European Journal of Clinical Microbiology and Infectious Diseases This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Comparison of chemiluminiscence versus lateral flow assay for the detection of Helicobacter pylori antigen in human fecal samples
In conclusion, theSTANDARD ™ F H. pylori Ag FIA (SD Biosensor) on theSTANDARD ™ F2400 analyser is a highly sensitive, specific and suitable assay for the detection ofH. pylori in stool samples. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 27, 2023 Category: Microbiology Source Type: research

The kinetics of SARS-CoV-2 viremia in COVID-19 patients receiving remdesivir
AbstractDetection of SARS-CoV-2 RNA in serum, viremia, has been linked to disease severity and outcome. The kinetics of viremia in patients receiving remdesivir has not been thoroughly studied and could help predict treatment response and outcome. We investigated the kinetics of SARS-CoV-2 viremia and factors associated with baseline viremia, viral clearance and 30-day mortality in patients receiving remdesivir. An observational study including 378 hospitalised patients (median age 67 years, 67% male) sampled with serum SARS-CoV-2 RT-PCR within ± 24 h of initiation of remdesivir treatment. Baseline viremia was pres...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 27, 2023 Category: Microbiology Source Type: research

Acute otitis externa and auricular erysipelas; causative pathogens, antimicrobial susceptibility and treatment strategies of tertiary referral centre patients
(Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 27, 2023 Category: Microbiology Source Type: research

Pneumococcal vaccination status among cirrhotic patients in Italy: a neglected topic
AbstractTo date, few reports have evaluated the pneumococcal vaccination status in cirrhotic patients. No data are available for European countries. We have explored this topic and the potential independent predictors motivating lack of vaccination in Italy. Between January 1st and June 30th 2022, 1419 cirrhotic patients of any etiology were consecutively enrolled in an observational, prospective study at 8 referral centers in Italy. Adjusted odds ratios (ORs) for the association with lack of vaccination were evaluated by multiple logistic regression analysis. Overall vaccine coverage was 17.9% (8.9% in patients< 65 yea...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 12, 2023 Category: Microbiology Source Type: research

Assessing the quality of the anaerobic environment — a method developed to support EUCAST disk diffusion of anaerobic bacteria
AbstractThe purpose of this study is to establish a method for assessing the anaerobic environment for EUCAST disk diffusion antimicrobial susceptibility testing (AST) of anaerobic bacteria on fastidious anaerobe agar with 5% mechanically defibrinated horse blood (FAA-HB). The method utilizes the association between a decrease in the metronidazole disk zone diameter and increasing oxygen levels with an aerotolerantClostridium perfringens strain DSM 25589 (CCUG 75076 and NCTC 14679). TheC. perfringens strain was tested on FAA-HB with a McFarland 1 inoculum and a metronidazole 5 μg disk. FAA-HB was incubated for 16–20 h a...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 12, 2023 Category: Microbiology Source Type: research

Tick-tock, beat the clock: comparative analysis of disc diffusion testing with 6-, 10-, and 24-h growth for accelerated antimicrobial susceptibility testing and antimicrobial stewardship
AbstractDisc diffusion testing by Kirby-Bauer technique is the most used method for determining antimicrobial susceptibility in microbiological laboratories. The current guidelines by The Clinical and Laboratory Standards Institute (CLSI) 2022 specify using an 18- to 24-h growth for testing by disc diffusion. We aim to determine if using an early growth (6 h and 10 h) would produce comparable results, thus ultimately leading to reduced turnaround time. Six-hour, 10-h, and 24-h growths of 20 quality control strains and 6-h and 24-h growths of 48 clinical samples were used to perform disc diffusion testing using a panel o...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 12, 2023 Category: Microbiology Source Type: research

Epidemiology and antimicrobial resistance profile of Neisseria gonorrhoeae in Catalonia, Spain, 2016 –2019
AbstractAntimicrobial resistance data forNeisseria gonorrhoeae is globally sparse and resistant strains are emerging in Catalonia. We aim to describe epidemiological and antimicrobial resistance in all patients infected withN. gonorrhoeae during the period from 2016 to 2019, using available antimicrobial susceptibility data. We retrospectively analysed confirmed N. gonorrhoeae cases notified to Catalonia ’s microbiological reporting system. Antibiotic susceptibility testing (azithromycin, cefixime, ceftriaxone, ciprofloxacin, penicillin, spectinomycin, and tetracycline) was assessed using clinical breakpoints publish...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 10, 2023 Category: Microbiology Source Type: research

Group B streptococci (GBS) strains evading molecular diagnostics showed novel chromosomal deletions encompassing the CAMP-factor (cfb) encoding gene
AbstractA highly conserved fragment adjacent to thecfb gene encoding the CAMP factor is the target of PCR-based molecular diagnostic systems for the identification ofS. agalactiae (group B streptococci (GBS)). Six PCR-negative, culture-positive GBS strains were whole genome sequenced to assess why they escaped molecular diagnostics. GBS strains did not constitute a clonal cluster and presented variably sized chromosomal deletions (from 7 to 33 kb) which always included thecfb gene, a finding never described before. GBS strains that escape molecular diagnostics are considered rare; however, they can cause false-negative res...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 9, 2023 Category: Microbiology Source Type: research

Profile analysis of emerging respiratory virus in children
The objective of our study was to evaluate clinical data from a viral panel performed in children hospitalized with SARS or COVID-19 in the infirmary or ICU of 5 pediatric hospitals in the city of Goi ânia, Goiás, Brazil. Demographic, clinical, and laboratory data were collected for analysis, and data on the outcomes underwent statistical treatment. A total of 128 patients were selected for the study, 54% of whom were male and 46% female. The viral panel included rhinovirus, COVID-19, metapneum ovirus, adenovirus, and parainfluenza. Descriptive analyses of age profile showed differences in the involvement of particular v...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 9, 2023 Category: Microbiology Source Type: research

Molecular detection of Nocardia: development and application of a real-time PCR assay in sputum and bronchoalveolar lavage fluid samples
In this study, our objective was to develop and validate a new TaqMan real-time PCR (qPCR) assay for rapidly detectingNocardia spp. in respiratory samples. Based on published sequence data, primers in a conserved region of the 16S rRNA gene and a probe within that region that was specific forNocardia were designed. The distinction effect of the qPCR assay was assessed betweenNocardia and other respiratory-associated bacteria. Furthermore, the specificity and sensitivity of the assay were evaluated in respiratory clinical samples (n = 205), compared to the results of 16S rRNA gene amplicon sequencing and clinical diagno...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 9, 2023 Category: Microbiology Source Type: research

The interaction between liver cirrhosis, infection by Streptococcus bovis, and colon cancer
In conclusion, risk of colorectal neoplasm is high among cirrhotic patients withS. gallolyticus bacteremia. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 5, 2023 Category: Microbiology Source Type: research

Clinical epidemiology of pulmonary aspergillosis in hospitalized patients and contribution of Cyp51A, Yap1, and Cdr1B mutations to voriconazole resistance in etiologic Aspergillus species
AbstractPulmonary aspergillosis is a life-threatening fungal infection with worldwide distribution. In the present study, clinical epidemiology of pulmonary aspergillosis and antifungal susceptibility of etiologicAspergillus species were evaluated in one-hundred fifty patients with special focus on the frequency of voriconazole resistance. All the cases were confirmed by the clinical pictures, laboratory findings, and isolation of etiologicAspergillus species which belonged to two major species, i.e.,A. flavus andA. fumigatus. Seventeen isolates displayed voriconazole MIC greater than or equal to the epidemiological cutoff...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 5, 2023 Category: Microbiology Source Type: research