Effect of TNBS-induced colitis on enteric neuronal subpopulations in adult zebrafish
This study focuses on the effect of 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis on changes in enteric neuronal subpopulations in adult zebrafish. These changes are suggested to be related to the altered neuro-immune interactions and GI motility, and in IBD pathogenesis. New insights into neuroplasticity will be instrumental in finding appropriate therapeutic treatments. TNBS was intraluminally administered in the distal intestine (DI) of anesthetized adult zebrafish. A histological time course of the intestinal inflammatory response was created to establish optimal TNBS concentration and acute inflammation ...
Source: European Journal of Histochemistry - August 28, 2020 Category: Biomedical Science Authors: Leen Uyttebroek, Casper Pype, Guy Hubens, Jean-Pierre Timmermans, Luc van Nassauw Source Type: research
Effect of carboplatin injection on Bcl-2 protein expression and apoptosis induction in Raji cells
To investigate the effects of carboplatin (CBP) injection on apoptosis induction in the human lymphoma cell line Raji and to explore the underlying mechanism, Raji cells were randomly divided into two treatment groups. Cells in the experimental groups were treated with 15 μM CBP injection, those in the control groups were treated with solvent, and both groups were treated for 24, 48 and 72 h. Cells from each group were collected for subsequent assays. For each group, the relative expression of B-cell lymphoma-2 (Bcl-2) was determined by Western blot (WB), the expres sion pattern of Bcl-2 was observed by immunocytochemistr...
Source: European Journal of Histochemistry - July 1, 2020 Category: Biomedical Science Authors: Peng Lin, Boliang Zhou, Haiying Yao, Ya-ping Guo Source Type: research
Imaging techniques in nanomedical research
About twenty years ago, nanotechnology began to be applied to biomedical issues giving rise to the research field called nanomedicine. Thus, the study of the interactions between nanomaterials and the biological environment became of primary importance in order to design safe and effective nanoconstructs suitable for diagnostic and/or therapeutic purposes. Consequently, imaging techniques have increasingly been used in the production, characterisation and preclinical/clinical application of nanomedical tools. This work aims at making an overview of the microscopy and imaging techniquesin vivo andin vitro in their applicati...
Source: European Journal of Histochemistry - July 1, 2020 Category: Biomedical Science Authors: Laura Calderan, Manuela Malatesta Source Type: research
Dark-field microscopy enhance visibility of CD31 endothelial staining
A simple dark field microscopy technique was used for visualization of blood vessels in normal human renal tissues and carcinoma. Phase contrast condenser ring apt for high power objectives was combined with a 10x objective in order to create a dark field illumination of the specimens examined. The endothelial lining of the vessels had been stained by using CD31 monoclonal antibodies combined with conventional peroxidase immunohistochemistry. The final DAB addition used for this technique induced an intense light scatter in the dark field microscope. This scattered light originating from the endothelial lining made the wal...
Source: European Journal of Histochemistry - July 1, 2020 Category: Biomedical Science Authors: Eva Jennische, Stefan Lange, Ragnar Hultborn Source Type: research
The synovial surface of the articular cartilage
The articular cartilage has been the subject of a huge amount of research carried out with a wide array of different techniques. Most of the existing morphological and ultrastructural data on the this tissue, however, were obtained either by light microscopy or by transmission electron microscopy. Both techniques rely on thin sections and neither allows a direct, face-on visualization of the free cartilage surface (synovial surface), which is the only portion subject to frictional as well as compressive forces. In the present research, high resolution visualization by scanning electron microscopy and by atomic force micros...
Source: European Journal of Histochemistry - July 1, 2020 Category: Biomedical Science Authors: Petra Rita Basso, Elena Carava ' , Marina Protasoni, Marcella Reguzzoni, Mario Raspanti Source Type: research
Combined lectin- and immuno-histochemistry (CLIH) for applications in cell biology and cancer diagnosis: Analysis of human urothelial carcinomas
Lectin histochemistry (LHC) and immunohistochemistry (IHC), which demonstrate the composition and localisation of sugar residues and proteins in cell membranes, respectively, are generally used separately. Using these two methods, we previously demonstrated that malignant transformation of urothelial cells results in the alterations of protein glycosylation and reduced expression of urothelium-specific integral membrane proteins uroplakins (UPs). However, the correlation between these changes was not studied yet. To evaluate this correlation, we developed innovative method, which we named combined lectin- and immuno- histo...
Source: European Journal of Histochemistry - July 1, 2020 Category: Biomedical Science Authors: Da ša Zupančič, Mateja Erdani Kreft, Igor Sterle, Rok Romih Source Type: research
A morphological analysis of fresh and brine-cured olives attacked by < em > Bactrocera oleae < /em > using light microscopy and ESEM-EDS
The present study investigated the morphology of fresh and brine-cured table olives (TOs) as well as the changes that occur when drupes are attacked by the fruit flyBactrocera oleae. Morphological analyses were performed using light microscopy (LM) and environmental scanning electron microscopy coupled with energy dispersive spectroscopy (ESEM-EDS). The LM analysis was carried out with visible light to evaluate sections stained with either PAS or Azan mixtures as well as unstained sections observed at fluorescence microscopy. The results of the analyses showed that: i) Azan and PAS staining played a useful complementary ro...
Source: European Journal of Histochemistry - July 1, 2020 Category: Biomedical Science Authors: Barbara Lanza, Anna Panato, Laura Valentini, Pamela Rodegher, Federica Bortolotti, Michela Battistelli, Paolino Ninfali, Pietro Gobbi Source Type: research
β-catenin mediates the effect of GLP-1 receptor agonist on ameliorating hepatic steatosis induced by high fructose diet
The hypoglycemic drug GLP-1 receptor agonist can ameliorate hepatic steatosis but the mechanism is not clear. Intake of high fructose leads to non-alcoholic fatty liver disease by stimulating lipid synthesis, and β-catenin is the key molecule for realizing GLP-1 function in extrahepatic tissues; with the discovery of GLP-1 receptor in liver, we speculate that β-catenin might mediate GLP-1 receptor agonist on ameliorating hepatic steatosis induced by high fructose. Wistar rats were fed with high fructose di et for 8 weeks and then treated with GLP-1 receptor agonist exenatide for 4 weeks; the changes of lipid synthesis pa...
Source: European Journal of Histochemistry - July 1, 2020 Category: Biomedical Science Authors: Zhe Gao, Guang-Yao Song, Lu-Ping Ren, Hui-Juan Ma, Bo-Qing Ma, Shu-Chun Chen Source Type: research
Morphological and ultrastructural characteristics of the tongue of wild boar
In conclusion, we found new descriptions and shapes of the CTCs of the lingual papillae. In addition, we demonstrated the epithelium structural characteristics, the nuclear distribution between the epithelial layers, and the ultrastructural aspects of the dorsal epithelium of the tongue. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - May 5, 2020 Category: Biomedical Science Authors: Gabriela de Souza Reginato, Gabriela Klein Barbosa, Amanda Olivotti Ferreira, Bruno Gomes Vasconcelos, Rose Eli Grassi Rici, Ii-sei Watanabe, Adriano Polican Ciena Source Type: research
Defense and protection mechanisms in lung exposed to asbestiform fiber: the role of macrophage migration inhibitory factor and heme oxygenase-1
Fluoro-edenite (FE), an asbestiform fiber, is responsible for many respiratory pathologies: chronic obstructive diseases, pleural plaques, fibrosis, and malignant mesothelioma. Macrophage migration inhibitory factor (MIF) is one of the first cytokines produced in response to lung tissue damage. Heme oxygenase-1 (HO-1) is a protein with protective effects against oxidative stress. It is up regulated by several stimuli including pro-inflammatory cytokines and factors that promote oxidative stress. In this research, thein vivo model of sheep lungs naturally exposed to FE was studied in order to shed light on the pathophysiolo...
Source: European Journal of Histochemistry - April 16, 2020 Category: Biomedical Science Source Type: research
MMP-7 and MMP-9 are overexpressed in the synovial tissue from severe temporomandibular joint dysfunction
In conclusion, MMP-7 and MMP-9 were overexpressed in the synovial tissue of patients with ADDwoR. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - April 16, 2020 Category: Biomedical Science Source Type: research
Ozone at low concentrations does not affect motility and proliferation of cancer cells in vitro
Exposure to low ozone concentrations is used in medicine as an adjuvant/complementary treatment for a variety of diseases. The therapeutic potential of low ozone concentrations relies on their capability to increase the nuclear translocation of the Nuclear factor erythroid 2-related factor 2 (Nrf2), thus inducing the transcription of Antioxidant Response Elements (ARE)-driven genes and, through a cascade of events, a general cytoprotective response. However, based on the controversial role of Nrf2 in cancer initiation, progression and resistance to therapies, possible negative effects of ozone therapy may be hypothesised i...
Source: European Journal of Histochemistry - April 2, 2020 Category: Biomedical Science Source Type: research
Isolation of rat hepatocytes for pharmacological studies on metabotropic glutamate receptor (mGluR) subtype 5: a comparison between collagenase I versus collagenase IV
Isolated hepatocytes can be obtained from the liver by collagenase infusion, a procedure that could affect cell isolation as well as the integrity of membrane receptors. In this respect we compared metabotropic glutamate subtype 5 receptor (mGluR5) protein expression and activity in rat hepatocytes isolated by two collagenases, type I and type IV. Hepatocytes were isolated from male Wistar rats (200-250 g) using collagenase I or collagenase IV and after isolation, viability and morphology of rat hepatocytes were assessed measuring mGluR5 protein expression by Western blot analyses. mGluR5 activation was evaluated by inosit...
Source: European Journal of Histochemistry - March 26, 2020 Category: Biomedical Science Source Type: research
A new method for the direct tracking of < em > in vivo < /em > lignin nanocapsules in < em > Eragrostis tef < /em > (Poaceae) tissues
Environmental concerns have driven scientists to research new eco-friendly approaches for the preparation of nanosystems. For this purpose, novel bio-polymers have been selected. Among these, one of the most promising is lignin, which is biodegradable and biocompatible. Additionally, lignin is one of the main by-products of the paper industry and can be re-used in nanosystems building. Lignin-based nanosystems could be used in agriculture, to improve the uptake of bioactive compounds, thus avoiding soil pollution. However, the mechanism of penetration in the plant and the route of transportation within the internal plant t...
Source: European Journal of Histochemistry - March 26, 2020 Category: Biomedical Science Source Type: research
A new method for the direct tracking of < em > in vivo < /em > lignin nanocapsules in Eragrostis tef (Poaceae) tissues
Environmental concerns have driven scientists to research new eco-friendly approaches for the preparation of nanosystems. For this purpose, novel bio-polymers have been selected. Among these, one of the most promising is lignin, which is biodegradable and biocompatible. Additionally, lignin is one of the main by-products of the paper industry and can be re-used in nanosystems building. Lignin-based nanosystems could be used in agriculture, to improve the uptake of bioactive compounds, thus avoiding soil pollution. However, the mechanism of penetration in the plant and the route of transportation within the internal plant t...
Source: European Journal of Histochemistry - March 26, 2020 Category: Biomedical Science Source Type: research
