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HDAC inhibitor chidamide synergizes with venetoclax to inhibit the growth of diffuse large B-cell lymphoma via down-regulation of MYC, BCL2, and TP53 expression
In this study the synergistic mechanism of chidamide and venetoclax in DLBCL was determined through in vitro and in vivo models. We found that combination therapy significantly reduced the protein levels of MYC TP53 and BCL2 in activated apoptotic-related pathways in DLBCL cells by increasing BIM levels and inducing cell apoptosis. Moreover combination therapy regulated expression of multiple transcriptomes in DLBCL cells involving apoptosis cell cycle phosphorylation and other biological processes and significantly inhibited tumor growth in DLBCL-bearing xenograft mice. Taken together these findings verify the in vivo the...
Source: Journal of Zhejiang University. Science. B. - August 1, 2022 Category: Universities & Medical Training Source Type: research

PFKL, a novel regulatory node for NOX2-dependent oxidative burst and NETosis
概要中性粒细胞胞外诱捕网(NETs), 由活化的中性粒细胞释放的组蛋白和抗菌蛋白修饰的网状DNA纤维组成。中性粒细胞产生NETs, 实现宿主防御作用, 这一过程被称为中性粒细胞炎性死亡(NETosis)。NETosis是一把双刃剑, 在应对各种外界刺激时 , NETosis介导免疫反应, 捕获和清除病原体, 促进中性粒细胞和吞噬细胞的吞噬作用。然而, 过度的NETosis增加炎症反应, 持续炎症状态。因此, 抑制异常NETosis的发生可以降低各种自身免疫性疾病和炎症性疾病的严重程度。磷酸果糖激 酶L(PFKL)通过选...
Source: Journal of Zhejiang University. Science. B. - July 1, 2022 Category: Universities & Medical Training Source Type: research

ZNF750 facilitates carcinogenesis via promoting the expression of long non-coding RNA CYTOR and influences pharmacotherapy response in colon adenocarcinoma
Abstract目的探讨锌指蛋白750在调控长链非编码RNACYTOR表达进而影响结直肠癌发生发展中的相关表型和机制及其影响药物应答和预后的潜在临床意义。创新点聚焦锌指蛋白750在结肠腺癌中的促癌机制, 并在其对CYTOR表达的影响及锌指蛋白750-CYTOR与结肠腺癌临床相关分子病理特征和药物疗效预测方面展开研究。方法在分子水平, 利用蛋白质免疫印迹(western blotting),染色质免疫沉淀-定量聚合酶链反应(ChIP-qPCR),转录组测序(RNA-seq),逆转录-定量聚合酶链反应(RT-qPCR)等技术手段对ZNF750和CYTOR...
Source: Journal of Zhejiang University. Science. B. - July 1, 2022 Category: Universities & Medical Training Source Type: research

Bioactive constituents of animal-derived traditional Chinese medicinal materials for breast cancer: opportunities and challenges
AbstractBreast cancer is globally the most common invasive cancer in women and remains one of the leading causes of cancer-related deaths. Surgery, radiotherapy, chemotherapy, immunotherapy, and endocrine therapy are currently the main treatments for this cancer type. However, some breast cancer patients are prone to drug resistance related to chemotherapy or immunotherapy, resulting in limited treatment efficacy. Consequently, traditional Chinese medicinal materials (TCMMs) as natural products have become an attractive source of novel drugs. In this review, we summarized the current knowledge on the active components of a...
Source: Journal of Zhejiang University. Science. B. - July 1, 2022 Category: Universities & Medical Training Source Type: research

Detection, isolation, and characterization of a novel impurity from several folic acid products
概要叶酸是一种重要的营养物质, 常用于孕妇和婴幼儿人群的食品强化和营养补充。在叶酸产品的生产和储运过程中, 常会产生一些杂质, 其中大部分已获得结构鉴定, 其中一些已被收录入药典。本研究报道了一种新的叶酸杂质K, 该杂质 存在于多种叶酸产品中。本研究采用制备型高效液相色谱对该杂质进行了成功分离, 并采用多维核磁共振谱、高分辨质谱、傅里叶红外光谱和紫外光谱等分析手段, 对其化学结构进行了鉴定。结果显示, 该杂质是药典记载的叶酸杂 质D的同分异构...
Source: Journal of Zhejiang University. Science. B. - July 1, 2022 Category: Universities & Medical Training Source Type: research

Novel apical-to-femoral rail technique for horizontal aorta in transcatheter aortic valve replacement
Abstract目的为经导管主动脉瓣置换术中治疗极度横位心的主动脉瓣狭窄患者制定手术策略提供借鉴。创新点本研究的患者为老年女性, 她被诊断为重度主动脉瓣狭窄, 拟接受经导管主动脉瓣置换术。术前电子计算机断层扫描(CT)结果显示患者为主动脉瓣呈type 0型二叶式主动脉瓣, 主动脉成角为84°, 为极度横位心。术中我们通过建立心尖-股 动脉轨道实现瓣膜同轴置入, 术后病人无并发症发生。方法在左胸前外侧第五肋间切一小口, 打开心包, 经心尖引入圈套器, 捕捉经右侧股动脉...
Source: Journal of Zhejiang University. Science. B. - July 1, 2022 Category: Universities & Medical Training Source Type: research

Co-regulation of circadian clock genes and microRNAs in bone metabolism
AbstractMammalian bone is constantly metabolized from the embryonic stage, and the maintenance of bone health depends on the dynamic balance between bone resorption and bone formation, mediated by osteoclasts and osteoblasts. It is widely recognized that circadian clock genes can regulate bone metabolism. In recent years, the regulation of bone metabolism by non-coding RNAs has become a hotspot of research. MicroRNAs can participate in bone catabolism and anabolism by targeting key factors related to bone metabolism, including circadian clock genes. However, research in this field has been conducted only in recent years an...
Source: Journal of Zhejiang University. Science. B. - July 1, 2022 Category: Universities & Medical Training Source Type: research

High-throughput “read-on-ski” automated imaging and label-free detection system for toxicity screening of compounds using personalised human kidney organoids
AbstractOrganoid models are used to study kidney physiology, such as the assessment of nephrotoxicity and underlying disease processes. Personalized human pluripotent stem cell-derived kidney organoids are ideal models for compound toxicity studies, but there is a need to accelerate basic and translational research in the field. Here, we developed an automated continuous imaging setup with the “read-on-ski” law of control to maximize temporal resolution with minimum culture plate vibration. High-accuracy performance was achieved: organoid screening and imaging were performed at a spatial resolution of 1.1 µm for the e...
Source: Journal of Zhejiang University. Science. B. - July 1, 2022 Category: Universities & Medical Training Source Type: research

Assessment of quality deviation of pork and salmon due to temperature fluctuations during superchilling
AbstractSuperchilling is an emerging technology for meat preservation; however, the temperature changes during the process have been commonly ignored. Thus, the effects of temperature fluctuations on meat quality during superchilling are yet to be evaluated. In our study, pork loins and salmon fillets were stored for several days (0, 8, 15, 23, and 30 d) under different temperature fluctuations based on −3.5 °C as the target temperature. The results showed that after 15 d of superchilling storage, the values of total volatile basic nitrogen, total viable count, and lipid oxidation were significantly (P<0.05) altered ...
Source: Journal of Zhejiang University. Science. B. - July 1, 2022 Category: Universities & Medical Training Source Type: research

Integrated metabolism and epigenetic modifications in the macrophages of mice in responses to cold stress
AbstractThe negative effects of low temperature can readily induce a variety of diseases. We sought to understand the reasons why cold stress induces disease by studying the mechanisms of fine-tuning in macrophages following cold exposure. We found that cold stress triggers increased macrophage activation accompanied by metabolic reprogramming of aerobic glycolysis. The discovery, by genome-wide RNA sequencing, of defective mitochondria in mice macrophages following cold exposure indicated that mitochondrial defects may contribute to this process. In addition, changes in metabolism drive the differentiation of macrophages ...
Source: Journal of Zhejiang University. Science. B. - June 1, 2022 Category: Universities & Medical Training Source Type: research

LncRNA-m18as1 competitively binds with miR-18a-5p to regulate follicle-stimulating hormone secretion through the Smad2/3 pathway in rat primary pituitary cells
In this study, a new lncRNA was identified for the first time. First, we analyzed the gene expression of lncRNA-m18as1 in different tissues and different stages by reverse transcript ion-quantitative polymerase chain reaction (RT-qPCR) and observed the localization of lncRNA-m18as1 with fluorescence in situ hybridization, which indicated that this lncRNA was distributed mainly in the cytoplasm. Next, we used RT-qPCR and enzyme-linked immunosorbent assay (ELISA) to analyze the re gulation of FSH synthesis and secretion after overexpression or knockdown of lncRNA-m18as1 and found that lncRNA-m18as1 was positively correlated ...
Source: Journal of Zhejiang University. Science. B. - June 1, 2022 Category: Universities & Medical Training Source Type: research

Roles of neutrophil reactive oxygen species (ROS) generation in organ function impairment in sepsis
AbstractSepsis is a condition of severe organ failure caused by the maladaptive response of the host to an infection. It is a severe complication affecting critically ill patients, which can progress to severe sepsis, septic shock, and ultimately death. As a vital part of the human innate immune system, neutrophils are essential in resisting pathogen invasion, infection, and immune surveillance. Neutrophil-produced reactive oxygen species (ROS) play a pivotal role in organ dysfunction related to sepsis. In recent years, ROS have received a lot of attention as a major cause of sepsis, which can progress to severe sepsis and...
Source: Journal of Zhejiang University. Science. B. - June 1, 2022 Category: Universities & Medical Training Source Type: research

Functional characterization of piggyBac-like elements from Nilaparvata lugens (St ål) (Hemiptera: Delphacidae)
AbstractPiggyBac is a transposable DNA element originally discovered in the cabbage looper moth (Trichoplusia ni). TheT. ni piggyBac transposon can introduce exogenous fragments into a genome, constructing a transgenic organism. Nevertheless, the comprehensive analysis of endogenouspiggyBac-like elements (PLEs) is important before usingpiggyBac, because they may influence the genetic stability of transgenic lines. Herein, we conducted a genome-wide analysis ofPLEs in the brown planthopper (BPH)Nilaparvata lugens (St ål) (Hemiptera: Delphacidae), and identified a total of 28PLE sequences. AllN. lugens piggyBac-like element...
Source: Journal of Zhejiang University. Science. B. - June 1, 2022 Category: Universities & Medical Training Source Type: research

Seasonal coronaviruses and SARS-CoV-2: effects of preexisting immunity during the COVID-19 pandemic
AbstractAlthough the coronavirus disease 2019 (COVID-19) epidemic is still ongoing, vaccination rates are rising slowly and related treatments and drugs are being developed. At the same time, there is increasing evidence of preexisting immunity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in humans, mainly consisting of preexisting antibodies and immune cells (including T cells and B cells). The presence of these antibodies is mainly due to the seasonal prevalence of four common coronavirus types, especially OC43 and HKU1. The accumulated relevant evidence has suggested that the target of antibodies...
Source: Journal of Zhejiang University. Science. B. - June 1, 2022 Category: Universities & Medical Training Source Type: research

Spirulina platensis aqueous extracts ameliorate colonic mucosal damage and modulate gut microbiota disorder in mice with ulcerative colitis by inhibiting inflammation and oxidative stress
AbstractUlcerative colitis (UC) is a chronic and recurrent inflammatory bowel disease (IBD) that has become a major gastroenterologic problem during recent decades. Numerous complicating factors are involved in UC development such as oxidative stress, inflammation, and microbiota disorder. These factors exacerbate damage to the intestinal mucosal barrier.Spirulina platensis is a commercial alga with various biological activity that is widely used as a functional ingredient in food and beverage products. However, there have been few studies on the treatment of UC usingS. platensis aqueous extracts (SP), and the underlying m...
Source: Journal of Zhejiang University. Science. B. - June 1, 2022 Category: Universities & Medical Training Source Type: research