Heterotopic ossification in a patient with diffuse idiopathic skeletal hyperostosis: Input from histological findings
A high incidence of heterotopic ossification (HO) has been reported in patients with diffuse idiopathic skeletal hyperostosis (DISH), a metabolic disease characterized by calcifications of entheses at spine and peripheral sites. We performed histological and immunohistochemical analyses in five different HO sites in a patient with DISH to study a possible mutual interaction of bone morphogenetic protein 2 (BMP-2), transforming growth factor beta (TGF- β), and decorin, crucial for bone mass increasing, matrix calcification, and endochondral bone formation. We speculated that the surgical trauma triggered HO, indu...
Source: European Journal of Histochemistry - December 3, 2020 Category: Biomedical Science Authors: Caterina Licini, Luca Farinelli, Giorgia Cerqueni, Andrell Hosein, Saverio Marchi, Antonio Gigante, Monica Mattioli-Belmonte Source Type: research
Essential Current Concepts in Stem Cell Biology
Stem cell (SC) biology is a pervasive transdisciplinary research field encompassing any level of life organization (from molecular to morphological), combining different types of techniques (spanning from cellular to molecular)..... (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - November 18, 2020 Category: Biomedical Science Authors: Manuela Monti Source Type: research
Proceedings of the 30th National Conference of the Italian Group for the Study of Neuromorphology “Gruppo Italiano per lo Studio della Neuromorfologia” G.I.S.N. Torino, November 12-14, 2020
Proceedings of the 30th National Conference of the Italian Group for the Study of Neuromorphology “Gruppo Italiano per lo Studio della Neuromorfologia” G.I.S.N., November 12-14, 2020, University of Torino, Torino (Virtual Event) - Italy. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - November 16, 2020 Category: Biomedical Science Authors: Conference Chair: Giancarlo Panzica Source Type: research
Prognostic significance of CD133 and ABCB5 expression in papillary thyroid carcinoma
Expression of CD133 and ABCB5 is associated with tumor aggressiveness, but evidence in papillary thyroid cancer (PTC) is lacking. We correlated CD133 and ABCB5 expression with pathological characteristics and factors of worse prognosis in PTC. Samples of 119 PTCs and 40 controls (goiters) were distributed in 8 tissue microarray blocks and evaluated with immunohistochemistry using anti-CD133 and anti-ABCB5 antibodies. The expression of each marker alone and combined was analyzed against pathological characteristics and factors of worse prognosis in PTC. Expression of CD133 alone (19 tumors, 16.0%) was more frequent in patie...
Source: European Journal of Histochemistry - November 12, 2020 Category: Biomedical Science Authors: Maria Augusta Karas Zella, Ana Paula Martins Sebasti ão, Luiz Martins Collaço, Daniel Cury Ogata, Guilherme Cecchetti, Ivan José Paredes Bartolomei, Ana Maria Waaga-Gasser, Carmen Australia Paredes Marcondes Ribas Source Type: research
LncRNA GClnc1 may contribute to the progression of ovarian cancer by regulating p53 signaling pathway
In this study, we aimed to uncover the underlying molecular mechanism of long non-coding RNA (lncRNA) GClnc1 related to p53 signaling pathway in OC. The expression of lncRNA H19 GClnc1 was markedly higher in OC samples than the related normal tissues. Next, we found that lncRNA GClnc1 inhibited p53. In addition, the lncRNA GClnc1 overexpression promoted the cell proliferation and migrationin vitro. Subsequently, p53 silencing obligated the effect of lncRNA GCln1 knock down on cell proliferation and migration. To sum up, LncRNA GClnc1 contributes to the progression of OC by regulating p53 signaling pathway. Meanwhile, our f...
Source: European Journal of Histochemistry - November 10, 2020 Category: Biomedical Science Authors: Hu Li, Zheng Zeng, Xiang Yang, Ye Chen, Lei He, Ting Wan Source Type: research
The selective blockade of metabotropic glutamate receptor-5 attenuates fat accumulation in an < em > in vitro < /em > model of benign steatosis
In conclusion, mGluR5 pharmacological blockade reduced fat accumulation in HepG2 cel ls incubated with O/P, probably by modulating the expression of SREBP-1 and PPAR-α. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - October 19, 2020 Category: Biomedical Science Authors: Andrea Ferrigno, Clarissa Berardo, Laura Giuseppina Di Pasqua, Marta Cagna, Veronica Siciliano, Plinio Richelmi, Mariapia Vairetti Source Type: research
An emerging potential therapeutic target for osteoporosis: LncRNA H19/miR-29a-3p axis
This study might provide a better understanding of OP development and a potential therapeutic target for OP intervention. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - October 19, 2020 Category: Biomedical Science Authors: Ziqi Li, Zhinan Hong, Yuesheng Zheng, Yongwei Dong, Wei He, Yingjia Yuan, Junbiao Guo Source Type: research
miR-126 targeting GOLPH3 inhibits the epithelial-mesenchymal transition of gastric cancer BGC-823 cells and reduces cell invasion
This study may provide a new field of vision for targeted treatment of gastric cancer. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - October 19, 2020 Category: Biomedical Science Authors: Jiancai Ouyang, Fuhu Song, He Li, Rui Yang, Haicheng Huang Source Type: research
Ciliary neurotrophic factor (CNTF) and its receptor (CNTFR α) signal through MAPK/ERK pathway in human prostate tissues: a morphological and biomolecular study
In conclusion, we suggest that CNTF could be considered as key molecule to maintenance epithelium homeostasisvia pERK downregulation by an autocrine mechanism. Further CNTF studies in prostate cancer could be useful to verify the potential role of this cytokine in carcinogenesis. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - October 19, 2020 Category: Biomedical Science Authors: Sonia Fantone, Giovanni Tossetta, Rodolfo Montironi, Martina Senzacqua , Daniela Marzioni, Roberta Mazzucchelli Source Type: research
Swertiamarin suppresses proliferation, migration, and invasion of hepatocellular carcinoma cells < em > via < /em > negative regulation of FRAT1
Studies have shown that swertiamarin (STM) has multiple biological activities, but its anti-tumour effects and molecular mechanisms are still unclear. The present research aimed to validate the STM ’s impacts on the proliferation, migration, and invasion of hepatocellular carcinoma (HCC) cells, and to study its potential mechanism. Two HCC cell lines were treated with STM. Tumour growth was observed by the mouse tumour xenografts model. HCC cell lines stably expressing T-cell lymphomas 1 (FR AT1) were generated by lentivirusmediated overexpression. Cell viability, proliferation, migration, and invasion were observed usin...
Source: European Journal of Histochemistry - October 19, 2020 Category: Biomedical Science Authors: Shufeng Xiao, Haoren Tang, Yao Bai, Renchao Zou, Zongfang Ren, Xuesong Wu, Zhitian Shi, Song Lan, Wei Liu, Tiangen Wu, Cheng Zhang, Lin Wang Source Type: research
Different iron-handling in inflamed small and large cholangiocytes and in small and large-duct type intrahepatic cholangiocarcinoma
Cholangiocarcinoma (CCA) represents the second most common primary hepatic malignancy and originates from the neoplastic transformation of the biliary cells. The intrahepatic subtype includes two morpho-molecular forms: large-duct type intrahepatic CCA (iCCA) and small-duct type iCCA. Iron is fundamental for the cellular processes, contributing in tumor development and progression. The aim of this study was to evaluate iron uptake, storage, and efflux proteins in both lipopolysaccharide-inflamed small and large cholangiocytes as well as in different iCCA subtypes. Our results show that, despite an increase in interleukin-6...
Source: European Journal of Histochemistry - October 19, 2020 Category: Biomedical Science Authors: Romina Mancinelli, Antimo Cutone, Luigi Rosa, Maria Stefania Lepanto, Paolo Onori, Luigi Pannarale, Antonio Franchitto, Eugenio Gaudio, Piera Valenti Source Type: research
IncRNA MAPKAPK5-AS1 promotes proliferation and migration of thyroid cancer cell lines by targeting miR-519e-5p/YWHAH
The objective of our study was to explore the role and mechanism of MAPKAPK5-AS1 in thyroid cancer cells and provide a potential target for its biological diagnosis and treatment. We transfected sh-MAPKAPK5-AS1 and sh-NC into BCPAP a nd TPC-1 cells for loss-of-function assays. Results of RT-qPCR analysis demonstrated that MAPKAPK5-AS1 was more highly expressed in thyroid cancer cells compared to normal cells. Functional assays demonstrated that interfering with the expression of MAPKAPK5-AS1 notably repressed proliferation and i nvasion and accelerated apoptosis of BCPAP and TPC-1 cells. Mechanistically, we found that miR-...
Source: European Journal of Histochemistry - October 19, 2020 Category: Biomedical Science Authors: Yan Zhou, Shanshan Liu, Yan Luo, Meiying Zhang, Xueling Jiang, Yan Xiong Source Type: research
In memoriam of Prof. Antonio Lauria
(Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - September 28, 2020 Category: Biomedical Science Authors: Editor in Chief Source Type: research
Stem Cells and Therapy: Emerging Approaches
The ten chapters of this volume exploit the potentials of stem cells biology in translational medicine in a very clear way also thanks to nice color illustrations together with tricks and suggestions useful to get reproducible results... (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - September 11, 2020 Category: Biomedical Science Authors: Manuela Monti Source Type: research
An improved method for preparation of mouse retinal cryosections
Immunohistochemistry using mouse retinal cryosections is a routine assay used in vision research. However, retinal tissues are fragile, and it is difficult to obtain an ideal retinal cryosection. Here, we developed a modified method for preparing retinal cryosection. Super Glue was applied on the surface of the sclera before the cornea and the lens are removed from either the unfixed or PFA-fixed mouse eyeballs. The new methods largely prevented retinal detachment in mouse retinal cryosections. Immunostaining of retinal cryosections derived from PFA-fixed mouse eyes using rod and cone markers yielded high-quality...
Source: European Journal of Histochemistry - September 3, 2020 Category: Biomedical Science Authors: Ling Li, Yongqiong Lin, Fang Yang, Tongdan Zou, Jialiang Yang, Houbin Zhang Source Type: research
