Progressive in vivo development of resistance to cefiderocol in Pseudomonas aeruginosa
We report in vivo development of cefiderocol (FDC) resistance among four sequentialPseudomonas aeruginosa clinical isolates ST244 recovered from a single patient, without exposure to FDC, which raises concern about the effectiveness of this novel drug. The first recoveredP. aeruginosa isolate (P-01) was susceptible to FDC (2  μg/mL), albeit this MIC value was higher than that of a wild-typeP. aeruginosa (0.12 –0.25 μg/ml). The subsequent isolated strains (P-02, P-03, P-04) displayed increasing levels of FDC MICs (8, 16, and 64 μg/ml, respectively). Those isolates also showed variable and gradual increasing levels o...
Source: European Journal of Clinical Microbiology and Infectious Diseases - November 15, 2022 Category: Microbiology Source Type: research

Effect of restricting piperacillin/tazobactam prescription on rates of antimicrobial resistance in gram-negative bacteria and antibiotic consumption
This study aimed to assess the changes in the consumption of antimicrobials and antimicrobial resistance rates after implementing piperacillin/tazobactam restriction. This study was conducted at Kandong Sacred Heart Hospital. We retrospectively collected and analysed data between October 2018 and May 2021 to evaluate antibiotic consumption and resistance patterns after restricting piperacillin/tazobactam. This study included two periods, a 16-month pre-restriction period and a 16-month post-restriction period. During the study period, there was a significant decrease in the consumption of piperacillin/tazobactam after impl...
Source: European Journal of Clinical Microbiology and Infectious Diseases - November 15, 2022 Category: Microbiology Source Type: research

Web-based prediction of antimicrobial resistance in enterococcal clinical isolates by whole-genome sequencing
AbstractBesides phenotypic antimicrobial susceptibility testing (AST), whole genome sequencing (WGS) is a promising alternative approach for detection of resistance phenotypes. The aim of this study was to investigate the concordance between WGS-based resistance prediction and phenotypic AST results for enterococcal clinical isolates using a user-friendly online tools and databases. A total of 172 clinical isolates (34E. faecalis, 138E. faecium) received at the French National Reference Center for enterococci from 2017 to 2020 were included. AST was performed by disc diffusion or MIC determination for 14 antibiotics accord...
Source: European Journal of Clinical Microbiology and Infectious Diseases - November 15, 2022 Category: Microbiology Source Type: research

A previously documented Neisseria macacae isolate providing a false-positive result with Roche cobas 4800 CT/NG does not cross-react with the later generation cobas 6800 CT/NG assay
(Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - November 14, 2022 Category: Microbiology Source Type: research

Successful treatment of ceftazidime/avibactam combined with aztreonam in the NDM-producing Klebsiella pneumoniae bloodstream and intestinal infections in a NK/T lymphoma patient with agranulocytosis during autologous hematopoietic stem cell transplantation: a case report
We described a patient diagnosed with NK/T cell lymphoma who underwent autologous hematopoietic stem cell transplantation (ASCT) in the hematology department. The patient developed severe infection after ASCT. Blood and stool cultures showed carbapenem-resistantK. pneumoniae. Blood sample was detected as NDM-producingK. pneumoniae. We successfully treated this infection with CAZ/AVI and ATM. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - November 12, 2022 Category: Microbiology Source Type: research

The prevalence of Mycoplasma genitalium (MG) and Trichomonas vaginalis (TV) at testing centers in Belgium, Germany, Spain, and the UK using the cobas TV/MG molecular assay
The objective of this study was to use the cobas® TV/MG assay to assess the point prevalence of TV and MG in specimens from men and women over a broad European geographical area. Urine, vaginal, endocervical, and rectal samples were collected from patients aged  ≥ 18 years receivingChlamydia trachomatis (CT) and/orNeisseria gonorrhoeae (NG) screening as per local standard of care at sites in Belgium, Germany, Spain, and the UK (Wales). Remnant samples were assessed using the cobas TV/MG assay. Analysis of 2795 samples showed that MG prevalence varied slightly across female sample types (range: 1.7 –5.8%;p = 0...
Source: European Journal of Clinical Microbiology and Infectious Diseases - November 11, 2022 Category: Microbiology Source Type: research

Risk scores ’ performance and their impact on operative decision-making in left-sided endocarditis: a cohort study
AbstractThe accuracy of contemporary risk scores in predicting perioperative mortality in infective endocarditis (IE) remains controversial. The aim is to evaluate the performance of existent mortality risk scores for cardiovascular surgery in IE and the impact on operability at high-risk thresholds. A single-center retrospective review of adult patients diagnosed with acute left-sided IE undergoing surgery from May 2014 to August 2019 (n = 142) was done. Individualized risk calculation was obtained according to the available mortality risk scores: EuroScore I and II, PALSUSE, Risk-E, Costa, De Feo-Cotrufo, AEPEI, ...
Source: European Journal of Clinical Microbiology and Infectious Diseases - November 8, 2022 Category: Microbiology Source Type: research

Osteomyelitis caused by Pasteurella multocida and Bacteroides pyogenes after cat bite
(Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - November 8, 2022 Category: Microbiology Source Type: research

Surveillance of tick-borne encephalitis in emerging risk areas in southern Sweden: a retrospective case finding study
This study shows a low frequency of TBE sampling among patients with meningoencephalitis in a region with emerging risk for TBE. A higher awareness of TBE as differential diagnosis could contribute to earlier detection of new risk areas and ad equate preventive advice to the public. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - November 2, 2022 Category: Microbiology Source Type: research

Coexistence of tet(A) and blaKPC-2 in the ST11 hypervirulent tigecycline- and carbapenem-resistant Klebsiella pneumoniae isolated from a blood sample
This study aimed to characterize a hypervirulent tigecycline-resistant and carbapenem-resistantKlebsiella pneumoniae strain, XJ-K2, collected from a patient ’s blood. We tested antimicrobial susceptibility, virulence, and whole-genome sequencing (WGS) on strain XJ-K2. WGS data were used to identify virulence and resistance genes and to perform multilocus sequence typing (MLST) and phylogenetic analysis. Three novel plasmids, including a pLVPK-like vir ulence plasmid (pXJ-K2-p1) and two multiple resistance plasmids (pXJ-K2-KPC-2 and pXJ-K2-p3), were discovered in strain XJ-K2. The IncFII(pCRY) plasmid pXJ-K2-p3 carried th...
Source: European Journal of Clinical Microbiology and Infectious Diseases - November 2, 2022 Category: Microbiology Source Type: research

Outcomes and factors contributing to poor prognosis of Pneumocystis jirovecii pneumonia in HIV-negative patients: a cross-sectional retrospective study in a Chinese single center
AbstractPneumocystis jirovecii pneumonia (PJP) is a life-threatening opportunistic infection mainly occurring in immunocompromised patients. Almost half of the 30 HIV-negative patients enrolled in this study from 2016 –2020 in a Chinese single-center contracted 17 hematological malignancies, and 25 received long-term systemic corticosteroids. Only 4 patients received prophylaxis. The overall mortality was 30%. Patients with older age (>  43 years), dyspnea, and LDH >  404U/L had significantly higher risk of developing into a severe form. LDH >  424 U/L, PaO2 <  60 mmHg, monocyte <  0.2...
Source: European Journal of Clinical Microbiology and Infectious Diseases - November 1, 2022 Category: Microbiology Source Type: research

Influenza and other respiratory viruses in children: prevalence and clinical features
AbstractWith the COVID-19 pandemic still ongoing, the annual season of influenza and other respiratory virus epidemics has arrived. Specimens from patients suspected of respiratory viruses infection were collected. Viral detection was performed following RNA extraction and real-time RT-PCR. During the study period, we received and tested a total of 606 specimens. Rhinovirus virus was the viral type most prevalent, detected in 186 (45.47%) specimens. The age range of patients positive for influenza A, influenza A (H1N1), and influenza B was 18  days to 13 years. With female prevalence for this viral type, cough and asthma...
Source: European Journal of Clinical Microbiology and Infectious Diseases - October 26, 2022 Category: Microbiology Source Type: research

Effectiveness of the 23-valent pneumococcal polysaccharide vaccine against invasive pneumococcal disease among 948,263 individuals  ≥ 65 years of age: a Danish cohort study
This study aimed to estimate the effectiveness of the 23-valent pneumococcal polysaccharide vaccine (PPV23) against invasive pneumococcal disease (IPD) among individuals  ≥ 65 years of age. We used Danish nationwide databases to obtain information on PPV23 vaccination, covariates, and IPD and linked data on an individual level using a unique personal identifier. A total of 948,263 individuals were included and followed between June 15, 2020, and September 18, 2021 (58.6% were vaccinated during follow-up). The adjusted vaccine effectiveness was 42% (95% confidence interval (CI): 9–63%) for all-serotype IPD and 58% ...
Source: European Journal of Clinical Microbiology and Infectious Diseases - October 25, 2022 Category: Microbiology Source Type: research

Development and validation of an efficient nomogram for risk assessment of norovirus infection in pediatric patients
This study aimed to establish a predictive model and nomogram based on routine laboratory blood indicators and clinical symptoms, subsequently providing a rapid risk assessment of norovirus (NoV) infection in children. This retrospective study enrolled 307 pediatric patients with symptoms of acute gastroenteritis and detected NoV using real-time quantitative polymerase chain reaction. Significant indicators selected by multivariate logistic regression, including routine blood tests and consultation symptoms, were used to develop the nomogram. We divided the sample into training and internal validation sets and performed ex...
Source: European Journal of Clinical Microbiology and Infectious Diseases - October 25, 2022 Category: Microbiology Source Type: research

Klebsiella pneumoniae ST147: and then there were three carbapenemases
We described the first complete genome sequence of aK. pneumoniae isolate with three different carbapenemases, providing a reference for future studies on this rarely reported occurrence. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - October 22, 2022 Category: Microbiology Source Type: research