Seasonal expressions of VEGF and its receptors VEGFR1 and VEGFR2 in the prostate of the wild ground squirrels ( < em > Spermophilus dauricus < /em > )
In this study, we investigated the localizations and expressions of vascular endothelial growth factor (VEGF) and its receptors including VEGF-receptor1 (VEFGR1) and VEGF-receptor2 (VEGFR2) in the prostate of the wild ground squirrels during the breeding and the non-breeding seasons. The values of total prostate weight and volume in the breeding season were higher than those in the non-breeding season. Histological observations showed that the exocrine lumens of the prostate expanded in the breeding season and contracted in the non-breeding season. The mRNA expression levels of VEGF and VEGFR2 in the prostate were higher i...
Source: European Journal of Histochemistry - March 24, 2021 Category: Biomedical Science Authors: Yuchen Yao, Wenqian Xie, Di Chen, Yingying Han, Zhengrong Yuan, Haolin Zhang, Qiang Weng Source Type: research
Immunohistochemistry as a paramount tool in research of normal urothelium, bladder cancer and bladder pain syndrome
The urothelium, an epithelium of the urinary bladder, primarily functions as blood-urine permeability barrier. The urothelium has a very slow turn-over under normal conditions but is capable of extremely fast response to injury. During regeneration urothelium either restores normal function or undergoes altered differentiation pathways, the latter being the cause of several bladder diseases. In this review, we describe the structure of the apical plasma membrane that enables barrier function, the role of urothelium specific proteins uroplakins and the machinery for polarized membrane transports in terminally differentiated...
Source: European Journal of Histochemistry - March 24, 2021 Category: Biomedical Science Authors: Da ša Zupančič, Rok Romih Source Type: research
Ultrastructural immunocytochemistry shows impairment of RNA pathways in skeletal muscle nuclei of old mice: A link to sarcopenia?
In this study, the distribution and density of key molecular factors involved in RNA pathways namely, nuclear actin (a motor protein and regulator of RNA transcription), 5-methyl cytosine (an epigenetic regulator of gene transcription), and ribonuclease A (an RNA degrading enzyme) were compared in different nuclear compartments of late adult and old mice myonuclei by means of ultrastructural immunocytochemistry. In all nuclear compartments, an age-related decrease of nuclear actin suggested altered chromatin structuring and impaired nucleus-to-cytoplasm transport of both mRNA and ribosomal subunits, while a decrease o...
Source: European Journal of Histochemistry - March 24, 2021 Category: Biomedical Science Authors: Maria Assunta Lacavalla, Barbara Cisterna, Carlo Zancanaro, Manuela Malatesta Source Type: research
PD-L1 expression with QR1 and E1L3N antibodies according to histological ovarian cancer subtype: A series of 232 cases
Therapeutic strategies for epithelial ovarian cancers are evolving with the advent of immunotherapy, such as PD-L1 inhibitors, with encouraging results. However, little data are available on PDL-1 expression in ovarian cancers. Thus, we set out to determine the PD-L1 expression according to histological subtype. We evaluated the expression of two PD-L1 clones – QR1 and E1L3N – with two scores, one based on the percentage of labeled tumor cells (tumor proportion score, TPS) and the other on labeled immune cells (combined proportion score, CPS) in a consecutive retrospective series of 232 ovarian cancers. PD-L1 expressio...
Source: European Journal of Histochemistry - March 10, 2021 Category: Biomedical Science Authors: Caroline Eymerit-Morin, Anna Ilenko, Thomas Gaillard, Justine Varinot, Eva Comp érat, Sofiane Bendifallah, Emile Darai Source Type: research
Evaluation of expression of Toll-Like Receptors 7 and 9, proliferation, and cytoskeletal biomarkers in plaque and guttate psoriasis: A pilot morphological study
This pilot study was aimed at comparing TLR7/TLR9 expression, cytoskeletal arrangement, and cell proliferation by indirect immunofluorescence in parallel lesional and non lesional skin samples of guttate psoriasis (PG) and psoriasis vulgaris (PV) in five male patients for each group (n=10). TLR7 expression was detected throughout all the epidermal compartment in PV samples, while in PG skin was restricted to the granular layer. TLR9 was present in the granular layer of non lesional skin and in the suprabasal layers of PV/PG lesional skin. Cell proliferation was localized in all the epidermal layers in lesional PG and PV, c...
Source: European Journal of Histochemistry - March 5, 2021 Category: Biomedical Science Authors: Francesca Prignano, Giulia Lombardo, Serena Indino, Federica Ricceri, Laura Cornaghi, Elena B. Donetti Source Type: research
Aberrant expression of < em > CCDC69 < /em > in breast cancer and its clinicopathologic significance
Coiled-coil domain-containing protein 69 (CCDC69) is a novel gene and limited knowledge in known in breast cancer. In the present study, we aimed to explore the relationship betweenCCDC69 and breast cancer, demonstrate the clinicopathological significance and prognostic role ofCCDC69 in breast cancer, and analyze the possible mechanism ofCCDC69 affecting the prognosis of breast cancer. First, from GEO database, TIMER, GEPIA, and OncoLnc, we selectCCDC69 as the potential gene which closely involved in breast cancer progression. Next, by real-time PCR detection, the expression ofCCDC69 in breast cancer tissue was notably low...
Source: European Journal of Histochemistry - February 22, 2021 Category: Biomedical Science Authors: Jinjiao Li, Panshi Zhang, Yun Xia Source Type: research
Study on < em > CCDC69 < /em > interfering with the prognosis of patients with breast cancer through PPAR signal pathway
Coiled-coil domain-containing protein 69 (CCDC69) is a novel gene and limited knowledge in known in breast cancer. In the present study, we aimed to explore the relationship betweenCCDC69 and breast cancer, demonstrate the clinicopathological significance and prognostic role ofCCDC69 in breast cancer, and analyze the possible mechanism ofCCDC69 affecting the prognosis of breast cancer. First, from GEO database, TIMER, GEPIA, and OncoLnc, we selectCCDC69 as the potential gene which closely involved in breast cancer progression. Next, by real-time PCR detection, the expression ofCCDC69 in breast cancer tissue was notably low...
Source: European Journal of Histochemistry - February 22, 2021 Category: Biomedical Science Authors: Jinjiao Li, Panshi Zheng, Yun Xia Source Type: research
miR-26b regulates cell proliferation and apoptosis of CD117+CD44+ ovarian cancer stem cells by targeting PTEN
Ovarian cancer (OC) is the one of the most common cancer in women globally. However, it still represents the most dangerous gynecologic malignancy even with the advances in detection and therapeutics. Thus, there is an urgent need in finding more effective therapeutic options for OC patients including cancer stem cells (CSC). MicroRNAs (miRNAs) are small, endogenous, and non-coding RNAs that play critical roles in the progression of various types of tumor. Our aim of this study was to find the regulatory function of microRNA-26 (miRNA-26b) on the cell proliferation and apoptosis of ovarian CSCs. Our studies show that miR-2...
Source: European Journal of Histochemistry - February 4, 2021 Category: Biomedical Science Authors: Zubiao Gao, Xiaofeng Ye, Anne Bordeaux, Stanka Hettich, Siyao Lin, Fang Han, Yan Jia Source Type: research
Tripartite motif-containing protein 6 facilitates growth and migration of breast cancer through degradation of STUB1
In conclusion, TRIM6 contributed to breast cancer progression through ubiquitination-dependent proteasomal degradation of STUB1 and provocation of YAP1 pathway, providing potential therapeutic target for breast cancer. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - January 20, 2021 Category: Biomedical Science Authors: Chuanchao Wei, Jiayue Wu, Weiyan Liu, Jingfeng Lu, Hongchang Li, Benjun Hai Source Type: research
Immunopositivity for Siglec-15 in gastric cancer and its association with clinical and pathological parameters
The sialic acid-binding immunoglobulin-type lectin Siglec-15 is a promising target to cancer immunotherapy in several tumor types. The present study aimed to investigate Siglec-15 expression in gastric cancer (GC) patient tissue and to evaluate its clinical value. Siglec-15 expression was evaluated by immunohistochemistry with 71 patients. Siglec-15 staining was observed in tumor cells of 53 (74.64%) patients, with significant association with histologic classification and angiolymphatic invasion (p<0.05). Immunohistochemistry analysis also detected Siglec-15 in tumor-associated stroma cells (macrophages/myeloid cells)....
Source: European Journal of Histochemistry - January 20, 2021 Category: Biomedical Science Authors: Michael Williams Leal Quirino, Michelly Cristiny Pereira, Maria de F átima Deodato de Souza, Ivan da Rocha Pitta, Antônio Felix da Silva Filho, Mario S. de Souza Albuquerque, Amanda Pinheiro de Barros Albuquerque, Mário Rino Martins, Maira Galdino da R Source Type: research
New bitongling (NBTL) ameliorates rheumatoid arthritis in rats through inhibiting JAK2/STAT3 signaling pathway
Rheumatoid arthritis (RA) is featured by a variety of physical symptoms and fibroblast-like synoviocytes (FLSs) abnormal proliferation. Increasing evidence has demonstrated that traditional Chinese medicine exerts an important role in RA treatment. New bitongling (NBTL) as one of the traditional Chinese medicine has been reported to be involved in the progression of RA, but the exact mechanism is unclear. In our study, we intended to investigate the effect of NBTL on RA to identify the mechanisms related to JAK2/STAT3 signaling pathway. Extracts ofTripterygium wilfordii (TW), a traditional Chinese herbal medicine, have bee...
Source: European Journal of Histochemistry - January 20, 2021 Category: Biomedical Science Authors: Xiang Li, Yu Xie, An Kang, Yue Wang Source Type: research
Effects of rutin on osteoblast MC3T3-E1 differentiation, ALP activity and Runx2 protein expression
As a flavonoid, rutin has been found to have a wide range of biological functions, such as resisting inflammation and oxidation, and preventing cerebral hemorrhage and hypertension. It has been found to play an important role in osteoporosis and other orthopedic diseases in recent years. MC3T3-E1 cells were randomly divided into a control group, a rutin-1 group (0.01 mmol/L), a rutin-2 group (0.05 mmol/L) and a rutin-3 group (0.1 mmol/L). Osteogenic differentiation of cells was induced by osteogenic induction fluid. The control group was treated with the maximum dose of drug solvent. 2~3 days later, the solvent was replace...
Source: European Journal of Histochemistry - January 20, 2021 Category: Biomedical Science Authors: Xin-Wei Liu, Bin Ma, Ying Zi, Liang-Bi Xiang, Tian-Yu Han Source Type: research
Expressions of ZNF436, β-catenin, EGFR, and CMTM5 in breast cancer and their clinical significances
As the leading malignancy among women, breast cancer is a serious threat to the life and health of women. In this context, it is of particular importance that a proper therapeutic target be identified for breast cancer treatment. We collected the pathological tissues of 80 patients, with the view to discovering appropriate molecular targets for the treatment of breast cancer, this paper analyzes the expressions of ZNF436, β-catenin, EGFR and CMTM5 in breast cancer tissues, as well as their correlations with breast cancer in combination with the clinicopathologic characteristics of studied patients. Immunohistochemistry (I...
Source: European Journal of Histochemistry - January 20, 2021 Category: Biomedical Science Authors: Zhi Chen, Na Cui, Ji-sen Zhao, Jian-fei Wu, Fang Ma, Cong Li, Xian-yi Liu Source Type: research
PDTC ameliorates neuropathic pain by inhibiting microglial activation < em > via < /em > blockage of the TNF α-CX3CR1 pathway
In conclusion, intrathecal infusion of PDTC could attenuate the pain-related behaviors induced by sciatic nerve CCI through suppressing the spinal microglia activation and TNF-a up-regulation in rats. The NF- κB activation might be responsible for TNF-a-induced CX3CR1 up-regulation in microglia. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - January 20, 2021 Category: Biomedical Science Authors: Xilei Li, Zhi Ye, Qulian Guo, E Wang, Yundan Pan Source Type: research
Twenty years of histochemistry in the third millennium, browsing the scientific literature
Over the last twenty years, about 240,000 articles where histochemical techniques were used have been published in indexed journals, and their yearly number has progressively increased. The histochemical approach was selected by researchers with very different scientific interests, as the journals in which these articles were published fall within 140 subject categories. The relative proportion of articles in some of these journal categories did change over the years, and browsing the table of contents of the European Journal of Histochemistry, as an example of a strictly histochemical journal, it appeared t...
Source: European Journal of Histochemistry - December 29, 2020 Category: Biomedical Science Authors: Carlo Pellicciari Source Type: research
