Journal of Zhejiang University. Science. B. 
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概要炎症性肠病(IBD)是一组慢性、复发性肠道炎症, 主要包括溃疡性结肠炎(UC)和克罗恩病(CD)。IBD的发病机制很复杂, 其潜在机制仍不清楚。近年来, 人们对长链非编码RNA(lncRNA)进行了研究, 并将其确定为IBD的潜在生物标志物。在本研究中 , 通过使用高通量RNA测序筛选出一种新的lncRNA—lnc78583。前期荧光定量聚合酶链式反应(qRT-PCR)分析结果表明, lnc78583和同源框B13(HOXB13)的mRNA表达水平在IBD患者和炎症状态下的人结直肠黏膜上皮细胞(FHC)中显著降低。为了探索lnc78583在 肠道炎症环境...
Source: Journal of Zhejiang University. Science. B. - May 1, 2022 Category: Universities & Medical Training Source Type: research
Physicochemical properties, molecular structure, antioxidant activity, and biological function of extracellular melanin from Ascosphaera apis
This study is the first report on melanin produced byA. apis, providing an important background reference for further study on its role inA. apis. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - May 1, 2022 Category: Universities & Medical Training Source Type: research
Therapeutic potential of traditional Chinese medicine for vascular endothelial growth factor
AbstractVascular endothelial growth factor (VEGF) is the main regulator of physiological angiogenesis during embryonic development, bone growth, and reproductive function, and it also participates in a series of pathological changes. Traditional Chinese medicine (TCM), with a history of more than 2000 years, has been widely used in clinical practice, while the exploration of its mechanisms has only begun. This review summarizes the research of recent years on the influence of TCM on VEGF. It is found that many Chinese medicines and recipes have a regulatory effect on VEGF, indicating that Chinese medicine has broad prospec...
Source: Journal of Zhejiang University. Science. B. - May 1, 2022 Category: Universities & Medical Training Source Type: research
Prediction of birth weight in pregnancy with gestational diabetes mellitus using an artificial neural network
概要目的建立一个预测妊娠期糖尿病新生儿出生体重的人工神经网络模型, 并评估其预测的准确性。创新点妊娠期糖尿病新生儿出生体重的预测十分重要, 但目前预测精度欠佳。本研究利用大样本量的临床数据, 突破传统统计学方法, 应用机器学习建立了一个基于人工神经网络的预测模型, 其预测精度较传统方法有明显提升。方法收集2462名妊娠期糖尿病孕妇的临床数据, 其中80%的数据用于构建一个前馈神经网络模型, 并用反向传播算法和10折交叉验证法训练和优化;剩余20%的数据...
Source: Journal of Zhejiang University. Science. B. - May 1, 2022 Category: Universities & Medical Training Source Type: research
Proteomic characterization of four subtypes of M2 macrophages derived from human THP-1 cells
In this study, we systematically analyzed the protein expression profiles of human THP-1-derived macrophages (M0, M1, M2a, M2b, M2c, and M2d) using quantitative proteomics approaches. The commonly and specially regulated proteins of the four M2 subtypes and their potential biological functions were further investigated. The results showed that M2a and M2b, and M2c and M2d have very similar protein expression profiles. These data could serve as an important resource for studies of macrophages using THP-1 cells, and provide a reference to distinguish different M2 subtypes in macrophage-associated diseases for subsequent clin...
Source: Journal of Zhejiang University. Science. B. - May 1, 2022 Category: Universities & Medical Training Source Type: research
Can SpRY recognize any PAM in human cells?
AbstractThe application of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) can be limited due to a lack of compatible protospacer adjacent motif (PAM) sequences in the DNA regions of interest. Recently, SpRY, a variant ofStreptococcus pyogenes Cas9 (SpCas9), was reported, which nearly completely fulfils the PAM requirement. Meanwhile, PAMs for SpRY have not been well addressed. In our previous study, we developed the PAM Definition by Observable Sequence Excision (PAM-DOSE) and green fluorescent protein (GFP)-reporter systems to study PAMs in human cells. Herein, we e...
Source: Journal of Zhejiang University. Science. B. - May 1, 2022 Category: Universities & Medical Training Source Type: research
Romidepsin (FK228) improves the survival of allogeneic skin grafts through downregulating the production of donor-specific antibody via suppressing the IRE1 α-XBP1 pathway
In conclusion, FK228 could decrease the production of antibodies by B cells via inhibition of the IRE1α-XBP1 signaling pathway. Thus, FK228 is considered as a promising therapeutic agent for the clinical treatment of AMR. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - May 1, 2022 Category: Universities & Medical Training Source Type: research
Retraction Note to: Radiofrequency ablation versus hepatic resection for breast cancer liver metastasis: a systematic review and meta-analysis
The authors have retracted this article (Xiao et al., 2018). After publication they became aware that a number of the studies included in their analysis did not meet the eligibility criteria and that errors were made in classification and statistical analysis. The conclusions presented are therefore unreliable. All authors agree with this retraction. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - April 30, 2022 Category: Universities & Medical Training Source Type: research
A novel anticancer property of Lycium barbarum polysaccharide in triggering ferroptosis of breast cancer cells
In this study, human breast cancer cells (Michigan Cancer Foundation-7 (MCF-7) and MD Anderson-Metastatic Breast-231 (MDA-MB-231)) were treated with LBP. LBP inhibited their viability and proliferation in association with high levels of ferroptosis. Therefore, we aimed to ascertain whether LBP reduced cell viability through ferroptosis. We found that the structure and function of mitochondria, lipid peroxidation, and expression of solute carrier family 7 member 11 (SLC7A11, also known as xCT, the light-chain subunit of cystine/glutamate antiporter system Xc−) and glutathione peroxidase 4 (GPX4) were altered by LBP. Moreo...
Source: Journal of Zhejiang University. Science. B. - April 1, 2022 Category: Universities & Medical Training Source Type: research
Comparative transcriptome analysis of candidate genes involved in chlorogenic acid biosynthesis during fruit development in three pear varieties of Xinjiang Uygur Autonomous Region
摘要目的通过转录组学分析挖掘与新疆梨果中绿原酸生物合成相关的功能基因, 为未来新疆梨的功能性育种提供科学依据。创新点明确了在三种新疆梨果发育过程中, 与绿原酸含量变化趋势和绿原酸生物合成相关的差异基因及关键限制性酶。方法在鸭梨、库尔勒香梨和圆黄等三种梨果的不同发育阶段采集果实样品, 经液相色谱法分析不同发育期果品的绿原酸含量和熊果苷含量差异, 然后选择花后88、103和163天这三个绿原酸含量变化典型的时间点样品提取RNA, 进行转录组测序 , 采...
Source: Journal of Zhejiang University. Science. B. - April 1, 2022 Category: Universities & Medical Training Source Type: research
Efficient genetic transformation and CRISPR/Cas9-mediated genome editing of watermelon assisted by genes encoding developmental regulators
摘要目的克服西瓜遗传转化瓶颈, 建立西瓜高效遗传转化体系, 并以此为基础构建西瓜基因编辑体系, 促进西瓜功能基因组学和基因编辑育种研究。创新点通过比较多个生长调节基因和农杆菌菌株, 选取生长调节基因AtGRF5和农杆菌菌株GV3101, 在西瓜中建立了高效且不依赖于基因型的转化体系, 实现了西瓜突变体的快速创制。方法通过测试生长调节因子基因对西瓜转化率的影响, 发现多个生长调节基因可以提高西瓜的遗传转化效率。其中AtGRF5可将西瓜的转化效率0.88%左右提高至24.73...
Source: Journal of Zhejiang University. Science. B. - April 1, 2022 Category: Universities & Medical Training Source Type: research
Toxoplasma gondii infection induces cell apoptosis via multiple pathways revealed by transcriptome analysis
In conclusion, theT. gondii RH strain could promote and mediate apoptosis through multiple pathways mentioned above in Vero cells. Our findings improve the understanding of theT. gondii infection process through providing new insights into the related cellular apoptosis mechanisms. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - April 1, 2022 Category: Universities & Medical Training Source Type: research
Molecular pathogenesis of acetaminophen-induced liver injury and its treatment options
AbstractAcetaminophen, also known asN-acetyl-p-aminophenol (APAP), is commonly used as an antipyretic and analgesic agent. APAP overdose can induce hepatic toxicity, known as acetaminophen-induced liver injury (AILI). However, therapeutic doses of APAP can also induce AILI in patients with excessive alcohol intake or who are fasting. Hence, there is a need to understand the potential pathological mechanisms underlying AILI. In this review, we summarize three main mechanisms involved in the pathogenesis of AILI: hepatocyte necrosis, sterile inflammation, and hepatocyte regeneration. The relevant factors are elucidated and d...
Source: Journal of Zhejiang University. Science. B. - April 1, 2022 Category: Universities & Medical Training Source Type: research
Shoot rot of Zizania latifolia and the first record of its pathogen Pantoea ananatis in China
AbstractThe aquatic grassZizania latifolia grows symbiotically with the fungusUstilago esculenta producing swollen structures called Jiaobai, widely cultivated in China. A new disease ofZ. latifolia was found in Zhejiang Province, China. Initial lesions appeared on the leaf sheaths or sometimes on the leaves near the leaf sheaths. The lesions extended along the axis of the leaf shoots and formed long brown to dark brown streaks from the leaf sheath to the leaf, causing sheath rot and death of entire leaves on young plants. The pathogen was isolated and identified as the bacteriumPantoea ananatis, based on 16S ribosomal RNA...
Source: Journal of Zhejiang University. Science. B. - April 1, 2022 Category: Universities & Medical Training Source Type: research
Comparative metabolomics provides novel insights into the basis of petiole color differences in celery (Apium graveolens L.)
This study explored the differences in the types and contents of metabolites in celery cultivars with different-colored petioles and identified key substances for color formation. The results provide a theoretical basis and technical support for genetic improvement of celery petiole color. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - April 1, 2022 Category: Universities & Medical Training Source Type: research
