European Journal of Clinical Microbiology and Infectious Diseases This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

An assessment of the downstream implications of blood culture collection and transit
This study aims to explore the effect of transit times (TT) and culture volume, on time to microbiological diagnosis and patient outcomes. Blood cultures received between 1st March and 31st July 2020/21 were identified. TT, time in incubator (TII), and for positive samples, request to positivity times (RPT) were calculated. Demographic details were recorded for all samples, and culture volume, length of stay (LoS), and 30-day mortality for patients with positive samples. Statistical analysis examined how culture volume and TT effected culture positivity and outcome; in the context of the 4-h national TT target. Totally, 14...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 2, 2023 Category: Microbiology Source Type: research

Antenatal pyelonephritis: a three-year retrospective cohort study of two Irish maternity centres
AbstractPyelonephritis affects 1 –2% of pregnant women, and is associated with significant maternal and fetal morbidity. Antenatal pyelonephritis has been associated with PPROM (preterm premature rupture of membranes), preterm labour, low birth weight (LBW) and prematurity. A three-year retrospective dual-centre cohort study of a ntenatal pyelonephritis cases was conducted in two neighbouring Irish maternity hospitals – the Rotunda Hospital (RH) and the National Maternity Hospital (NMH). Patient demographics, clinical presentation, investigations, management and maternal/neonatal outcomes were recorded. A total of 4...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 1, 2023 Category: Microbiology Source Type: research

Prevalence of latent Mycobacterium tuberculosis infection in hematopoietic stem cell transplantation comparing tuberculin skin test and interferon-gamma release assay
AbstractThe aim of this study was to evaluate the prevalence of latentMycobacterium tuberculosis infection in hematopoietic stem cell transplantation candidates, using tuberculin skin test and QuantiFERON-TB Gold-Plus, in a high-burden tuberculosis country. Adult candidates for hematopoietic stem cell transplantation performed both tests before and those submitted to transplantation were followed up for 12 months. The prevalence of latentMycobacterium tuberculosis infection was 17.1% and a moderate agreement between QuantiFERON-TB Gold-Plus and tuberculin skin test was observed in this population. Previous tuberculosis exp...
Source: European Journal of Clinical Microbiology and Infectious Diseases - May 1, 2023 Category: Microbiology Source Type: research

Incidence, seasonal pattern, and clinical manifestations of Streptococcus dysgalactiae subspecies equisimilis bacteremia; a population-based study
AbstractStreptococcus dysgalactiae subspeciesequisimilis (SDSE) is a human pathogen causing severe invasive infections. Population-based studies on SDSE bacteremia are limited. The purpose of this study was to investigate the incidence, seasonal pattern, clinical manifestations, and recurrence of SDSE bacteraemia. Records regarding patients aged  ≥ 18 years with SDSE bacteremia in the Pirkanmaa health district in August 2015 to July 2018 were retrospectively reviewed. A total of 230 SDSE bacteremia episodes were identified, with 217 episodes (involving 211 patients) available for analysis. The mean annual incidence ...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 29, 2023 Category: Microbiology Source Type: research

Ceftriaxone for methicillin-susceptible Staphylococcus aureus (MSSA) bacteremia: a matter of dosages?
(Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 29, 2023 Category: Microbiology Source Type: research

Ocular thelaziosis: an emerging human parasitosis in Europe
AbstractOcular thelaziosis is a parasitosis distributed mainly in East Asia, but increasingly described in Europe in different domestic and wild animals, including dogs, different wild canids and lagomorphs, and exceptionally in humans. In Spain, in some areas, a high infection prevalence rate has been described in domestic canids, which may lead to an increase in human cases. However, the description of human cases is still exceptional, which suggests that they are probably underdiagnosed. A case of ocular thelaziosis in a 2-year-old girl from an urban environment is described. (Source: European Journal of Clinical Microb...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 28, 2023 Category: Microbiology Source Type: research

Genome-wide association study of hemolytic uremic syndrome causing Shiga toxin-producing Escherichia coli from Sweden, 1994 –2018
This study is aimed at identifying STEC genetic factors associated with the development of HUS in Sweden. A total of 238 STEC genomes from STEC-infected patients with and without HUS between 1994 and 2018 in Sweden were included in this study. Serotypes, Shiga toxin gene (stx) subtypes, and virulence genes were characterized in correlation to clinical symptoms (HUS and non-HUS), and pan-genome wide association study was performed. Sixty-five strains belonged to O157:H7, and 173 belonged to non-O157 serotypes. Our study revealed that strains of O157:H7 serotype especially clade 8 were most commonly found in patients with HU...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 27, 2023 Category: Microbiology Source Type: research

Serological diagnosis of toxoplasmosis in pregnancy: comparison between a manual commercial ELISA assay and the automated VIDAS ® kit
AbstractKnowledge of the toxoplasmosis serological status in pregnant women is important to allow adequate management for the prevention of congenital toxoplasmosis of those who are not immunized. Serological screening is generally carried out using commercial kits to determine the presence or absence of immunoglobulins M or G in the maternal blood. Robust results are therefore needed. We evaluated the performances of a commercial ELISA assay composed of several recombinant parasite antigens and of a commercial assay using parasite lysate to determine the serological status againstToxoplasma gondii of African pregnant wome...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 25, 2023 Category: Microbiology Source Type: research

A ring trial to harmonize Toxoplasma gondii microsatellite typing: comparative analysis of results and recommendations for optimization
AbstractA ring trial among five European laboratories was organized to reach consistency in microsatellite (MS) typing of the zoonotic parasiteToxoplasma gondii. Three sample sets were circulated and analyzed by each laboratory following a previously published method that is based on fragment length polymorphism of 15 MS markers. The first sample set compared typing results in general and focused on effects of DNA concentration; the second sample set focused on the polymorphic fingerprinting markers that can differentiateT. gondii strains within the same archetypal lineage; and the third set focused on non-archetypal genot...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 24, 2023 Category: Microbiology Source Type: research

Chlorhexidine susceptibility and Eagle effect in planktonic cells and biofilm of nosocomial isolates
AbstractThe aim of this study is to evaluate the chlorhexidine gluconate (CHG) susceptibility in both planktonic cells and biofilm of 32 Gram-negative (Gn) and 6 Gram-positive (Gp) isolates by minimal inhibitory concentration (2 –256 μg/mL for Gn and 2–32 μg/mL for Gp), minimal bactericidal concentration (4–256 μg/mL for Gn and 2–32 μg/mL for Gp) in planktonic cells, and minimal biofilm elimination concentration (128 ≥ 16,384 μg/mL in Gn and 32 ≥ 16,384 μg/mL in Gp) in biofilm environment. Ou r study showed that Gn isolates have higher minimal concentrations than Gp and bacteria in biofilms ...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 22, 2023 Category: Microbiology Source Type: research

Genomic characterization of emerging invasive Streptococcus agalactiae serotype VIII in Alberta, Canada
Abstract Invasive Group B Streptococcus (GBS) can infect pregnant women, neonates, and older adults. Invasive GBS serotype VIII is infrequent in Alberta; however, cases have increased in recent years. Here, genomic analysis was used to characterize fourteen adult invasive serotype VIII isolates from 2009 to 2021. Trends in descriptive clinical data and antimicrobial susceptibility results were evaluated for invasive serotype VIII isolates from Alberta. Isolate genomes were sequenced and subjected to molecular sequence typing, virulence and antimicrobial resistance gene identification, phylogenetic analysis, and pangenom...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 21, 2023 Category: Microbiology Source Type: research

Development of subfamily-based consensus PCR assays for the detection of human and animal herpesviruses
AbstractConsensus PCR assays that can be used to sensitively detect several herpesvirus (HV) species across the different subfamilies were developed in this study. Primers containing degenerate bases were designed to amplify regions of the DNA polymerase (DPOL) gene of alpha- and gamma-HVs, and the glycoprotein B (gB) gene of beta-HVs in a singleplex, non-nested touchdown PCR format. The singleplex touchdown consensus PCR (STC-PCR) was used to amplify the DNA of eight human and 24 animal HVs. The assay was able to detect the lowest DNA dilution of 10−5 for alpha-HVs and 10−3 for beta- and gamma-HVs. In comparison, lowe...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 21, 2023 Category: Microbiology Source Type: research

Risk of hepatitis B reactivation following baricitinib or tocilizumab for treatment of COVID-19
(Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 21, 2023 Category: Microbiology Source Type: research

Undiagnosed and missed active pulmonary tuberculosis during mass gatherings: a prospective cross-sectional study from the Hajj pilgrimage
AbstractMass gatherings increase the risk of infectious diseases transmission including tuberculosis (TB). The Hajj pilgrimage to Mecca, Saudi Arabia, is attended by over 2 million pilgrims many of whom are from high TB-burden countries, and has been linked to increased risk of TB acquisition among travellers. We investigated the burden of undiagnosed and missed active pulmonary TB (PTB) among Hajj pilgrims symptomatic for cough. The study was conducted among hospitalised and non-hospitalised travellers attending the Hajj pilgrimage in 2016 and 2017. Questionnaires were used to collect relevant data and sputum samples were...
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 19, 2023 Category: Microbiology Source Type: research

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(Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 18, 2023 Category: Microbiology Source Type: research