Evaluating Fourier-transform infrared spectroscopy with IR Biotyper as a faster and simpler method for investigating the sources of an outbreak of legionellosis
AbstractFourier-transform infrared (FTIR) spectroscopy using the IR Biotyper and core genome single  nucleotide polymorphism (cgSNP) analysis were performed on 12Legionella isolates associated with an outbreak at a spa house in Kanagawa Prefecture, Japan, and 3 non-outbreak isolates. The discriminative power of FTIR spectroscopy for 48-h incubation conditions ofL.pneumophila in this outbreak was lower than cgSNP-based typing but higher than serogroup typing. FTIR spectroscopy could screen outbreak isolates from a group of genetically related isolates and may be useful as an initial typing method inLegionella outbreak inve...
Source: European Journal of Clinical Microbiology and Infectious Diseases - February 20, 2024 Category: Microbiology Source Type: research

Real-world experience with meropenem/vaborbactam for the treatment of infections caused by ESBL-producing Enterobacterales and carbapenem-resistant Klebsiella pneumoniae
ConclusionsM/V is a promising therapeutic option against infections caused by difficult-to-treat ESBL-producing Enterobacterales and CR-Kp. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - February 20, 2024 Category: Microbiology Source Type: research

Recurrent bacteremia and endocarditis due to Staphylococcus capitis in a patient with Bowen ’s disease: a case report
AbstractThis case report details the management of a 79-year-old male with recurrent methicillin-resistantStaphylococcus capitis bacteremia and endocarditis. The patient ’s clinical journey encompassed multiple hospital admissions, with challenges in managing endocarditis, pacemaker replacements, and potential cutaneous sources of infection. The treatment regimen included intravenous antibiotic therapy during hospitalization and suppressive antibiotic treatment up on discharge, alongside a decolonization strategy for his scalp lesions. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - February 20, 2024 Category: Microbiology Source Type: research

Disseminated nocardiosis and anti-GM-CSF antibodies
We report an apparently immunocompetent woman presenting with disseminated nocardiosis without any evidence of PAP. Clinical data and radiological images were retrospectively collected. Lymphocyte populations were analyzed by flow cytometry. Anti-GM-CSF autoantibodies were measured by ELISA. A 55-year-old otherwise healthy woman presented with cerebral and pulmonary abscesses. Personal and familial history of infections or autoimmunity were negative. After extensive examinations, a final diagnosis of disseminated nocardiosis was made. Immunologic investigations including neutrophilic function and IFN- γ/IL-12 circuitry fa...
Source: European Journal of Clinical Microbiology and Infectious Diseases - February 20, 2024 Category: Microbiology Source Type: research

Risk factors and outcome associated with the acquisition of MDR linezolid-resistant Enterococcus faecium: a report from tertiary care centre
ConclusionThis study provided insight into epidemiology of MDR LREfm in a setting where linezolid use is high. The main drivers of infections with LREfm are multiple, including use of carbapenems and linezolid. Invasive procedures and increased hospital stay facilitate spread through breach in infection control practises. As therapeutic options are limited, ongoing surveillance of LREfm and VRE is critical to guide appropriate use of linezolid and infection control policies. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - February 19, 2024 Category: Microbiology Source Type: research

Global burden of MDR-TB and XDR-TB attributable to high fasting plasma glucose from 1990 to 2019: a retrospective analysis based on the global burden of disease study 2019
ConclusionThe burden of HFPG-attributable MDR-TB and XDR-TB rose worldwide from 1990 to 2019. These findings emphasize the importance of routine bi-directional screening and integrated management for drug-resistant TB and diabetes. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - February 17, 2024 Category: Microbiology Source Type: research

Prolonged viral pneumonia and high mortality in COVID-19 patients on anti-CD20 monoclonal antibody therapy
ConclusionAnti-CD20 monoclonal antibody therapy is associated with high COVID-19 mortality and with a phenotype consistent with prolonged viral pneumonia. Our study provides rationale for retesting of immunocompromised patients with prolonged COVID-19 symptoms and considering antiviral therapy. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - February 15, 2024 Category: Microbiology Source Type: research

Efficient detection of Streptococcus pyogenes based on recombinase polymerase amplification and lateral flow strip
ConclusionsThe utilization of RPA and LFS allows for efficient and rapid testing of GAS, thereby serving as a valuable method for point-of-care testing. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - February 15, 2024 Category: Microbiology Source Type: research

Characterization of an outbreak caused by Elizabethkingia miricola using Fourier-transform infrared (FTIR) spectroscopy
AbstractFourier-transform infrared (FTIR) spectroscopy has the potential to be used for bacterial typing and outbreak characterization. We evaluated FTIR for the characterization of an outbreak caused byElizabethkingia miricola. During the 2020 –2021 period, 26 isolates (23 clinical and 3 environmental) were collected and analyzed by FTIR (IR Biotyper) and core-genome MLST (cgMLST), in addition to antimicrobial susceptibility testing. FTIR spectroscopy and cgMLST showed that 22 of the isolates were related to the outbreak, including the environmental samples, with only one discordance between both methods. Then, FTIR is ...
Source: European Journal of Clinical Microbiology and Infectious Diseases - February 14, 2024 Category: Microbiology Source Type: research

Risk factors for short-term prognosis of end-stage liver disease complicated by invasive pulmonary aspergillosis
ConclusionsGlucocorticoid exposure, invasive procedures, and broad-spectrum antibiotic exposure increased the risk of IPA in ESLD patients. AmB alone or combined with other antifungals may serve as an economical, safe, and effective treatment option for ESLD-IPA. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - February 12, 2024 Category: Microbiology Source Type: research

Rare serotype c Haemophilus influenzae invasive isolate: characterization of the first case in Portugal
We report for the first time in Portugal a serotype cHaemophilus influenzae isolated from an adult, with HIV-1 infection. Whole-genome sequencing characterized the isolate as clonal complex ST-7, albeit with a novel MLST (ST2754) due to a uniqueatpG profile. Integration of this genome with other availableH. influenzae serotype c genomes from PubMLST revealed its overall genetic distinctiveness, with the closest related isolate being identified in France in 2020. This surveillance study, involving collaboration among hospitals and reference laboratory, successfully contributed to the identification and characterization of t...
Source: European Journal of Clinical Microbiology and Infectious Diseases - February 9, 2024 Category: Microbiology Source Type: research

Heterogeneity in practices to reduce the risk of transmission of Clostridioides difficile in healthcare settings: a survey of ESCMID Study Group for Clostridioides difficile (ESGCD) members
AbstractClostridioides difficile is a leading cause of healthcare-associated infections. The main objective was to assess the current landscape of CDI infection prevention and control (IPC) practices. An anonymous survey of IPC practices for CDI was conducted between July 25 and October 31, 2022. Precautions for symptomatic patients were applicable for 75.9% and were discontinued 48  h minimum after the resolution of diarrhea for 40.7% of respondents. Daily cleaning of CDI patients’ rooms was reported by 23 (42.6%). There was unexpected heterogeneity in IPC practices regarding the hospital management of CDI. (Source: Eu...
Source: European Journal of Clinical Microbiology and Infectious Diseases - February 9, 2024 Category: Microbiology Source Type: research

Impact of an interventional bundle on complications associated with peripheral venous catheters in elderly patients
ConclusionsWe found no statistically significant differences regarding phlebitis and catheter tip colonization rates. It is necessary to carry out randomized studies assessing the most cost-effective measure to reduce complications associated with PVC. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - February 8, 2024 Category: Microbiology Source Type: research

Usutu virus avian and human infection after more than 25  years of circulation
(Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - February 8, 2024 Category: Microbiology Source Type: research

Comparison of influenza- and COVID-19-associated pulmonary aspergillosis in China
ConclusionPatients infected with SARS-CoV-2 andAspergillus during the concentrated outbreak of COVID-19 in China had generally higher in-hospital mortality but a lower percentage of chronic pulmonary disease than those infected with influenza andAspergillus. For influenza-infected patients who require hospitalization, close attention should be paid to the risk of invasive aspergillosis upfront. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - February 7, 2024 Category: Microbiology Source Type: research