Reactive metabolite of trovafloxacin activates inflammasomes: Implications for trovafloxacin ‐induced liver injury
We examined whether trovafloxacin induces the release of damage-associated molecular patterns (DAMPs) that activate inflammasomes. We also tested ciprofloxacin, levofloxacin, gatifloxacin, and grepafloxacin for their ability to activate inflammasomes. Drug bioactivation was performed with human hepatocarcinoma functional liver cell-4 (FLC-4) cells, and THP-1 cells (human monocyte cell line) were used for the detection of inflammasome activation. The supernatant from the incubation of trovafloxacin with FLC-4 cells for 7 days increased caspase-1 activity and production of IL-1ß by THP-1 cells. In the supernatant of FLC-4...
Source: Journal of Applied Toxicology - January 31, 2024 Category: Toxicology Authors: Saori Tanaka,
Takumi Noda,
Kazuya Urashima,
Yoshio Ijiri,
Yuka Kohda,
Ryuji Kato Tags: RESEARCH ARTICLE Source Type: research
Molecules, Vol. 29, Pages 662: Boosted Electrocatalytic Degradation of Levofloxacin by Chloride Ions: Performances Evaluation and Mechanism Insight with Different Anodes
This study elucidates the mechanism of Cl− participation in the electrocatalytic degradation of chlorine-containing organic wastewater. Therefore, the highly chlorine-resistant electrocatalytic anode materials hold great potential for the promotion of the practical application of the electrocatalytic treatment of antibiotic wastewater. (Source: Molecules)
Source: Molecules - January 31, 2024 Category: Chemistry Authors: Keda Yang Peiwei Han Yinan Liu Hongxia Lv Xiaofei Chen Yihan Lei Lian Yu Lei Ma Pingzhou Duan Tags: Article Source Type: research
Antibiotic resistance in Helicobacter pylori among children and adolescents in East Asia: A systematic review and meta-analysis
CONCLUSIONS: H. pylori isolates from East Asian children and adolescents exhibit high levels of metronidazole and clarithromycin resistance, particularly in the Chinese mainland. The non-negligible rates of dual and multiple resistance highlight the complexity of this problem.REGISTRATION: PROSPERO, No. IDCRD42023402510.PMID:38230488 | DOI:10.1097/CM9.0000000000002884 (Source: Chinese Medical Journal)
Source: Chinese Medical Journal - January 17, 2024 Category: General Medicine Authors: Yuhang Zhou Yanli Zhang Shiyu Du Source Type: research
Efficacy of delafloxacin alone and in combination with cefotaxime against cefotaxime non-susceptible invasive isolates of Streptococcus pneumoniae
CONCLUSIONS: CNSSP showed a susceptibility to delafloxacin of 76.7%. Synergistic interactions between delafloxacin and cefotaxime were observed in vitro among CNSSP by GDS cross method.PMID:38226580 | DOI:10.37201/req/107.2023 (Source: Revista Espanola de Quimioterapia)
Source: Revista Espanola de Quimioterapia - January 16, 2024 Category: Drugs & Pharmacology Authors: E R íos M P érez J C Sanz A Delgado-Iribarren I Rodr íguez-Avial Source Type: research
The Percentage of Antibiotic Resistance in Uncomplicated Community-Acquired Urinary Tract Infections —Findings of the RedAres Project
CONCLUSION: For single episodes of uUTI, all of the antibiotics studied can be recommended, at least as far as their resistance profiles are concerned. For recurrent UTI, all but trimethoprim and cotrimoxazole can be recommended. The second-choice antibiotics examined do not have a more favorable resistance profile than the first-choice antibiotics.PMID:38221865 | DOI:10.3238/arztebl.m2023.0267 (Source: Deutsches Arzteblatt International)
Source: Deutsches Arzteblatt International - January 15, 2024 Category: General Medicine Authors: Anja Klingeberg Niklas Willrich Marc Schneider Guido Schmiemann Ildik ó Gágyor Doreen Richter Ines Noll Tim Eckmanns Source Type: research
Molecules, Vol. 29, Pages 396: Chloramine Disinfection of Levofloxacin and Sulfaphenazole: Unraveling Novel Disinfection Byproducts and Elucidating Formation Mechanisms for an Enhanced Understanding of Water Treatment
This study conducts a comprehensive analysis of reaction pathways, encompassing piperazine ring opening/oxidation, Cl-substitution, OH-substitution, desulfurization, and S−N bond cleavage, during chloramine disinfection. Twelve new DBPs were identified in this study, exhibiting stability and persistence even after 24 h of disinfection. Additionally, an examination of DBP generation under varying disinfectant concentrations and pH values revealed peak levels at a molar ratio of 25 for LEV and SPN to chloramine, with LEV contributing 11.5% and SPN 23.8% to the relative abundance of DBPs. Remarkably, this resear...
Source: Molecules - January 13, 2024 Category: Chemistry Authors: Zhenkun Sun Zhenyi Chen Marie Celine Chung Lan Mow Xiaowen Liao Xiaoxuan Wei Guangcai Ma Xueyu Wang Haiying Yu Tags: Article Source Type: research
Machine learning and feature extraction for rapid antimicrobial resistance prediction of Acinetobacter baumannii from whole-genome sequencing data
ConclusionOur study is the first to demonstrate that AMR prediction for A. baumannii using machine learning methods based on k-mer features has competitive performance over traditional workflows; hence, sequence-based AMR prediction and its application could be further promoted. The k-mer-based workflow developed in this study demonstrated high recall/sensitivity and specificity, making it a dependable tool for MIC prediction in clinical settings. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - January 11, 2024 Category: Microbiology Source Type: research
Characterization of a 3-dimensional tuberculosis spheroid model constructed using human monocytic THP-1 cells and < em > Bacillus Calmette-Guerin < /em >
CONCLUSION: The 3-D tuberculous spheroid model established in this study provides a useful platform for studies of tuberculous granuloma.PMID:38189396 | DOI:10.12122/j.issn.1673-4254.2023.12.14 (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - January 8, 2024 Category: Universities & Medical Training Authors: J Guo Y Qiu C Hu D Li Y DU Source Type: research
Characterization of a 3-dimensional tuberculosis spheroid model constructed using human monocytic THP-1 cells and < em > Bacillus Calmette-Guerin < /em >
CONCLUSION: The 3-D tuberculous spheroid model established in this study provides a useful platform for studies of tuberculous granuloma.PMID:38189396 | PMC:PMC10774115 | DOI:10.12122/j.issn.1673-4254.2023.12.14 (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - January 8, 2024 Category: Universities & Medical Training Authors: J Guo Y Qiu C Hu D Li Y DU Source Type: research
Pyridylpiperazine efflux pump inhibitor boosts in vivo antibiotic efficacy against K. pneumoniae
EMBO Mol Med. 2023 Dec 20. doi: 10.1038/s44321-023-00007-9. Online ahead of print.ABSTRACTAntimicrobial resistance is a global problem, rendering conventional treatments less effective and requiring innovative strategies to combat this growing threat. The tripartite AcrAB-TolC efflux pump is the dominant constitutive system by which Enterobacterales like Escherichia coli and Klebsiella pneumoniae extrude antibiotics. Here, we describe the medicinal chemistry development and drug-like properties of BDM91288, a pyridylpiperazine-based AcrB efflux pump inhibitor. In vitro evaluation of BDM91288 confirmed it to potentiate the ...
Source: Molecular Medicine - January 4, 2024 Category: Molecular Biology Authors: Anais Vieira Da Cruz Juan-Carlos Jim énez-Castellanos Clara B örnsen Laurye Van Maele Nina Compagne Elizabeth Pradel Reinke T M üller Virginie Meurillon Daphn ée Soulard Catherine Piveteau Alexandre Biela Julie Dumont Florence Leroux Benoit Deprez Nic Source Type: research
Vibrio species: development of EUCAST susceptibility testing methods and MIC and zone diameter distributions on which to determine clinical breakpoints
ConclusionsWe demonstrated the validity of using standard BMD and EUCAST disc diffusion methodology for AST of fiveVibrio spp., and generated suitable data to allow EUCAST to determine clinical MIC and zone diameter breakpoints for five pathogenicVibrio spp., including both non-toxigenic and toxigenicV. cholerae. (Source: Journal of Antimicrobial Chemotherapy)
Source: Journal of Antimicrobial Chemotherapy - December 30, 2023 Category: Microbiology Source Type: research
Effects of Apex Size on Dissolution Profiles in the USP II Paddle Apparatus
This study aimed to clarify how different apex vessel sizes affect the dissolution behavior of cone-forming formulations. Five apex vessels with different heights, centralities, and compendial vessels were used. The paddle rotation speed at which the coning phenomenon resolved was measured using standard particles of different densities. Three model formulations-USP prednisone tablets, atorvastatin calcium hydrate tablets, and levofloxacin fine granules-were selected, and dissolution tests were conducted at 30-100 revolutions per minute (rpm). Compared to the compendial vessels, the disappearance of standard particles at t...
Source: AAPS PharmSciTech - December 29, 2023 Category: Drugs & Pharmacology Authors: Hiroyuki Yoshida Tokio Morita Yasuhiro Abe Aoi Inagaki Naomi Tomita Ken-Ichi Izutsu Yoji Sato Source Type: research
Effects of Apex Size on Dissolution Profiles in the USP II Paddle Apparatus
This study aimed to clarify how different apex vessel sizes affect the dissolution behavior of cone-forming formulations. Five apex vessels with different heights, centralities, and compendial vessels were used. The paddle rotation speed at which the coning phenomenon resolved was measured using standard particles of different densities. Three model formulations-USP prednisone tablets, atorvastatin calcium hydrate tablets, and levofloxacin fine granules-were selected, and dissolution tests were conducted at 30-100 revolutions per minute (rpm). Compared to the compendial vessels, the disappearance of standard particles at t...
Source: AAPS PharmSciTech - December 29, 2023 Category: Drugs & Pharmacology Authors: Hiroyuki Yoshida Tokio Morita Yasuhiro Abe Aoi Inagaki Naomi Tomita Ken-Ichi Izutsu Yoji Sato Source Type: research
Antibiotic sensitivity of environmental Legionella pneumophila strains isolated in Poland
CONCLUSIONS: The lowest biocidal concentration was found for levofloxacin, the highest for tetracycline. The highest MIC50 and MIC90 values were found for tetracycline and the lowest for rifampicin. The highest biocidal values were found for azithromycin and the lowest for tetracycline.PMID:38153060 | DOI:10.26444/aaem/167934 (Source: Annals of Agricultural and Environmental Medicine : AAEM)
Source: Annals of Agricultural and Environmental Medicine : AAEM - December 28, 2023 Category: Environmental Health Authors: Iwona G ładysz Agnieszka Sikora Ma łgorzata Wójtowicz-Bobin Adam Szepeluk Renata Matuszewska Marta Bartosik Jan Kazimierz Karczewski Source Type: research
