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Aqueous extracts from Tenebrio molitor larval and pupal stages inhibit early hepatocarcinogenesis in vivo
概要肝细胞癌 (HCC) 具有复杂的肿瘤内异质性, 这会导致治疗失败. 传统医学和实验研究表明, 食用昆虫具有治疗包括 HCC 在内的不同疾病的潜力. 我们在获得黄粉虫水提物后, 通过组织病理学、 免疫组织化学、 蛋白质印迹和影像学分析 确定其有效成分, 并比较黄粉虫三个生命阶段 (包括幼虫、 蛹和成虫) 的水提物对二乙基亚硝胺 (DEN) 诱导的小鼠早期 HCC 的影响. 结果表明, 与黄粉虫蛹的水提取物相比, 幼虫水提物能更显着地通过抗增殖机制来抑制早期肝癌的发生 . (Source: Journal of...
Source: Journal of Zhejiang University. Science. B. - December 1, 2021 Category: Universities & Medical Training Source Type: research

Response of Escherichia coli to hydrogen nanobubbles: an in vitro evaluation using synchrotron infrared spectroscopy
摘要目的探究分子氢在细胞水平上的作用机制.创新点使用同步辐射红外光谱技术评估了氢纳米气泡在细胞水平上对大肠杆菌的影响, 扩展了同步辐射红外光谱的应用范围.方法将大肠杆菌进行氢气纳米气泡处理后, 使用同步辐射红外光谱测定其红外光谱, 采用主成分分析等方法分析细胞成分的变化.结论氢纳米气泡处理后细胞的蛋白和脂肪酸成分发生较大改变, 而核酸和多糖成分则没有明显变化。这些结果表明, 氢气的作用靶点可能是细胞膜。此外, 氢气分子的作用与传统的抗氧...
Source: Journal of Zhejiang University. Science. B. - November 1, 2021 Category: Universities & Medical Training Source Type: research

Effect of Palrnatine on lipopolysaccharide-induced acute lung injury by inhibiting activation of the Akt/NF- κB pathway
AbstractInflammation plays an important role in the development of acute lung injury (ALI). Severe pulmonary inflammation can cause acute respiratory distress syndrome (ARDS) or even death. Expression of proinflammatory interleukin-1 β (IL-1β) and inducible nitric oxide synthase (iNOS) in the process of pulmonary inflammation will further exacerbate the severity of ALI. The purpose of this study was to explore the effect of Palrnatine (Pa) on lipopolysaccharide (LPS)-induced mouse ALI and its underlying mechanism. Pa, a natura l product, has a wide range of pharmacological activities with the potential to protect against...
Source: Journal of Zhejiang University. Science. B. - November 1, 2021 Category: Universities & Medical Training Source Type: research

Tumor-associated macrophages in osteosarcoma
AbstractOsteosarcoma (OS) is the most common primary bone tumor in children and adolescents. It is an aggressive tumor with a tendency to spread to the lung, which is the most common site of metastasis. Patients with advanced OS with metastases have poor prognoses despite the application of chemotherapy, thus highlighting the need for novel therapeutic targets. The tumor microenvironment (TME) of OS is confirmed to be essential for and supportive of tumor growth and dissemination. The immune component of the OS microenvironment is mainly composed of tumor-associated macrophages (TAMs). In OS, TAMs promote tumor growth and ...
Source: Journal of Zhejiang University. Science. B. - November 1, 2021 Category: Universities & Medical Training Source Type: research

NEDD8-conjugating enzyme E2 UBE2F confers radiation resistance by protecting lung cancer cells from apoptosis
摘要目的放射治疗是非小细胞肺癌的重要治疗手段之一, 而放疗抵抗限制了放疗的疗效, 导致患者生存率低。因此, 鉴定新型的放射增敏靶标将具有重要的临床实践意义.创新点揭示灭活NEDD8结合酶(E2)UBE2F可以诱导凋亡蛋白NOXA介导的细胞凋亡, 提高肺癌细胞的放疗敏感性。鉴于UBE2F在肺癌放射增敏治疗中的潜在作用, 靶向UBE2F有望成为一种新型有效的肺癌放射增敏策略.方法利用免疫印迹检测UBE2F和NOXA的表达情况; 用台盼蓝染色法检测放射和双氧水处理后肺癌细胞的存活率; 通过裸...
Source: Journal of Zhejiang University. Science. B. - November 1, 2021 Category: Universities & Medical Training Source Type: research

Urinary donor-derived cell-free DNA as a non-invasive biomarker for BK polyomavirus-associated nephropathy
This study investigated whether donor-derived cell-free DNA (ddcfDNA) can be used to differentiate BKPyVAN. Target region capture sequencing was applied to detect the ddcfDNAs of 12 recipients with stable graft function 22 with type I TCMR, 21 with proven BKPyVAN and 5 with possible PyVAN. We found that urinary ddcfDNA levels were upregulated in recipients with graft injury whereas plasma ddcfDNA levels were comparable for all groups. The median urinary concentrations and fractions of ddcfDNA in proven BKPyVAN recipients were significantly higher than those in type I TCMR recipients (10.4 vs. 6.1 ng/mL,P<0.001 and 68.4%...
Source: Journal of Zhejiang University. Science. B. - November 1, 2021 Category: Universities & Medical Training Source Type: research

Red light enhances folate accumulation in wheat seedlings
AbstractRed, white, blue, green, and yellow lights were applied to investigate their effects on folate accumulation in wheat seedlings. The different lights, especially red light, significantly increased the total folate content. Total folate showed maximum accumulation under 30 µmol/(m2·s) of red light, with an increase of 24% compared with the control (darkness). 5-Methyl-tetrahydrofolate (5-CH3-THF) was the dominant folate component, and was significantly increased by red light irradiation. In addition, under red light, the folate content of leaves was higher and more sensitive to light than that of endosperm or roots...
Source: Journal of Zhejiang University. Science. B. - November 1, 2021 Category: Universities & Medical Training Source Type: research

Cathepsin D knockdown regulates biological behaviors of granulosa cells and affects litter size traits in goats
This study used immunohistochemistry to analyze CTSD localization in goat ovarian tissues. Moreover, western blotting was applied to examine the differential expression of CTSD in the ovarian tissues of monotocous and polytocous goats. Subsequently, the effects of CTSD knockdown on cell proliferation, apoptosis, cell cycle, and the expression of candidate genes of the prolific traits, including bone morphogenetic protein receptor IB (BMPR-IB), follicle-stimulating hormone (FSHR), and inhibin α (INHA), were determined in granulosa cells. Results showed that CTSD was expressed in corpus luteum, follicle, and granulosa cells...
Source: Journal of Zhejiang University. Science. B. - November 1, 2021 Category: Universities & Medical Training Source Type: research

Tartary buckwheat database (TBD): an integrative platform for gene analysis of and biological information on Tartary buckwheat
摘要本研究首次构建了苦荞数据库, 该数据库提供了苦荞基因在不同组织和不同阶段果实中表达量, 苦荞基因的荧光定量引物, 以及苦荞转录因子家族生物信息学信息。我们在苦荞不同组织和不同发育阶段果实转录组数据中收集苦荞基 因的表达图谱, 共检测到31 839个基因, 其中在根中表达的有26 426个基因, 在茎中表达的有24 716基因, 在叶中表达的有24 227个基因, 在花中表达的有27 533个基因。并利用Primer 3软件为每个苦荞基因都提供了5对荧光定量引物。此外, 我们 还从苦荞基因组...
Source: Journal of Zhejiang University. Science. B. - November 1, 2021 Category: Universities & Medical Training Source Type: research

Heme oxygenase-1/carbon monoxide signaling participates in the accumulation of triterpenoids of Ganoderma lucidum
This study focused on GT accumulation in mycelia ofG. lucidum mediated by the heme oxygenase-1 (HO-1)/carbon monoxide (CO) signaling. Compared with the control, hemin (10 µmol/L) induced an increase of 60.1% in GT content and 57.1% in HO-1 activity. Moreover, carbon monoxide-releasing molecule-2 (CORM-2), CO donor, increased GT content by 56.0% and HO-1 activity by 18.1%. Zn protoporphyrin IX (ZnPPIX), a specific HO-1 inhibitor, significantly reduced GT content by 2 6.0% and HO-1 activity by 15.8%, while hemin supplementation reversed these effects. Transcriptome sequencing showed that HO-1/CO could function directly as a...
Source: Journal of Zhejiang University. Science. B. - November 1, 2021 Category: Universities & Medical Training Source Type: research

Impact of antitumor regimens on the outcomes of cancer patients with COVID-19: a pooled analysis
概要不同的肿瘤治疗方案可能是肿瘤合并新型冠状病毒肺炎(COVID-19)患者预后较差的原因之一, 但是目前相关研究却仍未有定论. 因此, 全面深入的分析可以辅助临床医生选择和制定新型冠状病毒流行期间的抗肿瘤治疗方案. 我们通过从 PubMed、 Embase和Cochrane数据库检索得到的提供了每个患者临床信息的11项相关研究展开汇总分析. 本研究发现化疗是这些COVID-19合并肿瘤的患者中最常见的抗肿瘤治疗. 经过对混杂因素的调整, 抗肿瘤治疗方案对患者结局的影响并不显 著. 值得关注的...
Source: Journal of Zhejiang University. Science. B. - October 1, 2021 Category: Universities & Medical Training Source Type: research

A proteomic analysis of Bcl-2 regulation of cell cycle arrest: insight into the mechanisms
AbstractB cell lymphoma 2 (Bcl-2) is an important antiapoptotic gene that plays a dual role in the maintenance of the dynamic balance between the survival and death of cancer cells. In our previous study, Bcl-2 was shown to delay the G0/G1 to S phase entry by regulating the mitochondrial metabolic pathways to produce lower levels of adenosine triphosphate (ATP) and reactive oxygen species (ROS). However, the detailed molecular mechanisms or pathways by which Bcl-2 regulates the cell cycle remain unknown. Here, we compared the effects of Bcl-2 overexpression with an empty vector control in the NIH3T3 cell line synchronized ...
Source: Journal of Zhejiang University. Science. B. - October 1, 2021 Category: Universities & Medical Training Source Type: research

Current progress of computational modeling for guiding clinical atrial fibrillation ablation
AbstractAtrial fibrillation (AF) is one of the most common arrhythmias, associated with high morbidity, mortality, and healthcare costs, and it places a significant burden on both individuals and society. Anti-arrhythmic drugs are the most commonly used strategy for treating AF. However, drug therapy faces challenges because of its limited efficacy and potential side effects. Catheter ablation is widely used as an alternative treatment for AF. Nevertheless, because the mechanism of AF is not fully understood, the recurrence rate after ablation remains high. In addition, the outcomes of ablation can vary significantly betwe...
Source: Journal of Zhejiang University. Science. B. - October 1, 2021 Category: Universities & Medical Training Source Type: research

Transcriptional memory and response to adverse temperatures in plants
AbstractTemperature is one of the major environmental signals controlling plant development, geographical distribution, and seasonal behavior. Plants perceive adverse temperatures, such as high, low, and freezing temperatures, as stressful signals that can cause physiological defects and even death. As sessile organisms, plants have evolved sophisticated mechanisms to adapt to recurring stressful environments through changing gene expression or transcriptional reprogramming. Transcriptional memory refers to the ability of primed plants to remember previously experienced stress and acquire enhanced tolerance to similar or d...
Source: Journal of Zhejiang University. Science. B. - October 1, 2021 Category: Universities & Medical Training Source Type: research

3D-printed models improve surgical planning for correction of severe postburn ankle contracture with an external fixator
In this study, patients coming from two centers were divided into two cohorts (3DPM group vs. control group) depending on whether a 3DPM was used for preoperative surgical planning. Operation duration, improvement in metatarsal-tibial angle (MTA), range of motion (ROM), the American Orthopedic Foot and Ankle Society (AOFAS) scores, complications, and patient-reported satisfaction were compared between two groups. The 3DPM group had significantly shorter operation duration than the control group ((2.0 ±0.3) h vs. (3.2± 0.3) h,P<0.01). MTA, ROM, and AOFAS scores between the two groups showed no significant differences p...
Source: Journal of Zhejiang University. Science. B. - October 1, 2021 Category: Universities & Medical Training Source Type: research