European Journal of Clinical Microbiology and Infectious Diseases This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Simultaneous gut colonization by Klebsiella grimontii and Escherichia coli co-possessing the blaKPC-3-carrying pQil plasmid
AbstractOnly two plasmid-mediated carbapenemases (KPC-2 and VIM-1) are reported inKlebsiella grimontii. Here, we report twoblaKPC-3-positive isolates that were identified asK. oxytoca andE. coli by MALDI-TOF MS in the same rectal swab. Whole-genome sequencing indicated thatK. oxytoca was actuallyK. grimontii of ST391, whereasE. coli was of ST10. In both,blaKPC-3 was carried by a pQil conjugative plasmid. The core-genome analysis identified additionalblaKPC-positiveK. grimontii strains from public databases, most of which were misidentified asK. oxytoca. SinceK. grimontii represents an emerging reservoir of resistance trait...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 28, 2022 Category: Microbiology Source Type: research

Diagnostic accuracy of the metagenomic next-generation sequencing (mNGS) for detection of bacterial meningoencephalitis: a systematic review and meta-analysis
This study aims to evaluate and compare the diagnostic efficacy of mNGS for the diagnosis of BM/E using cerebrospinal fluid (CSF) specimens versus a composite reference standard (CRS). The electronic databases (Embase, PubMed, and Web of Science) were searched up to 15 June 2021. Studies such as cohort, case –control, prospective, or retrospective studies that assessed the diagnostic efficacy of mNGS in suspected bacterial meningitis/encephalitis cases were included. Ten studies met the inclusion criteria, including three retrospective and seven prospective studies. The sensitivity of mNGS for diagnos is of BM/E from CSF...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 27, 2022 Category: Microbiology Source Type: research

Automation of RIDA ®GENE Helicobacter pylori PCR on the BD MAX™ System
AbstractPCR detection ofHelicobacter pylori infection in gastric biopsies allows the detection of this bacterium and the mutations associated with macrolide resistance. The aim of this study was to evaluate the performance of RIDA ®GENEH. pylori PCR (r-Biopharm) on a BD MAX ™ System (Becton Dickinson). Two hundred ten gastric biopsies obtained were included. These biopsies were ground in nutrient broth. Two hundred microliters of this suspension was treated with proteinase K; 200 µL was transferred to a BD MAX™ sample tube then tested using RIDA®GENEH. pylori PCR reagents. In-houseH. pylori PCR was used as a referen...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 27, 2022 Category: Microbiology Source Type: research

Streptococcus pneumoniae colonization in health care professionals at a tertiary university pediatric hospital
AbstractStreptococcus pneumoniae is a commensal of the human upper respiratory tract. In certain cases, it can lead to serious invasive infections peaking in very young children and the elderly. Especially young children are frequent carriers and are thus regarded as the reservoir for horizontal transmission of pneumococci. This is the first study evaluating pneumococcal colonization patterns in healthcare professionals working in a tertiary care pediatric hospital, including carriage prevalence, serotype distribution, and risk factors for carriage. One oropharyngeal and one nasal swab per individual were directly plated o...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 27, 2022 Category: Microbiology Source Type: research

Dalbavancin in clinical practice: a particular place for the elderly?
AbstractWe investigate dalbavancin efficiency and tolerance among elderly in Grenoble-Alpes 32 university hospital. Among the 65 patients who received dalbavancin, 51% (33) were considered as old. Patients presented mainly bones and joint infections (52%), surgical site infection 34 (31%), and infective endocarditis (IE) (8%). Clinical cure was confirmed for 79% of old 35 patients at 1, 3, and 6  months. Six adverse events (9%) were reported after 36 dalbavancin’s administration, but each time in combination with other antibiotics. 37 Dalbavancin had a significant effectiveness and safety profile and represents a real 3...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 27, 2022 Category: Microbiology Source Type: research

Rapid increase in SARS-CoV-2 seroprevalence during the emergence of Omicron variant, Finland
(Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 27, 2022 Category: Microbiology Source Type: research

Use of DosR and Rpf antigens from Mycobacterium tuberculosis to screen for latent and relapse tuberculosis infection in a tuberculosis endemic community of Huainan City
AbstractThe dormancy survival regulator (DosR) antigens upgraded during latency and resuscitation-promoting factors (Rpfs) expressed over the reactivation from dormantMycobacterium tuberculosis (M. tuberculosis) could be used to diagnose tuberculosis (TB) at different stages. We performed a retrospective cohort study based on four groups, including healthy controls (HCs), active tuberculosis infections (ATBs), latent tuberculosis infections (LTBIs), and relapse tuberculosis infections (RTBs) enrolled between November 2020 and June 2021. Compared to the fusion protein E6-C10, combined with early secreted antigenic target 6 ...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 25, 2022 Category: Microbiology Source Type: research

True infection or contamination in patients with positive Cutibacterium blood cultures —a retrospective cohort study
AbstractCutibacterium is a genus often considered a contaminant when present in blood cultures, but it can also cause severe infections, especially related to implanted foreign materials. We investigated the incidence and features of patients with trueCutibacterium infection. Patients with positiveCutibacterium blood cultures between the years 2015 –2020 in southern Sweden were identified through microbiology records and medical records were studied retrospectively.Cutibacterium isolates were species determined using MALDI-TOF MS. Patients were classified as having true infection or contamination according to a definitio...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 25, 2022 Category: Microbiology Source Type: research

First report of a prosthetic joint infection with Fannyhessea (Atopobium) vaginae
AbstractThis case describes a 77-year-old woman with dysregulated type II diabetes, presenting with a prosthetic joint infection and bacteremia. Computed tomography (CT) of the pelvis and sacrum revealed manifest periprosthetic collections, suggestive of a septic arthritis with loosening of the hip prosthesis. Synovial fluid grewFannyhessea vaginae, identified by matrix-assisted laser desorption ionization –time of flight mass spectrometry (MALDI-TOF MS). To our knowledge, this is the first report of a prosthetic joint infection due to this organism. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 24, 2022 Category: Microbiology Source Type: research

Antimicrobial activity of ceftazidime-avibactam and comparators against Pseudomonas aeruginosa and Enterobacterales collected in Croatia, Czech Republic, Hungary, Poland, Latvia and Lithuania: ATLAS Surveillance Program, 2019
AbstractAntimicrobial susceptibility of clinical isolates collected from sites in central Europe in 2019 was tested by CLSI broth microdilution method and EUCAST breakpoints. Most active were amikacin, ceftazidime-avibactam and colistin; respectively, susceptibility rates amongP. aeruginosa (n  = 701) were 89.2%, 92.2% and 99.9%; difficult-to-treat (DTR) isolates, 62.5%, 37.5% and 100%; multidrug-resistant (MDR) isolates, 68.3%, 72.9% and 99.5%; meropenem-resistant (MEM-R), metallo-β-lactamase-negative (MBL-negative) isolates, 72.8%, 78.6% and 100%. AmongEnterobacterales (n = 1639), susceptibility to ceftazidime-a...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 20, 2022 Category: Microbiology Source Type: research

Antimicrobial resistance among Haemophilus influenzae isolates responsible for lower respiratory tract infections in Poland, 2005 –2019
AbstractHaemophilus influenzae is a human-specific pathogen responsible for respiratory tract infections, meningitis, and sepsis. The study aimed to characterize antibiotic resistance inH. influenzae strains isolated from patients with lower respiratory tract infections over 15  years in Poland. The minimum inhibitory concentrations (MICs) of clinically relevant antibiotics were determined by broth microdilution method. Screening for beta-lactam resistance was performed in all isolates following EUCAST recommendation. Finally, relevant changes in penicillin-binding prote in 3 (PBP3) were detected by PCR screening. Of the...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 18, 2022 Category: Microbiology Source Type: research

The absolute number of leukocytes per vial as a major cause of early false positive blood cultures: proof-of-concept and application
AbstractBlood cultures detected as positive by the automated system but negative by microscopy and subculture are considered as “false-positives.” Several causes have been identified, including hyperleukocytosis or the presence of fastidious bacteria, but as many cases remain unexplained we aimed to investigate the false positives occurring in our laboratory. We retrospectively collected data on blood cultures received o ver a period of 12 months to determine factors associated with the false-positive vials. We then prospectively validated our findings on the false-positive results occurring over a 3.5-month period. W...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 18, 2022 Category: Microbiology Source Type: research

Contemporary comparison of infective endocarditis caused by Candida albicans and Candida parapsilosis: a cohort study
AbstractAmong 1655 consecutive patients with infective endocarditis treated from 1998 to 2020 in three tertiary care centres, 16 were caused byCandida albicans (CAIE,n = 8) andCandida parapsilosis (CPIE,n = 8). Compared to CAIE, CPIE were more frequently community-acquired. Prosthetic valve involvement was remarkably more common among patients with CPIE. CPIE cases presented a higher rate of positive blood cultures at admission, persistently positive blood cultures after antifungals initiation and positive valve cultures. All patients but four underwent cardiac surgery. Urgent surgery was more frequently performed in CPIE....
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 14, 2022 Category: Microbiology Source Type: research

Retrospective analysis of outcomes of outpatient parenteral antimicrobial therapy (OPAT) for necrotising otitis externa
This study aims to identify factors associated with treatment response and outcome in patients with NOE treated through outpatient parenteral antimicrobial therapy (OPAT). We performed a retrospective analysis of patients with NOE treated over a 4-year period (January 2018 –January 2022) at a tertiary referral hospital in Derbyshire, UK. We defined OPAT failure as unplanned readmission within 30 days of discontinuation of OPAT. Prolonged duration of therapy was defined as length of parenteral antimicrobial treatment of more than 8 weeks. A total of 46 cases of NOE were reviewed. OPAT failure and prolonged therapy were ...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 13, 2022 Category: Microbiology Source Type: research

Disseminated coccidioidomycosis in immunocompetent patients in non-endemic areas: a case series and literature review
This study aimed to review immunocompetent patients diagnosed with disseminated coccidioidomycosis in a tertiary hospital in Eastern China and other non-endemic areas, and to emphasize the importance of combining travel history with clinical manifestations and proper diagnostic examinations. This study retrospectively reviewed a case series of disseminated coccidioidomycosis diagnosed in an academic hospital in Eastern China. We conducted a global literature review of disseminated coccidioidomycosis in immunocompetent patients with travel history. We identified six patients in our case series and reviewed 42 cases in the l...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 12, 2022 Category: Microbiology Source Type: research