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Iron metabolism, ferroptosis, and lncRNA in cancer: knowns and unknowns
AbstractCancer cells undergo substantial metabolic alterations to sustain increased energy supply and uncontrolled proliferation. As an essential trace element, iron is vital for many biological processes. Evidence has revealed that cancer cells deploy various mechanisms to elevate the cellular iron concentration to accelerate proliferation. Ferroptosis, a form of cell death caused by iron-catalyzed excessive peroxidation of polyunsaturated fatty acids (PUFAs), is a promising therapeutic target for therapy-resistant cancers. Previous studies have reported that long noncoding RNA (lncRNA) is a group of critical regulators i...
Source: Journal of Zhejiang University. Science. B. - October 1, 2022 Category: Universities & Medical Training Source Type: research

Cancer immunotherapy: an evolving paradigm
(Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - October 1, 2022 Category: Universities & Medical Training Source Type: research

Nanobubbles loaded with carbon quantum dots for ultrasonic fluorescence dual detection
概要为提高临床肿瘤检测效率和准确性,我们通过制备负载碳量子点(CQDs)的纳米泡进行超声荧光双重检测,探索多重成像。在本实验中,我们采用薄膜分散法制备了阳离子脂质体,并使用全氟戊烷作为纳米气泡的核心气体材料。通过电荷效 应将纳米气泡与带负电荷的碳量子点耦合,制备出利用超声对比和荧光检测进行双向诊断的检测剂,并对负载碳量子点脂质体纳米气泡的配方和制备进行了筛选。当全氟戊烷用量为5 µL,胆固醇与(2,3-二油酰基-丙基)-三甲基氯化铵(DOTA P)的质量比为1:1,...
Source: Journal of Zhejiang University. Science. B. - September 1, 2022 Category: Universities & Medical Training Source Type: research

CircRNA.0007127 triggers apoptosis through the miR-513a-5p/CASP8 axis in K-562 cells
Conclusions: CircRNA.0007127 regulates K-562 cell apoptosis through the miR-513a-5p/CASP8 axis, which can serve as a novel powerful molecular target for K-562 cells. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - September 1, 2022 Category: Universities & Medical Training Source Type: research

Development and application of bionic systems consisting of tumor-cell membranes
摘要恶性肿瘤严重威胁人类健康,近十年间,肿瘤治疗取得了突破性进展。肿瘤细胞膜仿生系统的发展进一步增强了肿瘤靶向策略。来源于自体的肿瘤细胞膜能够消除非生物因素,并显示出高度的生物相容性。此外,肿瘤的快速增殖和易于 培养使肿瘤细胞膜比其他类型的生物膜更容易获得。本文首先介绍并回顾细胞膜仿生纳米系统的提出及发展,并且重点描述了在药物递送、光热和成像、肿瘤疫苗方面的应用。其次,对其安全性及可能存在的问题进行讨论。最后提出 未来发展的可...
Source: Journal of Zhejiang University. Science. B. - September 1, 2022 Category: Universities & Medical Training Source Type: research

BCAT1 promotes lung adenocarcinoma progression through enhanced mitochondrial function and NF- κB pathway activation
Abstract目的探究BCAT1在肺腺癌发展过程中的生物学作用及其分子机制。创新点首次证实BCAT1通过调控线粒体功能和NF-κB 通路影响肺腺癌发展,为肺腺癌发展的分子机制研究和临床治疗提供了新见解。方法首先通过免疫组化确定BCAT1蛋白在临床肺腺癌患者癌与癌旁组织中的表达,然后采用慢病毒侵染法构建BCAT1过表达或敲低的A549稳转株并进行增殖、迁移和侵袭实验。此外分别通过裸鼠皮下成瘤实验研究BCAT1对肺腺癌体内成瘤与肿瘤生 长能力的影响;通过CCK8、qRT-PCR、活性氧与...
Source: Journal of Zhejiang University. Science. B. - September 1, 2022 Category: Universities & Medical Training Source Type: research

Use of folic acid nanosensors with excellent photostability for hybrid imaging
概要前哨淋巴结绘图和肿瘤边界的确定能有效减少手术干预及提高患者的无复发生存率,在癌症手术中发挥重要作用。自体荧光成像(AFI)技术可提高肿瘤检测率且优化手术干预范围,可惜目前尚无可用于混合成像的荧光标记药物,而且荧光 染料与叶酸结合时易出现光漂白现象。本研究首次报道了一种可用于同步混合成像的叶酸纳米传感器,其光稳定性优异且与AFI内窥镜兼容。经过量子点表面功能化处理后,该纳米传感器能够与MCF-7癌细胞有效结合,从而可使用普通AFI设 备进行同步混...
Source: Journal of Zhejiang University. Science. B. - September 1, 2022 Category: Universities & Medical Training Source Type: research

Reduced cell invasion may be a characteristic of placental defects in pregnant women of advanced maternal age at single-cell level
AbstractThe mechanisms underlying pregnancy complications caused by advanced maternal age (AMA) remain unclear. We analyzed the cellular signature and transcriptomes of human placentas in AMA women to elucidate these mechanisms. Placental tissues from two AMA women and two controls were used for single-cell RNA-sequencing (scRNA-seq). Controls consisted of AMA women who did not experience any pregnancy complications and pregnant women below the age of 35 years without pregnancy complications. Trophoblast cells were obtained from the placentas of another six pregnant women (three AMA women and three controls), and in-vitro ...
Source: Journal of Zhejiang University. Science. B. - September 1, 2022 Category: Universities & Medical Training Source Type: research

Biomarkers in oral immunotherapy
AbstractFood allergy (FA) is a global health problem that affects a large population, and thus effective treatment is highly desirable. Oral immunotherapy (OIT) has been showing reasonable efficacy and favorable safety in most FA subjects. Dependable biomarkers are needed for treatment assessment and outcome prediction during OIT. Several immunological indicators have been used as biomarkers in OIT, such as skin prick tests, basophil and mast cell reactivity, T cell and B cell responses, allergen-specific antibody levels, and cytokines. Other novel indicators also could be potential biomarkers. In this review, we discuss a...
Source: Journal of Zhejiang University. Science. B. - September 1, 2022 Category: Universities & Medical Training Source Type: research

Translationally controlled tumor protein: the mediator promoting cancer invasion and migration and its potential clinical prospects
AbstractTranslationally controlled tumor protein (TCTP) is a highly conserved multifunctional protein localized in the cytoplasm and nucleus of eukaryotic cells. It is secreted through exosomes and its degradation is associated with the ubiquitin-proteasome system (UPS), heat shock protein 27 (Hsp27), and chaperone-mediated autophagy (CMA). Its structure contains three α-helices and eleven β-strands, and features a helical hairpin as its hallmark. TCTP shows a remarkable similarity to the methionine-R-sulfoxide reductase B (MsrB) and mammalian suppressor of Sec4 (Mss4/Dss4) protein families, which exerts guanine nucleoti...
Source: Journal of Zhejiang University. Science. B. - August 1, 2022 Category: Universities & Medical Training Source Type: research

Research and application advances in rehabilitation assessment of stroke
AbstractStroke has a high incidence and disability rate, and rehabilitation is an effective means to reduce the disability rate of patients. To systematize rehabilitation assessment, which is the foundation for rehabilitation therapy, we summarize the assessment methods commonly used in research and clinical applications, including the various types of stroke rehabilitation scales and their applicability, and related biomedical detection technologies, including surface electromyography (sEMG), motion analysis systems, transcranial magnetic stimulation (TMS), magnetic resonance imaging (MRI), and combinations of different t...
Source: Journal of Zhejiang University. Science. B. - August 1, 2022 Category: Universities & Medical Training Source Type: research

Impact of COVID-19 pandemic on the pregnancy outcomes of women undergoing assisted reproductive techniques (ARTs): a systematic review and meta-analysis
AbstractThe global outbreak of the coronavirus disease 2019 (COVID-19) led to the suspension of most treatments with assisted reproductive technique (ART). However, with the recent successful control of the pandemic in China, there is an urgent public need to resume full reproductive care. To determine whether the COVID-19 pandemic had any adverse effects on female fertility and the pregnancy outcomes of women undergoing ART, a systematic review and meta-analysis was conducted using the electronic Chinese and English databases. Dichotomous outcomes were summarized as prevalence, and odds ratios (ORs) and continuous outcome...
Source: Journal of Zhejiang University. Science. B. - August 1, 2022 Category: Universities & Medical Training Source Type: research

Luminal androgen receptor (LAR) subtype of triple-negative breast cancer: molecular, morphological, and clinical features
AbstractAccording to the classification presented by Lehmann BD (2016), triple-negative breast cancer (TNBC) is a heterogeneous group of malignant tumors with four specific subtypes: basal-like (subtype 1 and subtype 2), mesenchymal, and luminal androgen receptor (LAR) subtypes. The basal-like subtypes of carcinomas predominate in this group, accounting for up to 80% of all cases. Despite the significantly lower proportions of mesenchymal and LAR variants in the group of breast carcinomas with a TNBC profile, such tumors are characterized by aggressive biological behavior. To this end, the LAR subtype is of particular inte...
Source: Journal of Zhejiang University. Science. B. - August 1, 2022 Category: Universities & Medical Training Source Type: research

Investigation and experimental validation of curcumin-related mechanisms against hepatocellular carcinoma based on network pharmacology
ConclusionsCurcumin can treat HCC through the p53 apoptotic pathway and the AMPK/Unc-51-like kinase 1 (ULK1) autophagy pathway, in which the mutual transformation of autophagy and apoptosis may occur through DRAM and p62. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - August 1, 2022 Category: Universities & Medical Training Source Type: research

Exosomes released by melanocytes modulate fibroblasts to promote keloid formation: a pilot study
概要瘢痕疙瘩是常见病理性皮肤愈合, 极难预防和根治。临床发现黑色素细胞活性较高的深肤色人群具有瘢痕疙瘩易感性, 提示黑色素细胞可能参与其形成过程。瘢痕疙瘩主要由成纤维细胞中TGF-β/Smads通路异常激活所致, 而文献报道黑 色素细胞分泌物能激活成纤维细胞中TGF-β信号, 但机制不明。我们前期研究发现携带特定miRNAs的黑色素细胞外泌体可激活成纤维细胞内TGF-β/Smads通路, 从而促进瘢痕疙瘩形成。本研究将从细胞间对话角度揭示深肤色人群易感瘢痕疙 瘩的内在机理。...
Source: Journal of Zhejiang University. Science. B. - August 1, 2022 Category: Universities & Medical Training Source Type: research