Staphylococcus massiliensis isolated from human blood cultures, Germany, 2017 –2020
We report the discrepancy of microbiological findings between matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, 16S-rRNA polymerase chain reaction, and whole-genome sequencing for all three isolates. Our findings highlight the diagnostic pitfalls pertinent to novel and non-model organisms in daily microbiological practice, in whom the correct identification is dependent on database accuracy. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 21, 2022 Category: Microbiology Source Type: research

Resolving taxonomic confusion: establishing the genus Phytobacter on the list of clinically relevant Enterobacteriaceae
AbstractAlthough many clinically significant strains belonging to the familyEnterobacteriaceae fall into a restricted number of genera and species, there is still a substantial number of isolates that elude this classification and for which proper identification remains challenging. With the current improvements in the field of genomics, it is not only possible to generate high-quality data to accurately identify individual nosocomial isolates at the species level and understand their pathogenic potential but also to analyse retrospectively the genome sequence databases to identify past recurrences of a specific organism, ...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 21, 2022 Category: Microbiology Source Type: research

Mortality-associated factors of candidemia: a multi-center prospective cohort in Turkey
This study aimed to evaluate the risk factors associated with 30-day mortality in patients with candidemia. A multi-center prospective observational study was conducted in seven university hospitals in six provinces in the western part of Turkey. Patient data were collected with a structured form between January 2018 and April 2019. In total, 425 episodes of candidemia were observed during the study period. Two hundred forty-one patients died within 30  days, and the 30-day crude mortality rate was 56.7%. Multivariable analysis found that SOFA score (OR: 1.28, CI: 1.154–1.420,p <  0.001), parenteral nutrition (OR...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 21, 2022 Category: Microbiology Source Type: research

Protocatechuic acid protects mice from influenza A virus infection
AbstractInfluenza A virus (IAV) H1N1 infection remains great challenge to public health and causes great burden over the world. Although there are anti-viral agents available, searching for effective agents to treat H1N1 infection is still in urgent because of the emergence of resistant strain. Protocatechuic acid (PCA) is a biological agent with multiple functions. In present study, we explored the effects of PCA on H1N1 infection. Mice infected with mouse adapted influenza strain A/Font Monmouth were administrated with PCA. The body weight change, mortality, lung index, viral titer, immune cell infiltration, and cytokine...
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 21, 2022 Category: Microbiology Source Type: research

Assessing the diagnostic accuracy of the Xpert MTB/RIF assay in detecting epididymal tuberculosis
ConclusionsWe recommend the Xpert MTB/RIF assay as a diagnostic method for the detection of epididymal TB. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - March 21, 2022 Category: Microbiology Source Type: research