Swertianolin ameliorates immune dysfunction in sepsis < em > via < /em > blocking the immunosuppressive function of myeloid- derived suppressor cells
In this study, we studied the long-term proliferation trajectory of myeloid-derived suppressor cells (MDSCs) in murine sepsis model and investigated whether swertianolin could modulate the immunosuppressive function of MDSCs. A murine sepsis model was established by cecal ligation and perforation (CLP), according to the Minimum Quality Threshold in Pre-Clinical Sepsis Studies (MQTiPSS) guidelines. The bone marrow and spleen of the mice were collected at 24 h, 72 h, 7 and 15 d after sepsis induction. The proportions of monocytic-MDSCs (M-MDSCs; CD11b+LY6G-LY6Chi) and granulocytic-MDSCs (G-MDSC, CD11b+Ly6G+ Ly6Clow) were ana...
Source: European Journal of Histochemistry - July 2, 2021 Category: Biomedical Science Authors: Zongfang Ren, Haoren Tang, Linjun Wan, Xing Liu, Ning Tang, Lingling Wang, Zhenhui Guo Source Type: research
Efficacy of immunohistochemical staining in detecting < em > Helicobacter pylori < /em > in Saudi patients with minimal and atypical infection
This study aimed to evaluate the efficacy of immunohistochemical (IHC) staining in detectingH. pyloriin gastric biopsies from patients with chronic gastritis and minimal or atypical infection. Gastric biopsies from 50 patients with chronic gastritis were subjected to routine haematoxylin and eosin (H&E), modified Giemsa, and IHC staining. The results of staining were compared with those of quantitative real-time PCR (qRT-PCR). The qRT-PCR analysis identified 32 (64%)H. pylori-positive cases, whereas IHC, H&E, and modified Giemsa staining identified 29 (58%), 27 (54%), and 21 (42%) positive cases. The sensitivity of...
Source: European Journal of Histochemistry - July 2, 2021 Category: Biomedical Science Authors: Mohammed Akeel, Ahmed Elhafey , Atef Shehata , Erwa Elmakki , Thanaa Aboshouk, Hussein Ageely , Mohammed Salih Mahfouz Source Type: research
Effects of the lncRNA ENST00000623984 on colon cancer and the biological characteristics of colon cancer cells
In this study, the expression levels of ENST000000623984 were first examined in tumor tissue and adjacent normal tissue from 40 patients with colorectal cancer and LoVo cells using quantitative real-time PCR. By siRNA transfection, ENST00000623984 expression was knocked down. Using flow cytometry, cell cycle progression and cell viability were examined in basal and knockdown LoVo cells. The CCK-8 assay was used to assess the cell proliferation rate, and the Transwell assay was used to determine the migration and invasion abilities. The ENST000000623984 expression level was increased in colorectal cancer. Knockdown of ENST0...
Source: European Journal of Histochemistry - July 2, 2021 Category: Biomedical Science Authors: Zhi-bao Liu, Jing-hua Zhang, Jing-hua Gao, Jian Shi Source Type: research
Characterization of PD-1/PD-L1 immune checkpoint expression in soft tissue sarcomas
This study aimed to retrospectively investigate the relationship between the expression of PD-1/PD-L1 and related antigens in STS, and their association with clinical characteristics. Immunostaining for CD4, CD8, PD-1, PD-L1, IL-2, and IFN- γ was performed using pathological specimens harvested at the time of biopsy from 10 patients with undifferentiated pleomorphic sarcoma (UPS), nine with myxofibrosarcoma (MFS), and three with malignant peripheral nerve sheath tumor (MPNST) who were treated at our hospital. Subsequently, the positiv e immunostaining cell rates were calculated. We also examined the correlation between ea...
Source: European Journal of Histochemistry - July 2, 2021 Category: Biomedical Science Authors: Kazuhiko Hashimoto, Shunji Nishimura, Tomohiko Ito, Masao Akagi Source Type: research
NOP14 regulates the growth, migration, and invasion of colorectal cancer cells by modulating the NRIP1/GSK-3 β/β-catenin signaling pathway
In this study, we used collected colorectal cancer tissues and cultured colorectal cancer cell lines (SW480, HT29, HCT116, DLD1, Lovo), and measured the mRNA and protein expression levels of NOP14 in colorectal cancer cells using qPCR and western blotting. GFP-NOP14 was constructed and siRNA fragments against NOP14 were synthesized to investigate the importance of NOP14 for the development of colorectal cells. Transwell migration assays were used to measure cell invasion and migration, CCK-8 kits were used to measure cell activity, and flow cytometry was applied to the observation of apoptosis. We found that both the mRNA ...
Source: European Journal of Histochemistry - July 2, 2021 Category: Biomedical Science Authors: Xuanjin Zhu, Weilu Jia, Yong Yan, Yong Huang, Bailin Wang Source Type: research
Perinatal Stem Cells - Biology, Manufacturing and Translational Medicine
This book focuses on a new category of stem cells derived from perinatal tissue, including amniotic fluid, fetal membranes, umbilical cord, and placental tissue.... (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - June 29, 2021 Category: Biomedical Science Authors: Manuela Monti Source Type: research
DNA damage and repair in differentiation of stem cells and cells of connective cell lineages: A trigger or a complication?
The review summarizes literature data on the role of DNA breaks and DNA repair in differentiation of pluripotent stem cells (PSC) and connective cell lineages. PSC, including embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC), are rapidly dividing cells with highly active DNA damage response (DDR) mechanisms to ensure the stability and integrity of the DNA. In PSCs, the most common DDR mechanism is error-free homologous recombination (HR) that is primarily active during S phase of the cell cycle, whereas in quiescent, slow-dividing or non-dividing tissue progenitors and terminally differentiated cells, er...
Source: European Journal of Histochemistry - May 3, 2021 Category: Biomedical Science Authors: Nikolajs Sjakste, Una Rieksti ņa Source Type: research
MicroRNA-29b-3p promotes 5-fluorouracil resistance < em > via < /em > suppressing TRAF5-mediated necroptosis in human colorectal cancer
In conclusion, it is deduced that miR-29b-3p/TRAF5 signaling axis plays critical role in drug resistance in chemotherapy for colorectal cancer patients by regulating necroptosis. The findings in this study provide us a new target for interfere therapy in colorectal cancer. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - March 24, 2021 Category: Biomedical Science Authors: Shuimei Wu, Yun Zhou, Ping Liu, Hui Zhang, Wanliang Wang, Yuan Fang, Xiang Shen Source Type: research
Potential effect of fish oil to preserve expression of cell cycle and tight junction regulating genes in colon after di-isononyl phthalate ingestion in albino Wistar rats
In conclusion, the current study provided an evidence that FO has a protective potential against DIP further examinations to be done to fully understand the molecular basis of this potential as a step for further clinical applications. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - March 24, 2021 Category: Biomedical Science Authors: Ahmed S. Ahmed, Hoda A. Ibrahim, Ehab M. Hantash Source Type: research
HPV infection upregulates the expression of ZNT-1 in condyloma acuminatum
This study provided new insights into the sign pathway to HPV infection. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - March 24, 2021 Category: Biomedical Science Authors: Li Niu, Xiaoying Chu, Yaofei Jiang, Wei Zeng Source Type: research
Immunoreactivities of AR, ER α, ERβ and aromatase in the nuptial pad of Chinese brown frog ( < em > Rana dybowskii < /em > ) during pre-hibernation and the breeding period
In this study, we investigated the immunolocalization and protein expression levels of AR, ER α, ERβ and aromatase in the nuptial pad ofR. dybowskii during pre-hibernation and the breeding period. Histologically, the annual development of the nuptial pad inR. dybowskii is manifested as the larger area of specialized mucous gland and the longer length of papillary epidermal projection during the breeding period. AR, ER α, ERβ and aromatase are present in the stratum granulosum, stratum spinosum, stratum basale and the secretory portion of specialized mucous glands during both periods. Western blotting results confirmed ...
Source: European Journal of Histochemistry - March 24, 2021 Category: Biomedical Science Authors: Zeqi Tang, Yuan Chen, Baolong Ren, Xi Wang, Haolin Zhang, Yingying Han, Zhengrong Yuan, Qiang Weng Source Type: research
Astragalus Ⅳ ameliorates the dry eye injury in rabbit model < em > via < /em > MUC1-ErbB1 pathway
The therapeutic effects and potential mechanisms of astragaloside IV on a rabbits dry eye model induced by benzalkonium chloride (BAC) was examined. In our study, a BAC-induced dry eye rabbit model was treated with eye drops containing astragaloside IV (5, 10 μM) or solvent four times a day. The clinical evaluations, such as tear break-up time (BUT) and Schirmer tear test (STT), were performed on days 0, 7, 14, 21, and 28. On day 28, the cornea and bulbar conjunctiva tissues (left eye and right eye) were collected with histology, and immunofluorescent s taining conducted. The levels of MUC1 and ErbB1in the corneas were de...
Source: European Journal of Histochemistry - March 24, 2021 Category: Biomedical Science Authors: Li Chu, Suhong Ma, Zhiwei Chen, Wenfu Cao Source Type: research
Comparison of CGRP distributions in the maxillary sinus and trigeminal ganglion between elderly dentulous and edentulous humans
Thickening of the Schneiderian membrane (SM, mucosa of the maxillary sinus) appears in the paranasal sinus. Information on SM thickening is available for patients receiving sinus lift treatments, which is a risk factor for SM excretory dysfunction. However, more information is needed on the structure of the SM and the relationship between the maxilla sinus and palatine with the alveolar bone and the SM for dental implant treatment in the human maxilla. One hundred twenty-six sides of the maxilla from 71 cadavers were subjected to cone-beam CT (CBCT) analysis and macroscopic and immunohistochemical observations in this stud...
Source: European Journal of Histochemistry - March 24, 2021 Category: Biomedical Science Authors: Hiroyuki Matsuda, Iwao Sato, Rieko Asaumi, Takuya Omotehara, Shinichi Kawata, Kenta Nagahori, Zhong-Lian Li, Masahiro Itoh Source Type: research
Overexpression of < em > MYBL2 < /em > predicts poor prognosis and promotes oncogenesis in endometrial carcinoma
Endometrial cancer (EC) is the most common gynecologic malignancy and still remains clinically challenging. We aimed to explore the potential biomarkers of EC and provide a theoretical basis for early screening and targeted therapy. The available transcriptome data from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) were analyzed to identify differentially expressed genes. Immunohistochemistry was performed to detect gene expression. We analyzed the associations ofMYBL2 with clinicopathological features and survival time and the biological effect ofMYBL2 on the proliferation of EC cells. The effect ofMYBL...
Source: European Journal of Histochemistry - March 24, 2021 Category: Biomedical Science Authors: Lulu Le, Ji Luo, Haifang Wu, Ling Chen, Xiaoli Tang, Fen Fu Source Type: research
The clinical significance of < em > UBE2C < /em > gene in progression of renal cell carcinoma
Renal cell carcinoma (RCC), with high morbidity and mortality, is one of the top ten serious cancers. Due to limited therapies and little knowledge about the mechanism underlying RCC, overall survival of RCC patients is poor. UBE2C is a member of ubiquitin modification system and promotes carcinogenesis in cancer, but its role in RCC is unknown. Based on the TCGA (The Cancer Genome Atlas) data, UBE2C was over-expressed in a total of 525 RCC tissues and displayed higher expression in advanced tissues (stage IVvs stage I, p<0.05). RT-qPCR and IHC analysis confirmed over-expression of UBE2C in 90 of clinical RCC tissues. F...
Source: European Journal of Histochemistry - March 24, 2021 Category: Biomedical Science Authors: Zhiping Chen, Lanfeng Wang Source Type: research
