Genomic characterization of emerging invasive Streptococcus agalactiae serotype VIII in Alberta, Canada
Abstract Invasive Group B Streptococcus (GBS) can infect pregnant women, neonates, and older adults. Invasive GBS serotype VIII is infrequent in Alberta; however, cases have increased in recent years. Here, genomic analysis was used to characterize fourteen adult invasive serotype VIII isolates from 2009 to 2021. Trends in descriptive clinical data and antimicrobial susceptibility results were evaluated for invasive serotype VIII isolates from Alberta. Isolate genomes were sequenced and subjected to molecular sequence typing, virulence and antimicrobial resistance gene identification, phylogenetic analysis, and pangenom...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 21, 2023 Category: Microbiology Source Type: research

Development of subfamily-based consensus PCR assays for the detection of human and animal herpesviruses
AbstractConsensus PCR assays that can be used to sensitively detect several herpesvirus (HV) species across the different subfamilies were developed in this study. Primers containing degenerate bases were designed to amplify regions of the DNA polymerase (DPOL) gene of alpha- and gamma-HVs, and the glycoprotein B (gB) gene of beta-HVs in a singleplex, non-nested touchdown PCR format. The singleplex touchdown consensus PCR (STC-PCR) was used to amplify the DNA of eight human and 24 animal HVs. The assay was able to detect the lowest DNA dilution of 10−5 for alpha-HVs and 10−3 for beta- and gamma-HVs. In comparison, lowe...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 21, 2023 Category: Microbiology Source Type: research

Risk of hepatitis B reactivation following baricitinib or tocilizumab for treatment of COVID-19
(Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 21, 2023 Category: Microbiology Source Type: research

Undiagnosed and missed active pulmonary tuberculosis during mass gatherings: a prospective cross-sectional study from the Hajj pilgrimage
AbstractMass gatherings increase the risk of infectious diseases transmission including tuberculosis (TB). The Hajj pilgrimage to Mecca, Saudi Arabia, is attended by over 2 million pilgrims many of whom are from high TB-burden countries, and has been linked to increased risk of TB acquisition among travellers. We investigated the burden of undiagnosed and missed active pulmonary TB (PTB) among Hajj pilgrims symptomatic for cough. The study was conducted among hospitalised and non-hospitalised travellers attending the Hajj pilgrimage in 2016 and 2017. Questionnaires were used to collect relevant data and sputum samples were...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 19, 2023 Category: Microbiology Source Type: research

Author ’s reply
(Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 18, 2023 Category: Microbiology Source Type: research

Antimicrobial use, healthcare-associated infections, and bacterial resistance in general hospitals in China: the first national pilot point prevalence survey report
AbstractThe purpose of this study is to implement point prevalence survey (PPS), assess antimicrobial prescribing and resistance in general hospitals and clinical specialties in China, and compare them with similar data from other parts of the world. Twenty general hospitals in China were surveyed in October or November, 2019. A standardized surveillance protocol was used to collect data on patient demographics, diagnosis of infection, the prevalence and intensity of antimicrobial use, prescribing quality, bacterium type and resistance spectrum, and the prevalence and type of healthcare-associated infections (HAIs). Overal...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 17, 2023 Category: Microbiology Source Type: research

A rare case of presumptive pleural toxocariasis
We describe a case of pleurisy with isolated eosinophilia varying between 600 and 1500/mm3 likely linked o presumptive toxocariasis in a 72-year-old patient. Our patient was admitted to hospital with severe dyspnoea, asthenia and diarrhoea. Imaging studies confirmed right unilateral pleurisy without any parenchymal involvement. Serology of serum and pleural fluid was positive for anti-Toxocara antibodies by ELISA and immunoblotting. Treatment by pleural drainage and anti-parasitic medication with albendazole for 8  days resulted in the resolution of symptoms. A decrease in the levels of polynuclear eosinophils and total I...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 15, 2023 Category: Microbiology Source Type: research

A comparison of five Illumina, Ion Torrent, and nanopore sequencing technology-based approaches for whole genome sequencing of SARS-CoV-2
In conclusion, the studied protocols differed on a variety of the studied metrics. This study provides data that assist laboratories when selecting protocols for their specific setting. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 5, 2023 Category: Microbiology Source Type: research

Evaluation of a sterile, filter-based, in-house method for rapid direct bacterial identification and antimicrobial susceptibility testing using positive blood culture
This study aimed to assess the performance of our in-house method for rapid direct bacterial identification (ID) and antimicrobial susceptibility testing (AST) using a positive blood culture (BC) broth. For Gram-negative bacteria, 4  mL of BC broth was aspirated and passed through a Sartorius Minisart syringe filter with a pore size of 5 µm. The filtrate was then centrifuged and washed. A small volume of the pellet was used for ID, using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and for AS T, using automated broth microdilution. For Gram-positive cocci, 4 mL of BC broth was passed th...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 3, 2023 Category: Microbiology Source Type: research

Clinical usefulness of metagenomic next-generation sequencing for Rickettsia and Coxiella burnetii diagnosis
AbstractRickettsia andCoxiella burnetii are zoonotic tick-borne pathogens that cause febrile illnesses in humans. Metagenomic next-generation sequencing (mNGS) is a new technology used to diagnose infectious diseases. However, clinical experience with applying the test to rickettsioses and Q fever is relatively limited. Therefore, this study aimed to explore the diagnostic performance of mNGS in detectingRickettsia andC. burnetii.  We retrospectively studied patients with rickettsioses or Q fever between August 2021 and July 2022. Peripheral blood mNGS and polymerase chain reaction (PCR) were performed for all patients. C...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 30, 2023 Category: Microbiology Source Type: research

Two-site study on performances of a commercially available MALDI-TOF MS-based assay for the detection of colistin resistance in Escherichia coli
AbstractColistin is a last resort drug for the treatment of multiple drug-resistant (MDR) Gram-negative bacterial infections. Rapid methods to detect resistance are highly desirable. Here, we evaluated the performance of a commercially available MALDI-TOF MS-based assay for colistin resistance testing inEscherichia coli at two different sites. Ninety clinicalE. coli isolates were provided by France and tested in Germany and UK using a MALDI-TOF MS-based colistin resistance assay. Lipid A molecules of the bacterial cell membrane were extracted using the MBT Lipid Xtract Kit ™ (RUO; Bruker Daltonics, Germany). Spectra acqu...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 27, 2023 Category: Microbiology Source Type: research

Carriage of multidrug-resistant Gram-negative bacilli: duration and risk factors
AbstractIdentification of risk factors influencing the duration of carriage of multidrug-resistant Gram-negative bacilli (MDR-GNB) may be useful for infection control. The aim of this study is to estimate the impact of several factors collected for routine hospital surveillance on the duration of carriage of selected MDR-GNB. From January 2015 to July 2021, patients with at least two clinical/surveillance samples positive for MDR-GNB different from ESBL-producingE. coli or AmpC  − exclusively producing Enterobacterales were assessed. Microorganisms, age, number of admissions, clinical or rectal sample, sex, and admis...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 25, 2023 Category: Microbiology Source Type: research

Staphylococcus aureus nasal colonization level and intracellular reservoir: a prospective cohort study
In conclusion, the nasal cavity was also found to be a major site ofS. aureus carriage in humans and should draw attention when studying host-pathogen interactions related to the risk of infection associated with colonization. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 24, 2023 Category: Microbiology Source Type: research

Streptococcus gallolyticus meningitis and Strongyloides stercoralis hyperinfection in a patient with systemic lupus erythematosus
(Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 23, 2023 Category: Microbiology Source Type: research

Genomic epidemiology and transmission dynamics of recurrent Clostridioides difficile infection in Western Australia
AbstractRecurrent cases ofClostridioides difficile infection (rCDI) remain one of the most common and serious challenges faced in the management of CDI. The accurate distinction between a relapse (caused by infection with the same strain) and reinfection (caused by a new strain) has implications for infection control and prevention, and patient therapy. Here, we used whole-genome sequencing to investigate the epidemiology of 94C. difficile isolates from 38 patients with rCDI in Western Australia. TheC. difficile strain population comprised 13 sequence types (STs) led by ST2 (PCR ribotype (RT) 014, 36.2%), ST8 (RT002, 19.1%...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 20, 2023 Category: Microbiology Source Type: research