European Journal of Clinical Microbiology and Infectious Diseases This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Detection of Trichomonas vaginalis by Allplex ™ STI Essential Assay (Seegene) in clinical samples from the Brazilian public health system users
In this report, urine and vaginal discharge samples were analyzed by wet mount, culture examination, and real-time PCR by Allplex ™ (Seegene®) kit, in a population assisted by the Brazilian Public Health System. From 747 samples, 2.81% were positive for TV in wet mount and culture, and 3.88% by Allplex™. Samples kept at − 80 ºC for 22 months did not impair the PCR technique. The sensitivity for wet mount, culture, and Allplex™ was 72, 100, and 100%, respectively. Allplex™ technique showed highest detection of TV. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - November 2, 2023 Category: Microbiology Source Type: research

Rapid detection of cefiderocol susceptibility/resistance in Acinetobacter baumannii
ConclusionsThe rapid FDCAcinetobacter baumannii NP test can be a valuable method which is easier and faster to interpret when compared with the gold standard broth microdilution method. The test showed remarkable performances; hence, it may be suitable for implementation in clinical microbiology routine laboratories. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - November 1, 2023 Category: Microbiology Source Type: research

Impact of posaconazole prophylaxis and antifungal treatment on BAL GM performance in hematology malignancy patients with febrile neutropenia: a real life experience
ConclusionOur study shows that posaconazole prophylaxis and active short-term (3  days) antifungal treatment do not significantly affect overall diagnostic performance of GM measurements in bronchoalveolar lavage and bronchial lavage samples. However, using different cut-off values for patients receiving active treatment might be suggested to increase sensitivity. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - November 1, 2023 Category: Microbiology Source Type: research

Amino acid substitutions in the region between RpoB clusters II and III on rifampin susceptibility in Haemophilus influenzae
ConclusionsOur findings indicate that L672F substitution in the region between RpoB clusters II and III has an aggravating effect on rifampin resistance inH. influenzae. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - October 31, 2023 Category: Microbiology Source Type: research

Co-regulation of biofilm formation and antimicrobial resistance in Acinetobacter baumannii: from mechanisms to therapeutic strategies
AbstractIn recent years, multidrug-resistantAcinetobacter baumannii has emerged globally as a major threat to the healthcare system. It is now listed by the World Health Organization as a priority one for the need of new therapeutic agents.A.baumannii has the capacity to develop robust biofilms on biotic and abiotic surfaces. Biofilm development allows these bacteria to resist various environmental stressors, including antibiotics and lack of nutrients or water, which in turn allows the persistence ofA.baumannii in the hospital environment and further outbreaks. Investigation into therapeutic alternatives that will act on ...
Source: European Journal of Clinical Microbiology and Infectious Diseases - October 28, 2023 Category: Microbiology Source Type: research

Production of OXA-48 carbapenemase acts as an independent risk factor for poor outcome in Klebsiella pneumoniae infection
ConclusionOur matched analysis suggests that the production of OXA-48 carbapenemase acts as an independent risk factor for poor outcome inK. pneumoniae infection as compared to episodes due to non-carbapenemase-producing strains. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - October 27, 2023 Category: Microbiology Source Type: research

Increased recognition of Q fever aortitis as a chronic manifestation of Q fever in tropical North Queensland, Australia
We report a series of 5 patients with Q fever aortitis who have presented to our hospital in tropical Australia since 2019. All diagnoses were confirmed with polymerase chain reaction (PCR) testing of aortic tissue. Only one had a previous diagnosis of acute Q fever, and none had classical high-risk exposures that might increase clinical suspicion for the infection. All patients underwent surgery: one died and 3 had significant complications. Q fever aortitis may be underdiagnosed; clinicians should consider testing forCoxiella burnetii in people with aortic pathology in endemic areas. (Source: European Journal of Clinical...
Source: European Journal of Clinical Microbiology and Infectious Diseases - October 26, 2023 Category: Microbiology Source Type: research

Multicenter interlaboratory study of routine systems for the susceptibility testing of temocillin using a challenge panel of multidrug-resistant strains
AbstractAccurate susceptibility result of temocillin (TMO) is important for treating infections caused by multidrug-resistantEnterobacterales. This multicenter study aimed to investigate the performance of routine temocillin testing assays againstEnterobacterales challenging strains.  Forty-seven selected clinical isolates were blindly analyzed by 12 Belgian laboratories using VITEK® 2 (n = 5) and BD Phoenix™ (n = 3) automated systems, ETEST® gradient strip (n = 3), and disk (3 brands) diffusion method (DD;n = 6) for temocillin susceptibility using standardized methodology. Results were interpreted usi...
Source: European Journal of Clinical Microbiology and Infectious Diseases - October 23, 2023 Category: Microbiology Source Type: research

Core genome multilocus sequence typing of Clostridioides difficile to investigate transmission in the hospital setting
ConclusionAmong clusters determined by cgMLST analysis, roughly 40% included unexplained HA-CDI acquisitions, which may be explained by unidentified epidemiological links, asymptomatic colonization, and/or shared common community reservoirs. The use of DLST, followed by whole genome sequencing analysis, is a promising and cost-effective stepwise approach for the investigation of CDI transmission in the hospital setting. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - October 23, 2023 Category: Microbiology Source Type: research

Severe acute herpes virus type 2 primo-infection and its association with anti-type 1 interferon autoantibodies
AbstractHerpes simplex virus type 2 (HSV-2) is a common cause of infection, which is usually self-limited and asymptomatic. A 71-year-old patient with HSV-2 primo-infection developed acute hepatitis and secondary hemophagocytic lymphohistiocytosis. The patient had high levels of autoantibodies against type I interferon (IFN) (>  1000 ng/mL), neutralizing high concentration (10 ng/mL) of both IFN-α and IFN-ω but not IFN-β. Anti-IFN-I auto-antibodies are rarely observed in healthy individuals; however, their prevalence increases in individuals over 70 years of age and have been identified as a cause of some severe vi...
Source: European Journal of Clinical Microbiology and Infectious Diseases - October 23, 2023 Category: Microbiology Source Type: research

Persistent headaches one year after bacterial meningitis: prevalence, determinants and impact on quality of life
ConclusionPersistent headaches are frequent one year after meningitis and are associated with quality of life alteration.Clinical trialNCT01730690. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - October 23, 2023 Category: Microbiology Source Type: research

Late HBV reactivation after hematopoietic stem cell transplantation, despite long-term prophylaxis
(Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - October 23, 2023 Category: Microbiology Source Type: research

High risk of intestinal colonization with ESBL-producing Escherichia coli among soldiers of military contingents in specific geographic regions
AbstractOne-hundred Polish soldiers of a contingent in Afghanistan in 2019 were screened forEnterobacterales resistant to newer-generationβ-lactams at their departure and return. Seventeen percent were colonized in the gut at the departure, whereas 70% acquired carriage in Afghanistan. The commonest organisms were extended-spectrumβ-lactamase (ESBL)-producingEscherichia coli (ESBL-Ec; 96.6%). All isolates were sequenced and were clonally diverse overall, even within the same sequence type, indicating that independent acquisitions mainly. ESBL-Ec were often multi-drug-resistant. Soldiers stationing in certain regions are ...
Source: European Journal of Clinical Microbiology and Infectious Diseases - October 19, 2023 Category: Microbiology Source Type: research

Effectiveness of a multimodal intervention to improve blood culture collection in an adult emergency department
AbstractWe investigated the impact of a multimodal intervention to improve the compliance of BC collections as a composite outcome, taking into account both blood volume collected and absence of solitary BC. We performed a quasi-experimental study using a before-after design (5  months for pre- and post-intervention evaluation) in an adult emergency department at a tertiary care hospital that showed that a multimodal intervention was associated with a dramatic increase in the proportion of blood cultures that were collected as recommended per national guidelines, from 17. 3% (328/1896) to 68.9% (744/1080),P <  0.00...
Source: European Journal of Clinical Microbiology and Infectious Diseases - October 19, 2023 Category: Microbiology Source Type: research

Country-wide expansion of a VIM-1 carbapenemase-producing Klebsiella oxytoca ST145 lineage in Poland, 2009 –2019
ConclusionKoSC has been one of the major VIM producers in Poland, owing largely to clonal expansion of the specificK. oxytoca–In237-like lineage. Its apparently enhanced epidemic potential may create a danger on international scale. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - October 19, 2023 Category: Microbiology Source Type: research