[Dihydroartemisinin alleviates atopic dermatitis in mice by inhibiting mast cell infiltration].
Abstract OBJECTIVE: To observe the therapeutic effect of different doses of dihydroartemisinin (DHA) on atopic dermatitis (AD) in mice and explore the mechanism. METHODS: Forty-two C57BL/6 mice were randomly divided into 7 groups (n=6), including a blank control group, a 2, 4-dinitrochlorobenzene (DNCB)-induced AD model group, a solvent-treated group, 3 DHA treatment groups treated with 25, 75, and 125 mg/kg DHA, and a dexamethasone treatment group. The counts of skin scratches were recorded and the lesion scores were evaluated on a daily basis. After 7 consecutive days of treatment, skin tissues were sampled...
Source: Journal of Southern Medical University - October 30, 2020 Category: Universities & Medical Training Authors: Xue X, Dong Z, Deng Y, Yin S, Wang P, Liao Y, Hu G, Chen Y Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Single nucleotide polymorphism of rs28416520 in Piwil1 gene promoter region is associated with an increased risk of gastric cancer].
Abstract OBJECTIVE: To analyze the correlation between the single nucleotide polymorphisms (SNPs) in the promoter of Piwil1 gene and gastric cancer. METHODS: The expression of Piwil1 mRNA in the tumor tissues of 3 patients with gastric cancer was detected by RT-qPCR, and RNA-Sequencing data from the Cancer RNA-Seq Nexus were analyzed for Piwil1 mRNA expression in gastric patients. Blood samples were collected from 24 gastric cancer patients and 29 healthy control subjects for PCR amplification of Piwil1 gene promoter region. The SNP loci in the promoter region of Piwil1 gene were determined by direct sequenci...
Source: Journal of Southern Medical University - October 30, 2020 Category: Universities & Medical Training Authors: Zhenzhen LI, Lanting Z, Lihong Z, Juan X, Zhengjiang C Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[High-salt exposure induces macrophage polarization to promote proliferation and phenotypic transformation of co-cultured renal fibroblasts].
Abstract OBJECTIVE: To investigate high-salt exposure-induced polarization of mononuclear macrophages and the changes in proliferation and phenotypic transformation of renal fibroblasts in a co-culture system. METHODS: Cultured mononuclear macrophages were exposed to high salt (161 mmol/L Na +) for 2 h and the surface markers of M0, M1 and M2-type macrophages were detected with RT-qPCR. The culture medium of the macrophages in normal and high-salt groups was collected for detection of the mRNA and protein levels of IL-6 and TGF-β1 using RT-qPCR and ELISA. A co-culture system of high salt-exposed macropha...
Source: Journal of Southern Medical University - October 30, 2020 Category: Universities & Medical Training Authors: Lu J, Bai Z, Kuang X, Li L Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Esophageal pH-impedance monitoring of reflux patterns in non-erosive reflux disease, reflux hypersensitivity and functional heartburn].
Abstract OBJECTIVE: To analyze the differences in reflux patterns in 24-hour esophageal pH-impedance monitoring in patients with non-erosive reflux disease (NERD), reflux hypersensitivity (RH) and functional heartburn (FH) and explore the possible mechanism of symptoms in patients with heartburn and negative endoscopic findings. METHODS: Seventy-nine patients with heartburn as the main symptoms but negative endoscopic findings, including 35 with NERD, 16 with RH and 28 with FH, were enrolled in this study.All the patients underwent 24-h esophageal pH-impedance monitoring and esophagogastroscopy, and the resul...
Source: Journal of Southern Medical University - October 30, 2020 Category: Universities & Medical Training Authors: Zhang A, Yao W, Di J, Cui M, Zhang M, Zhang J Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Intragastric balloon for weight reduction: rationale, benefits, risks and indications].
Abstract Intragastric balloon (IGB) placement under endoscopy is a non-invasive method for weight loss.By placing a space-occupying balloon in the stomach, IGB treatment can achieve better effect of weight loss than medications.Herein we review the development of IGB, its effect on weight loss and the mechanism, and the eligible individuals for IGB treatment.We also examine the high-intensity postoperative management following IGB placement, which is important for maintaining long-term weight loss, and discuss the future development of IGB.The patients should understand that on the basis of ensuring a high safety,...
Source: Journal of Southern Medical University - October 30, 2020 Category: Universities & Medical Training Authors: Wu L, Dai X, Zhao W, Huang H, Mo L, Wu X Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Mental health status and its influencing factors among general population and medical personnel in Guangdong Province during COVID-19 pandemic].
CONCLUSIONS: Psychological interventions are essential for both the general population and healthcare professionals, especially for unmarried individuals in the general population and the nursing staff.An excessive exposure to COVID-19-related information may have detrimental effects on the mental health.For healthcare professionals, sufficient rest needs to be ensured, and education programs on COVID-19 should be implemented among both residents and healthcare professionals to improve their mental health. PMID: 33118506 [PubMed - in process] (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - October 30, 2020 Category: Universities & Medical Training Authors: Liu Z, Zhang X, Lü Z, Liang J, Deng Y, Feng L Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Success rate of one-stop procedure for atrial fibrillation ablation and its impact on cardiac function: a propensity-matched study].
Abstract OBJECTIVE: To investigate the effect of the combination of atrial fibrillation (AF) ablation and left atrial appendage closure (LAAC) on cardiac function and the success rate of AF ablation. METHODS: We retrospectively analyzed the data of 56 patients with AF undergoing a one-stop procedure for AF ablation and LAAC in our hospital between May, 2015 and May, 2019. Propensity score matching (PSM) at the ratio of 1:1 was used to select 56 control patients undergoing AF ablation at high risk of stroke, for matching with the hybrid procedure group. The perioperative complications, thromboembolic events, r...
Source: Journal of Southern Medical University - October 30, 2020 Category: Universities & Medical Training Authors: Zhu S, Zheng M, Yan R, Tan Z, Zhao H, Zhang J, Peng J Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Clinical value of individualized pharmaceutical services for patients receiving vancomycin for severe infections: a retrospective case-control study based on real-world data].
Abstract OBJECTIVE: To assess the clinical value of individualized pharmaceutical services for patients receiving vancomycin for severe infections and establish clinical monitoring procedures during vancomycin treatment. METHODS: Data were collected from patients with severe infections who received vancomycin treatment with individualized pharmacy services (test group, 144 cases) or without such services (control group, 884 cases) between January, 2017 and December, 2018. Using propensity score matching, the patients in the two groups with comparable baseline data were selected for inclusion in the study (62 ...
Source: Journal of Southern Medical University - October 30, 2020 Category: Universities & Medical Training Authors: Chen H, Shi Q, Kong L, Sang R, Yu M, Sun J, Zhu Y, Zhu J Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[miR-133b inhibits myocardial ischemia-reperfusion-induced cardiomyocyte apoptosis and accumulation of reactive oxygen species in rats by targeting YES1].
Abstract OBJECTIVE: To investigate the effect of miR-133b on cardiomyocyte apoptosis induced by myocardial ischemia-reperfusion (I/R) and explore the mechanism. METHODS: Thirty-six adult SD rats were randomized into sham-operated group, I/R group, AdmiR-NC group and AdmiR-133b group, and rat models of myocardial I/R were established in the latter 3 groups with myocardial injections of saline or recombinant adenoviruses in the left ventricle. The expression of MiR-133b was detected using RT-qPCR, and cardiac function of the rats was determined using FDP 1 HRV and BRS analysis system. Serum CK-MB and cTnI level...
Source: Journal of Southern Medical University - October 30, 2020 Category: Universities & Medical Training Authors: Peng X, Lin L, Zhou X, Yang D, Cao Y, Yin T, Liu Y Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Ponatinib inhibits growth of patient-derived xenograft of cholangiocarcinoma expressing FGFR2-CCDC6 fusion protein in nude mice].
Abstract OBJECTIVE: To investigate the antitumor effect of ponatinib on the growth of cholangiocarcinoma xenograft derived from a clinical patient in a mouse model expressing FGFR2-CCDC6 fusion protein. METHODS: Lung metastatic tumor tissue was collected from a patient with advanced intrahepatic cholangiocarcinoma and implanted subcutaneously a NOD/SCID/ Il2rg-knockout (NSG) mouse. The tumor tissues were harvested and transplanted in nude mice to establish mouse models bearing patient-derived xenograft (PDX) of cholangiocarcinoma expressing FGFR2-CCDC6 fusion protein. The PDX mouse models were divided into 4 ...
Source: Journal of Southern Medical University - October 30, 2020 Category: Universities & Medical Training Authors: Wu T, Jiang X, Xu B, Wang Y Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Spindle assembly checkpoint complex-related genes TTK and MAD2L1 are over-expressed in lung adenocarcinoma: a big data and bioinformatics analysis].
CONCLUSIONS: Seven SAC complex-related genes, including TTK and MAD2L1, are overexpressed in lung adenocarcinoma tissues with close correlation with the prognosis of the patients. PMID: 33118511 [PubMed - in process] (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - October 30, 2020 Category: Universities & Medical Training Authors: Liu Z, Guo Z, Long L, Zhang Y, Lu Y, Wu D, Dong Z Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Menthol enhances interleukin-13-induced synthesis and secretion of mucin 5AC in human bronchial epithelial cells].
Abstract OBJECTIVE: To investigate the effect of interleukin (IL) -13 combined with cold stimulation on synthesis and secretion of mucin (MUC) 5AC in human bronchial epithelial cell line 16HBE and explore the role of transient receptor potential 8 (TRPM8) and anti-apoptotic factor B-cell lymphoblast-2 (Bcl-2) in this process. METHODS: 16HBE cells were stimulated with 10 ng/mL IL-13, 1 mmol/L menthol, or both (1 mmol/L menthol was added after 6 days of IL-13 stimulation), and the changes in the expression of MUC5AC, intracellular Ca2+ concentration and Bcl-2 expression were evaluated. The effects of ABT-263 (a...
Source: Journal of Southern Medical University - October 30, 2020 Category: Universities & Medical Training Authors: Zhang M, Wang J, Li M Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Effect of high-fat diet and exercise on asprosin and CTRP6 expression in subcutaneous and retroperitoneal adipose tissues in rats during mid-gestation].
Abstract OBJECTIVE: To study the effects of high-fat (HF) diet and exercise on the expressions of asprosin and CTRP6 in adipose tissues in different regions of rats during mid-gestation. METHODS: Pregnant SD rats were fed on a standard chow diet or a high-fat (60% fat content) diet for 14 days starting on gestation day (GD) 1. Starting from GD3, the rats fed either on normal or high-fat diet in the exercise groups (CH-RW and HF-RW groups) were allowed access to the running wheels for voluntary running, and those in sedentary groups (CH-SD and HF-SD groups) remained sedentary. At the end of the 14 days, adipos...
Source: Journal of Southern Medical University - October 30, 2020 Category: Universities & Medical Training Authors: Yang Z, Jiang J, Huang J, Zhao Y, Luo X, Song L Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Electro-acupuncture promotes repair of rotator cuff injury in rats].
Abstract OBJECTIVE: To observe the therapeutic effect of electro-acupuncture on tendon healing and functional recovery of rotator cuff injury in rats and explore the therapeutic mechanism of electro-acupuncture. METHODS: Ninety SD rats were randomly divided into electro-acupuncture group, model group and blank control group, and models of rotator cuff injury were established in the former two groups.The rats in electro-acupuncture group was treated with electro-acupuncture after the operation, and those in the other two groups received no treatment.The right forefoot thermal withdrawal latency (TWL), the cont...
Source: Journal of Southern Medical University - October 30, 2020 Category: Universities & Medical Training Authors: Song W, Han X, Li K, Chen C, Wang H, Zheng X Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Pathogenic role leukotriene B4 in lung injury induced by lung-protective mechanical ventilation in rabbits].
Abstract OBJECTIVE: To elucidate the pathogenic role of leukotriene B4 (LTB4) in pulmonary hyper-permeability and inflammation induced by lung-protective mechanical ventilation (LPMV) in rabbits. METHODS: Thirty-two healthy Japanese white rabbits were randomized into 4 groups for treatment with vehicle or bestatin (a leukotriene A4 hydrolase inhibitor that inhibits LTB4 production) administered intragastrically at the daily dose of 8 mg/kg for 5 days, followed by sham operation (group S and group BS, respectively, in which the rabbits were anesthetized only) or LPMV (group PM and group BPM, respectively, in w...
Source: Journal of Southern Medical University - October 30, 2020 Category: Universities & Medical Training Authors: Yuan L, Li J, Yang Y, Guo X, Liu X, Li L, Zhu X, Liu R Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Discrimination of lung cancer and adjacent normal tissues based on permittivity by optimized probabilistic neural network].
CONCLUSIONS: Compared with the traditional probabilistic neural network, BP neural network, RBF neural network and the classification discriminant analysis function (Classify) in MATLAB, the proposed SA-PNN has higher accuracy, sensitivity and specificity for discriminating lung cancer and the adjacent normal tissues based on permittivity. PMID: 33118516 [PubMed - in process] (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - October 30, 2020 Category: Universities & Medical Training Authors: Yu H, Sun Y, Lu D, Cai K, Yu X Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Clinical analysis of early damage in multiple extra-pulmonary organs in COVID-19].
CONCLUSIONS: In patients with COVID-19, heart damage can be identified early by observing the GLS and new abnormalities on ECG in spite of normal aTNI and LVEF.Early liver injury is not obvious in these patients, but dynamic monitoring of the indicators of should be emplemented, especially in severe cases. In cases with normal CR and BUN, kidney damage can be detected early by calculating eGFR, Ccr and UACR and urine protein tests. PMID: 33118517 [PubMed - in process] (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - October 30, 2020 Category: Universities & Medical Training Authors: Fan J, Zhang Y, Pan Z, Wang L, Hong X, Wu L, Guo S Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Zoledronate regulates osteoclast differentiation and bone resorption in high glucose through p38 MAPK pathway].
Abstract OBJECTIVE: To investigate the effect of zoledronate (ZOL) on osteoclast differentiation and bone resorption under high glucose, and the regulation mechanism of p38 mitogen activated kinase (p38 MAPK) signaling pathway in this process. METHODS: RAW264.7 cells were divided into four groups: low group, high group, low+ZOL group and high+ZOL group after induced into osteoclasts. Cell proliferation activity was determined by MTT assay. The migration of RAW264.7 cells were examined Optical microscopy. Immunofluorescence microscopy was used to observe the cytoskeleton and sealing zones of osteoclasts. After...
Source: Journal of Southern Medical University - October 30, 2020 Category: Universities & Medical Training Authors: Lin Y, Gu Y, Zuo G, Jia S, Liang Y, Qi M, Dong W Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Effect of ozone oil for prevention and treatment of sorafenib-induced hand-foot skin reactions: a randomized controlled trial].
Abstract OBJECTIVE: To compare the effects of medical ozone oil and urea ointment for prevention and treatment of hand-foot skin reaction (HFSR) caused by sorafenib in patients with hepatocellular carcinoma (HCC). METHODS: A total of 99 patients diagnosed with advanced HCC according to National Comprehensive Cancer Network (NCCN) who were scheduled to receive sorafenib treatment for the first time were enrolled in this study between April, 2018 and January, 2020. The patients were randomized into medical ozone oil group (n=49) and urea ointment group (control group, n=49) for treatment with local application ...
Source: Journal of Southern Medical University - October 30, 2020 Category: Universities & Medical Training Authors: Chen X, Jiang Y, Zhang Y, Dai W, Fan R, Weng X, He P, Yan F, Guo Y Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Correlation of type 2 diabetes and impaired glucose regulation with chronic kidney disease in middle-aged and elderly individuals].
Abstract OBJECTIVE: To explore the correlation of different glucose metabolism statues with chronic kidney disease (CKD) in middle-aged and elderly individuals in Lanzhou. METHODS: Based on the baseline data of REACTION Study in Lanzhou area, we randomly sampled 10 038 residents aged 40-75 years in 3 communities in Lanzhou, who were classified into normal glucose tolerance (NGT), impaired glucose regulation (IGR) and diabetes groups. The estimated glomerular filtration rate (eGFR) and urinary albumin-to-creatinine ratio (ACR) were used to assess the renal function and albuminuria, respectively. Binary logisti...
Source: Journal of Southern Medical University - October 30, 2020 Category: Universities & Medical Training Authors: Wang Q, Zhen J, Guan C, Zhao N, Liu J, Li H, Fu S, Tang X, Han Y, Su S, Zhang D, Liu L, Zhen D Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[An improved association analysis pipeline for tumor susceptibility variant in haplotype amplification area].
CONCLUSIONS: The proposed method can accurately identify tumor susceptibility variants in haplotype amplification area with good robustness and stability. PMID: 33118521 [PubMed - in process] (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - October 30, 2020 Category: Universities & Medical Training Authors: Geng Y, Yang R, Zhang J Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Arctiin antagonizes triptolide-induced renal toxicity in rats via anti-inflammatory pathway].
Abstract OBJECTIVE: To investigate the protective effect of arctiin with anti-inflammatory bioactivity against triptolide-induced nephrotoxicity in rats and explore the underlying mechanism. METHODS: Forty SD rats were divided into 4 groups for gastric lavage of normal saline, arctiin (500 mg/kg), triptolide (500 μg/kg), or both arctiin (500 mg/kg) and triptolide (500 μg/kg). Blood samples were collected for analysis of biochemical renal parameters, and the renal tissues were harvested for determining the kidney index and for pathological evaluation with HE staining. In the in vitro experiment, HK-2 cel...
Source: Journal of Southern Medical University - October 30, 2020 Category: Universities & Medical Training Authors: Zhou Y, Lu X, Xia L, Yao W, Qin G, Wang G Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Long-term clinical outcomes of patients with aneurysmal subarachnoid hemorrhage in Yunnan Province].
Abstract OBJECTIVE: To investigate the clinical outcomes of patients with aneurysmal subarachnoid hemorrhage (aSAH) after surgeries in Yunnan Province. METHODS: We retrospectively analyzed the demographic features, vascular risk factors, severity at admission, and aneurysm locations in 85 patients with aSAH receiving surgical interventions in Yunnan Province. All the patients were treated by aneurysm clipping or coiling and followed up for clinical outcomes and recovery of daily activities evaluated by modified Rankin Scale (mRS) and Activities of Daily Living (ADL) scale, respectively. RESULTS: Thirty-f...
Source: Journal of Southern Medical University - September 29, 2020 Category: Universities & Medical Training Authors: Sun J, Wang Z, Su P, Liu J, Li J, Ma G, Cen J, Chang Q, Liu X, Zhao N Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Long non-coding RNA LINC01106 regulates colorectal cancer cell proliferation and apoptosis through the STAT3 pathway].
Abstract OBJECTIVE: To investigate the expression of LINC01106 in colorectal cancer and its role in regulating the proliferation and apoptosis of colorectal cancer cells. METHODS: We analyzed the data of LINC01106 expression levels in tumor tissues and normal tissues of patients with colorectal cancer in TCGA database and explored the association of LINC01106 expression level with the prognosis of the patients. Colorectal cancer SW480 cell lines with LINC01106 knockdown or overexpression were established, and their proliferation and apoptosis relative to the parental cells were evaluated using CCK-8 assay and...
Source: Journal of Southern Medical University - September 29, 2020 Category: Universities & Medical Training Authors: Gu Y, Huang Y, Sun Y, Liang X, Kong L, Liu Z, Wang L Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Human umbilical cord mesenchymal stem cells differentiate into neuron-like cells after induction with B27-supplemented serum-free medium].
Abstract OBJECTIVE: To evaluate the capacity and efficiency of human umbilical cord mesenchymal stem cells (HUCMSCs) to differentiate into neuron- like cells after induction with B27- supplemented serum- free medium. METHODS: HUCMSCs at passage 4 were cultured for 14 days with serum-containing medium (SCM) (group A), SCM supplemented with 20 ng/mL nerve growth factor (NGF) and 10 ng/mL basic fibroblast growth factor (bFGF) (group B), serum-free medium (SFM) (group C), or SFM supplemented with 20 ng/mL NGF and 10 ng/mL bFGF. The culture medium were changed every 3 days and the growth of the neurospheres was ob...
Source: Journal of Southern Medical University - September 29, 2020 Category: Universities & Medical Training Authors: Li Y, Yang J, Fu G, Zhou P, Liu Y, Li Z, Jiao G Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[MicroRNA-200c-3p inhibits proliferation of nephroblastoma cells by targeting CCNE2].
Abstract OBJECTIVE: To predict and verify the target gene of miR-200c-3p and evaluate the inhibitory effect of miR-200c-3p on the proliferation of nephroblastoma cells. METHODS: The putative target genes of miR-200c-3p were predicted by bioinformatics approach. Nephroblastoma cell models with miR-200c-3p overexpression or knockdown were established in SK-NEP-1 and G401 cells with corresponding control groups. The expressions of CCNE2 in SK-NEP-1 and G401 cells in different groups were detected by RT-PCR and Western blotting. A luciferase reporter assay was used to determine the targeting relationship between ...
Source: Journal of Southern Medical University - September 29, 2020 Category: Universities & Medical Training Authors: Cao J, Sun L, An J, Zhang H, He X, Shen H Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Expression of DNMT3b in human bladder cancer tissue and its correlation with clinical prognosis].
Abstract OBJECTIVE: To investigate the expression of DNMT3b in human bladder cancer tissues and its correlation with postoperative survival of patients with bladder cancer. METHODS: Thirty-eight pairs of surgically resected human bladder cancer tissues and adjacent bladder tissues were detected by immunohistochemistry for DNMT3b expression, and the correlations of DNMT3b expression level were analyzed with the patients'age, gender, pathological grade, tumor size, T stage, lymph node metastasis and TNM stages. Kaplan-Meier survival analysis was performed to assess the effect of DNMT3b expression on survival ou...
Source: Journal of Southern Medical University - September 29, 2020 Category: Universities & Medical Training Authors: Cao Y, Xu K, Chen B, Wang Y, Li B, Li C, Xu P Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Alkaline processing of cantharidin can significanty improve the antitumor activity of cantharidin].
Abstract OBJECTIVE: To assess the changes in the effects of cantharides after alkaline processing on proliferation, migration, invasion, and apoptosis of human lung cancer A549 cells. METHODS: Human non-small cell lung cancer A549 cells were treated with cantharis extract (CTE) from raw cantharides and alkali processed cantharis extract (ACE). The proliferation of the cells was detected with CCK-8 assay, and the cell migration and invasion were assessed using wound healing assay and Transwell assay, respectively. The expressions of MMP1 and MMP2 in the cells were detected using Western blotting, the contents ...
Source: Journal of Southern Medical University - September 29, 2020 Category: Universities & Medical Training Authors: Li X, Li S, Pang J, Huang F, Guo B, Liu H Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Application of micellar electrokinetic capillary chromatography for identification, quantitative detection and unfolding analysis of interleukin-12].
CONCLUSIONS: This capillary electrophoresis-based method is simple and sensitive for IL-2 analysis and allows rapid detection of changes in IL-12 content in the setting of tumors and analysis of the possible causes. PMID: 32990226 [PubMed - in process] (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - September 29, 2020 Category: Universities & Medical Training Authors: Bao P, Sun Y, Wang H, Jin X, Zhang G Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[What happened in the acupoint area of mouse after blood injection?]
CONCLUSIONS: Blood injection at the acupoints can mimic the process of hematoma absorption in the muscular tissue, and local inflammation is initiated to engulf and clear the blood components, which may be the basis for the therapeutic effects induced by acupoint stimulation. PMID: 32990227 [PubMed - in process] (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - September 29, 2020 Category: Universities & Medical Training Authors: Yong C, Yongpu L, Sufen B, Yao T, Fujuan Q, Changhong X Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Analysis of intestinal flora in patients with chronic rhinosinusitis based on highthroughput sequencing].
Abstract OBJECTIVE: To investigate the changes in diversity, relative abundance and distribution of intestinal flora in patients with chronic rhinosinusitis and nasal polyps (CRSwNP) using high-throughput sequencing technology identify the intestinal flora significantly related to pathogenesis and progression of CRSwNP. METHODS: Ten patients with CRSwNP hospitalized in the Department of Otolaryngology-Head and Neck Surgery of Guangdong Provincial People's Hospital were selected as the case group with 10 healthy volunteers recruited in the same period as the control group. Fecal genomic DNA extraction kit was ...
Source: Journal of Southern Medical University - September 29, 2020 Category: Universities & Medical Training Authors: Huang J, Wang L, Wu X, Chen H, Fu X, Chen S, Liu T Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Epigallocatechin gallate induces CHD5 gene demethylation to promote acute myeloid leukemia cell apoptosis in vitro by regulating p19Arf-p53-p21Cip1 signaling pathway].
Abstract OBJECTIVE: To investigate the mechanism by which epigallocatechin gallate (EGCG) induces CHD5 gene demethylation and promotes the apoptosis of acute myeloid leukemia KG-1 and THP-1 cell lines. METHODS: KG-1 and THP-1 cells treated with 25, 50, 75, 100 or 150 μg/mL EGCG for 48 h were examined for CHD5 gene methylation using MSP and for cell proliferation using MTT assay. The changes in cell cycle and apoptosis of the two cell lines after treatment with EGCG for 48 h were detected using flow cytometry. The mRNA and protein expressions of DNMT1, CHD5, p19Arf, p53 and p21Cip1 in the cells were detecte...
Source: Journal of Southern Medical University - September 29, 2020 Category: Universities & Medical Training Authors: Wu M, Jiang M, Xue M, Li Q, Cheng B, Huang M, Xu L, Zhang Y Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Correlation of plasma N-acetyl-neuraminic acid level with TIMI risk stratification and clinical outcomes in patients with acute coronary syndrome].
Abstract OBJECTIVE: To explore the correlation of plasma N-acetyl-neuraminic acid level with Thrombolysis In Myocardial Infarction (TIMI) risk score and clinical outcomes of patients with acute coronary syndrome (ACS). METHODS: We consecutively enrolled 708 consecutive patients (401 male and 307 female, mean age 63.6±10.6 years) undergoing coronary angiography in our hospital between October, 2018 and July, 2019, including 597 patients with ACS and 111 without ACS (control group). The patients with ACS group were divided into high (n=104), moderate (n=425) and low (n=68) risk groups according to their ...
Source: Journal of Southern Medical University - September 29, 2020 Category: Universities & Medical Training Authors: Li M, Qian S, Yao Z, Min S, Shi X, Kang P, Zhang N, Wang X, Gao D, Gao Q, Zhang H, Wang H Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Effect of Straumann implant on crown appearance in immediate implant restoration of maxillary anterior teeth].
This study was conducted among 86 patients undergoing immediate implant restoration of maxillary anterior teeth between January and December, 2018. We randomized the patients into control group for treatment with immediate implant restoration and study group receiving additional Straumann implant. The effects of restoration, bone absorption at 6 months, implant length, implant diameter, root protrusion, gingival color, far and middle gingival papilla, lip side gingival height, near and middle gingival papilla, and lip side gingival curve were compared between the two groups. RESULTS: The repair effect in the study gro...
Source: Journal of Southern Medical University - September 29, 2020 Category: Universities & Medical Training Authors: Yao Z, Jia B, Lin S, Shao J Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Effect of hot water extract of Korean ginseng on neuroblastoma cell parthanatos].
Abstract OBJECTIVE: To explore the effect of pretreatment of neuroblastoma cells with hot water extract of Korean ginseng on MNNG-induced parthanatos and its mechanism. METHODS: Neuroblastoma SH-SY5Y cells were pretreated with 1 mg/L hot water extract of Korean ginseng before induction with 250 μmol/L MNNG for 1 h or 4 h. CCK-8 and cell flow cytometry were used to detect cell survival rate. Western blotting was used to detect the changes in poly(ADP-ribose) (PAR) expression in the treated cells. Immunofluorescence assay was used to detect nuclear distribution of apoptosis-inducing factor (AIF), and flow cy...
Source: Journal of Southern Medical University - September 29, 2020 Category: Universities & Medical Training Authors: Guo Y, Zhang D, Long R, Shan S, Sun Q, Cai B, Wang S Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Aurora kinase-B silencing promotes apoptosis of osteosarcoma 143B cells by ULK1 phosphorylation-induced autophagy].
Abstract OBJECTIVE: To investigate the effect of Aurora kinase B (AURKB) silencing-induced autophagy on apoptosis of osteosarcoma 143B cells and the underlying molecular mechanisms. METHODS: Human osteosarcoma 143B cells were transfected with Lv/shAURKB or the negative control vector Lv/shScrambled followed by treatment with chloroquine (CQ) for 24 h. Western blotting was performed to detect the protein expression levels of AURKB, P62, LC3, cleaved caspase-3, Bcl-2, and P-ULK1Ser555. Transmission electron microscopy and LC3 dual-label fluorescence method were used to trace the autophagosomes in 143B cells to ...
Source: Journal of Southern Medical University - September 29, 2020 Category: Universities & Medical Training Authors: Wu X, Liu J, Song H, Yang Q, Ying H, Liu Z Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Construction and validation of a nomogram for predicting the risk of portal vein thrombosis after splenectomy in patients with hepatitis B cirrhosis].
Abstract OBJECTIVE: To construct and validate an individualized nomogram to predict the probability of occurrence of portal vein thrombosis (PVT) after splenectomy in patients with hepatitis B cirrhosis. METHODS: We retrospectively collected the clinical data from 180 patients with hepatitis B cirrhosis undergoing splenectomy with postoperative anticoagulation therapy during the period from January, 2014 to January, 2020 in our hospital. The patients were randomized into modeling group (n= 120) and validation group (n=60), and the former group was further divided into PVT group (n=49) and non-PVT group (n=71)...
Source: Journal of Southern Medical University - September 29, 2020 Category: Universities & Medical Training Authors: Xu W, Cheng Y, Tu B Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Effect of mild hypothermia on behaviors of rats with intracerebral hemorrhage and the possible mechanism].
Abstract OBJECTIVE: To explore the effect of mild hypothermia on inflammatory response and angiogenesis in brain tissues of rats with intracerebral hemorrhage (ICH) and its possible mechanism for improving behavioral deficits of the rats After ICH. METHODS: A total of 120 healthy male SD rats were randomly divided into sham operation group, ICH group and mild hypothermia group. Rat models of ICH were established in the latter two groups by stereotactic injection of autogenous blood in the brain, and the rats in the sham operation group received injection of normal saline in the same manner. At 15 min after mo...
Source: Journal of Southern Medical University - September 29, 2020 Category: Universities & Medical Training Authors: Peng Y, Lin Y, Hou X, Liao X, Liu J Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Anti-scarring effect of rapamycin following filtering surgery in rabbit eyes].
Abstract OBJECTIVE: To study the effect of rapamycin on scar formation in rabbit eyes following filtering operation and explore the possible mechanism. METHODS: Ninety-six healthy adult rabbits were subjected to trabeculectomy of the left eye and subsequently randomly divided into 4 groups (n=24) for treatment with castor oil (control) or rapamycin (1%, 3%, or 5%) eye drops of the operated eyes 4 times a day. The morphology and function of the filtering blebs of the rabbits were compared at 7, 14, 21 and 28 days after the operation; at each of the time points, 6 rabbits from each group were euthanized for det...
Source: Journal of Southern Medical University - September 29, 2020 Category: Universities & Medical Training Authors: Tai X, Shen Y, Zhao H, Wang Z, Guan W, Kang X, Guo W Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[DNMT1 protein promotes retinoblastoma proliferation by silencing MEG3 gene].
Abstract OBJECTIVE: To investigate whether DNMT1 protein induces retinoblastoma proliferation by silencing MEG3 gene. METHODS: Two retinoblastoma cell lines (HXO-RB44 and SO-RB50) and a normal human retinal pigment epithelial (RPE) cell line were transfected with the plasmid pcDNA-DNMT1 or si-DNMT1 for up-regulating or interference of DNMT1 expression, and with pcDNA-MEG3 or si-MEG3 for up-regulating or interference of MEG3 expression. Western blotting was used to detect the changes in the expression of DNMT1 protein in the transfected cells, and CCK-8 and EdU assays were used to detect the changes in cell pr...
Source: Journal of Southern Medical University - September 29, 2020 Category: Universities & Medical Training Authors: Gao Y, Luo X, Meng T, Zhu M, Tian M, Lu X Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Shikonin induces cell death by inhibiting glycolysis in human testicular cancer I-10 and seminoma TCAM-2 cells].
Abstract OBJECTIVE: To investigate the pattern of shikonin-induced cell death in testicular cancer cell I-10 and seminoma TCAM-2 cells and explore the possible mechanism in light of mitochondrial function and glycolysis. METHODS: I-10 cells treated with 0, 1.2, 1.4 and 1.6 μmol/L shikonin and TCAM-2 cells treated with 0, 0.5, 1 and 1.5 μmol/L shikonin were examined for mitochondrial membrane potential and production of reactive oxygen species (ROS) using JC-1 kit and ROS kit, respectively. The levels of intracellular lactic acid in the cells were detected using a lactic acid kit. The inhibitory effect o...
Source: Journal of Southern Medical University - September 29, 2020 Category: Universities & Medical Training Authors: Yao Y, Zhang C, Han B, Tang Y, Xiong Y, Wang S Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Investigation of anxiety and depression in patients from the emergency department during COVID-19 epidemic].
Abstract OBJECTIVE: To investigate the status of anxiety and depression in patients requiring emergency treatment during the epidemic of COVID-19 to identify the patients with acute psychological stress disorder. METHODS: During the COVID-19 epidemic, the medical staff divided the patients visiting the emergency department into suspected group, fever group and control group through interview of the patients at triage. Self-rating anxiety scale (SAS) and self-rating depression scale (SDS) were distributed to each patient, and a trained medical staff was responsible for assisting the patient to complete the sca...
Source: Journal of Southern Medical University - September 29, 2020 Category: Universities & Medical Training Authors: Wang Q, Cao X, Wu X, Liu J, Xie J, Hou D Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Screening potential Chinese materia medica and their monomers for treatment diabetic nephropathy based on caspase-1-mediated pyroptosis].
Abstract OBJECTIVE: To screen potential traditional Chinese medicine and their active monomer ingredients for treatment of diabetic nephropathy (DN) through the mechanism of caspase-1-mediated pyroptosis. METHODS: Using the Chinese Medicine System Pharmacology Analysis Platform (TCMSP), we screened traditional Chinese drugs and their active monomer components targeting caspase-1, and searched for the potential gene targets of the monomer components using GeneCards database. Cytoscape was used to construct the monomer compound-gene target network. Gene ontology (GO) functional enrichment analysis and Kyoto Gen...
Source: Journal of Southern Medical University - September 29, 2020 Category: Universities & Medical Training Authors: Zhao W, He C, Wang F Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Effect of nanohydroxyapatite on surface mineralization in acid-etched dentinal tubules and adsorption of lead ions].
Abstract OBJECTIVE: To study the effect of nanohydroxyapatite (nHA) for promoting surface mineralization of demineralized dentin discs and adsorption of lead ions in simulated sewage water. METHODS: Sixty dentin disks were prepared from freshly extracted teeth with intact crown (including 30 premolars and 30 molars) and treated with 10% citric acid for 2 min to simulate dentinal tubules with dentin hypersensitivity. The etched dentin discs were brushed with distilled water, 0.2 g HA or 0.2 g nHA for 2 min twice a day for 7 consecutive days, after which scanning electron microscopy (SEM) was performed and calc...
Source: Journal of Southern Medical University - September 29, 2020 Category: Universities & Medical Training Authors: Yang J, Yuan P, Liu C, Liu P, Ning H, Xu P Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Mir-29c-3p targeting TUG1 affects migration and invasion of bladder cancer cells by regulating CAPN7 expression].
Abstract OBJECTIVE: To investigate the mechanism by which long non-coding RNA TUG1 affects bladder cancer cell migration and invasion. METHODS: The expressions of TUG1 and miR-29c-3p were examined by quantitative RT-PCR (qRT-PCR) in 10 bladder cancer tissues and 5 bladder cancer cell lines. Trans-well assay was used to detect the changes in migration and invasion abilities of bladder cancer T24 cells after TUG1 knockdown using RNA interference technique, and the alteration in the expression of CAPN7 was also detected. The expression of CAPN7 was examined in T24 cells overexpressing mir-29c-3p by Western blott...
Source: Journal of Southern Medical University - September 29, 2020 Category: Universities & Medical Training Authors: Yu G, Zhou H, Xu K, Meng L, Lang B Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

"Nan Fang Yi Ke Da Xue Xue Bao"[ta]; +117 new citations
117 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: "Nan Fang Yi Ke Da Xue Xue Bao"[ta] These pubmed results were generated on 2020/09/10PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - September 10, 2020 Category: Universities & Medical Training Tags: Report Source Type: research

[Fluoxetine enhances combined learning and memory abilities of mice by promoting neural cell functions in the barrel cortex].
Abstract OBJECTIVE: To study the behavioral characteristics of memory maintenance and regression in a mouse model of combined learning and memory training with fluoxetine treatment and explore the neural basis for learning and memory in the barrel area of the brain. METHODS: Twenty-six 16-day-old C57 mice were randomized into two equal groups and were given daily intraperitoneal injection of saline (control) or fluoxetine. The mice were subjected to stimulation of the right whiskers using a multi-sensory stimulation simulator and were given simultaneously olfactory stimulation with butyl acetate. In the initi...
Source: Journal of Southern Medical University - March 29, 2020 Category: Universities & Medical Training Authors: Zhang T, Zhang W, Sun Y, Huo Q Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Effect of heat inactivation of blood samples on the efficacy of three detection methods of SARS-CoV-2 antibodies].
Abstract OBJECTIVE: To evaluate the effects of heat inactivation of blood samples at 56℃ for 30 min on the results of SARS-CoV-2 antibody detection using different methods. METHODS: This retrospective study was conducted in 11 patients with established diagnosis of COVID-19 and 10 patients with diseases other than COVID- 19 in our hospital. We collected samples of serum, plasma and whole blood from each patient between February, 12 and 18, 2020, and with a double- blind design, the samples were examined for SARS-CoV-2 antibodies before and after heat inactivation at 56 ℃ for 30 min. In all the samples, th...
Source: Journal of Southern Medical University - March 29, 2020 Category: Universities & Medical Training Authors: Xue X, Zhu C, Huang S, Pan L, Xu J, Li W Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Accuracy of two thyroid imaging, reporting and data systems for differential diagnosis of benign and malignant thyroid nodules].
CONCLUSIONS: Both ACR TI-RADS and Kwak TI-RADS have good performance for differential diagnosis of thyroid nodules, but ACR TI-RADS has a higher specificity and a lower sensitivity compared with Kwak TI-RADS. PMID: 32376572 [PubMed - in process] (Source: Journal of Southern Medical University)
Source: Journal of Southern Medical University - March 29, 2020 Category: Universities & Medical Training Authors: Lai S, Chen Y, Chen Z, Wang L, Cong S, Kuang J Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research

[Correlation between clinical classification of COVID-19 and imaging characteristics of MSCT volume scanning of the lungs].
Abstract OBJECTIVE: To investigate the correlation between the clinical classification of coronavirus disease 2019 (COVID-19) and the imaging characteristics of multislice spiral computed tomography (MSCT) volume scanning of the lungs. METHODS: The clinical data and thoracic MSCT volume scanning data were analyzed retrospectively for 102 patients with COVID-19 diagnosed and hospitalized in the First Affiliated Hospital of Bengbu Medical College between January 18 and February 26, 2020. According to the Fifth Edition of the Diagnosis and Treatment Guidelines by the National Health Commission, the patients were...
Source: Journal of Southern Medical University - March 29, 2020 Category: Universities & Medical Training Authors: Guo F, Zhu L, Xu H, Qin L, Liang X, Deng X Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research