Relapse of ankylosing spondylitis and its predictors after withdrawal of tumor necrosis factor- α inhibitors: a 52-week follow-up study
This study was conducted among 63 patients with AS who reduced the dose of or discontinued TNFi after completing at least 12 treatment cycles with ADA (40 mg/2 weeks) to achieve ASAS20 improvement with a BATH disease activity index (BASDAI)
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: C Tang F Chen S Zheng L Wu S Chen J Zhu J Li Source Type: research

Preparation of doxorubicin-loaded metallic organic nanoparticles and their effect for enhancing efficacy of high-intensity focused ultrasound therapy in tumor-bearing mice
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):640-648. doi: 10.12122/j.issn.1673-4254.2021.05.02.ABSTRACTOBJECTIVE: To prepare metallic organic nanoparticles that produce synergistic effect in high-intensity focused ultrasound (HIFU) therapy of tumors.OBJECTIVE: Glucose oxidase (GOD), MnO2, ferric iron (Fe3+) and doxorubicin (DOX) were self-assembled by physical adsorption with previously prepared manganese dioxide (MnO2) nanoparticles to obtain GOD-MnO2-Fe3+-DOX nanoparticles (GMFD NPs). HepG2 tumor-bearing nude mouse models were given intravenous injections of normal saline or GMFD NPs followed 4 h later by HIFU at th...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: H Ye N Huang T Sun W Hou J Bai H Li Source Type: research

< em > Escherichia coli < /em > expressing gas vesicles is safe for enhancing the ablation effect of highintensity focused ultrasound in tumor-bearing mice
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):649-656. doi: 10.12122/j.issn.1673-4254.2021.05.03.ABSTRACTOBJECTIVE: To investigate the effect and safety of Escherichia coli (E.coli) expressing gas vesicle (GVs) for enhancing the efficacy of tumor ablation by high intensity focused ultrasound (HIFU) in tumor-bearing mice.OBJECTIVE: Thirty-two female BALB/c mice were used to establish mouse models bearing 4T1 tumor, which were randomized into GVs group [E.coli BL21 (AI)-PET28a-Arg1] and control group (PBS), and the efficacy of HIFU ablation was evaluated by examining coagulative necrotic volume and pathology of the tumors...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: F Jiang H Yang L Wang Y Wang Y Tang D Wang Q Wang J Zou Source Type: research

Inhibiting autophagy by silencing ATG5 and ATG7 enhances inhibitory effect of DDP on DDP-resistant I-10 testicular cancer cells
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):657-663. doi: 10.12122/j.issn.1673-4254.2021.05.04.ABSTRACTOBJECTIVE: To observe the changes in autophagy of cisplatin-resistant I-10 testicular cancer cells (I-10/DDP cells) in response to cisplatin treatment and the effect of silencing ATG5 and ATG7 on autophagy and proliferation of cisplatin-treated cells.OBJECTIVE: I-10/DDP cells treated with 15 μmol/L cisplatin for 12 h were examined for expressions of LC3 and p62 by Western blotting and for autophagy level through transmission electron microscopy and mCherry-GFP-LC3B. I-10/DDP cells were transfected with short hairp...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: C Zhu J DU Y Yao D Wu M Yuan L Gan X Tong Source Type: research

rCsHscB derived from < em > Clonorchis < /em > < em > sinensis < /em > has therapeutic effect on dextran sodium sulfate-induced chronic ulcerative colitis in mice
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):664-670. doi: 10.12122/j.issn.1673-4254.2021.05.05.ABSTRACTOBJECTIVE: To investigate the therapeutic effect of rCsHscB derived from Clonorchis sinensis on dextran sodium sulfate (DSS)-induced chronic ulcerative colitis in mice.OBJECTIVE: C57BL/6 mice were randomized into negative control (NC) group (n= 10), rCsHscB group (n=10), DSS group (n=15), and DSS+rCsHscB group (n=15), and in the latter two groups, chronic ulcerative colitis was induced in the mice using 2% DSS. In rCsHscB and DSS+ rCsHscB groups, the mice received intraperitoneal injections of 125 μg/mL rCsHscB on...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: H Hua X Dong Y Zhang F Fang B Zhang X Li Q Yu K Zheng C Yan Source Type: research

Betelnut polyphenols provide protection against high-altitude hypoxia in rats
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):671-678. doi: 10.12122/j.issn.1673-4254.2021.05.06.ABSTRACTOBJECTIVE: To investigate the protective effects of betelnut polyphenols on the vital organs against high-altitude hypoxia in rats.OBJECTIVE: We compared low-, medium-, and high- dose betelnut polyphenols (400, 800, and 1600 mg/kg, respectively) and rhodiola the effects of against high-altitude hypoxia in Wistar rats. The rats were kept in normal condition and given the drugs daily for 3 days before transfer to a facility at the altitude of 4010 m, where the rats were kept for 5 consecutive days for hypoxic exposure....
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: Y Huo A Zhao J Song J Li R Wang Source Type: research

DJ-1 alleviates oxidative stress injury by activating the Nrf2 pathway in rats with cerebral ischemia-reperfusion injury
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):679-686. doi: 10.12122/j.issn.1673-4254.2021.05.07.ABSTRACTOBJECTIVE: To investigate the antioxidant effect of DJ-1 (Park7) in rats with cerebral ischemia/reperfusion (IR) injury and its potential mechanism.OBJECTIVE: A total of 108 SD rats were randomly divided into sham-operated group, middle cerebral artery occlusion (MCAO) group, Scramble group, DJ-1 siRNA group, negative control (NC) group and DJ-1 overexpression group. Except for those in the sham group, all the rats were subjected to MCAO to establish models of cerebral IR injury. In DJ-1 siRNA and DJ-1 overexpression...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: L Li L Peng J Zhu J Wu Y Zhao Source Type: research

Curcumol inhibits keloid fibroblast proliferation and collagen synthesis through the ERK signaling pathway
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):687-693. doi: 10.12122/j.issn.1673-4254.2021.05.08.ABSTRACTOBJECTIVE: To assess the effects of curcumol on the proliferation, apoptosis and collagen synthesis of keloid fibroblasts and explore the underlying mechanism.OBJECTIVE: Keloid fibroblasts were treated with different concentrations of curcumol (10, 20, 40, 80 and 160 mg/L) or with 160 mg/L curcumol and 20 μmol/L ISO (an ERK signaling pathway activator). Western blotting was performed to detect the expression levels of proliferation-associated proteins (cyclin D1 and PCNA), fibrosis marker proteins (Col1A1, Col3A1 ...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: W Yuan H Sun L Yu J Wang Source Type: research

Orexin-A inhibits γ-aminobutyric acid current of neonatal rat spinal cord ventral horn neurons by activating OX < sub > 1 < /sub > R, OX < sub > 2 < /sub > R and Ca < sup > 2+ < /sup > -independent PKC
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):694-701. doi: 10.12122/j.issn.1673-4254.2021.05.09.ABSTRACTOBJECTIVE: To investigate the effect of orexin-A on the functionality of ionotropic γ-aminobutyric acid (GABA) receptors in spinal cord ventral horn neurons and its mechanisms.OBJECTIVE: The spinal cord containing the lumbosacral enlargement was isolated from neonatal SD rats (7-12 days old) and sliced. The slices were digested with papain (in 0.18 g/30 mL artificial cerebrospinal fluid) for 40-60 min, and the ventral horn neurons were separated acutely using fire-polished Pasteur pipettes. After the cells adhe...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: X Yang S Zhu N Jin Y Li C Zhen H Zhang A Xu M Wang C Zheng Source Type: research

Heat stress induces neuronal apoptosis by up-regulating endoplasmic reticulum stress pathway
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):702-709. doi: 10.12122/j.issn.1673-4254.2021.05.10.ABSTRACTOBJECTIVE: To explore the role of endoplasmic reticulum stress in heat stress-induced apoptosis of human neuroblastoma SH-SY5Y cells.OBJECTIVE: SH-SY5Y cells were incubated at 43 ℃ for 2 h followed by further culture at 37 ℃ for 0, 3 h, or 6 h. With the cells cultured at 37 ℃ as the control, the cells exposed to heat stress were examined for morphological changes under optical microscope and changes in cell viability using CCK-8 assay. Flow cytometry was performed for detecting apoptosis of the cells following ...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: J Gao Y Liu J Ji Z Liu Source Type: research

Correlation of serum ADAMTS13 and TSP1 levels with myocardial injury and prognosis in patients with acute coronary syndrome
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):710-715. doi: 10.12122/j.issn.1673-4254.2021.05.11.ABSTRACTOBJECTIVE: To investigate serum levels of von Willebrand factor lytic protease (ADAMTS13) and thrombospondin-1 (TSP1) in patients with different types of acute coronary syndrome (ACS) and their correlation with the patients' clinical prognosis.OBJECTIVE: According to their disease history, results of angiography and clinical biochemical tests, a total of 405 patients undergoing coronary angiography, were divided into unstable angina (UAP) group (n=215), acute myocardial infarction (AMI) group (n=96), and angiographic...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: Z Yao B Bao S Qian Z Li Q Lu S Min M Li H Wang Source Type: research

Aryl hydrocarbon receptor modulates airway inflammation in mice with cockroach allergen-induced asthma by regulating Th17/Treg differentiation
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):716-721. doi: 10.12122/j.issn.1673-4254.2021.05.12.ABSTRACTOBJECTIVE: To investigate whether aryl hydrocarbon receptor (AhR) modulates cockroach allergen (CRE)-induced asthma by regulating Th17/Treg differentiation.OBJECTIVE: Mouse models of CRE-induced asthma established by sensitizing and challenging the mice with CRE were randomized into asthma model group, AhR agonist group treated with TCDD (10 μg/ kg), and AhR antagonist group treated with TCDD and CH223191 (10 mg/kg) (n=5), with 5 mice without CRE challenge as the control group. The expressions of AhR, Cyp1a1 and C...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: T Xu Z Cui J Wang Y Feng R Xie D Li J Peng R Huang T Li Source Type: research

Curcumin induces human lens epithelial cell apoptosis and cell cycle arrest by inhibiting Wnt/ β-catenin signaling pathway
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):722-728. doi: 10.12122/j.issn.1673-4254.2021.05.13.ABSTRACTOBJECTIVE: To investigate the effect of curcumin on cell cycle and apoptosis of human lens epithelial cells and the possible molecular mechanism.OBJECTIVE: Cultured human lens epithelial cell line HLEC-SRA01/04 was treated with 20, 40 and 60 μmol/L curcumin for 24 or 48 h. The cell proliferation inhibition rate was determined using MTT assay, and the changes in cell cycle, mitochondrial membrane potential and apoptosis rate were analyzed with flow cytometry. Western blotting was used to detect the expression level...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: M Zhu S Cui Z Hao W Wang Q Yang C Chen J Wang Q Zhou Source Type: research

Luteolin has a significant protective effect against cadmium-induced injury in lung epithelial Beas-2B cells
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):729-735. doi: 10.12122/j.issn.1673-4254.2021.05.14.ABSTRACTOBJECTIVE: To investigate the protective effect of luteolin against cadmium (Cd)-induced injury in human lung epithelial Beas-2B cells.OBJECTIVE: Beas-2B cells were treated with different concentrations of luteolin (0-160 μmol/L) or Cd (0-40 μmol/L) for 24 h, and the cell viability was examined using MTT assay. After treatment with luteolin (0.25, 0.5 and 0.75 μmol/L) with or without Cd (5 μmol/L) for 24 h, the cells were examined for viability, lactate dehydrogenase (LDH) activity and morphological chang...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: N Chu X Zhang S Chen Q Zhen Y Wang Source Type: research

Development and functional validation of a nano-delivery system of miR-16/polypeptide targeting ovarian cancer cells
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):736-746. doi: 10.12122/j.issn.1673-4254.2021.05.15.ABSTRACTOBJECTIVE: To develop a nano-delivery system for targeted delivery of miR-16/polypeptide for enhancing cisplatin sensitivity of ovarian cancer.OBJECTIVE: R9-SS-R9 and cRGD-R9-SS-R9 peptides were synthesized and self-assembled with miR-16 molecules to form a nano-delivery system. The stability, particle size, potential and morphology of the nanoparticles were determined by agarose gel electrophoresis, particle size potentiometer and transmission electron microscopy. CCK-8 assay was used to assess the toxicity of the p...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: Q Li B Cen W Huang J Chen Z Chen J Pang W Fu S He A Ji Source Type: research

Effect of fatty acid synthase gene silencing on lipid metabolism and biological behaviors of human hepatoblastoma HepG2 cells
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):747-753. doi: 10.12122/j.issn.1673-4254.2021.05.16.ABSTRACTOBJECTIVE: To investigate the effect of fatty acid synthase (FASN) gene silencing on lipid metabolism and biological behaviors of human hepatoblastoma HepG2 cells.OBJECTIVE: Small interfering RNA (siRNA) targeting FASN gene or a negative control siRNA sequence (NC-siRNA) was transfected into HepG2 cells, and the gene silencing efficiency was evaluated with qRT-PCR and Western blotting. Triglyceride level in the cells was detected using enzyme-linked immunosorbent assay, and Oil red O staining was performed to examine...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: H Huang Y Wei J Wang F Ran Q Chen Source Type: research

Anatomy of the deep circumflex iliac artery perforators and reconstruction of complex mandibular defects with chimeric deep circumflex iliac artery perforator flap
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):754-759. doi: 10.12122/j.issn.1673-4254.2021.05.17.ABSTRACTOBJECTIVE: To investigate the anatomy of the perforator vessels of the deep circumflex iliac artery (DCIA) and the techniques for repairing mandibular complex defect using chimeric deep circumflex iliac artery perforator flap (DCIAPF).OBJECTIVE: We analyzed the origin, distribution, number and courses of the perforator vessels of the DCIA, and measured the outside diameters of the vessels at the origin in 6 adult cadaveric specimens (12 sides) with latex perfusion. From July, 2018 to September, 2019, based on the res...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: Y Chen X Hong L Liu Z Zhan X Li C Pu S Chen S Liao Source Type: research

Effect of stable overexpression of XAF1 gene on biological characteristics of ovarian cancer A2780 cells
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):760-766. doi: 10.12122/j.issn.1673-4254.2021.05.18.ABSTRACTOBJECTIVE: To construct an ovarian cancer cell line stably overexpressing XAF1 gene and observe the effects of XAF1 gene overexpression on proliferation, apoptosis, cell cycle and sensitivity to paclitaxel of the cells.OBJECTIVE: Ovarian cancer A2780 cells were transfected with the plasmids pcDNA3.1(+) or pcDNA3.1(+)-XAF1, and the cells stably Over expressing XAF1 (A2780/XAF1 cells) were screened using G418. Cell clone formation assay and CCK8 assay were used to evaluate the changes in proliferation and paclitaxel se...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: J Liu X Liu B Wei J Liu Y Wang H Liu Source Type: research

Establishment of a 3D ultrasound imaging system based on pulse-triggered image acquisition
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):767-774. doi: 10.12122/j.issn.1673-4254.2021.05.19.ABSTRACTOBJECTIVE: To establish a 3D ultrasound imaging system based on pulse-triggered image acquisition using the linear probe on the VerasonicsTM vantage 128 platform and evaluate its performance in scanning standard phantom and human carotid artery.OBJECTIVE: The 3D ultrasound imaging system included 3 modules for probe motion control, image acquisition and storage, and 3D image reconstruction and display. To improve the precision of image acquisition, we used fixed frequency pulses to control the external trigger functi...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: C Xu G Li Q Huang H Yang Q Wang Q Feng Source Type: research

Platelet-rich plasma alleviates myocardial ischemia-reperfusion injury in rats
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):775-782. doi: 10.12122/j.issn.1673-4254.2021.05.20.ABSTRACTOBJECTIVE: To investigate the protective effect of platelet-rich plasma (PRP) against acute myocardial ischemiareperfusion (IR) injury and the possible mechanism.OBJECTIVE: Aortic blood samples were collected from 10 SD rats to prepare PRP, in which the concentrations of platelet-derived growth factor-BB (PDGF-BB) and transforming growth factor-β1 (TGF-β1) were measured. Cell models of IR injury were established in primary cultures of neonatal SD rat cardiomyocytes by exposing the cells to 3 h of hypoxia. T...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: D Wang T Li Y Xu X Yang M He Z Zhang W Wu Y Yan Source Type: research

Comparison of < sup > 1 < /sup > H-MRS, Dixon fat-water separation and Z-spectral imaging for quantification of brown adipose tissue in rats
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):783-788. doi: 10.12122/j.issn.1673-4254.2021.05.21.ABSTRACTOBJECTIVE: To evaluate the performance of 1H-magnetic resonance spectroscopy (1H-MRS), Dixon fat-water separation and Z-spectral magnetic resonance imaging (ZS-MRI) for quantification of fat content in phantoms and brown adipose tissues in rats.OBJECTIVE: First, six water-oil mixture phantoms with different fat fractions (0, 20%, 40%, 60%, 80% and 100%) were prepared and placed in a 50-mL centrifuge tube. ZS-MRI, 1H-MRS and Dixon's method were used to quantitatively evaluate the fat content of the phantom, and the re...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: Z Cai P Yi Q Tao Y Feng Source Type: research

A novel frameshift NDUFV1 mutation in a child with the phenotype of optic nerve atrophy
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):789-792. doi: 10.12122/j.issn.1673-4254.2021.05.22.ABSTRACTOBJECTIVE: To investigate the pathogenic gene in a child with optic atrophy and analyze the influence of this gene mutation on protein structure.OBJECTIVE: We collected the clinical record of the 13-year-old girl and her relatives. The child received examinations of the visual acuity, visual field, fundus, OCT, visual-evoked potential (VEP) and the nerve system, underwent brain MRI and was followed up for 1 year. Genomic DNA was extracted from the peripheral blood of the child and her parents for next-generation sequ...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: Z Zhang H Yuan S Zhang Source Type: research

Effect of extracorporeal membrane oxygenation on pharmacokinetics of antimicrobial drugs: recent progress and recommendations
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):793-800. doi: 10.12122/j.issn.1673-4254.2021.05.23.ABSTRACTExtracorporeal membrane oxygenation (ECMO) is an effective means to provide life support for patients with severe respiratory or heart failure. Existing studies have shown that ECMO may affect the metabolic process of some drugs by drug adsorption, increasing the apparent distribution volume and changing the clearance rate of the drugs. This review summarizes the recent progress in the studies of the effect of ECMO on the pharmacokinetics of antibacterial and antifungal drugs. For the antibacterial drugs, it is recom...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: Y Zhang Z Zeng Q Zhang Q Ou Z Chen Source Type: research

Relapse of ankylosing spondylitis and its predictors after withdrawal of tumor necrosis factor- α inhibitors: a 52-week follow-up study
This study was conducted among 63 patients with AS who reduced the dose of or discontinued TNFi after completing at least 12 treatment cycles with ADA (40 mg/2 weeks) to achieve ASAS20 improvement with a BATH disease activity index (BASDAI)
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: C Tang F Chen S Zheng L Wu S Chen J Zhu J Li Source Type: research

Preparation of doxorubicin-loaded metallic organic nanoparticles and their effect for enhancing efficacy of high-intensity focused ultrasound therapy in tumor-bearing mice
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):640-648. doi: 10.12122/j.issn.1673-4254.2021.05.02.ABSTRACTOBJECTIVE: To prepare metallic organic nanoparticles that produce synergistic effect in high-intensity focused ultrasound (HIFU) therapy of tumors.OBJECTIVE: Glucose oxidase (GOD), MnO2, ferric iron (Fe3+) and doxorubicin (DOX) were self-assembled by physical adsorption with previously prepared manganese dioxide (MnO2) nanoparticles to obtain GOD-MnO2-Fe3+-DOX nanoparticles (GMFD NPs). HepG2 tumor-bearing nude mouse models were given intravenous injections of normal saline or GMFD NPs followed 4 h later by HIFU at th...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: H Ye N Huang T Sun W Hou J Bai H Li Source Type: research

< em > Escherichia coli < /em > expressing gas vesicles is safe for enhancing the ablation effect of highintensity focused ultrasound in tumor-bearing mice
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):649-656. doi: 10.12122/j.issn.1673-4254.2021.05.03.ABSTRACTOBJECTIVE: To investigate the effect and safety of Escherichia coli (E.coli) expressing gas vesicle (GVs) for enhancing the efficacy of tumor ablation by high intensity focused ultrasound (HIFU) in tumor-bearing mice.OBJECTIVE: Thirty-two female BALB/c mice were used to establish mouse models bearing 4T1 tumor, which were randomized into GVs group [E.coli BL21 (AI)-PET28a-Arg1] and control group (PBS), and the efficacy of HIFU ablation was evaluated by examining coagulative necrotic volume and pathology of the tumors...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: F Jiang H Yang L Wang Y Wang Y Tang D Wang Q Wang J Zou Source Type: research

Inhibiting autophagy by silencing ATG5 and ATG7 enhances inhibitory effect of DDP on DDP-resistant I-10 testicular cancer cells
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):657-663. doi: 10.12122/j.issn.1673-4254.2021.05.04.ABSTRACTOBJECTIVE: To observe the changes in autophagy of cisplatin-resistant I-10 testicular cancer cells (I-10/DDP cells) in response to cisplatin treatment and the effect of silencing ATG5 and ATG7 on autophagy and proliferation of cisplatin-treated cells.OBJECTIVE: I-10/DDP cells treated with 15 μmol/L cisplatin for 12 h were examined for expressions of LC3 and p62 by Western blotting and for autophagy level through transmission electron microscopy and mCherry-GFP-LC3B. I-10/DDP cells were transfected with short hairp...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: C Zhu J DU Y Yao D Wu M Yuan L Gan X Tong Source Type: research

rCsHscB derived from < em > Clonorchis < /em > < em > sinensis < /em > has therapeutic effect on dextran sodium sulfate-induced chronic ulcerative colitis in mice
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):664-670. doi: 10.12122/j.issn.1673-4254.2021.05.05.ABSTRACTOBJECTIVE: To investigate the therapeutic effect of rCsHscB derived from Clonorchis sinensis on dextran sodium sulfate (DSS)-induced chronic ulcerative colitis in mice.OBJECTIVE: C57BL/6 mice were randomized into negative control (NC) group (n= 10), rCsHscB group (n=10), DSS group (n=15), and DSS+rCsHscB group (n=15), and in the latter two groups, chronic ulcerative colitis was induced in the mice using 2% DSS. In rCsHscB and DSS+ rCsHscB groups, the mice received intraperitoneal injections of 125 μg/mL rCsHscB on...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: H Hua X Dong Y Zhang F Fang B Zhang X Li Q Yu K Zheng C Yan Source Type: research

Betelnut polyphenols provide protection against high-altitude hypoxia in rats
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):671-678. doi: 10.12122/j.issn.1673-4254.2021.05.06.ABSTRACTOBJECTIVE: To investigate the protective effects of betelnut polyphenols on the vital organs against high-altitude hypoxia in rats.OBJECTIVE: We compared low-, medium-, and high- dose betelnut polyphenols (400, 800, and 1600 mg/kg, respectively) and rhodiola the effects of against high-altitude hypoxia in Wistar rats. The rats were kept in normal condition and given the drugs daily for 3 days before transfer to a facility at the altitude of 4010 m, where the rats were kept for 5 consecutive days for hypoxic exposure....
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: Y Huo A Zhao J Song J Li R Wang Source Type: research

DJ-1 alleviates oxidative stress injury by activating the Nrf2 pathway in rats with cerebral ischemia-reperfusion injury
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):679-686. doi: 10.12122/j.issn.1673-4254.2021.05.07.ABSTRACTOBJECTIVE: To investigate the antioxidant effect of DJ-1 (Park7) in rats with cerebral ischemia/reperfusion (IR) injury and its potential mechanism.OBJECTIVE: A total of 108 SD rats were randomly divided into sham-operated group, middle cerebral artery occlusion (MCAO) group, Scramble group, DJ-1 siRNA group, negative control (NC) group and DJ-1 overexpression group. Except for those in the sham group, all the rats were subjected to MCAO to establish models of cerebral IR injury. In DJ-1 siRNA and DJ-1 overexpression...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: L Li L Peng J Zhu J Wu Y Zhao Source Type: research

Curcumol inhibits keloid fibroblast proliferation and collagen synthesis through the ERK signaling pathway
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):687-693. doi: 10.12122/j.issn.1673-4254.2021.05.08.ABSTRACTOBJECTIVE: To assess the effects of curcumol on the proliferation, apoptosis and collagen synthesis of keloid fibroblasts and explore the underlying mechanism.OBJECTIVE: Keloid fibroblasts were treated with different concentrations of curcumol (10, 20, 40, 80 and 160 mg/L) or with 160 mg/L curcumol and 20 μmol/L ISO (an ERK signaling pathway activator). Western blotting was performed to detect the expression levels of proliferation-associated proteins (cyclin D1 and PCNA), fibrosis marker proteins (Col1A1, Col3A1 ...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: W Yuan H Sun L Yu J Wang Source Type: research

Orexin-A inhibits γ-aminobutyric acid current of neonatal rat spinal cord ventral horn neurons by activating OX < sub > 1 < /sub > R, OX < sub > 2 < /sub > R and Ca < sup > 2+ < /sup > -independent PKC
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):694-701. doi: 10.12122/j.issn.1673-4254.2021.05.09.ABSTRACTOBJECTIVE: To investigate the effect of orexin-A on the functionality of ionotropic γ-aminobutyric acid (GABA) receptors in spinal cord ventral horn neurons and its mechanisms.OBJECTIVE: The spinal cord containing the lumbosacral enlargement was isolated from neonatal SD rats (7-12 days old) and sliced. The slices were digested with papain (in 0.18 g/30 mL artificial cerebrospinal fluid) for 40-60 min, and the ventral horn neurons were separated acutely using fire-polished Pasteur pipettes. After the cells adhe...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: X Yang S Zhu N Jin Y Li C Zhen H Zhang A Xu M Wang C Zheng Source Type: research

Heat stress induces neuronal apoptosis by up-regulating endoplasmic reticulum stress pathway
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):702-709. doi: 10.12122/j.issn.1673-4254.2021.05.10.ABSTRACTOBJECTIVE: To explore the role of endoplasmic reticulum stress in heat stress-induced apoptosis of human neuroblastoma SH-SY5Y cells.OBJECTIVE: SH-SY5Y cells were incubated at 43 ℃ for 2 h followed by further culture at 37 ℃ for 0, 3 h, or 6 h. With the cells cultured at 37 ℃ as the control, the cells exposed to heat stress were examined for morphological changes under optical microscope and changes in cell viability using CCK-8 assay. Flow cytometry was performed for detecting apoptosis of the cells following ...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: J Gao Y Liu J Ji Z Liu Source Type: research

Correlation of serum ADAMTS13 and TSP1 levels with myocardial injury and prognosis in patients with acute coronary syndrome
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):710-715. doi: 10.12122/j.issn.1673-4254.2021.05.11.ABSTRACTOBJECTIVE: To investigate serum levels of von Willebrand factor lytic protease (ADAMTS13) and thrombospondin-1 (TSP1) in patients with different types of acute coronary syndrome (ACS) and their correlation with the patients' clinical prognosis.OBJECTIVE: According to their disease history, results of angiography and clinical biochemical tests, a total of 405 patients undergoing coronary angiography, were divided into unstable angina (UAP) group (n=215), acute myocardial infarction (AMI) group (n=96), and angiographic...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: Z Yao B Bao S Qian Z Li Q Lu S Min M Li H Wang Source Type: research

Aryl hydrocarbon receptor modulates airway inflammation in mice with cockroach allergen-induced asthma by regulating Th17/Treg differentiation
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):716-721. doi: 10.12122/j.issn.1673-4254.2021.05.12.ABSTRACTOBJECTIVE: To investigate whether aryl hydrocarbon receptor (AhR) modulates cockroach allergen (CRE)-induced asthma by regulating Th17/Treg differentiation.OBJECTIVE: Mouse models of CRE-induced asthma established by sensitizing and challenging the mice with CRE were randomized into asthma model group, AhR agonist group treated with TCDD (10 μg/ kg), and AhR antagonist group treated with TCDD and CH223191 (10 mg/kg) (n=5), with 5 mice without CRE challenge as the control group. The expressions of AhR, Cyp1a1 and C...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: T Xu Z Cui J Wang Y Feng R Xie D Li J Peng R Huang T Li Source Type: research

Curcumin induces human lens epithelial cell apoptosis and cell cycle arrest by inhibiting Wnt/ β-catenin signaling pathway
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):722-728. doi: 10.12122/j.issn.1673-4254.2021.05.13.ABSTRACTOBJECTIVE: To investigate the effect of curcumin on cell cycle and apoptosis of human lens epithelial cells and the possible molecular mechanism.OBJECTIVE: Cultured human lens epithelial cell line HLEC-SRA01/04 was treated with 20, 40 and 60 μmol/L curcumin for 24 or 48 h. The cell proliferation inhibition rate was determined using MTT assay, and the changes in cell cycle, mitochondrial membrane potential and apoptosis rate were analyzed with flow cytometry. Western blotting was used to detect the expression level...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: M Zhu S Cui Z Hao W Wang Q Yang C Chen J Wang Q Zhou Source Type: research

Luteolin has a significant protective effect against cadmium-induced injury in lung epithelial Beas-2B cells
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):729-735. doi: 10.12122/j.issn.1673-4254.2021.05.14.ABSTRACTOBJECTIVE: To investigate the protective effect of luteolin against cadmium (Cd)-induced injury in human lung epithelial Beas-2B cells.OBJECTIVE: Beas-2B cells were treated with different concentrations of luteolin (0-160 μmol/L) or Cd (0-40 μmol/L) for 24 h, and the cell viability was examined using MTT assay. After treatment with luteolin (0.25, 0.5 and 0.75 μmol/L) with or without Cd (5 μmol/L) for 24 h, the cells were examined for viability, lactate dehydrogenase (LDH) activity and morphological chang...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: N Chu X Zhang S Chen Q Zhen Y Wang Source Type: research

Development and functional validation of a nano-delivery system of miR-16/polypeptide targeting ovarian cancer cells
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):736-746. doi: 10.12122/j.issn.1673-4254.2021.05.15.ABSTRACTOBJECTIVE: To develop a nano-delivery system for targeted delivery of miR-16/polypeptide for enhancing cisplatin sensitivity of ovarian cancer.OBJECTIVE: R9-SS-R9 and cRGD-R9-SS-R9 peptides were synthesized and self-assembled with miR-16 molecules to form a nano-delivery system. The stability, particle size, potential and morphology of the nanoparticles were determined by agarose gel electrophoresis, particle size potentiometer and transmission electron microscopy. CCK-8 assay was used to assess the toxicity of the p...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: Q Li B Cen W Huang J Chen Z Chen J Pang W Fu S He A Ji Source Type: research

Effect of fatty acid synthase gene silencing on lipid metabolism and biological behaviors of human hepatoblastoma HepG2 cells
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):747-753. doi: 10.12122/j.issn.1673-4254.2021.05.16.ABSTRACTOBJECTIVE: To investigate the effect of fatty acid synthase (FASN) gene silencing on lipid metabolism and biological behaviors of human hepatoblastoma HepG2 cells.OBJECTIVE: Small interfering RNA (siRNA) targeting FASN gene or a negative control siRNA sequence (NC-siRNA) was transfected into HepG2 cells, and the gene silencing efficiency was evaluated with qRT-PCR and Western blotting. Triglyceride level in the cells was detected using enzyme-linked immunosorbent assay, and Oil red O staining was performed to examine...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: H Huang Y Wei J Wang F Ran Q Chen Source Type: research

Anatomy of the deep circumflex iliac artery perforators and reconstruction of complex mandibular defects with chimeric deep circumflex iliac artery perforator flap
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):754-759. doi: 10.12122/j.issn.1673-4254.2021.05.17.ABSTRACTOBJECTIVE: To investigate the anatomy of the perforator vessels of the deep circumflex iliac artery (DCIA) and the techniques for repairing mandibular complex defect using chimeric deep circumflex iliac artery perforator flap (DCIAPF).OBJECTIVE: We analyzed the origin, distribution, number and courses of the perforator vessels of the DCIA, and measured the outside diameters of the vessels at the origin in 6 adult cadaveric specimens (12 sides) with latex perfusion. From July, 2018 to September, 2019, based on the res...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: Y Chen X Hong L Liu Z Zhan X Li C Pu S Chen S Liao Source Type: research

Effect of stable overexpression of XAF1 gene on biological characteristics of ovarian cancer A2780 cells
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):760-766. doi: 10.12122/j.issn.1673-4254.2021.05.18.ABSTRACTOBJECTIVE: To construct an ovarian cancer cell line stably overexpressing XAF1 gene and observe the effects of XAF1 gene overexpression on proliferation, apoptosis, cell cycle and sensitivity to paclitaxel of the cells.OBJECTIVE: Ovarian cancer A2780 cells were transfected with the plasmids pcDNA3.1(+) or pcDNA3.1(+)-XAF1, and the cells stably Over expressing XAF1 (A2780/XAF1 cells) were screened using G418. Cell clone formation assay and CCK8 assay were used to evaluate the changes in proliferation and paclitaxel se...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: J Liu X Liu B Wei J Liu Y Wang H Liu Source Type: research

Establishment of a 3D ultrasound imaging system based on pulse-triggered image acquisition
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):767-774. doi: 10.12122/j.issn.1673-4254.2021.05.19.ABSTRACTOBJECTIVE: To establish a 3D ultrasound imaging system based on pulse-triggered image acquisition using the linear probe on the VerasonicsTM vantage 128 platform and evaluate its performance in scanning standard phantom and human carotid artery.OBJECTIVE: The 3D ultrasound imaging system included 3 modules for probe motion control, image acquisition and storage, and 3D image reconstruction and display. To improve the precision of image acquisition, we used fixed frequency pulses to control the external trigger functi...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: C Xu G Li Q Huang H Yang Q Wang Q Feng Source Type: research

Platelet-rich plasma alleviates myocardial ischemia-reperfusion injury in rats
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):775-782. doi: 10.12122/j.issn.1673-4254.2021.05.20.ABSTRACTOBJECTIVE: To investigate the protective effect of platelet-rich plasma (PRP) against acute myocardial ischemiareperfusion (IR) injury and the possible mechanism.OBJECTIVE: Aortic blood samples were collected from 10 SD rats to prepare PRP, in which the concentrations of platelet-derived growth factor-BB (PDGF-BB) and transforming growth factor-β1 (TGF-β1) were measured. Cell models of IR injury were established in primary cultures of neonatal SD rat cardiomyocytes by exposing the cells to 3 h of hypoxia. T...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: D Wang T Li Y Xu X Yang M He Z Zhang W Wu Y Yan Source Type: research

Comparison of < sup > 1 < /sup > H-MRS, Dixon fat-water separation and Z-spectral imaging for quantification of brown adipose tissue in rats
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):783-788. doi: 10.12122/j.issn.1673-4254.2021.05.21.ABSTRACTOBJECTIVE: To evaluate the performance of 1H-magnetic resonance spectroscopy (1H-MRS), Dixon fat-water separation and Z-spectral magnetic resonance imaging (ZS-MRI) for quantification of fat content in phantoms and brown adipose tissues in rats.OBJECTIVE: First, six water-oil mixture phantoms with different fat fractions (0, 20%, 40%, 60%, 80% and 100%) were prepared and placed in a 50-mL centrifuge tube. ZS-MRI, 1H-MRS and Dixon's method were used to quantitatively evaluate the fat content of the phantom, and the re...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: Z Cai P Yi Q Tao Y Feng Source Type: research

A novel frameshift NDUFV1 mutation in a child with the phenotype of optic nerve atrophy
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):789-792. doi: 10.12122/j.issn.1673-4254.2021.05.22.ABSTRACTOBJECTIVE: To investigate the pathogenic gene in a child with optic atrophy and analyze the influence of this gene mutation on protein structure.OBJECTIVE: We collected the clinical record of the 13-year-old girl and her relatives. The child received examinations of the visual acuity, visual field, fundus, OCT, visual-evoked potential (VEP) and the nerve system, underwent brain MRI and was followed up for 1 year. Genomic DNA was extracted from the peripheral blood of the child and her parents for next-generation sequ...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: Z Zhang H Yuan S Zhang Source Type: research

Effect of extracorporeal membrane oxygenation on pharmacokinetics of antimicrobial drugs: recent progress and recommendations
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):793-800. doi: 10.12122/j.issn.1673-4254.2021.05.23.ABSTRACTExtracorporeal membrane oxygenation (ECMO) is an effective means to provide life support for patients with severe respiratory or heart failure. Existing studies have shown that ECMO may affect the metabolic process of some drugs by drug adsorption, increasing the apparent distribution volume and changing the clearance rate of the drugs. This review summarizes the recent progress in the studies of the effect of ECMO on the pharmacokinetics of antibacterial and antifungal drugs. For the antibacterial drugs, it is recom...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: Y Zhang Z Zeng Q Zhang Q Ou Z Chen Source Type: research

Relapse of ankylosing spondylitis and its predictors after withdrawal of tumor necrosis factor- α inhibitors: a 52-week follow-up study
This study was conducted among 63 patients with AS who reduced the dose of or discontinued TNFi after completing at least 12 treatment cycles with ADA (40 mg/2 weeks) to achieve ASAS20 improvement with a BATH disease activity index (BASDAI)
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: C Tang F Chen S Zheng L Wu S Chen J Zhu J Li Source Type: research

Preparation of doxorubicin-loaded metallic organic nanoparticles and their effect for enhancing efficacy of high-intensity focused ultrasound therapy in tumor-bearing mice
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):640-648. doi: 10.12122/j.issn.1673-4254.2021.05.02.ABSTRACTOBJECTIVE: To prepare metallic organic nanoparticles that produce synergistic effect in high-intensity focused ultrasound (HIFU) therapy of tumors.OBJECTIVE: Glucose oxidase (GOD), MnO2, ferric iron (Fe3+) and doxorubicin (DOX) were self-assembled by physical adsorption with previously prepared manganese dioxide (MnO2) nanoparticles to obtain GOD-MnO2-Fe3+-DOX nanoparticles (GMFD NPs). HepG2 tumor-bearing nude mouse models were given intravenous injections of normal saline or GMFD NPs followed 4 h later by HIFU at th...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: H Ye N Huang T Sun W Hou J Bai H Li Source Type: research

< em > Escherichia coli < /em > expressing gas vesicles is safe for enhancing the ablation effect of highintensity focused ultrasound in tumor-bearing mice
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):649-656. doi: 10.12122/j.issn.1673-4254.2021.05.03.ABSTRACTOBJECTIVE: To investigate the effect and safety of Escherichia coli (E.coli) expressing gas vesicle (GVs) for enhancing the efficacy of tumor ablation by high intensity focused ultrasound (HIFU) in tumor-bearing mice.OBJECTIVE: Thirty-two female BALB/c mice were used to establish mouse models bearing 4T1 tumor, which were randomized into GVs group [E.coli BL21 (AI)-PET28a-Arg1] and control group (PBS), and the efficacy of HIFU ablation was evaluated by examining coagulative necrotic volume and pathology of the tumors...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: F Jiang H Yang L Wang Y Wang Y Tang D Wang Q Wang J Zou Source Type: research

Inhibiting autophagy by silencing ATG5 and ATG7 enhances inhibitory effect of DDP on DDP-resistant I-10 testicular cancer cells
Nan Fang Yi Ke Da Xue Xue Bao. 2021 May 20;41(5):657-663. doi: 10.12122/j.issn.1673-4254.2021.05.04.ABSTRACTOBJECTIVE: To observe the changes in autophagy of cisplatin-resistant I-10 testicular cancer cells (I-10/DDP cells) in response to cisplatin treatment and the effect of silencing ATG5 and ATG7 on autophagy and proliferation of cisplatin-treated cells.OBJECTIVE: I-10/DDP cells treated with 15 μmol/L cisplatin for 12 h were examined for expressions of LC3 and p62 by Western blotting and for autophagy level through transmission electron microscopy and mCherry-GFP-LC3B. I-10/DDP cells were transfected with short hairp...
Source: Journal of Southern Medical University - June 17, 2021 Category: Universities & Medical Training Authors: C Zhu J DU Y Yao D Wu M Yuan L Gan X Tong Source Type: research