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Circular RNAs: typical biomarkers for bone-related diseases
AbstractBone is a connective tissue that has important functions in the human body. Cells and the extracellular matrix (ECM) are key components of bone and are closely related to bone-related diseases. However, the outcomes of conventional treatments for bone-related diseases are not promising, and hence it is necessary to elucidate the exact regulatory mechanisms of bone-related diseases and identify novel biomarkers for diagnosis and therapy. Circular RNAs (circRNAs) are single-stranded RNAs that form closed circular structures without a 5 ′ cap or 3′ tail and polycyclic adenylate tails. Due to their high stability, ...
Source: Journal of Zhejiang University. Science. B. - December 1, 2022 Category: Universities & Medical Training Source Type: research

Globular adiponectin-mediated vascular remodeling by affecting the secretion of adventitial-derived tumor necrosis factor- α induced by urotensin II
ConclusionsFrom the results of our study, we can speculate that UII induces TNF- α protein and TNF-α-R secretion in AFs and rat tunica adventitia of vessels via the Rho and PKC signal transduction pathways. Thus, it is plausible that adiponectin is a major player in adventitial progression and could serve as a novel therapeutic target for cardiovascular disease administration. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - December 1, 2022 Category: Universities & Medical Training Source Type: research

Changes in the gut microbiota of osteoporosis patients based on 16S rRNA gene sequencing: a systematic review and meta-analysis
ConclusionsThis observational meta-analysis revealed that changes in the GM were correlated with OP, and variations in some advantageous GM might involve regional differences. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - December 1, 2022 Category: Universities & Medical Training Source Type: research

Application of regional citrate anticoagulation in patients at high risk of bleeding during intermittent hemodialysis: a prospective multicenter randomized controlled trial
ConclusionsCompared with saline flushing, the two-stage RCA combined with a sequential anticoagulation strategy significantly reduced extracorporeal circulation clotting events and prolonged the dialysis time without serious adverse events. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - November 1, 2022 Category: Universities & Medical Training Source Type: research

Recent advances in the use of the CRISPR-Cas system for the detection of infectious pathogens
AbstractInfectious diseases cause great economic loss and individual and even social anguish. Existing detection methods lack sensitivity and specificity, have a poor turnaround time, and are dependent on expensive equipment. In recent years, the clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein (Cas) system has been widely used in the detection of pathogens that cause infectious diseases owing to its high specificity, sensitivity, and speed, and good accessibility. In this review, we discuss the discovery and development of the CRISPR-Cas system, summarize related analysis and in...
Source: Journal of Zhejiang University. Science. B. - November 1, 2022 Category: Universities & Medical Training Source Type: research

A method for distinguishing benign and malignant pulmonary nodules based on 3D dual path network aided by K-means clustering analysis
概要目的为了提高肺癌早期诊断的准确性,本文使用机器学习,可以有效地帮助放射科医生区分肺结节的良恶性。创新点基于三维双路径网络（3DDPN）辅助K均值聚类分析区分良恶性肺结节,类别分析可以有效地表示良恶性肺结节的多种潜在亚型。方法在这项研究中,我们提出了一种基于3DDPN并辅以聚类分析来识别良恶性肺结节的新分类方案。首先,根据四位放射科医生的标注结果,从计算机断层扫描（CT）图像中截取以肺结节为中心,尺寸为64×64×64的像素单元;并训练pre-3D DPN模型提 取...
Source: Journal of Zhejiang University. Science. B. - November 1, 2022 Category: Universities & Medical Training Source Type: research

Clinical and immune response characteristics among vaccinated persons infected with SARS-CoV-2 delta variant: a retrospective study
ConclusionsTwo doses of the SARS-CoV-2-inactivated vaccine were highly effective at limiting symptomatic disease and reducing immune response, while a single dose did not seem to be effective. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - November 1, 2022 Category: Universities & Medical Training Source Type: research

High levels of serum IL-10 indicate disease progression, extramedullary involvement, and poor prognosis in multiple myeloma
概要多发性骨髓瘤（MM）细胞的生长和存活依赖于骨髓微环境,其中细胞因子常与疾病的不良预后有关。在新药治疗时代,血清细胞因子水平对MM预后作用需进一步评估。本研究分析了157例初诊MM患者外周血细胞因子水平（包括白细胞介素 -2（IL-2）、IL-4、IL-6、IL-10、干扰素γ（IFN-γ）、肿瘤坏死因子α（TNF-α）和IL-17A）与患者基线临床特征、疗效以及预后之间的关系。并选用15例年龄契合的健康志愿者和96例复发MM患者作为对照。所有患者均一线接受硼替佐米为基础 方案的治疗。...
Source: Journal of Zhejiang University. Science. B. - November 1, 2022 Category: Universities & Medical Training Source Type: research

Combination of Se-methylselenocysteine, D- α-tocopheryl succinate, β-carotene, and l-lysine can prevent cancer metastases using as an adjuvant therapy
ConclusionsThis study revealed the roles of MVBL in improving immunity and antioxidation, preventing tumor growth, and inhibiting metastasis in vitro and in vivo. MVBL may be used as an adjuvant drug in cancer therapy for improving the survival and quality of life of cancer patients. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - November 1, 2022 Category: Universities & Medical Training Source Type: research

Optimized thyroid transcription factor-1 core promoter-driven microRNA-7 expression effectively inhibits the growth of human non-small-cell lung cancer cells
In this study, we identified the core promoter of TTF-1 (from −1299 bp to −871 bp) by 5′ deletion assay and screened out the putative transcription factors nuclear factor-1 (NF-1) and activator protein-1 (AP-1). Further analysis revealed that the expression level of NF-1, but not AP-1, was positively connected with the activation of TTF-1 core promoter i n human non-small-cell lung cancer (NSCLC) cells. Moreover, the silencing of NF-1 could reduce the expression level of miR-7 operated by TTF-1 core promoter. Of note, we optimized four distinct sequences to form additional NF-1-binding sites (TGGCA) in the sequence o...
Source: Journal of Zhejiang University. Science. B. - November 1, 2022 Category: Universities & Medical Training Source Type: research

Tertiary lymphoid structures as unique constructions associated with the organization, education, and function of tumor-infiltrating immunocytes
AbstractTertiary lymphoid structures (TLSs) are formations at sites with persistent inflammatory stimulation, including tumors. These ectopic lymphoid organs mainly consist of chemo-attracting B cells, T cells, and supporting dendritic cells (DCs). Mature TLSs exhibit functional organization for the optimal development and collaboration of adaptive immune response, delivering an augmented effect on the tumor microenvironment (TME). The description of the positive correlation between TLSs and tumor prognosis is reliable only under a certain condition involving the localization and maturation of TLSs. Emerging evidence sugge...
Source: Journal of Zhejiang University. Science. B. - October 1, 2022 Category: Universities & Medical Training Source Type: research

Tumor immune checkpoints and their associated inhibitors
AbstractImmunological evasion is one of the defining characteristics of cancers, as the immune modification of an immune checkpoint (IC) confers immune evasion capabilities to tumor cells. Multiple ICs, such as programmed cell death protein-1 (PD-1) and cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4), can bind to their respective receptors and reduce tumor immunity in a variety of ways, including blocking immune cell activation signals. IC blockade (ICB) therapies targeting these checkpoint molecules have demonstrated significant clinical benefits. This is because antibody-based IC inhibitors and a variety of specific...
Source: Journal of Zhejiang University. Science. B. - October 1, 2022 Category: Universities & Medical Training Source Type: research

Relapse after CAR-T cell therapy in B-cell malignancies: challenges and future approaches
AbstractChimeric antigen receptor-T (CAR-T) cell therapy, as a novel cellular immunotherapy, has dramatically reshaped the landscape of cancer treatment, especially in hematological malignancies. However, relapse is still one of the most troublesome obstacles to achieving broad clinical application. The intrinsic factors and superior adaptability of tumor cells mark a fundamental aspect of relapse. The unique biological function of CAR-T cells governed by their special CAR construction also affects treatment efficacy. Moreover, complex cross-interactions among CAR-T cells, tumor cells, and the tumor microenvironment (TME) ...
Source: Journal of Zhejiang University. Science. B. - October 1, 2022 Category: Universities & Medical Training Source Type: research

UPF1 increases amino acid levels and promotes cell proliferation in lung adenocarcinoma via the eIF2 α-ATF4 axis
In this study, we found that UPF1 was significantly correlated with a portion of amino acid metabolic pathways in LUAD by integrating bioinformatics and metabolomics. We further confirmed that UPF1 knockdown inhibited activating transcription factor 4 (ATF4) and Ser51 phosphorylation of eukaryotic translation initiation factor 2 α (eIF2α), the core proteins in amino acid metabolism reprogramming. In addition, UPF1 promotes cell proliferation by increasing the amino-acid levels of LUAD cells, which depends on the function of ATF4. Clinically, UPF1 mRNA expression is abnormal in LUAD tissues, and higher expression of UPF1 ...
Source: Journal of Zhejiang University. Science. B. - October 1, 2022 Category: Universities & Medical Training Source Type: research

Secondary donor-derived CD19 CAR-T therapy is safe and efficacious in acute lymphoblastic leukemia with extramedullary relapse after first autologous CAR-T therapy
概要目的尽管嵌合抗原受T(CAR-T)细胞桥接异基因造血干细胞移植(allo-HSCT)对于难治/复发B细胞恶性肿瘤已经显示出了显著的治疗效果,但仍有一小部分患者会出现疾病复发。创新点供体来源的CAR-T细胞可通过移植物的抗白血病作用发挥抗肿瘤作用,且安全性较好,因此可作为移植后复发B系急性淋巴细胞白血病(B-ALL)治疗的选择之一。方法本研究报告了一位62岁的女性患者,她被诊断为CD19阳性B-ALL。在首次自体CAR-T桥接异基因造血干细胞治疗后,患者很快出现髓外复发。随后患者接受了...
Source: Journal of Zhejiang University. Science. B. - October 1, 2022 Category: Universities & Medical Training Source Type: research