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Modulating effects of Astragalus polysaccharide on immune disorders via gut microbiota and the TLR4/NF- κB pathway in rats with syndrome of dampness stagnancy due to spleen deficiency
This study provides an insight into the function of APS as a unique potential prebiotic through exerting systemic activities in treating DSSD. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - July 1, 2023 Category: Universities & Medical Training Source Type: research

Suppression of NLRP3 inflammasome by ivermectin ameliorates bleomycin-induced pulmonary fibrosis
This study aimed to explore the ability of ivermectin (0.6 mg/kg) to alleviate bleomycin-induced biochemical derangements and histological changes in an experimental PF rat model. This can provide the means to validate the clinical utility of ivermectin as a treatment option for idiopathic PF. The results showed that ivermectin mitigated the bleomycin-evoked pulmonary injury, as manifested by the reduced infiltration of inflammatory cells, as well as decreased the inflammation and fibrosis scores. Intriguingly, ivermectin decreased collagen fiber deposition and suppressed transforming growth factor- β1 (TGF-β1) and fibro...
Source: Journal of Zhejiang University. Science. B. - July 1, 2023 Category: Universities & Medical Training Source Type: research

Construction and evaluation of in-house methylation-sensitive SNaPshot system and three classification prediction models for identifying the tissue origin of body fluid
In conclusion, the in-house SNaPshot system and RF prediction model could achieve accurate tissue origin identification of body fluids. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - June 27, 2023 Category: Universities & Medical Training Source Type: research

Transportation of citrinin is regulated by the CtnC gene in the medicinal fungus Monascus purpureus
摘要药食两用真菌红曲霉转运真菌毒素桔霉素的途径目前尚不清楚. 本文通过CRISPR/Cas9基因编辑和过表达, 分析了紫色红曲霉中一个假定的主要协同转运蛋白超家族(MFS)基因CtnC, 证实了CtnC参与桔霉素的外排以及抑制菌丝体中桔霉素的积累, 但CtnC蛋白不是唯一通道. 同时, 本研究结果发现CtnC过表达会负调控桔霉素合成相关基因(CtnD、CtnE、CtnF和pksCT) 的表达. 综上, 本研究发现的红曲霉中桔霉素转运体基因为真菌毒素的防控策略研究提供了新的依据. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - June 1, 2023 Category: Universities & Medical Training Source Type: research

Nanosilver alleviates foreign body reaction and facilitates wound repair by regulating macrophage polarization
In conclusion, dermal scaffolds containing NAg suppressed the foreign body reaction by regulating macrophages and the expression of inflammatory cytokines, thereby promoting wound healing. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - June 1, 2023 Category: Universities & Medical Training Source Type: research

Inflammatory granuloma of the trachea: a rare case with Epstin-Barr virus infection
摘要EB 病毒是人类疱疹病毒4型(EBV), 其感染可导致不常见的并发症. 本文报道的患者气道中出现了与EBV感染相关的炎症性肉芽肿, 入院后紧急接受了气管内肿物切除术. 他的气道组织经宏基因组下一代测序(mNGS)测试后, 被确认为EB病毒感染 . 在术后第30天, 患者接受支气管镜复查时发现声门下方气管壁上出现多个结节性粘膜隆起, 且其病理学检查结果发现炎性肉芽组织. 因此, 推测患者气道内出现的肉芽肿组织可能是由于EB病毒慢性感染导致的. 虽然类似的肉芽肿在其 他组织中的产...
Source: Journal of Zhejiang University. Science. B. - June 1, 2023 Category: Universities & Medical Training Source Type: research

Engineered Bacillus subtilis alleviates intestinal oxidative injury through Nrf2-Keap1 pathway in enterotoxigenic Escherichia coli (ETEC) K88-infected piglet
In this study, we built the recombinantBacillus subtilis WB800 expressing antimicrobial peptide KR32 (WB800-KR32) using genetic engineering methods and investigated its protective effects of nuclear factor-E2-related factor 2 (Nrf2)-Kelch-like ECH-associated protein 1 (Keap1) pathway activation in intestinal oxidative disturbance induced by enterotoxigenicEscherichia coli (ETEC) K88 in weaned piglets. Twenty-eight weaned piglets were randomly distributed into four treatment groups with seven replicates fed with a basal diet. The feed of the control group (CON) was infused with normal sterilized saline; meanwhile, the ETEC,...
Source: Journal of Zhejiang University. Science. B. - June 1, 2023 Category: Universities & Medical Training Source Type: research

Fibroblast growth factor 21 (FGF21) attenuates tacrolimus-induced hepatic lipid accumulation through transcription factor EB (TFEB)-regulated lipophagy
AbstractTacrolimus (TAC), also called FK506, is one of the classical immunosuppressants to prevent allograft rejection after liver transplantation. However, it has been proved to be associated with post-transplant hyperlipemia. The mechanism behind this is unknown, and it is urgent to explore preventive strategies for hyperlipemia after transplantation. Therefore, we established a hyperlipemia mouse model to investigate the mechanism, by injecting TAC intraperitoneally for eight weeks. After TAC treatment, the mice developed hyperlipemia (manifested as elevated triglyceride (TG) and low-density lipoprotein cholesterol (LDL...
Source: Journal of Zhejiang University. Science. B. - June 1, 2023 Category: Universities & Medical Training Source Type: research

Evolution of the newest diagnostic methods for COVID-19: a Chinese perspective
AbstractCoronavirus disease 2019 (COVID-19) has continued to spread globally since late 2019, representing a formidable challenge to the world ’s healthcare systems, wreaking havoc, and spreading rapidly through human contact. With fever, fatigue, and a persistent dry cough being the hallmark symptoms, this disease threatened to destabilize the delicate balance of our global community. Rapid and accurate diagnosis of COVID-19 is a prereq uisite for understanding the number of confirmed cases in the world or a region, and an important factor in epidemic assessment and the development of control measures. It also plays a c...
Source: Journal of Zhejiang University. Science. B. - June 1, 2023 Category: Universities & Medical Training Source Type: research

Cerebral ischemic injury after transcatheter aortic valve replacement in patients with pure aortic regurgitation
摘要目的本研究旨在比较重度主动脉瓣狭窄(AS)和单纯主动脉瓣反流(AR)患者接受经导管主动脉瓣置换术(TAVR)后发生脑损伤的风险.方法回顾性分析287例AS和65例单纯AR接受TAVR的患者. 患者在TAVR术前和术后3天进行头颅磁共振检查.结果本研究中57.7%为男性; 单纯AR患者年龄较小(71岁vs.74岁;P=0.001), 美国心胸外科医师协会评分较低(2.39 vs. 3.89;P<0.001); 3.1% 的患者在出院前发生了症状性脑卒中; 单纯AR组脑损伤病灶发生率(83.1% vs. 85.0%;P=0.695), 病灶数(3.0 (1.5‒7.5) vs. 3.0 (1.0‒8.0);P=0.928) 和总体...
Source: Journal of Zhejiang University. Science. B. - June 1, 2023 Category: Universities & Medical Training Source Type: research

In vivo antioxidant activity of rabbiteye blueberry (Vaccinium ashei cv. ‘Brightwell’) anthocyanin extracts
AbstractBlueberries are rich in phenolic compounds including anthocyanins which are closely related to biological health functions. The purpose of this study was to investigate the antioxidant activity of blueberry anthocyanins extracted from ‘Brightwell’ rabbiteye blueberries in mice. After one week of adaptation, C57BL/6J healthy male mice were divided into different groups that were administered with 100, 400, or 800 mg/kg blueberry anthocyanin extract (BAE), and sacrificed at different time points (0.1, 0.5, 1, 2, 4, 8, or 12 h). The plasma, eyeball, intestine, liver, and adipose tissues were collected to compare t...
Source: Journal of Zhejiang University. Science. B. - May 25, 2023 Category: Universities & Medical Training Source Type: research

Exploring the links between gut microbiome changes and irritable bowel syndrome in Han populations in the Tibetan Plateau
AbstractThe gut microbiome shows changes under a plateau environment, while the disbalance of intestinal microbiota plays an important role in the pathogenesis of irritable bowel syndrome (IBS); however, the relationship between the two remains unexplored. In this work, we followed up a healthy cohort for up to a year before and after living in a plateau environment and performed 16S ribosomal RNA (rRNA) sequencing analysis of their fecal samples. Through evaluating the participants ’ clinical symptoms, combined with an IBS questionnaire, we screened the IBS sub-population in our cohort. The sequencing results showed tha...
Source: Journal of Zhejiang University. Science. B. - May 23, 2023 Category: Universities & Medical Training Source Type: research

Biomarkers of malignant transformation in oral leukoplakia: from bench to bedside
AbstractOral leukoplakia is a common precursor lesion of oral squamous cell carcinoma, which indicates a high potential of malignancy. The malignant transformation of oral leukoplakia seriously affects patient survival and quality of life; however, it is difficult to identify oral leukoplakia patients who will develop carcinoma because no biomarker exists to predict malignant transformation for effective clinical management. As a major problem in the field of head and neck pathologies, it is imperative to identify biomarkers of malignant transformation in oral leukoplakia. In this review, we discuss the potential biomarker...
Source: Journal of Zhejiang University. Science. B. - May 13, 2023 Category: Universities & Medical Training Source Type: research

Multiplexed single-cell transcriptome analysis reveals molecular characteristics of monkey pluripotent stem cell lines
AbstractEfforts have been made to establish various human pluripotent stem cell lines. However, such methods have not yet been duplicated in non-human primate cells. Here, we introduce a multiplexed single-cell sequencing technique to profile the molecular features of monkey pluripotent stem cells in published culture conditions. The results demonstrate suboptimized maintenance of pluripotency and show that the selected signaling pathways for resetting human stem cells can also be interpreted for establishing monkey cell lines. Overall, this work legitimates the translation of novel human cell line culture conditions to mo...
Source: Journal of Zhejiang University. Science. B. - May 1, 2023 Category: Universities & Medical Training Source Type: research

CUDC-101 as a dual-target inhibitor of EGFR and HDAC enhances the anti-myeloma effects of bortezomib by regulating G2/M cell cycle arrest
AbstractCUDC-101, an effective and multi-target inhibitor of epidermal growth factor receptor (EGFR), histone deacetylase (HDAC), and human epidermal growth factor receptor 2 (HER2), has been reported to inhibit many kinds of cancers, such as acute promyelocytic leukemia and non-Hodgkin ’s lymphoma. However, no studies have yet investigated whether CUDC-101 is effective against myeloma. Herein, we proved that CUDC-101 effectively inhibits the proliferation of multiple myeloma (MM) cell lines and induces cell apoptosis in a time- and dose-dependent manner. Moreover, CUDC-101 marke dly blocked the signaling pathway of EGFR...
Source: Journal of Zhejiang University. Science. B. - May 1, 2023 Category: Universities & Medical Training Source Type: research