Inoculum effect of CTX-M-15, OXA-48, and KPC-2 producing Klebsiella pneumoniae on meropenem and ceftazidime-avibactam efficacy
In this study, 20Klebsiella pneumoniae isolates were tested with different inocula (1 –5 × 105 and 1 –5 × 107 cfu/ml) using broth microdilution methods. The inoculum effect was observed in meropenem against OXA-48 + CTX-M-15- and KPC-2-producing isolates but not with ceftazidime/avibactam. Notably, meropenem exhibited inoculum effect against carbapenemase-producing strains, whereas ceftazidime-avibact am remained effective. We conclude that ceftazidime-avibactam is recommended for high-inoculum infections. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - January 13, 2024 Category: Microbiology Source Type: research

Preliminary reading of antibiogram by microdilution for clinical isolates in urine culture
ConclusionsA preliminary antibiogram reading may be useful in urine cultures to reduce the delay before targeted antibiotherapy, especially againstEnterobacteriaceae and Gram-positive cocci, but not in cases of carbapenemase-producing NFGNB. Further local studies are warranted to evaluate the usefulness of this approach in relation to resistance rates. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - January 12, 2024 Category: Microbiology Source Type: research

A multicenter evaluation of the QIAstat-Dx meningitis-encephalitis syndromic test kit as compared to the conventional diagnostic microbiology workflow
ConclusionThe QS-ME is a relevant addition to the syndromic testing landscape to assist in diagnosing infectious ME. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - January 11, 2024 Category: Microbiology Source Type: research

Population-based genomic surveillance of Pseudomonas aeruginosa causing bloodstream infections in a large Canadian health region
ConclusionAn effective multivalent vaccine consisting of five serotypes (O1, O3, O5, O6, O11) would confer protection to  >  70% of Calgary residents withP. aeruginosa BSIs. This study has provided a unique perspective of the population dynamics over time ofP. aeruginosa STs, clades, and serotypes responsible for BSIs. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - January 10, 2024 Category: Microbiology Source Type: research

Clinical outcomes and complications of S53P4 bioactive glass in chronic osteomyelitis and septic non-unions: a retrospective single-center study
ConclusionS53P4 bioactive glass is an effective and safe therapeutic option in the treatment of chronic osteomyelitis and septic non-unions because of its unique antibacterial properties, but also for its ability to generate a growth response in the remaining healthy bone at the bone-glass interface. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - January 9, 2024 Category: Microbiology Source Type: research

Accuracy of gastric nodule combined with rapid urease test prediction in diagnosing Helicobacter pylori infection in children
ConclusionsThe findings of this study suggest that gastric nodules, particularly when combined with RUT, can be a valuable predictor ofH. pylori infection in children, offering a simple and feasible alternative to other invasive methods. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - January 5, 2024 Category: Microbiology Source Type: research

HPV positivity status in males is related to the acquisition of HPV infection in females in heterosexual couples
ConclusionHusbands ’ HPV positivity increases the burden of non-HR-HPV infection and increases the risk of cervical lesions developing in wives. It is hoped to provide a reference value for cervical cancer prevention in females and HPV vaccination in males. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - January 4, 2024 Category: Microbiology Source Type: research

Mortality risk factors and fulminant sub-phenotype in anaerobic bacteremia: a 10-year retrospective, multicenter, observational cohort study
ConclusionsThis study identified acute circulatory dysfunction and performance of drainage as independent predictive factors for 30-day AB-related mortality and revealed the existence of a fulminant AB sub-phenotype. Our findings could serve as a practical guide to predict the clinical outcomes of AB. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - January 3, 2024 Category: Microbiology Source Type: research

Differences in PPD- and mitogen-induced T-cell activation marker expression characterize immunopathology in acute tuberculosis patients
AbstractImpaired T-cell responses to mitogens and high T-cell activation marker (TAM) expression onMycobacterium tuberculosis–specific T-cells characterize immunopathology in patients with tuberculosis (TB). In a study of patients with TB (n = 60) and asymptomatic contacts (controls,n = 37), we found that TB patients had higher CD38+ T-cell proportions specific forM. tuberculosis protein (PPDMtb), yet total proportions of PPDMtb-specific T-cells were comparable. Notably, both activated (CD38+) and total IFN- γ+ T-cells from TB patients had lower mitogen (phytohemagglutinin, PHA)-induced responses. This impaired mitogen ...
Source: European Journal of Clinical Microbiology and Infectious Diseases - January 3, 2024 Category: Microbiology Source Type: research

Activity of imipenem/relebactam and comparators against KPC-producing Klebsiella pneumoniae and imipenem-resistant Pseudomonas aeruginosa
ConclusionImipenem/relebactam showed excellent activity againstK. pneumoniae KPC-3. The activity of imipenem/relebactam against imipenem-resistantP. aeruginosa was moderate. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - December 29, 2023 Category: Microbiology Source Type: research

The implication of infection with respiratory syncytial virus in pediatric recurrent wheezing and asthma: knowledge expanded post-COVID-19 era
ConclusionIn conclusion, this study offers a comprehensive perspective to better understand how the RSV infection interacts in the control of the host ’s pulmonary immune system, causing recurrent wheezing and the development of asthma, and it sheds fresh light on potential avenues for pharmaceutical therapy in the future. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - December 28, 2023 Category: Microbiology Source Type: research

Semi-quantitative assessment of gastrointestinal viruses in stool samples with Seegene Allplex gastrointestinal panel assays: a solution to the interpretation problem of multiple pathogen detection?
ConclusionBy omitting very high Ct values and reporting semi-quantitatively, value was added to the syndromic reports, leading to an easier to read lab report, especially in mixed infections. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - December 26, 2023 Category: Microbiology Source Type: research

An improved protocol for bacteria identification by MALDI-TOF MS directly from positive blood cultures
AbstractFASTinov ® developed a rapid antimicrobial susceptibility test that includes the purification of a bacterial suspension directly from positive blood cultures (BC). In order to streamline laboratory workflow, the use of the bacterial suspension obtained through FASTinov® sampleprep was tested for identification (ID) by matrix absorption laser deionization –time of flight mass spectrometry (MALDI-TOF MS) (Bruker) in 364 positive BC, and its accuracy assessed comparing with the MALDI-TOF MS ID of the next-day subcultured colonies. FASTinov sample prep  was highly reliable for rapid ID directly from BC with propo...
Source: European Journal of Clinical Microbiology and Infectious Diseases - December 19, 2023 Category: Microbiology Source Type: research

Clinical characteristics and antimicrobial susceptibility of Fusobacterium species isolated over 10  years at a Japanese university hospital
ConclusionWe show a link betweenFusobacterium species and solid organ malignancies. We observed resistance to penicillin, cefmetazole, clindamycin, and moxifloxacin, warranting caution in their clinical use. This study offers valuable insights for managingFusobacterium infections and guiding empirical treatments. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - December 19, 2023 Category: Microbiology Source Type: research

The first report of Apiotrichum mycotoxinivorans isolation from human cerebrospinal fluid
AbstractFungal infections due toApiotrichum mycotoxinivorans are clinically rare. Here, we report a case of invasive blood and cerebrospinal fluid infection byApiotrichum mycotoxinivorans in a girl with B-cell acute lymphoblastic leukemia. This is the first report of the isolation ofApiotrichum mycotoxinivorans from human cerebrospinal fluid. MRI features of meningitis caused by this fungus are presented. Three small isoquinoline alkaloids inhibited the growth of this rare fungusin vitro, providing a starting point for the application of natural products to treat this highly fatal fungal infection. Our case presentation co...
Source: European Journal of Clinical Microbiology and Infectious Diseases - December 16, 2023 Category: Microbiology Source Type: research