of 347 samples were positive in the Alinity assay and 24 (6.9%) in the m2000 assay. Corresponding figures forNeisseria gonorrhoeae were 23 (6.6%) and 17 (4.9%). ForMycoplasma genitalium, 22 (7.9%) of 279 samples were positive in the Alinity assay and 18 (6.5%) in the m2000 assay, for which DNA extraction was performed on an m2000sp instrument combined with in-house real-time PCR. The Alinity assay has at least the same sensitivity as the m2000 assay. The specificity was evaluated by discrepancy analys...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 15, 2021 Category: Microbiology Source Type: research

Vulvovaginal candidiasis and current perspectives: new risk factors and laboratory diagnosis by using MALDI TOF for identifying species in primary infection and recurrence
This study analyzed 278 patients, divided into symptomatic (n = 173) and asymptomatic (n = 105) groups. Regarding the main candidiasis symptoms (discharge, itching, and burning), only 50.3% of patients described these concomitant symptoms, showing a positive predictive value of 67.8%. Regarding the risk factors investigated, there was a statistical correlation between candidiasis and dairy products, gut transit, contraceptive use, respiratory allergy, and panty liners, describing new risk factors related to intestinal and vaginal dysbiosis. AfterCandida species analysis and confirmation, the primary prevalence was 80.9% (C...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 13, 2021 Category: Microbiology Source Type: research

Do-not-intubate status and COVID-19 mortality in patients admitted to Dutch non-ICU wards
AbstractMortality from COVID-19 has been particularly high in elderly patients on mechanical ventilation. Treatment outcomes for patients with do-not-intubate (DNI) status are unknown. One hundred patients admitted to the non-ICU ward during the “first wave” were retrospectively analyzed. Mortality rate was 49% in patients with a DNI order. This subgroup was characterized by significantly higher age, more comorbidity, and care dependency. Mortality among DNI patients was three times higher than other patients, but not higher than some o f the published mortality rates for elderly mechanically ventilated patient...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 12, 2021 Category: Microbiology Source Type: research

Ceftolozane/tazobactam for Pseudomonas aeruginosa pulmonary exacerbations in cystic fibrosis adult patients: a case series
We describe our experience in the use of C/T forP. aeruginosa-related pulmonary exacerbations (PE) in CF adults admitted within 2016 and 2019 at Careggi Hospital, Florence, Italy. PE was diagnosed as deterioration of respiratory function, worsening cough, and increasing of sputum. C/T was given at the dose of 3 g every 8 h. C/T was used in ten patients. Mean length of C/T treatment was 16.3 days, and tobramycin was the most frequently combined antipseudomonal agent. All patients were successfully treated although susceptibility testing on sputum sample showed C/T resistance in two cases. No adverse effects related to C/T w...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 12, 2021 Category: Microbiology Source Type: research

Direct next-generation sequencing diagnosis of echovirus 9 meningitis, France
In this study, next-generation sequencing identified an echovirus 9 directly from the cerebrospinal fluid of a patient presenting with meningitis. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 11, 2021 Category: Microbiology Source Type: research

Long-term use of liposomal nebulized amikacin and tedizolid for the treatment of disseminated nocardiosis after allogeneic hematopoietic stem cell transplantation
AbstractNocardiosis is a life-threatening opportunistic infection in immunocompromised patients. Herein, we present successful adjunctive use of liposomal nebulized amikacin and tedizolid in a recipient of allogeneic hematopoietic stem cell transplantation infected withNocardia nova complex who presented multiple complications to conventional therapeutic options. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 10, 2021 Category: Microbiology Source Type: research

Attributable mortality of ICU acquired bloodstream infections: a propensity-score matched analysis
AbstractThe mortality attributable to ICU-acquired bloodstream infection (BSI) differs between studies due to statistical methods used for cohort matching. Propensity-score matching has never been used to avoid eventual bias when studying BSI attributable mortality in the ICU. We conducted an observational prospective study over a 4-year period, on patients admitted for at least 48 h in 2 intensive care units. Based on risk factors for death in the ICU and for BSI, each patient with BSI was matched with 3 patients without BSI using propensity-score matching. We performed a competitive risk analysis to study BSI mortality a...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 10, 2021 Category: Microbiology Source Type: research

Bloodstream infections in older population: epidemiology, outcome, and impact of multidrug resistance
This study is a retrospective cohort of all BSI episodes occurring among older patients (> 65yo), from April 1, 2010, to December 31, 2016, in a French university hospital for geriatric medicine. A total of 255 BSI episodes were analyzed. Mean age was 86.3 ±6.5 years, and sex ratio (M/F) was 0.96. Main comorbidities were orthopedic device (26.7%), active neoplasia (24.3%), and diabetes mellitus (18.4%). Main primary sites of infection were urinary tract infections (56.9%), respiratory tract infections (10.6%), intra-abdominal infections (7.1%), and s kin and soft tissue infections (4.7%). Main bacteria identified...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 10, 2021 Category: Microbiology Source Type: research

Incidence, clinical characteristics, risk factors and outcomes of meningoencephalitis in patients with COVID-19
AbstractWe investigated the incidence, clinical characteristics, risk factors, and outcome of meningoencephalitis (ME) in patients with COVID-19 attending emergency departments (ED), before hospitalization. We retrospectively reviewed all COVID patients diagnosed with ME in 61 Spanish EDs (20% of Spanish EDs, COVID-ME) during the COVID pandemic. We formed two control groups: non-COVID patients with ME (non-COVID-ME) and COVID patients without ME (COVID-non-ME). Unadjusted comparisons between cases and controls were performed regarding 57 baseline and clinical characteristics and 4 outcomes. Cerebrospinal fluid (CSF) bioche...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 9, 2021 Category: Microbiology Source Type: research

Short time to blood culture positivity in Enterococcus faecalis infective endocarditis
AbstractTime to blood culture positivity (TTP) is an indirect measure of bacterial concentration in blood. A short TTP has been linked to the presence of infective endocarditis (IE) and to poor prognosis inStaphylococcus aureus bacteremia. We analyze factors influencing TTP in bacteremia withEnterococcus faecalis. This retrospective observational study of medical records included adults diagnosed with monomicrobialE.faecalis bacteremia between 2015 and 2018 in the Sk åne region (Sweden). For each episode, the shortest TTP was recorded. Median TTP was compared between patients grouped based on age, sex, comorbidity, s...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 9, 2021 Category: Microbiology Source Type: research

COVID-19 or not COVID-19? Compared characteristics of patients hospitalized for suspected COVID-19
AbstractDuring an epidemic period, we compared patients hospitalized for initial suspicion of COVID-19 but for whom an alternative diagnosis was finally retained (n = 152) with those who had COVID-19 (n = 222). Most common diagnoses were another infectious disease and heart failure. COVID-19-negative patients were more often active smokers had less often cough, fever, and digestive symptoms, as compared to the 222 COVID-19-positive patients. They had higher median neutrophil and lymphocyte counts and lower CRP level. In multivariate analysis, no current smoking, neurocognitive disorder, myalgia, and fibrinogen ≥4g/L wer...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 8, 2021 Category: Microbiology Source Type: research

Characterization of an osteomyelitis case caused by dalbavancin, ceftaroline, and vancomycin non-susceptible methicillin-resistant Staphylococcus aureus
We report a case of osteomyelitis due to methicillin-resistantStaphylococcus aureus (MRSA) that is also non-susceptible to vancomycin, dalbavancin, ceftaroline, and ceftobiprole, in the absence of exposure to the latter three antibiotics. It was isolated from a patient with a 26-year history of cranial surgeries and episodes of osteomyelitis. Whole-genome sequencing was performed. It was found to belong to ST247 and themecA gene was detected within the SSCmec type I (1B) gene cassette that lacked the E447K mutation known to produce resistance to ceftobiprole and ceftaroline. However, mutations in other genes related to res...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 8, 2021 Category: Microbiology Source Type: research

Comparison of clinical characteristics and outcomes between respiratory syncytial virus and influenza-related pneumonia in China from 2013 to 2019
This study aims to compare clinical characteristics and severity between adults with respiratory syncytial virus (RSV-p) and influenza-related pneumonia (Flu-p). A total of 127 patients with RSV-p, 693 patients with influenza A-related pneumonia (FluA-p), and 386 patients with influenza B-related pneumonia (FluB-p) were retrospectively reviewed from 2013 through 2019 in five teaching hospitals in China. A multivariate logistic regression model indicated that age ≥ 50 years, cerebrovascular disease, chronic kidney disease, solid malignant tumor, nasal congestion, myalgia, sputum production, respiratory rates ≥ 30 beats/min, lymphocytes
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 6, 2021 Category: Microbiology Source Type: research

Therapeutic efficacy of chitosan nanoparticles loaded with BCG-polysaccharide nucleic acid and ovalbumin on airway inflammation in asthmatic mice
In this study, immunoregulation and desensitization therapies were jointly applied in the treatment of asthma, in which chitosan (CS) nanoparticles were used. BALB/c mice were selected and mouse models of asthma were constructed. Mice were divided into 7 groups. A double-chamber plethysmograph, MTT, hematoxylin-eosin staining, and ELISA were used. The expression levels of IL-4 and IL-5 in lung tissue cells were detected. CS-BCG-PSN-OVA sustained-release vaccines significantly alleviated airway hyperresponsiveness (AHR) in asthmatic mice. The numbers of total lymphocytes and eosinophils in BALF were remarkably reduced. The ...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 5, 2021 Category: Microbiology Source Type: research

The use of multi-pronged screening strategy to understand the epidemiology of carbapenemase-producing Enterobacteriaceae in Hong Kong: transition from epidemic to endemic setting
AbstractA multi-pronged carbapenemase-producing Enterobacteriaceae (CPE) screening strategy was implemented in Hong Kong West healthcare network. Of 199,192 fecal specimens from 77,194 patients screening from 1 July 2011 to 30 June 2019, the incidence of CPE per 1000 patient admission significantly increased from 0.01 (2012) to 1.9 (2018) (p3 times per week significantly associated with community acquisition of CPE. Strategic CPE control measures should be implemented in both the hospital and the community. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 5, 2021 Category: Microbiology Source Type: research

The Unyvero Hospital-Acquired pneumonia panel for diagnosis of secondary bacterial pneumonia in COVID-19 patients
In conclusion, the HPN Application demonstrated higher diagnostic yield in comparison with the culture and generated results within 5 h. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 4, 2021 Category: Microbiology Source Type: research