Journal of Zhejiang University. Science. B. This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Application of a recombinant replicase to localize the Trionyx sinensis hemorrhagic syndrome virus and evaluate its effects on antiviral genes of T. sinensis
In this study, a partial protein of TSHSV-HP4 was produced using a prokaryotic expression system, and its polyclonal antibody was generated. The polyclonal antibody was confirmed by western blot and dot enzyme-linked immunosorbent assay (dot-ELISA). The distribution of TSHSV in different organs ofT. sinensis was examined by immunohistochemistry (IHC) and the expression of immune-related genes was analyzed using quantitative real-time polymerase chain reaction (qRT-PCR). The results indicated that the recombinant TSHSV-HP4 protein was successfully expressed, and the generated polyclonal antibody showed specific binding to v...
Source: Journal of Zhejiang University. Science. B. - April 1, 2021 Category: Universities & Medical Training Source Type: research

Development and validation of an individualized nomogram for early prediction of the duration of SARS-CoV-2 shedding in COVID-19 patients with non-severe disease
AbstractWith the number of cases of coronavirus disease-2019 (COVID-19) increasing rapidly, the World Health Organization (WHO) has recommended that patients with mild or moderate symptoms could be released from quarantine without nucleic acid retesting, and self-isolate in the community. This may pose a potential virus transmission risk. We aimed to develop a nomogram to predict the duration of viral shedding for individual COVID-19 patients. This retrospective multicentric study enrolled 135 patients as a training cohort and 102 patients as a validation cohort. Significant factors associated with the duration of viral sh...
Source: Journal of Zhejiang University. Science. B. - April 1, 2021 Category: Universities & Medical Training Source Type: research

Expression of Drosophila melanogaster acetylcholinesterase ( DmAChE ) gene splice variants in Pichia pastoris and evaluation of its sensitivity to organophosphorus pesticides
AbstractAcetylcholinesterase (AChE) is a key enzyme used to detect organophosphorus pesticide residues by the enzyme inhibition method. An accidental discovery of a mutant strain with AChE activity was made in our laboratory during the process of AChE expression byPichia pastoris. The pPIC9K-Drosophila melanogaster acetylcholinesterase (DmAChE)-like expression vector was constructed by codon optimization of this mutant strain, which was transformed intoP. pastoris GS115, and positive clones were selected on yeast peptone dextrose (YPD) plate with G418 at 4.0 mg/mL. The GS115-pPIC9K-DmAChE-like strain was subjected to 0.5% ...
Source: Journal of Zhejiang University. Science. B. - March 1, 2021 Category: Universities & Medical Training Source Type: research

A novel porous silica-zirconia coating for improving bond performance of dental zirconia
ConclusionsDip-coating with silica-zirconia might be a feasible way to improve resin-zirconia bonding. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - March 1, 2021 Category: Universities & Medical Training Source Type: research

Reduced glycodeoxycholic acid levels are associated with negative clinical outcomes of gestational diabetes mellitus
In conclusion, GDCA provides a valuable biomarker to evaluate HOMA-IR and DIo, and decreased GDCA levels predict poorer clinical outcomes for GDM. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - March 1, 2021 Category: Universities & Medical Training Source Type: research