Deleted in Liver Cancer 2 (DLC2) protein expression in hepatocellular carcinoma
We examined two types of hepatocellular carcinoma- typical and fibrolamellar one. Our analysis revealed that DLC2 protein is not diminished in cancer tissue when compared to non-cancerous liver specimens. What is more, we observed DLC2 to be more abundantly expressed in cancer tissue, particularly in tumors with the inflammation background. In addition, we found thatDLC2 gene status was diploid in virtually all tumor samples examined. Our results indicate that DLC2 is not diminished in hepatocellular carcinoma cells. It appears that members of the DLC family, although structurally highly related, may function differently i...
Source: European Journal of Histochemistry - February 18, 2019 Category: Biomedical Science Source Type: research
Morphological and ultrastructural analysis of normal, injured and osteoarthritic human knee menisci
The human meniscus plays a crucial role for transmission and distribution of load across the knee, as well as shock absorption, joint stability, lubrication, and congruity. The aim of this study was to compare the complex geometry, and unique ultrastructure and tissue composition of the meniscus in healthy (control) and pathological conditions to provide understanding of structural changes that could be helpful in the future design of targetted therapies and improvement of treatment indications. We analyzed meniscus samples collected from 3 healthy multi-organ donors (median age, 66 years), 5 patients with traumatic menisc...
Source: European Journal of Histochemistry - February 11, 2019 Category: Biomedical Science Source Type: research
Antigen retrieval pre-treatment causes a different expression pattern of Cav3.2 in rat and mouse spinal dorsal horn
Cav3 channels consist of three isoforms, Cav3.1 ( α1G), Cav3.2 ( α1H), and Cav3.3 ( α1I), which produce low-threshold spikes that trigger burst firings in nociceptive neurons of the spinal dorsal horn (SDH) and dorsal root ganglion (DRG). Although Cav3.2 plays a crucial role in pathological pain, its distribution in SDH still remains controversial. One study showed that Cav3.2 is ubiquitously expressed in neurons, but another study implied that Cav3.2 is expressed restricted to astrocytes. To unravel these discrepancies, we used methods of immunohistochemistry either with or without antigen retrieval (AR) pre-treatment ...
Source: European Journal of Histochemistry - January 23, 2019 Category: Biomedical Science Source Type: research
Relationship between lipid droplets size and integrated optical density
Lipid accumulation is largely investigated due to its role in many human diseases. The attention is mainly focused on the lipid droplets (LDs), spherical cytoplasmic organelles, which are devoted to the storage of the lipids. The amount of lipid content is often evaluated by measuring LDs size and/or the integrated optical density (IOD) in cultured cells. Both evaluations are directly associated to the lipid content and therefore they are correlated to each other, but a lack of theoretical relationship between size and IOD was observed in literature. Here we investigated the size-IOD relationship of LDs observed in microsc...
Source: European Journal of Histochemistry - January 17, 2019 Category: Biomedical Science Source Type: research
VEGF, VEGF165b and EG-VEGF expression is specifically related with hormone profile in pituitary adenomas
Vascular endothelial growth factor (VEGF), its inhibitory splice variant, VEGF165band Endocrine Gland derived VEGF (EG-VEGF) have a controversial role in pituitary gland. We aim to study VEGF, VEGF165b and EG-VEGF expression in pituitary adenomas. A significant correlation was found between growth hormone (GH) and VEGF secretion (P=0.024). For prolactinomas, VEGF and prolactin expression, had a P-value of 0.02 for Kendall coefficient and a P-value of 0.043 for the Spearman coefficient. VEGF-mRNA amplification was detected in both tumor cells and folliculostellate cells. VEGF165b was positive in 16.66% of pituitary adenomas...
Source: European Journal of Histochemistry - January 17, 2019 Category: Biomedical Science Source Type: research
Immunohistochemical distribution of insulin-, glucagon- and somatostatin-containing cells in the pancreas of Lake Van fish (Alburnus tarichi G üldenstädt, 1814) (Cyprinidae)
In this study, the regional distribution, volume density, and relative frequency of some pancreatic endocrine cells in Lake Van fish were investigated via immunohistochemistry using specific mammalian antibodies. The pancreatic tissue was observed to be surrounded by adipose tis sue, which was adjacent to the gall bladder or extrahepatic bile duct, or dispersed in the adipose tissue ranked among coils of post-esophageal swelling and intestine. The pancreatic endocrine cells were examined, including the islets, exocrine pancreas, and pancreatic ducts. According to the modifi ed aldehyde fuchsin staining and immunohistochemi...
Source: European Journal of Histochemistry - January 17, 2019 Category: Biomedical Science Source Type: research
Seasonal expressions of prolactin, prolactin receptor and STAT5 in the scented glands of the male muskrats (Ondatra zibethicus)
Prolactin (PRL) production in mammals has been demonstrated in extrapituitary gland, which can activate autocrine/paracrine signaling pathways to regulate physiological activity. In the current study, we characterized the gene expression profiles of PRL, prolactin receptor (PRLR) and signal transducers and activators of transcription 5 (STAT5) in the scented glandular tissues of the muskrats, to further elucidate the relationship between PRL and the scented glandular functions of the muskrats. The weight and volume of the scented glands in the breeding season were significantly higher than those of the non-breeding season....
Source: European Journal of Histochemistry - January 17, 2019 Category: Biomedical Science Source Type: research
Sertoli Cells - Methods and Protocols
The mammalian somatic cell providing the physiological milieu to germ cells growth and differentiation is the Sertoli cell, named after the Italian physiologist Enrico Sertoli. Sertoli described for the first time this functionally multitasking cell in 1865 (when he was 23 years-old!) in human testicular tissues while ending up his medicine studies at the University of Pavia... (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - January 17, 2019 Category: Biomedical Science Source Type: research
Cell Migration - Methods and Protocols
As the title suggests, this book presents several techniques to study cells migration in vivo, in vitro, ex vivo and with different model systems to dissect many of the biochemical and biophysical properties (at both molecular and cellular levels) involved in the dynamics of migration and cell-to-cell communication... (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - January 17, 2019 Category: Biomedical Science Source Type: research
Epithelial expression of the hormone leptin by bovine skin
Leptin (Lep) stimulates keratinocytes to proliferate, intervenes in the wound healing and participates to hair follicle morphogenesis and cycle. While it is secreted by skin structures including epidermis and hair follicles, intradermal adipose tissue also seems to have a role in Lep secretion and accordingly in the control of hair follicle growth in mice and humans. Lep was investigated in the skin of humans and laboratory animals but there are not data regarding bovine species. The aim of this work was to study the expression of Lep and its receptor (LepR) in the skin of bovine and, at the same time, to investigate the p...
Source: European Journal of Histochemistry - January 17, 2019 Category: Biomedical Science Source Type: research
Histochemistry as a versatile research toolkit in biological research, not only an applied discipline in pathology
The impressive progress of histochemistry over the last 50 years has led to setting up specific and sensitive techniques to describe dynamic events, through the detection of specific molecules in the very place where they exist in live cells. The scientific field where histochemistry has most largely been applied is histopathology, with the aim to identify disease-specific molecular markers or to elucidate the etiopathological mechanisms. Numerous authors did however apply histochemistry to a variety of other research fields; their interests range from the microanatomy of animal and plant organisms to the cellular mechanis...
Source: European Journal of Histochemistry - December 21, 2018 Category: Biomedical Science Source Type: research
Two histological methods for recognition and study of cortical microinfarcts in thick sections
Cortical microinfarcts are the most widespread form of brain infarction but frequently remain undetected by standard neuroimaging protocols. Moreover, microinfarcts are only partially detectable in hematoxylin-eosin-stained (H and E) 4-10 µm paraffin sections at routine neuropathological examination. In this short report, we provide two staining protocols for visualizing cortical microinfarcts in 100-300 µm sections. For low-power microscopy, the first protocol combines aldehyde fuchsine staining for detection of lipofuscin granule s and macrophages with Darrow red counterstaining for Nissl material. The second protocol ...
Source: European Journal of Histochemistry - December 20, 2018 Category: Biomedical Science Source Type: research
Evolutionary intraembryonic origin of vertebrate hematopoietic stem cells in the elasmobranch spleen
The electric ray (Torpedo Marmorata Risso) provides an animal model for the detection of early intraembryonic hemopoietic stem cells in sea vertebrates. The spleen of this bone-marrowless vertebrate appears to be the major site of hemopoietic stem cell differentiation during development and in adulthood. Splenic development in this species was investigated and hemopoietic stem cells were detected in this organ by immunocytochemistry utilizing CD34 and CD38 antibodies. At stage I (2-cm-long embryos with external gills), the spleen contains only mesenchymal cells. At stage II (3-4 cm-long embryos with a discoidal shape and i...
Source: European Journal of Histochemistry - December 20, 2018 Category: Biomedical Science Source Type: research
Epidermal growth factor-like domain multiple 7 (EGFL7): Expression and possible effect on biliary epithelium growth in cholangiocarcinoma
In conclusion the expression of EGFL7 might be useful in the classification of CCA subtypes. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - November 30, 2018 Category: Biomedical Science Source Type: research
Dynamic of lipid droplets and gene expression in response to β-aminoisobutyric acid treatment on 3T3-L1 cells
This study analyzes the effect of different doses of BAIBA and at different days of development on 3T3-L1 cells by evaluating morphological changes in LDs and the expression of browning gene markers. Results suggested that the highest concentration of BAIBA after 4 days of differentiation produced the most significant effects. The number of LDs per cell increased in comparison to control cells, whereas the surface area significantly decreased. Brown adipocyte markers were up-regulated, but the effect of treatment was lost at 10 days of differentiation. The thermogenic program induced by BAIBA may reflect a rapid adaptation...
Source: European Journal of Histochemistry - November 28, 2018 Category: Biomedical Science Source Type: research
