Journal of Zhejiang University. Science. B. This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Targeting ferroptosis and ferritinophagy: new targets for cardiovascular diseases
AbstractCardiovascular diseases (CVDs) are a leading factor driving mortality worldwide. Iron, an essential trace mineral, is important in numerous biological processes, and its role in CVDs has raised broad discussion for decades. Iron-mediated cell death, namely ferroptosis, has attracted much attention due to its critical role in cardiomyocyte damage and CVDs. Furthermore, ferritinophagy is the upstream mechanism that induces ferroptosis, and is closely related to CVDs. This review aims to delineate the processes and mechanisms of ferroptosis and ferritinophagy, and the regulatory pathways and molecular targets involved...
Source: Journal of Zhejiang University. Science. B. - January 1, 2024 Category: Universities & Medical Training Source Type: research

Role of 5-hydroxytryptamine type 3 receptors in the regulation of anxiety reactions
Abstract5-Hydroxytryptamine (5-HT) type 3 receptor (5-HT3R) is the only type of ligand-gated ion channel in the 5-HT receptor family. Through the high permeability of Na+, K+, and Ca2+ and activation of subsequent voltage-gated calcium channels (VGCCs), 5-HT3R induces a rapid increase of neuronal excitability or the release of neurotransmitters from axon terminals in the central nervous system (CNS). 5-HT3Rs are widely expressed in the medial prefrontal cortex (mPFC), amygdala (AMYG), hippocampus (HIP), periaqueductal gray (PAG), and other brain regions closely associated with anxiety reactions. They have a bidirectional r...
Source: Journal of Zhejiang University. Science. B. - January 1, 2024 Category: Universities & Medical Training Source Type: research

Deep learning-based radiomics allows for a more accurate assessment of sarcopenia as a prognostic factor in hepatocellular carcinoma
摘要肌少症是指因持续骨骼肌含量流失、强度和功能下降引起的综合征, 且与包括肝细胞癌(HCC)在内的肿瘤患者预后密切相关. 目前该病的检测手段局限且无统一标准. 本文旨在利用基于影像组学的深度学习(DL)技术评估肌少症, 用于肝癌患者行肝脏部分切除术或肝移植术的预后预测. 本研究回顾性纳入浙大一院肝癌手术切除492例(训练集+内部验证集)与肝癌肝移植173例患者(外部LT验证集), 东方肝胆医院肝癌切除患者161例(外部验证集), 并收集患者术前一个月内的腹部计算机断层�...
Source: Journal of Zhejiang University. Science. B. - January 1, 2024 Category: Universities & Medical Training Source Type: research

A case of vitiligo after COVID-19 vaccination: a possible role of thymic dysfunction
摘要最近,人们发现接种2019冠状病毒病(COVID-19)疫苗后出现了大量并发症,影响心脏、肾脏、胰腺、关节和皮肤。本文结合已报道的11例COVID-19疫苗(主要与信使RNA(mRNA)疫苗有关)接种后白癜风病例的数据和我们自己接种的1例非复制载体疫苗后的白癜风病例,分析探讨了其相关的病理机制。根据常见的原理,自身免疫性病变是通过病毒的抗原表位与某些人类蛋白质之间的分子模拟诱发的。我们认为这一过程的基础是胸腺负选择的破坏,导致自身反应性T细胞迁移到外周。在本文中,我们...
Source: Journal of Zhejiang University. Science. B. - December 1, 2023 Category: Universities & Medical Training Source Type: research

Fibrillarin promotes homologous recombination repair by facilitating the recruitment of recombinase RAD51 to DNA damage sites
摘要同源重组(HR)是一种高度精确的DNA双链断裂(DSB)损伤修复方式。HR修复的关键步骤是重组酶RAD51通过包裹单链DNA形成核酸蛋白纤维丝,进行同源模板搜索,并进行DNA链入侵反应,从而启动DNA修复合成。本研究发现,纤维蛋白(FBL)是重要的HR 调节因子。一旦发生DNA损伤,FBL就被招募到DSB位点,并直接与RAD51互作。细胞缺失FBL会导致RAD51在DNA损伤位点募集减少,HR修复效率降低。此外,细胞缺失FBL会导致染色体畸变增加,促使细胞对DNA损伤药物敏感。本研究提出了FBL介导的RAD5 1在DNA损伤位点的...
Source: Journal of Zhejiang University. Science. B. - December 1, 2023 Category: Universities & Medical Training Source Type: research

Luteolin suppresses oral carcinoma 3 (OC3) cell growth and migration via modulating polo-like kinase 1 (PLK1) expression and cellular energy metabolism
摘要尽管木犀草素的抗癌作用已在多种肿瘤模型中报道,但关于其在口腔癌中的作用研究鲜少。本研究探讨了木犀草素对口腔癌细胞OC3生长和迁移的影响及其机制。结果表明,木犀草素能够抑制或下调OC3细胞的增殖、细胞周期以及p53-Ser 15(P)和polo样激酶1(PLK1)蛋白水平。耗氧率(OCR)和胞外酸化率(ECAR)水平以及ATP产量也显著降低。Transwell试验进一步证实OC3细胞的迁移能力会受到木犀草素的影响。综上,本研究发现木犀草素能抑制口腔癌细胞OC3的增殖,其机制可能与p53- PLK1信号传导下调...
Source: Journal of Zhejiang University. Science. B. - December 1, 2023 Category: Universities & Medical Training Source Type: research

Roles of lncRNA in the diagnosis and prognosis of triple-negative breast cancer
AbstractBreast cancer is a malignant tumor that seriously endangers women ’s lives. The prognosis of breast cancer patients differs among molecular types. Compared with other subtypes, triple-negative breast cancer (TNBC) has been a research hotspot in recent years because of its high degree of malignancy, strong invasiveness, rapid progression, easy of recurrence, dist ant metastasis, poor prognosis, and high mortality. Many studies have found that long non-coding RNA (lncRNA) plays an important role in the occurrence, proliferation, migration, recurrence, chemotherapy resistance, and other characteristics of TNBC. Some...
Source: Journal of Zhejiang University. Science. B. - December 1, 2023 Category: Universities & Medical Training Source Type: research

Abscisic acid-mediated cytosolic Ca2+ modulates triterpenoid accumulation of Ganoderma lucidum
摘要灵芝是中医药宝库中的珍品,具有重要的药理学功能。作为灵芝最主要的活性物质之一,灵芝三萜含量是评价灵芝质量的重要指标。本研究旨在探讨脱落酸(ABA)介导胞内钙离子(Ca2+)对灵芝三萜积累的影响。研究发现,ABA(300 µmol/L)可有效促进灵芝三萜积累,其合成途径中关键酶基因(sqs、hmgr、ls) 表达水平随之发生变化;同时,ABA诱导可激活胞内Ca2+通道并提高Ca2+浓度。在ABA介导下,外源Ca2+供体和抑制剂可直接影响胞内Ca2+浓度及Ca2+信号相关基因的表达。此外,ABA介导的Ca2+在灵芝三萜...
Source: Journal of Zhejiang University. Science. B. - December 1, 2023 Category: Universities & Medical Training Source Type: research

Micropeptides: origins, identification, and potential role in metabolism-related diseases
AbstractWith the development of modern sequencing techniques and bioinformatics, genomes that were once thought to be noncoding have been found to encode abundant functional micropeptides (miPs), a kind of small polypeptides. Although miPs are difficult to analyze and identify, a number of studies have begun to focus on them. More and more miPs have been revealed as essential for energy metabolism homeostasis, immune regulation, and tumor growth and development. Many reports have shown that miPs are especially essential for regulating glucose and lipid metabolism and regulating mitochondrial function. MiPs are also involve...
Source: Journal of Zhejiang University. Science. B. - December 1, 2023 Category: Universities & Medical Training Source Type: research

Typical hemophagocytic syndrome associated with cytomegalovirus infection in an immunocompetent patient: a case report and literature review
摘要嗜血细胞综合征是一种罕见的因大量免疫细胞不受控制的激活和增殖以及细胞因子风暴引起的致命性疾病。尽管病毒感染是其常见病因,但由巨细胞病毒引起的嗜血细胞综合征较为少见。巨细胞病毒感染通常可在免疫功能低下或缺陷人群中引起系列疾病,而在免疫功能正常人群中多表现为无症状。本文报道了一例免疫功能正常患者感染巨细胞病毒引起嗜血细胞综合征后,经芦可替尼治疗成功好转。本文旨在强调免疫功能正常患者感染巨细胞病毒后可引起严重的嗜血细胞综�...
Source: Journal of Zhejiang University. Science. B. - December 1, 2023 Category: Universities & Medical Training Source Type: research

Proapoptotic protein Bim regulates the suppressive function of Treg cells
摘要调节性T细胞(Treg细胞)是一种特殊的免疫抑制性CD4+-T淋巴细胞亚群,具有维持机体免疫稳态的重要作用。有研究证实,促凋亡蛋白Bim参与Treg细胞功能的调控,但是还没有使用条件性敲除小鼠对Bim在Treg细胞中功能研究的报道,且Treg细胞中 Bim发挥功能的机制也不清楚。已知Bim在衰老的Treg细胞中丰度下调,然而该过程在Treg细胞衰老过程中的具体作用尚不清楚。本研究中,作者繁育产生Foxp3Cre;Bimfl/fl条件性敲除小鼠,特异性在Treg细胞中敲除Bim。Foxp3Cre;Bimfl/fl小鼠可育,但是出现多器官炎�...
Source: Journal of Zhejiang University. Science. B. - December 1, 2023 Category: Universities & Medical Training Source Type: research

HPCAL1 is a novel driver of autophagy-dependent ferroptosis
摘要自噬是细胞内一种高度保守的生理过程,可通过溶酶体系统降解过量或受损的细胞器、有毒的蛋白聚集体和病原体等。最新研究表明,海马钙素样1(HPCAL1)可作为特异性自噬受体和铁死亡的正调节因子。HPCAL1可选择性降解钙粘素2(CDH 2),加速脂质过氧化,促进癌细胞铁死亡。iHPCAL1是抑制HPCAL1的小分子化合物,可抑制 Erastin诱导的肿瘤细胞铁死亡。此外,它还可以抑制铁死亡诱导的急性胰腺炎。本文通过对HPCAL1在铁死亡中的具体作用机制进行概述,为HPCAL1作为铁死亡相关疾病的潜�...
Source: Journal of Zhejiang University. Science. B. - November 1, 2023 Category: Universities & Medical Training Source Type: research

Advantages of contrast-enhanced ultrasound in the localization and diagnostics of sentinel lymph nodes in breast cancer
AbstractSentinel lymph nodes (SLNs) are the first station of lymph nodes that extend from the breast tumor to the axillary lymphatic drainage. The pathological status of these LNs can predict that of the entire axillary lymph node. Therefore, the accurate identification of SLNs is necessary for sentinel lymph node biopsy (SLNB) to replace axillary lymph node dissection (ALND). The quality of life and prognosis of breast cancer patients are related to proper surgical treatment after the precise identification of SLNs. Some of the SLN tracers that have been identified include radioisotope, nano-carbon, indocyanine green (ICG...
Source: Journal of Zhejiang University. Science. B. - November 1, 2023 Category: Universities & Medical Training Source Type: research

Fucoidan sulfate from Sargassum fusiforme regulates the SARS-CoV-2 receptor AXL expression in human embryonic lung diploid fibroblast cells
摘要新冠病毒感染疫情严重威胁着世界各国人民的生命健康。目前,对病毒感染的防治研究主要集中在抑制病毒与分子受体的结合上。AXL作为新发现的严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)受体,在协助病毒感染人体呼吸系统中发挥着重要作用,是未来临床干预的潜在靶点。本研究对已发表的单细胞测序数据进行整理和分析,发现AXL在年轻人肺细胞中的表达水平明显高于老年人。人胚肺二倍体成纤维细胞(2BS)是衰老研究的公认细胞株。本文采用2BS细胞构建复制性细胞衰老模型,...
Source: Journal of Zhejiang University. Science. B. - November 1, 2023 Category: Universities & Medical Training Source Type: research

Loss-of-function of zebrafish cdt1 causes retarded body growth and underdeveloped gonads resembling human Meier-Gorlin syndrome
摘要染色质许可和DNA复制因子1(Cdt1)是复制起始许可的主要调控因子,也是组成复制前复合物的核心成员。细胞通过依赖Cdt1的波动水平,且在每个周期中通过调节其总量以确保DNA仅复制一次。Cdt1功能缺陷会造成DNA过度复制,最终导致基因组不稳定。虽然酵母中cdt1和人类Meier-Gorlin综合征(MGS)患者中的CDT1已被广泛研究,但缺乏脊椎动物模型。我们发现在硬骨鱼类分支的几个鲤形目物种(包括斑马鱼)中,Cdt1蛋白在其N末端插入一段其他脊椎动物中没有的独特无序序列。通过分析在cdt1基因...
Source: Journal of Zhejiang University. Science. B. - November 1, 2023 Category: Universities & Medical Training Source Type: research