How to stain nucleic acids and proteins in Miller spreads
The spread technique proposed by Miller and Beatty in 1969 allowed for the first time the visualization at transmission electron microscopy of nucleic acids and chromatin in an isolated and distended conformation. The final step of staining the spread chromatin is of critical importance because it can strongly influence the interpretation of the results. We evaluated different staining techniques and the most part of them provided a good result. Specifically, well contrasted micrographs were obtained when staining with H3PW12O40 (PTA), as originally proposed by Miller and Beatty, and with two alternatives proposed here: ur...
Source: European Journal of Histochemistry - January 12, 2022 Category: Biomedical Science Authors: Lorena Zannino, Marco Biggiogera Source Type: research
High expression of both desmoplastic stroma and epithelial to mesenchymal transition markers associate with shorter survival in pancreatic ductal adenocarcinoma
We examined the expression levels of DS markers alpha smooth muscle actin ( α-SMA), fibronectin, and vimentin, and the EMT markers epithelial cell adhesion molecule (EPCAM), pan-cytokeratin, and vimentin, by immunohistochemistry using a tissue microarray of a retrospective cohort of 25 patients with PDAC. The results were examined for association with survival by clinical stage and by degree of tumor differentiation. High DS markers expression -α-SMA, fibronectin, and vimentin- was associated with decreased survival at intermediate and advanced clinical stages (p=0.006-0.03), as well as with both poorly and moderately di...
Source: European Journal of Histochemistry - January 12, 2022 Category: Biomedical Science Authors: Dami án Sánchez-Ramírez, Rafael Medrano-Guzmán, Fernando Candanedo-González, Jazmín De Anda-González, Luis Enrique García-Rios, Vadim Pérez-Koldenkova, Marcos Gutiérrez-de la Barrera, Sara Rodríguez-Enríquez, Marco Velasco-Velázquez, Silvia C Source Type: research
Effects of miR-939 and miR-376A on ulcerative colitis using a decoy strategy to inhibit NF- κB and NFAT expression
This study provides new ideas for the development of future clinical therapies for UC. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - January 12, 2022 Category: Biomedical Science Authors: Yongwei Lin, Zhipeng Zhou, Lang Xie, Yongsheng Huang, Zhenghua Qiu, Lili Ye, Chunhui Cui Source Type: research
A spectrofluorometric analysis to evaluate transcutaneous biodistribution of fluorescent nanoparticulate gel formulations
The investigation of the absorption of drug delivery systems, designed for the transport of therapeutic molecules inside the body, could be relatively simplified by the fluorophore association and tracking by means of bio-imaging techniques (i.e., opticalin vivo imaging or confocal and multiphoton microscopy). However, when a fluorescence signal comes out from the skin, its specific detection can be problematic. Skin high autofluorescence can hinder the observation of administered exogenous fluorophores conjugated to drug delivery systems, making it more challenging to detect their biodistribution. In the present study, we...
Source: European Journal of Histochemistry - January 12, 2022 Category: Biomedical Science Authors: Enrica Cappellozza, Federico Boschi, Maddalena Sguizzato, Elisabetta Esposito, Rita Cortesi, Manuela Malatesta, Laura Calderan Source Type: research
In memoriam of Alberto Calligaro: Master of Histology and Life
(Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - January 12, 2022 Category: Biomedical Science Authors: Editor in Chief Source Type: research
Evaluation of biocompatibility, osteointegration and biomechanical properties of the new Calcemex ® cement: An < em > in vivo < /em > study
The mixture of polymethylmethacrylate (PMMA) and β-tricalciumphospate (β-TCP) is the most widely used bone graft. Common features of bone cement are the biocompatibility, bioactivity, mechanical stability and ability to fuse with the host ' s bone tissue. However, there are still few studies that have evaluated these characteristicsin vivo. Our study aims to acquire these parameters, using an animal model with functional characteristics similar to those of humans. The analyzed cement is Calcemex®, evaluated both in compact and fluid formulation. The chosen animal models were 5 pigs, treated with femoral and tibial impla...
Source: European Journal of Histochemistry - January 12, 2022 Category: Biomedical Science Authors: Tommaso Maluta, Umberto Lavagnolo, Lydia Segalla, Nicholas Elena, Paolo Bernardi, Daniele Degl ' Innocenti, Andrea Sbarbati, Bruno Magnan Source Type: research
Ghrelin reduces cerebral ischemic injury in rats by reducing M1 microglia/macrophages
The purpose of this study was to investigate the effect of Ghrelin on the polarization of microglia/ macrophages after cerebral ischemia (CI) in rats. 60 wild-type SD rats were randomly divided into sham group, CI group, CI+Ghrelin group, 20 rats in each group. The modified Longa suture method was used to establish the middle cerebral artery occlusion (MCAO) model in rats. Before surgery, Ghrelin was injected subcutaneously (100 μg/kg, twice a day) for 4 consecutive weeks. After modeling, neurological function scores were performed with three behavioral experiments: mNSS score, Corner test, and Rotarod test, to evaluate t...
Source: European Journal of Histochemistry - January 12, 2022 Category: Biomedical Science Authors: Rong Tian, Gengsheng Mao Source Type: research
< em > miR-19a < /em > targeting < em > CLCA4 < /em > to regulate the proliferation, migration, and invasion of colorectal cancer cells
In conclusion, our results revealed thatmiR-19a overexpression decreases CLCA4 levels to promote CRC oncogenesis, suggesting thatmiR-19a inhibitors have potential applications for future therapeutic of CRC. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - January 12, 2022 Category: Biomedical Science Authors: Huiwen Li, Bo Huang Source Type: research
Sufentanil alleviates cerebral ischemia-reperfusion injury by inhibiting inflammation and protecting the blood-brain barrier in rats
Stroke is a brain system disease with a high fatality rate and disability rate. About 80% of strokes are ischemic strokes. Cerebral ischemia-reperfusion injury (CIRI) caused by ischemic stroke seriously affects the prognosis of stroke patients. The purpose of this study is to investigate the effect of sufentanil (SUF) on CIRI model rats. We used middle cerebral artery occlusion (MCAO) to make the CIRI model in rats and monitored region cerebral blood flow (rCBF) to ensure that blood flow was blocked and recanalized. We used ELISA and RT-PCR to detect the expression of inflammatory factors in rat serum and brain tissue. In ...
Source: European Journal of Histochemistry - January 12, 2022 Category: Biomedical Science Authors: Zhen Wang, Xiaoyan Du, Daoyang Yu, Yang Yang, Gaoen Ma, Xueli Jia, Lulu Cheng Source Type: research
MicroRNA-17-3p is upregulated in psoriasis and regulates keratinocyte hyperproliferation and pro-inflammatory cytokine secretion by targeting < em > CTR9 < /em >
Psoriasis is a chronic inflammatory skin disease. Although miRNAs are reported to be associated with the pathogenesis of psoriasis, the contribution of individual microRNAs toward psoriasis remains unclear. The miR-17-92 cluster regulates cell growth and immune functions that are associated with psoriasis. miR-17-3p is a member of miR-17-92 cluster; however, its role in dermatological diseases remains unclear. Our study aims at investigating the effects of miR-17-3p and its potential target gene on keratinocytes proliferation and secretion of pro-inflammatory cytokine and their involvement in psoriasis. Initially, we found...
Source: European Journal of Histochemistry - January 12, 2022 Category: Biomedical Science Authors: Qingwen Li, Jiao Zhang, Shougang Liu, Fangfei Zhang, Jiayi Zhuang, Yongfeng Chen Source Type: research
Histochemistry for nanomedicine: Novelty in tradition
During the last two centuries, histochemistry has provided significant advancements in many fields of life sciences. After a period of neglect due to the great development of biomolecular techniques, the histochemical approach has been reappraised and is now widely applied in the field of nanomedicine. In fact, the novel nanoconstructs intended for biomedical purposes must be visualized to test their interaction with tissue and cell components. To this aim, several long-established staining methods have been re-discovered and re-interpreted in an unconventional way for unequivocal identification of nanoparticulates at both...
Source: European Journal of Histochemistry - December 27, 2021 Category: Biomedical Science Authors: Manuela Malatesta Source Type: research
miR-1270 enhances the proliferation, migration, and invasion of osteosarcoma < em > via < /em > targeting cingulin
In this study, miR-1270 was overexpressed in OS tissues and cells. However, miR-1270 knockdown inhibited the proliferation, migration and invasion, and promoted the OS cells ’ apoptosis. Mechanistically, cingulin (CGN) was predicted and proved to be a target of miR-1270 and partially alleviated the effects of miR-1270 on the proliferation, migration and invasion ability of OS cells. Taken together, knockdown of miR-1270 may inhibit the development of OSvia targeting CGN. This finding may provide a novel therapeutic strategy for OS. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - December 7, 2021 Category: Biomedical Science Authors: Yang Liu, Weichun Guo, Shuo Fang, Bin He, Xiaohai Li, Li Fan Source Type: research
The lncRNA MEG3 promotes trophoblastic cell growth and invasiveness in preeclampsia by acting as a sponge for miR-21, which regulates BMPR2 levels
This study aimed to investigate the potential impact and regulatory mechanisms of bone morphogenetic protein receptor 2 (BMPR2) on the progression of PE. We obtained placental tissues from pregnant women with PE and normal pregnant women, and the results showed that BMPR2 was expressed at low levels in the tissue from PE women. Genetic knockdown of BMPR2 increased the proliferation and invasion of cultured trophoblast cells, whereas its overexpression reduced these characteristics. Bioinformatics analysis and luciferase reporter gene assays confirmed thatBMPR2 is a direct target of miR-21. Overexpression of a miR-21 inhibi...
Source: European Journal of Histochemistry - November 25, 2021 Category: Biomedical Science Authors: Huyi Liu, Xiangdao Cai , Jia Liu, Fengxiang Zhang , Andong He, Ruiman Li Source Type: research
LncRNA FGD5-AS1 functions as an oncogene to upregulate GTPBP4 expression by sponging miR-873-5p in hepatocellular carcinoma
The long non-coding FGD5-AS1 (LncFGD5-AS1) has been reported to be a novel carcinogenic gene and participant in regulating tumor progression by sponging microRNAs (miRNAs). However, the pattern of expression and the biological role of FGD5-AS1 in hepatocellular carcinoma (HCC) remains largely unknown. The expression level of FGD5-AS1 in tumor tissues and cell lines was measured by RT-qPCR. CCK-8, EdU, flow cytometry, wound healing, and transwell chamber assays were performed to investigate the role of FGD5-AS1 in cell proliferation, apoptosis, migration, and invasion in HCC.Dual luciferase reporter, and RNA pull-down assay...
Source: European Journal of Histochemistry - November 16, 2021 Category: Biomedical Science Authors: Nuobei Zhang, Hao Shen, Shenan Huang, Fenfen Wang, Huifang Liu, Fen Xie, Lei Jiang, Xin Chen Source Type: research
The possible alleviating effect of garlic supplement on the neural retina in a rat model of hypercholesterolemia: a histological and immunohistochemical study
The purpose of this work was to prove that oxidative stress is the main mechanism responsible for retinal neurodegenerative changes, subsequent apoptosis, and inflammatory cytokine release in rats fed with a high cholesterol diet (HCD) and determine the role of garlic in alleviating these changes. Forty rats were equally divided into four groups: control, garlic-treated (positive control), HCD, and HCD + garlic-treated (HCD + G). By the end of the experiment (24 weeks) blood samples were collected for assessment of serum lipid profile, oxidative stress parameters, and plasma levels of IL-6 and TNF- α. Both eyes of the rat...
Source: European Journal of Histochemistry - November 2, 2021 Category: Biomedical Science Authors: Eetmad A. Arafat, Eman M.I. Youssef, Hanaa A. Khalaf Source Type: research
