Understanding and reducing the fear of COVID-19
(Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - June 18, 2020 Category: Universities & Medical Training Source Type: research

Erratum to: Transmission risk of patients with COVID-19 meeting discharge criteria should be interpreted with caution
The original version of this article unfortunately contained a mistake. For Fig. 1a in p.409, the citation of a reference, as well as the permission to reprint this picture, was missing. The correct version and the corresponding reference are given below:(a) Chest computed tomography (CT) image of Patient 1 on admission presents multiple ground-glass opacities distributed in the periphery of inferior lobe of both lungs. Reprinted from Zhang et al. (2020), with kind permission from Springer Nature. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - June 18, 2020 Category: Universities & Medical Training Source Type: research

Effect of resveratrol treatment on apoptosis and apoptotic pathways during boar semen freezing
AbstractResveratrol (3,5,4'-trihydroxystilbene, RSV) has been widely used in mammalian cells, but whether it can be used during freezing boar semen is still unknown. The effects of RSV treatment during boar semen freezing on its anti-freezing ability, apoptosis, and possible apoptotic pathways were observed in this study. Sperm motility, mitochondrial membrane potential ( ΔΨm), adenosine triphosphate (ATP) content, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL)-positive apoptotic state, and messenger RNA (mRNA) expression levels of apoptotic genes involved in different apoptot...
Source: Journal of Zhejiang University. Science. B. - June 1, 2020 Category: Universities & Medical Training Source Type: research

Effects of supplying silicon nutrient on utilization rate of nitrogen and phosphorus nutrients by rice and its soil ecological mechanism in a hybrid rice double-cropping system
This study was conducted to reveal the effects of silicon (Si) application on nutrient utilization efficiency by rice and on soil nutrient availability and soil microorganisms in a hybrid rice double-cropping planting system. A series of field experiments were conducted during 2017 and 2018. The results showed that Si nutrient supply improved grain yield and the utilization rates of nitrogen (N) and phosphorus (P) to an appropriate level for both early and late plantings, reaching a maximum at 23.4 kg/ha Si. The same trends were found for the ratios of available N (AN) to total N (TN) and available P (AP) to total P (TP), ...
Source: Journal of Zhejiang University. Science. B. - June 1, 2020 Category: Universities & Medical Training Source Type: research

Screening of miRNAs associated with lymph node metastasis in Her-2-positive breast cancer and their relationship with prognosis
AbstractThe aim of this study was to identify some biomarkers for predicting lymph node metastasis and prognosis of human epidermal growth factor receptor 2 (Her-2)-positive breast cancer (BC). We analyzed correlations between microRNAs (miRNAs) and the prognosis of patients with BC based on data collected from The Cancer Genome Atlas (TCGA) database. The expression levels of miR-455, miR-143, and miR-99a were measured in clinical samples of Her-2-positive BC patients with different degrees of lymph node metastasis. We investigated the impacts of overexpressed miR-455 on the proliferation and invasiveness of MDA-MB-453 cel...
Source: Journal of Zhejiang University. Science. B. - June 1, 2020 Category: Universities & Medical Training Source Type: research

Advances in studies on ion transporters involved in salt tolerance and breeding crop cultivars with high salt tolerance
AbstractSoil salinity is a global major abiotic stress threatening crop productivity. In salty conditions, plants may suffer from osmotic, ionic, and oxidative stresses, resulting in inhibition of growth and development. To deal with these stresses, plants have developed a series of tolerance mechanisms, including osmotic adjustment through accumulating compatible solutes in the cytoplasm, reactive oxygen species (ROS) scavenging through enhancing the activity of anti-oxidative enzymes, and Na+/K+ homeostasis regulation through controlling Na+ uptake and transportation. In this review, recent advances in studies of the mec...
Source: Journal of Zhejiang University. Science. B. - June 1, 2020 Category: Universities & Medical Training Source Type: research

Breeding for low cadmium accumulation cereals
AbstractCadmium (Cd) is an element that is nonessential and extremely toxic to both plants and human beings. Soil contaminated with Cd has adverse impacts on crop yields and threatens human health via the food chain. Cultivation of low-Cd cultivars has been of particular interest and is one of the most cost-effective and promising approaches to minimize human dietary intake of Cd. Low-Cd crop cultivars should meet particular criteria, including acceptable yield and quality, and their edible parts should have Cd concentrations below maximum permissible concentrations for safe consumption, even when grown in Cd-contaminated ...
Source: Journal of Zhejiang University. Science. B. - June 1, 2020 Category: Universities & Medical Training Source Type: research

Gene editing: an instrument for practical application of gene biology to plant breeding
AbstractPlant breeding is well recognized as one of the most important means to meet food security challenges caused by the ever-increasing world population. During the past three decades, plant breeding has been empowered by both new knowledge on trait development and regulation (e.g., functional genomics) and new technologies (e.g., biotechnologies and phenomics). Gene editing, particularly by clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) and its variants, has become a powerful technology in plant research and may become a game-changer in plant breeding. Traits are con...
Source: Journal of Zhejiang University. Science. B. - June 1, 2020 Category: Universities & Medical Training Source Type: research

Breeding crops by design for future agriculture
(Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - June 1, 2020 Category: Universities & Medical Training Source Type: research

Initial chest CT findings in COVID-19: correlation with clinical features
中文概要目的分析2019 冠状病毒病 (COVID-19) 患者首次胸部 CT 影像表现及其与临床表现 (包括发热症状、实验室检查) 的相关性。创新点初步探索 COVID-19 肺部病灶与临床数据的相关性, 对评估 COVID-19 患者提供一定的依据。方法纳入 2020 年 1 月 16 日至 2020 年 2 月 6 日入院的 36 例 COVID-19 患者, 回顾性分析首次胸部 CT 的影像学表现, 包括影像特征、分布模式、累及 范围等, 并进一步分析其与临床特征 (包括发病 时间、发热症状、白细胞计数等) 的相关性。结论结果发现, 在自然病程...
Source: Journal of Zhejiang University. Science. B. - May 20, 2020 Category: Universities & Medical Training Source Type: research

Comparison of epidemiological and clinical characteristics of COVID-19 patients with and without Wuhan exposure
This study investigates the epidemiological and clinical characteristics of coronavirus disease 2019 (COVID-19) patients in Zhejiang Province who did or did not have a history of Wuhan exposure.MethodsWe collected data from medical records of confirmed COVID-19 patients in Zhejiang Province from Jan. 17 to Feb. 7, 2020 and analyzed epidemiological, clinical, and treatment data of those with and without recorded recent exposure in Wuhan.ResultsPatients in the control group were older than those in the exposure group ((48.19 ±16.13) years vs. (43.47±13.12) years,P
Source: Journal of Zhejiang University. Science. B. - May 9, 2020 Category: Universities & Medical Training Source Type: research

Primary percutaneous coronary intervention in a COVID-19 patient with ST-segment elevation myocardial infarction after lung transplantation: a case report
概要本文首次报告了重症新型冠状病毒肺炎(COVID-19)感染接受肺移植并行急症冠脉介入治疗的病 例。通过对多学科协作成功救治的总结, 为重症 COVID-19 救治提供经验。我们发现, 重症 COVID-19 感染在肺移植术后可能出现多种危险 因素诱 发心肌梗死, 急症冠脉介入治疗是救治此 类患者的重要手段。 (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - May 9, 2020 Category: Universities & Medical Training Source Type: research

Analysis of an improved workflow of endoscope reprocessing for bedside endoscopic diagnosis and treatment on COVID-19 patients
AbstractSevere cases infected with the coronavirus disease 2019 (COVID-19), named by the World Health Organization (WHO) on Feb. 11, 2020, tend to present a hypercatabolic state because of severe systemic consumption, and are susceptible to stress ulcers and even life-threatening gastrointestinal bleeding. Endoscopic diagnosis and treatment constitute an irreplaceable part in the handling of severe COVID-19 cases. Endoscopes, as reusable precision instruments with complicated structures, require more techniques than other medical devices in cleaning, disinfection, sterilization, and other reprocessing procedures. From 2016...
Source: Journal of Zhejiang University. Science. B. - May 9, 2020 Category: Universities & Medical Training Source Type: research

Can SARS-CoV-2-infected women breastfeed after viral clearance?
概 要浙江大学医学院附属第一医院感染科收治一名新型冠状病毒 (SARS-CoV-2) 感染的妊娠晚期孕妇. 在其住院期间, 患者进行了剖腹产术, 新生儿被证实无 SARS-CoV-2 感染. 结合已有的相关文献报道, SARS-CoV-2 通过母婴垂直传播的依据不足, 同 时乳汁的病毒检测阴性, 因此目前不能证明 SARS-CoV-2 病毒能通过乳汁传播. 通过研究, 我们建议感染 SARS-CoV-2 的孕妇, 在治愈出院后, 应经过两周隔离观察, 并且病毒复查结果为阴性, 才可以进行母乳喂养. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - May 8, 2020 Category: Universities & Medical Training Source Type: research

An overview of COVID-19
AbstractPneumonia caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection emerged in Wuhan City, Hubei Province, China in December 2019. By Feb. 11, 2020, the World Health Organization (WHO) officially named the disease resulting from infection with SARS-CoV-2 as coronavirus disease 2019 (COVID-19). COVID-19 represents a spectrum of clinical manifestations that typically include fever, dry cough, and fatigue, often with pulmonary involvement. SARS-CoV-2 is highly contagious and most individuals within the population at large are susceptible to infection. Wild animal hosts and infected patients are ...
Source: Journal of Zhejiang University. Science. B. - May 8, 2020 Category: Universities & Medical Training Source Type: research

Transmission risk of patients with COVID-19 meeting discharge criteria should be interpreted with caution
概 要目前全球新型冠状病毒流行形势仍严峻且持续恶化. 评估符合出院标准的 2019 冠状病毒病 (COVID-19) 患者的传染性对于疾病控制至关重要. 本研究报告两例核酸检测结果与临床表现及影像学表现不相符的患者, 以此来呼吁临床工作者 谨慎考虑符合出院标准的 COVID-19 患者的传染性. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - May 8, 2020 Category: Universities & Medical Training Source Type: research

Clinical characteristics of 34 COVID-19 patients admitted to intensive care unit in Hangzhou, China
ConclusionsData showed that the rates of complications, dynamics of lymphocytopenia, and changes in levels of platelet, hemoglobin, BUN, D-dimer, LDH and IL-6, and prothrombin time in these ICU patients were significantly different between IMV and NIV cases. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - May 1, 2020 Category: Universities & Medical Training Source Type: research

Effects of leptin-modified human placenta-derived mesenchymal stem cells on angiogenic potential and peripheral inflammation of human umbilical vein endothelial cells (HUVECs) after X-ray radiation
AbstractCombined radiation-wound injury (CRWI) is characterized by blood vessel damage and pro-inflammatory cytokine deficiency. Studies have identified that the direct application of leptin plays a significant role in angiogenesis and inflammation. We established a sustained and stable leptin expression system to study the mechanism. A lentivirus method was employed to explore the angiogenic potential and peripheral inflammation of irradiated human umbilical vein endothelial cells (HUVECs).Leptin was transfected into human placenta-derived mesenchymal stem cells (HPMSCs) with lentiviral vectors. HUVECs were irradiated by ...
Source: Journal of Zhejiang University. Science. B. - April 1, 2020 Category: Universities & Medical Training Source Type: research

Cyclooxygenase-2 promotes ovarian cancer cell migration and cisplatin resistance via regulating epithelial mesenchymal transition
ConclusionsCOX-2 promotes cancer cell migration and CDDP resistance and may serve as a potential target for curing OC. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - April 1, 2020 Category: Universities & Medical Training Source Type: research

Central visual function and inner retinal structure in primary open-angle glaucoma
This study enrolled 78 POAG patients and 58 healthy controls. POAG was classified into early glaucoma and moderate to advanced glaucoma. The following tests were performed on all participants: isolated-check visual evoked potential (icVEP) testing, 24 –2 standard automated perimetry (SAP), and Cirrus optical coherence tomography (OCT) examinations. Signal-to-noise ratio (SNR) measures obtained from icVEP responses to isolated checks presented at four depths of modulation (DOMs; 8%, 14%, 22%, and 32%) were explored. Mean macular sensitivity (mMS ) was assessed by calculating the mean sensitivities of central 12 SAP po...
Source: Journal of Zhejiang University. Science. B. - April 1, 2020 Category: Universities & Medical Training Source Type: research

Effects of sulforaphane on brain mitochondria: mechanistic view and future directions
AbstractThe organosulfur compound sulforaphane (SFN; C6H11NOS2) is a potent cytoprotective agent promoting antioxidant, anti-inflammatory, antiglycative, and antimicrobial effects in in vitro and in vivo experimental models. Mitochondria are the major site of adenosine triphosphate (ATP) production due to the work of the oxidative phosphorylation (OXPHOS) system. They are also the main site of reactive oxygen species (ROS) production in nucleated human cells. Mitochondrial impairment is central in several human diseases, including neurodegeneration and metabolic disorders. In this paper, we describe and discuss the effects...
Source: Journal of Zhejiang University. Science. B. - April 1, 2020 Category: Universities & Medical Training Source Type: research

Retraction Note to: Effects of dietary glycerol monolaurate on productive performance, egg quality, serum biochemical indices, and intestinal morphology of laying hens
The authors have retracted this article (Zhao et al., 2019) due to significant overlap with a previously published Chinese language article (Liu et al., 2017), including overlap in Table 1, Table 2, Table 3, Table 6, Fig. 4, and part of the results (Sections 3.1, 3.2, 3.5, and 3.7).All authors agree with this retraction. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - April 1, 2020 Category: Universities & Medical Training Source Type: research

Efficacy of internet-based integrated intervention on depression and anxiety symptoms in patients with COVID-19
中文概要目的目的探讨基于网络的整合心理干预对新冠肺炎患者抑 郁焦虑症状的有效性。创新点创新点针对大规模公共卫生事件的患者, 首次采用基于 网络的整合心理干预技术开展抑郁焦虑症状的 治疗。这不仅能够让隔离患者更便捷地获得心理 干预, 而且减少了心理医生的感染暴露机会。方法方法纳入26 例伴有抑郁焦虑症状的新冠肺炎患者, 随机分为干预组和对照组。干预组给予基于网络 的整合心理干预, 患者在医生指导下, 通过智能 手机聆听音频材料, 并接受整合心理干...
Source: Journal of Zhejiang University. Science. B. - April 1, 2020 Category: Universities & Medical Training Source Type: research

Dialectical behavior therapy-based psychological intervention for woman in late pregnancy and early postpartum suffering from COVID-19: a case report
概要目 的孕产妇的心理健康风险较为突出, 但尚未有文献说明何种心理干预方式对患有新冠肺炎的围产期孕妇有效. 本文报告了一例确诊新冠肺炎的 35 周 2 天孕妇在入住隔离病房后, 使用辩证行为治疗 (DBT) 相关技术对其存在的抑郁和焦虑症 状在围产期进行持续心理干预的案例, 旨在探究以 DBT 为基础的短程心理治疗对围产期新冠肺炎患者的抑郁和焦虑症状的改善作用.创新点首例对确诊新冠肺炎的孕产妇进行产前及产后心理评估及干预的案例. 也是首次报道在公共卫生危机事...
Source: Journal of Zhejiang University. Science. B. - April 1, 2020 Category: Universities & Medical Training Source Type: research

Comparative transcriptomic analysis of vascular endothelial cells after hypoxia/re-oxygenation induction based on microarray technology
Conclusions: The data from this study have built a foundation to develop many hypotheses to further explore the hypoxia/re-oxygenation mechanisms, an area with great clinical significance for multiple diseases. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - March 22, 2020 Category: Universities & Medical Training Source Type: research

Use of Tripterygium wilfordii Hook F for immune-mediated inflammatory diseases: progress and future prospects
AbstractTripterygium wilfordii Hook F has significant anti-inflammatory and immunosuppressive properties and is widely used for treating autoimmune and inflammatory diseases such as rheumatoid arthritis, systemic lupus erythematosus, and kidney disease, especially in traditional Chinese medicine. The mechanisms underlying its effects may be diverse but they remain unclear, and its toxicity and side effects limit its wider clinical application. This review summarizes the clinical application ofTripterygium wilfordii Hook F in recent years, as well as the results of studies into its mechanisms and toxicity, to provide a refe...
Source: Journal of Zhejiang University. Science. B. - March 16, 2020 Category: Universities & Medical Training Source Type: research

Possible aerosol transmission of COVID-19 and special precautions in dentistry
AbstractSince its emergence in December 2019, corona virus disease 2019 (COVID-19) has impacted several countries, affecting more than 90 thousand patients and making it a global public threat. The routes of transmission are direct contact, and droplet and possible aerosol transmissions. Due to the unique nature of dentistry, most dental procedures generate significant amounts of droplets and aerosols, posing potential risks of infection transmission. Understanding the significance of aerosol transmission and its implications in dentistry can facilitate the identification and correction of negligence in daily dental practi...
Source: Journal of Zhejiang University. Science. B. - March 16, 2020 Category: Universities & Medical Training Source Type: research

Clinical value of the emergency department in screening and diagnosis of COVID-19 in China
概 要目 的通过对浙江大学医学院附属第一医院急诊科筛查出的 4 例新型冠状病毒肺炎 (新冠肺炎) 病例进行分析, 探讨急诊科在新冠肺炎精准筛查和诊断中的作用。创新点分析新型冠状病毒肺炎的致病机制、 传播方式和临床特征; 总结急诊科医护人员的自身防护和诊断策略.方 法回顾性分析我院急诊科筛查出的 4 例新型冠状病毒肺炎病例, 总结急诊科的临床价值.结 论在新冠肺炎的筛查和诊断体系中, 中国急诊科发挥了不可替代的作用. 急诊科医护人员应充分做好自身防护, 对...
Source: Journal of Zhejiang University. Science. B. - March 16, 2020 Category: Universities & Medical Training Source Type: research

Role of LINC00152 in non-small cell lung cancer
AbstractNon-small cell lung cancer (NSCLC) accounts for about 85% of all lung cancer cases. The pathogenesis of NSCLC involves complex gene networks that include different types of non-coding RNAs, such as long non-coding RNAs (lncRNAs). The role of lncRNAs in NSCLC is gaining an increasing interest as their function is being explored in various human cancers. Recently, a new oncogenic lncRNA, LINC00152 (cytoskeleton regulator RNA (CYTOR)), has been identified in different tumor types. In NSCLC, the high expression of LINC00152 in tumor tissue and peripheral blood samples has been shown to be associated with worse prognose...
Source: Journal of Zhejiang University. Science. B. - March 1, 2020 Category: Universities & Medical Training Source Type: research

Roles of pattern recognition receptors in diabetic nephropathy
AbstractDiabetic nephropathy (DN) is currently the most common complication of diabetes. It is considered to be one of the leading causes of end-stage renal disease (ESRD) and affects many diabetic patients. The pathogenesis of DN is extremely complex and has not yet been clarified; however, in recent years, increasing evidence has shown the important role of innate immunity in DN pathogenesis. Pattern recognition receptors (PRRs) are important components of the innate immune system and have a significant impact on the occurrence and development of DN. In this review, we classify PRRs into secretory, endocytic, and signal ...
Source: Journal of Zhejiang University. Science. B. - March 1, 2020 Category: Universities & Medical Training Source Type: research

Identification of PTPRR and JAG1 as key genes in castration-resistant prostate cancer by integrated bioinformatics methods
In conclusion,PTPRR andJAG1 are key genes in the CRPC, which may serve as promising biomarkers of diagnosis and prognosis of CRPC. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - March 1, 2020 Category: Universities & Medical Training Source Type: research

Primary age-related tauopathy in a Chinese cohort
摘要目的分析中国人群中原发性衰老相关性tau 病(PART)的临床病理学特点,并比较其与老年性痴呆之间的差异.创新点PART 为新近提出的概念,其与老年性痴呆的关 系尚存在争论.目前未见针对中国人群PART 的 相关报道.本文首次报道了浙江大学医学院中国 人脑库中的PART 的临床病理学特点.方法回顾性分析相关病例,严格纳入标准.PART 在 中国人群中常见,50 岁以上人群占47%.平均年 龄(76±12)岁,女性14 例(30%),男性32 例 (70%).免疫组织化学法显示,按磷酸化tau 的Braak 分期,PART 大多为I 到...
Source: Journal of Zhejiang University. Science. B. - March 1, 2020 Category: Universities & Medical Training Source Type: research

Inhibition of chemotherapy-related breast tumor EMT by application of redox-sensitive siRNA delivery system CSO-ss-SA/siRNA along with doxorubicin treatment
AbstractMetastasis is one of the main reasons causing death in cancer patients. It was reported that chemotherapy might induce metastasis. In order to uncover the mechanism of chemotherapy-induced metastasis and find solutions to inhibit treatment-induced metastasis, the relationship between epithelial-mesenchymal transition (EMT) and doxorubicin (DOX) treatment was investigated and a redox-sensitive small interfering RNA (siRNA) delivery system was designed. DOX-related reactive oxygen species (ROS) were found to be responsible for the invasiveness of tumor cells in vitro, causing enhanced EMT and cytoskeleton reconstruct...
Source: Journal of Zhejiang University. Science. B. - March 1, 2020 Category: Universities & Medical Training Source Type: research

Regulatory mechanisms and therapeutic potential of microglial inhibitors in neuropathic pain and morphine tolerance
AbstractMicroglia are important cells involved in the regulation of neuropathic pain (NPP) and morphine tolerance. Information on their plasticity and polarity has been elucidated after determining their physiological structure, but there is still much to learn about the role of this type of cell in NPP and morphine tolerance. Microglia mediate multiple functions in health and disease by controlling damage in the central nervous system (CNS) and endogenous immune responses to disease. Microglial activation can result in altered opioid system activity, and NPP is characterized by resistance to morphine. Here we investigate ...
Source: Journal of Zhejiang University. Science. B. - March 1, 2020 Category: Universities & Medical Training Source Type: research

Amyloid precursor protein regulates 5-fluorouracil resistance in human hepatocellular carcinoma cells by inhibiting the mitochondrial apoptotic pathway
AbstractHepatocellular carcinoma (HCC) is a malignant tumor with high morbidity and mortality globally. It accounts for the majority of primary liver cancer cases. Amyloid precursor protein (APP), a cell membrane protein, plays a vital role in the pathogenesis of Alzheimer ’s disease, and has been found to be implicated in tumor growth and metastasis. Therefore, to understand the relationship between APP and 5-fluorouracil (5-FU) resistance in liver cancer, Cell Counting Kit-8, apoptosis and cell cycle assays, western blotting, and reverse transcription-quantitative polymerase chain reaction (qPCR) analysis were perf...
Source: Journal of Zhejiang University. Science. B. - March 1, 2020 Category: Universities & Medical Training Source Type: research

Spinal P2X7R contributes to streptozotocin-induced mechanical allodynia in mice
AbstractPainful diabetic neuropathy (PDN) is a diabetes mellitus complication. Unfortunately, the mechanisms underlying PDN are still poorly understood. Adenosine triphosphate (ATP)-gated P2X7 receptor (P2X7R) plays a pivotal role in non-diabetic neuropathic pain, but little is known about its effects on streptozotocin (STZ)-induced peripheral neuropathy. Here, we explored whether spinal cord P2X7R was correlated with the generation of mechanical allodynia (MA) in STZ-induced type 1 diabetic neuropathy in mice. MA was assessed by measuring paw withdrawal thresholds and western blotting. Immunohistochemistry was applied to ...
Source: Journal of Zhejiang University. Science. B. - February 14, 2020 Category: Universities & Medical Training Source Type: research

CX3CR1 contributes to streptozotocin-induced mechanical allodynia in the mouse spinal cord
摘要目 的趋化因子 (CX3CL1) 在神经性疼痛中起重要的生理病理作用, 然而其在糖尿病神经病理痛中的作用还有待研究。 本实验主要研究了在糖尿病小鼠痛阈下调的时间窗内, 脊髓背角中趋化因子 CX3CL1/趋化因子受体 (CX3CR1) 在触诱发痛发生与 发展中的作用。创新点主要探讨 CX3CR1 在链脲佐菌素 (STZ) 诱导的 1 型糖尿病 (T1DM) 小鼠早期发生的机械痛性神经病变中的作用。方 法本实验采用健康雄性 C57BL/6 小鼠与 CX3CR1 KO 小鼠, 体重 20∼30 g, 隔夜禁食12 h (20 点至次日 8 点), 并连续三天...
Source: Journal of Zhejiang University. Science. B. - February 14, 2020 Category: Universities & Medical Training Source Type: research

Salinity tolerance in barley during germination —homologs and potential genes
AbstractSalinity affects more than 6% of the world ’s total land area, causing massive losses in crop yield. Salinity inhibits plant growth and development through osmotic and ionic stresses; however, some plants exhibit adaptations through osmotic regulation, exclusion, and translocation of accumulated Na+ or Cl-. Currently, there are no practical, economically viable methods for managing salinity, so the best practice is to grow crops with improved tolerance. Germination is the stage in a plant ’s life cycle most adversely affected by salinity. Barley, the fourth most important cereal crop in the world, has o...
Source: Journal of Zhejiang University. Science. B. - February 5, 2020 Category: Universities & Medical Training Source Type: research

Analysis of nicotine-induced metabolic changes in Blakeslea trispora by GC-MS
概要在三孢布拉霉发酵番茄红素过程中, 阻断剂是必不可少的组分. 尼古丁作为目前已知的最有效的番茄红素阻断剂之一, 其在工业生产上具有巨大的实用价值. 但阻断剂作为菌体的外源毒性生物碱, 其对菌体的代谢势必有所改变. 本文 以尼古丁作为阻断剂, 对其在三孢布拉霉发酵过程中的代谢影响做了初步研究. 基于气相色谱-质谱 (GC-MS) 检测技术及小分子代谢组分的主成分分析 (PCA), 证明尼古丁在三孢布拉霉菌体内严重抑制氨基酸及糖代谢, 同时脂肪酸和有机 酸代谢也有所减...
Source: Journal of Zhejiang University. Science. B. - February 5, 2020 Category: Universities & Medical Training Source Type: research

Roles of PTBP1 in alternative splicing, glycolysis, and oncogensis
AbstractPolypyrimidine tract-binding protein 1 (PTBP1) plays an essential role in splicing and is expressed in almost all cell types in humans, unlike the other proteins of the PTBP family. PTBP1 mediates several cellular processes in certain types of cells, including the growth and differentiation of neuronal cells and activation of immune cells. Its function is regulated by various molecules, including microRNAs (miRNAs), long non-coding RNAs (IncRNAs), and RNA-binding proteins. PTBP1 plays roles in various diseases, particularly in some cancers, including colorectal cancer, renal cell cancer, breast cancer, and glioma. ...
Source: Journal of Zhejiang University. Science. B. - February 5, 2020 Category: Universities & Medical Training Source Type: research

Preliminary study on the role of novel LysR family gene kp05372 in Klebsiella pneumoniae of forest musk deer
AbstractLysR-type transcriptional regulators are involved in the regulation of numerous cellular metabolic processes inKlebsiella pneumoniae, leading to severe infection. Earlier, we found a novel LysR family gene, namedkp05372, in a strain ofK. pneumoniae (designated GPKP) isolated from forest musk deer. To study the function of this gene in relation to the biological characteristics of GPKP, we used the suicide plasmid and conjugative transfer methods to construct deletion mutant strain GPKP- Δkp05372; moreover, we also constructed the GPKP- Δkp05372+ complemented strain. The role of this gene was determined ...
Source: Journal of Zhejiang University. Science. B. - February 5, 2020 Category: Universities & Medical Training Source Type: research

Toxicity and metabolism of 3-bromopyruvate in Caenorhabditis elegans
In this study, we aimed to evaluate the toxic effects, changes in life span, and expression of various metabolism-related genes inCaenorhabditis elegans, using RNA interference (RNAi) and mutant strains, after 3-bromopyruvate (3-BrPA) treatment.C. elegans was treated with various concentrations of 3-BrPA on nematode growth medium (NGM) plates, and their survival was monitored every 24 h. The expression of genes related to metabolism was measured by the real-time fluorescent quantitative polymerase chain reaction (qPCR). Nematode survival in the presence of 3-BrPA was also studied after silencing three hexokinase (HK) genes...
Source: Journal of Zhejiang University. Science. B. - January 1, 2020 Category: Universities & Medical Training Source Type: research

Lymphatic vessels, miRNAs, and CAR T cells in tumor immunology
(Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - January 1, 2020 Category: Universities & Medical Training Source Type: research

Erratum to: Exosome-derived galectin-9 may be a novel predictor of rejection and prognosis after liver transplantation
The original version of this article unfortunately contained a mistake.In p.605, the number of the Zhejiang Provincial Natural Science Foundation of China (No. Y17H160118) in Funding is incorrect. The correct number should be LY17H160026, which is the approval number of the project, whereas Y17H160118 is the application number of the project. (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - December 21, 2019 Category: Universities & Medical Training Source Type: research

Erratum to: Catalpol ameliorates LPS-induced endometritis by inhibiting inflammation and TLR4/NF- κB signaling
The original version of this article unfortunately contained a mistake. In p.823, Figs. 8c and 8d were incorrect, and the obvious pathological changes were mistakenly placed in the picture. The correct versions should be as follows: (Source: Journal of Zhejiang University. Science. B.)
Source: Journal of Zhejiang University. Science. B. - December 21, 2019 Category: Universities & Medical Training Source Type: research

MicroRNAs in tumor immunity: functional regulation in tumor-associated macrophages
AbstractTumor-associated macrophages (TAMs) are the most abundant immune cells in the tumor microenvironment (TME) and are critical for cancer initiation and progression. MicroRNAs (miRNAs) could notably influence the phenotype of TAMs through various targets and signal pathways during cancer progression due to their post-transcriptional regulation. In this review, we discuss mainly the regulatory function of miRNAs on macrophage differentiation, functional polarization, and cellular crosstalk. Firstly, during the generation process, miRNAs take part in the differentiation from myeloid cells to mature macrophages, and this...
Source: Journal of Zhejiang University. Science. B. - December 21, 2019 Category: Universities & Medical Training Source Type: research

Current advances in chimeric antigen receptor T-cell therapy for refractory/relapsed multiple myeloma
Abstractmultiple myeloma (MM), considered an incurable hematological malignancy, is characterized by its clonal evolution of malignant plasma cells. Although the application of autologous stem cell transplantation (ASCT) and the introduction of novel agents such as immunomodulatory drugs (IMiDs) and proteasome inhibitors (PIs) have doubled the median overall survival to eight years, relapsed and refractory diseases are still frequent events in the course of MM. To achieve a durable and deep remission, immunotherapy modalities have been developed for relapsed/refractory multiple myeloma (RRMM). Among these approaches, chime...
Source: Journal of Zhejiang University. Science. B. - December 21, 2019 Category: Universities & Medical Training Source Type: research

Carfilzomib inhibits the growth of lung adenocarcinoma via upregulation of Gadd45a expression
In this study, we have systematically investigated the CFZ functions in cell proliferation and growth, cell cycle arrest, and apoptosis in lung adenocarcinoma cells. Flow cytometry experiments showed that CFZ significantly induced G2/M cell cycle arrest and apoptosis in lung adenocarcinoma. MTS and colony formation assays revealed that CFZ substantially inhibited survival of lung adenocarcinoma cells. All results were consistently correlated to the upregulation expression ofGadd45a, which is an important gene in modulating cell cycle arrest and apoptosis in response to physiologic and environmental stresses. Here, upregula...
Source: Journal of Zhejiang University. Science. B. - December 21, 2019 Category: Universities & Medical Training Source Type: research

Psychophysiological and cognitive effects of strawberry plants on people in isolated environments
This study further explored the impact of viewing strawberry plants on cognition. A notable re duction of the subjects’ reaction time after 15-min plant viewing was observed. Based on these findings, a long-duration isolated experiment in a bioregenerative life support system—“Lunar Palace I”—was conducted. A similar trend was obtained that crew members’ mood states were improved by viewing the strawberry plants, but no significant change was observed. This study provided some experimental evidence for the benefits of interacting with strawberry plants in isolated environments. (Source: ...
Source: Journal of Zhejiang University. Science. B. - December 21, 2019 Category: Universities & Medical Training Source Type: research

Relationships between blood leukocyte mitochondrial DNA copy number and inflammatory cytokines in knee osteoarthritis
AbstractOsteoarthritis (OA) is a degenerative articular disorder manifested by cartilage destruction, subchondral sclerosis, osteophytes, and synovitis, resulting in chronic joint pain and physical disability in the elderly. The purpose of this study was to investigate mitochondrial DNA copy number (mtDNACN) and inflammatory cytokines in primary knee OA patients and healthy volunteers. A total of 204 knee OA patients and 169 age-matched healthy volunteers were recruited. Their relative blood leukocyte mtDNACN was assessed by quantitative real-time polymerase chain reaction (qRT-PCR), and ten inflammatory cytokines in their...
Source: Journal of Zhejiang University. Science. B. - December 21, 2019 Category: Universities & Medical Training Source Type: research