Journal of Zhejiang University. Science. B. This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Molecular cloning, characterization and expression analysis of three key starch synthesis-related genes from the bulb of a rare lily germplasm, Lilium brownii var. giganteum
In this study, we demonstrate thatLilium brownii var.giganteum, a wild lily with a giant bulb in nature, andL. brownii, the native species, have different starch levels and characteristics according to cytological and ultra-structural observations. We cloned the complete sequence of three key gene-encoding enzymes (LbgAGPS, LbgGBSS, and LbgSSIII) during starch synthesis by rapid amplification of 5 ′ and 3′ complementary DNA (cDNA) ends (RACE) technology. Bioinformatics analysis revealed that the proteins deduced by these genes contain the canonical conserved domains. Constructed phylogenetic trees confirmed the evoluti...
Source: Journal of Zhejiang University. Science. B. - June 1, 2021 Category: Universities & Medical Training Source Type: research

Protective effect of recombinant Lactobacillus plantarum against H2O2-induced oxidative stress in HUVEC cells
This study probed the protective effect of recombinantLactobacillus plantarum against hydrogen peroxide (H2O2)-induced oxidative stress in human umbilical vein endothelial cells (HUVECs). We constructed a new functionalL. plantarum (NC8-pSIP409-alr-angiotensin-converting enzyme inhibitory peptide (ACEIP)) with a double-gene-labeled non-resistant screen as an expression vector. A 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) colorimetric assay was carried out to determine the cell viability of HUVEC cells following pretreatment with NC8-pSIP409-alr-ACEIP. Flow cytometry (FCM) was used to determine t...
Source: Journal of Zhejiang University. Science. B. - May 1, 2021 Category: Universities & Medical Training Source Type: research

Development of a pyrF-based counterselectable system for targeted gene deletion in Streptomyces rimosus
In this study, we developed a screening system for targeted gene knockout using a uracil auxotrophic host ( ΔpyrF) resistant to the highly toxic uracil analog of 5-fluoroorotic acid (5-FOA) converted by PyrF, and a non-replicative vector pKC1132-pyrF carrying the complementedpyrF gene coding for orotidine-5 ′-phosphate decarboxylase. ThepyrF gene acts as a positive selection and counterselection marker for recombinants during genetic modifications. Single-crossover homologous integration mutants were selected on minimal medium without uracil by reintroducingpyrF along with pKC1132-pyrF into the genome of the mutant Δpy...
Source: Journal of Zhejiang University. Science. B. - May 1, 2021 Category: Universities & Medical Training Source Type: research

Genome-wide analysis of microRNA156 and its targets, the genes encoding SQUAMOSA promoter-binding protein-like (SPL) transcription factors, in the grass family Poaceae
AbstractMicroRNAs (miRNAs) are endogenous small non-coding RNAs that play an important role in post-transcriptional gene regulation in plants and animals by targeting messenger RNAs (mRNAs) for cleavage or repressing translation of specific mRNAs. The first miRNA identified in plants, miRNA156 (miR156), targets the SQUAMOSA promoter-binding protein-like (SPL) transcription factors, which play critical roles in plant phase transition, flower and plant architecture, and fruit development. We identified multiple copies ofMIR156 andSPL in the rice,Brachypodium, sorghum, maize, and foxtail millet genomes. Sequence and chromosom...
Source: Journal of Zhejiang University. Science. B. - May 1, 2021 Category: Universities & Medical Training Source Type: research

Phenolic profile of jujube fruit subjected to gut microbiota fermentation and its antioxidant potential against ethyl carbamate-induced oxidative damage
ConclusionsFermented jujube fruit extracts produced by GMF still contain biologically active substances which retain biological activity and antioxidation capabilities. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - May 1, 2021 Category: Universities & Medical Training Source Type: research

Exosomes in bladder cancer: novel biomarkers and targets
AbstractExosomes are nanometer-sized vesicles that contain various types of biologically active components, including proteins, nucleic acids, carbohydrates, and lipids, which vary with the type and physiological state of the cell. In recent years, several studies have showed that exosomes can provide new non-invasive diagnostic and prognostic biomarkers in patients affected by cancers, including bladder cancer (BC), and the lipid bilayer membrane structure makes exosomes as promising delivery vehicles for therapeutic applications. Exosomes have the characteristics of high abundance, high stability, tissue specificity, and...
Source: Journal of Zhejiang University. Science. B. - May 1, 2021 Category: Universities & Medical Training Source Type: research

Osteogenic effects of antihypertensive drug benidipine on mouse MC3T3-E1 cells in vitro
AbstractHypertension is a prevalent systemic disease in the elderly, who can suffer from several pathological skeletal conditions simultaneously, including osteoporosis. Benidipine (BD), which is widely used to treat hypertension, has been proved to have a beneficial effect on bone metabolism. In order to confirm the osteogenic effects of BD, we investigated its osteogenic function using mouse MC3T3-E1 preosteoblast cells in vitro. The proliferative ability of MC3T3-E1 cells was significantly associated with the concentration of BD, as measured by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and cell cycle assay...
Source: Journal of Zhejiang University. Science. B. - May 1, 2021 Category: Universities & Medical Training Source Type: research

Cornuside alleviates experimental autoimmune encephalomyelitis by inhibiting Th17 cell infiltration into the central nervous system
AbstractThe present study was conducted to clarify the therapeutic effect of cornuside on experimental autoimmune encephalomyelitis (EAE) and its influence on T helper 17 (Th17) cell and regulatory T (Treg) cell infiltration into the central nervous system. Rats were randomly placed into four treatment groups: control, EAE, EAE+cornuside, and EAE+prednisolone. The neurological function scores of rats were assessed daily. On the second day after EAE rats began to show neurological deficit symptoms, the four groups were treated with normal saline, normal saline, cornuside (150 mg/kg), and prednisolone (5 mg/kg), respectively...
Source: Journal of Zhejiang University. Science. B. - May 1, 2021 Category: Universities & Medical Training Source Type: research

Protective effect of recombinant Lactobacillus plantarum against H 2 O 2 -induced oxidative stress in HUVEC cells
This study probed the protective effect of recombinantLactobacillus plantarum against hydrogen peroxide (H2O2)-induced oxidative stress in human umbilical vein endothelial cells (HUVECs). We constructed a new functionalL. plantarum (NC8-pSIP409-alr-angiotensin-converting enzyme inhibitory peptide (ACEIP)) with a double-gene-labeled non-resistant screen as an expression vector. A 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) colorimetric assay was carried out to determine the cell viability of HUVEC cells following pretreatment with NC8-pSIP409-alr-ACEIP. Flow cytometry (FCM) was used to determine t...
Source: Journal of Zhejiang University. Science. B. - May 1, 2021 Category: Universities & Medical Training Source Type: research

Development of a pyrF -based counterselectable system for targeted gene deletion in Streptomyces rimosus
In this study, we developed a screening system for targeted gene knockout using a uracil auxotrophic host ( ΔpyrF) resistant to the highly toxic uracil analog of 5-fluoroorotic acid (5-FOA) converted by PyrF, and a non-replicative vector pKC1132-pyrF carrying the complementedpyrF gene coding for orotidine-5 ′-phosphate decarboxylase. ThepyrF gene acts as a positive selection and counterselection marker for recombinants during genetic modifications. Single-crossover homologous integration mutants were selected on minimal medium without uracil by reintroducingpyrF along with pKC1132-pyrF into the genome of the mutant Δpy...
Source: Journal of Zhejiang University. Science. B. - May 1, 2021 Category: Universities & Medical Training Source Type: research

Structural and physiological changes of the human body upon SARS-CoV-2 infection
AbstractSince December 2019, the novel Coronavirus (severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2)) has spread to many countries around the world, developing into a global pandemic with increasing numbers of deaths reported worldwide. To data, although some vaccines have been developed, there are no ideal drugs to treat novel coronavirus pneumonia (coronavirus disease 2019 (COVID-19)). By examining the structure of the coronavirus and briefly describing its possible pathogenesis based on recent autopsy reports conducted by various teams worldwide, this review analyzes the possible structural and functional ch...
Source: Journal of Zhejiang University. Science. B. - April 1, 2021 Category: Universities & Medical Training Source Type: research

High-throughput sequencing identifies salivary microbiota in Chinese caries-free preschool children with primary dentition
ConclusionsOur results revealed the diversity and composition of salivary microbiota in caries-free preschool children, with little difference between male and female subjects. Identity of the core microbiome, coupled with prediction of gene function, deepens our understanding of oral microbiota in caries-free populations and provides basic information for associating salivary microecology and oral health. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - April 1, 2021 Category: Universities & Medical Training Source Type: research

CRISPR/Cas: a Nobel Prize award-winning precise genome editing technology for gene therapy and crop improvement
AbstractSince it was first recognized in bacteria and archaea as a mechanism for innate viral immunity in the early 2010s, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) has rapidly been developed into a robust, multifunctional genome editing tool with many uses. Following the discovery of the initial CRISPR/Cas-based system, the technology has been advanced to facilitate a multitude of different functions. These include development as a base editor, prime editor, epigenetic editor, and CRISPR interference (CRISPRi) and CRISPR activator (CRISPRa) gene regulators. It can a...
Source: Journal of Zhejiang University. Science. B. - April 1, 2021 Category: Universities & Medical Training Source Type: research

A tetravalent single-chain variable fragment antibody for the detection of staphylococcal enterotoxin A
摘要免疫学方法可以用于金黄色葡萄球菌A型肠毒素的准确而快速的检测, 而快速生产高亲和力抗体是其中的关键步骤。在本研究中, 我们通过p53的四聚化功能结构域, 在金黄色葡萄球菌A型肠毒素特异性单链抗体的基础上, 成功制备了四 聚化的单链抗体。酶联免疫吸附试验表明该四聚化抗体对金黄色葡萄球菌A型肠毒素的亲和力显著高于其单体形式, 表明基于基因工程的四聚化是提高单链抗体亲和力的一种潜在的可行方法。 (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - April 1, 2021 Category: Universities & Medical Training Source Type: research

Effects of angiotensin II receptor blocker usage on viral load, antibody dynamics, and transcriptional characteristics among COVID-19 patients with hypertension
AbstractEpidemiological evidence suggests that patients with hypertension infected with severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) are at increased risk of acute lung injury. However, it is still not clear whether this increased risk is related to the usage of renin-angiotensin system (RAS) blockers. We collected medical records of coronavirus disease 2019 (COVID-19) patients from the First Affiliated Hospital, Zhejiang University School of Medicine (Hangzhou, China), and evaluated the potential impact of an angiotensin II receptor blocker (ARB) on the clinical outcomes of COVID-19 patients with hypertens...
Source: Journal of Zhejiang University. Science. B. - April 1, 2021 Category: Universities & Medical Training Source Type: research