European Journal of Clinical Microbiology and Infectious Diseases 
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(Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 18, 2023 Category: Microbiology Source Type: research
Antimicrobial use, healthcare-associated infections, and bacterial resistance in general hospitals in China: the first national pilot point prevalence survey report
AbstractThe purpose of this study is to implement point prevalence survey (PPS), assess antimicrobial prescribing and resistance in general hospitals and clinical specialties in China, and compare them with similar data from other parts of the world. Twenty general hospitals in China were surveyed in October or November, 2019. A standardized surveillance protocol was used to collect data on patient demographics, diagnosis of infection, the prevalence and intensity of antimicrobial use, prescribing quality, bacterium type and resistance spectrum, and the prevalence and type of healthcare-associated infections (HAIs). Overal...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 17, 2023 Category: Microbiology Source Type: research
A rare case of presumptive pleural toxocariasis
We describe a case of pleurisy with isolated eosinophilia varying between 600 and 1500/mm3 likely linked o presumptive toxocariasis in a 72-year-old patient. Our patient was admitted to hospital with severe dyspnoea, asthenia and diarrhoea. Imaging studies confirmed right unilateral pleurisy without any parenchymal involvement. Serology of serum and pleural fluid was positive for anti-Toxocara antibodies by ELISA and immunoblotting. Treatment by pleural drainage and anti-parasitic medication with albendazole for 8 days resulted in the resolution of symptoms. A decrease in the levels of polynuclear eosinophils and total I...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 15, 2023 Category: Microbiology Source Type: research
A comparison of five Illumina, Ion Torrent, and nanopore sequencing technology-based approaches for whole genome sequencing of SARS-CoV-2
In conclusion, the studied protocols differed on a variety of the studied metrics. This study provides data that assist laboratories when selecting protocols for their specific setting. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 5, 2023 Category: Microbiology Source Type: research
Evaluation of a sterile, filter-based, in-house method for rapid direct bacterial identification and antimicrobial susceptibility testing using positive blood culture
This study aimed to assess the performance of our in-house method for rapid direct bacterial identification (ID) and antimicrobial susceptibility testing (AST) using a positive blood culture (BC) broth. For Gram-negative bacteria, 4 mL of BC broth was aspirated and passed through a Sartorius Minisart syringe filter with a pore size of 5 µm. The filtrate was then centrifuged and washed. A small volume of the pellet was used for ID, using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and for AS T, using automated broth microdilution. For Gram-positive cocci, 4 mL of BC broth was passed th...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 3, 2023 Category: Microbiology Source Type: research
Clinical usefulness of metagenomic next-generation sequencing for Rickettsia and Coxiella burnetii diagnosis
AbstractRickettsia andCoxiella burnetii are zoonotic tick-borne pathogens that cause febrile illnesses in humans. Metagenomic next-generation sequencing (mNGS) is a new technology used to diagnose infectious diseases. However, clinical experience with applying the test to rickettsioses and Q fever is relatively limited. Therefore, this study aimed to explore the diagnostic performance of mNGS in detectingRickettsia andC. burnetii. We retrospectively studied patients with rickettsioses or Q fever between August 2021 and July 2022. Peripheral blood mNGS and polymerase chain reaction (PCR) were performed for all patients. C...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 30, 2023 Category: Microbiology Source Type: research
Two-site study on performances of a commercially available MALDI-TOF MS-based assay for the detection of colistin resistance in Escherichia coli
AbstractColistin is a last resort drug for the treatment of multiple drug-resistant (MDR) Gram-negative bacterial infections. Rapid methods to detect resistance are highly desirable. Here, we evaluated the performance of a commercially available MALDI-TOF MS-based assay for colistin resistance testing inEscherichia coli at two different sites. Ninety clinicalE. coli isolates were provided by France and tested in Germany and UK using a MALDI-TOF MS-based colistin resistance assay. Lipid A molecules of the bacterial cell membrane were extracted using the MBT Lipid Xtract Kit ™ (RUO; Bruker Daltonics, Germany). Spectra acqu...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 27, 2023 Category: Microbiology Source Type: research
Carriage of multidrug-resistant Gram-negative bacilli: duration and risk factors
AbstractIdentification of risk factors influencing the duration of carriage of multidrug-resistant Gram-negative bacilli (MDR-GNB) may be useful for infection control. The aim of this study is to estimate the impact of several factors collected for routine hospital surveillance on the duration of carriage of selected MDR-GNB. From January 2015 to July 2021, patients with at least two clinical/surveillance samples positive for MDR-GNB different from ESBL-producingE. coli or AmpC − exclusively producing Enterobacterales were assessed. Microorganisms, age, number of admissions, clinical or rectal sample, sex, and admis...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 25, 2023 Category: Microbiology Source Type: research
Staphylococcus aureus nasal colonization level and intracellular reservoir: a prospective cohort study
In conclusion, the nasal cavity was also found to be a major site ofS. aureus carriage in humans and should draw attention when studying host-pathogen interactions related to the risk of infection associated with colonization. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 24, 2023 Category: Microbiology Source Type: research
Streptococcus gallolyticus meningitis and Strongyloides stercoralis hyperinfection in a patient with systemic lupus erythematosus
(Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 23, 2023 Category: Microbiology Source Type: research
Genomic epidemiology and transmission dynamics of recurrent Clostridioides difficile infection in Western Australia
AbstractRecurrent cases ofClostridioides difficile infection (rCDI) remain one of the most common and serious challenges faced in the management of CDI. The accurate distinction between a relapse (caused by infection with the same strain) and reinfection (caused by a new strain) has implications for infection control and prevention, and patient therapy. Here, we used whole-genome sequencing to investigate the epidemiology of 94C. difficile isolates from 38 patients with rCDI in Western Australia. TheC. difficile strain population comprised 13 sequence types (STs) led by ST2 (PCR ribotype (RT) 014, 36.2%), ST8 (RT002, 19.1%...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 20, 2023 Category: Microbiology Source Type: research
Outbreak of OXA-48-producing Enterobacteriaceae in a neonatal intensive care unit in Western Sweden
AbstractIn 2015, an outbreak caused by OXA-48-producingEnterobacteriaceae affected a neonatal intensive care unit at a Swedish University Hospital. The aim was to explore the transmission of OXA-48-producing strains between infants and the transfer of resistance plasmids between strains during the outbreak. Twenty-four outbreak isolates from ten suspected cases were whole-genome sequenced. A complete assembly was created for the index isolate (Enterobacter cloacae) and used as a mapping reference to detect its plasmids in the remaining isolates (17Klebsiella pneumoniae, 4Klebsiella aerogenes, and 2Escherichia coli). Strain...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 20, 2023 Category: Microbiology Source Type: research
Epidemiology and clinical presentation of community-acquired Staphylococcus aureus bacteraemia in children under 5 years of age admitted to the Manhiça District Hospital, Mozambique, 2001–2019
AbstractStaphylococcus aureus bacteraemia (SAB) is one of the most common bloodstream infections globally. Data on the burden and epidemiology of community-acquired SAB in low-income countries are scarce but needed to define preventive and management strategies. Blood samples were collected from children < 5 years of age with fever or severe disease admitted to the Manhiça District Hospital for bacterial isolation, includingS. aureus. Between 2001 and 2019, 7.6% (3,197/41,891) of children had bacteraemia, of which 12.3% corresponded to SAB. The overall incidence of SAB was 56.1 episodes/100,000 children-years at...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 18, 2023 Category: Microbiology Source Type: research
A fatal case of Strongyloides hyperinfection with obscure risk factors
(Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 17, 2023 Category: Microbiology Source Type: research
RIDA ®GENE Helicobacter pylori PCR on the ELITe InGenius System
AbstractPCR detection ofHelicobacter pylori infection in gastric biopsies allows the detection of this bacterium and the mutations associated with macrolide resistance. The aim of this study was to evaluate the performance of RIDA ®GENEH. pylori PCR (r-Biopharm) on the ELITe InGenius System (Elitech). Two hundred gastric biopsies were obtained. These biopsies were ground in nutrient broth. Two hundred microliters of this suspension was treated with proteinase K, and then, 200 µL was transferred to an ELITe InGenius sample tube and tested using RIDA®GENEH. pylori PCR reagents. In-houseH. pylori PCR was used as a referenc...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 16, 2023 Category: Microbiology Source Type: research
