A new method for the direct tracking of in vivo lignin nanocapsules in Eragrostis tef (Poaceae) tissues
Environmental concerns have driven scientists to research new eco-friendly approaches for the preparation of nanosystems. For this purpose, novel bio-polymers have been selected. Among these, one of the most promising is lignin, which is biodegradable and biocompatible. Additionally, lignin is one of the main by-products of the paper industry and can be re-used in nanosystems building. Lignin-based nanosystems could be used in agriculture, to improve the uptake of bioactive compounds, thus avoiding soil pollution. However, the mechanism of penetration in the plant and the route of transportation within the internal plant t...
Source: European Journal of Histochemistry - March 26, 2020 Category: Biomedical Science Source Type: research
Presence of N-acetylneuraminic acid in the lung during postnatal development
Sialic acids, particularly N-acetylneuraminic acid (Neu5Ac), are present as terminal components of rich and complex oligosaccharide chains, which are termed glycans, and are exhibited on the cell surfaces, especially on epithelial cells. Crucial in the ‘social behavior’ of the cell, sialic acids play vital roles in many physiological and pathological phenomena. The aim of the present study was to separate, identify, and quantify Neu5Ac in purified lung membranes from 4-, 14-, and 21-day-old animals, followed by the statistical analysis of thes e results with our previously reported data (0-day-old and adult results). C...
Source: European Journal of Histochemistry - March 26, 2020 Category: Biomedical Science Authors: Maria de F átima Martins, Marco S. Freitas, Ana Honório-Ferreira, Carlos Alberto Gonçalves Source Type: research
The substance P and neurokinin-1 receptor system in human thyroid cancer: an immunohistochemical study
This study aims to examine by immunohistochemistry, the localization of SP and the NK-1R in human TC samples (papillary, follicular, medullary, anaplastic), in metastasis and in healthy thyroid samples. SP and the NK-1R were expressed in all normal and TC samples. In healthy glands, SP was located in follicular cells (nucleus) and colloid and NK-1R in follicular cells (cytoplasm) and stroma. In TC samples, SP was visualized in follicular cells (nucleus and cytoplasm), stroma and colloid and NK-1R in follicular cells (cytoplasm), stroma and colloid. A semiquantitative scoring system (Allred Unit Scoring System) was applied....
Source: European Journal of Histochemistry - March 26, 2020 Category: Biomedical Science Authors: Inmaculada Isorna, Francisco Esteban, Juan Solanellas, Rafael Cove ñas, Miguel Muñoz Source Type: research
Seasonal expressions of SPAG11A and androgen receptor in the epididymis of the wild ground squirrels ( < em > Citellus dauricus < /em > Brandt)
Sperm-associated antigen 11A (SPAG11A) is expressed exclusively in the epididymis, which can specifically regulate sperm maturation. The aim of this study was to investigate the seasonal expressions of beta-defensin (SPAG11A) and androgen receptor (AR) in the epididymis of the wild ground squirrels (Citellus dauricusBrandt). Morphologically, the results showed that epididymis length and weight in the breeding season were significantly higher than those of the non-breeding season. Histologically, the results revealed that enlarged lumen diameters, thickened epithelial and abundant sperm in the breeding season while reduced ...
Source: European Journal of Histochemistry - March 26, 2020 Category: Biomedical Science Source Type: research
The psoriatic shift induced by interleukin 17 is promptly reverted by a specific anti-IL-17A agent in a three-dimensional organotypic model of normal human skin culture
Interleukin 17A (IL-17A), mainly produced by the T helper subclass Th17, plays a key role in the psoriatic plaque formation and progression. The clinical effectiveness of anti-IL-17A agents is documented, but the early and specific mechanisms of their protection are not identified yet. The challenge of the present study is to investigate the possible reversal exerted by a specific anti-IL-17A agent on the psoriatic events induced by IL-17A in a three-dimensional organotypic model of normal human skin. Bioptic skin fragments obtained after aesthetic surgery of healthy women (n=5) were incubated with i) IL-17A biological inh...
Source: European Journal of Histochemistry - March 26, 2020 Category: Biomedical Science Source Type: research
Thrombin regulates the ability of Schwann cells to support neuritogenesis and to maintain the integrity of the nodes of Ranvier
Schwann cells (SC) are characterized by a remarkable plasticity that enables them to promptly respond to nerve injury promoting axonal regeneration. In peripheral nerves after damage SC convert to a repair-promoting phenotype activating a sequence of supportive functions that drive myelin clearance, prevent neuronal death, and help axon growth and guidance. Regeneration of peripheral nerves after damage correlates inversely with thrombin levels. Thrombin is not only the key regulator of the coagulation cascade but also a protease with hormone-like activities that affects various cells of the central and peripheral nervous ...
Source: European Journal of Histochemistry - March 26, 2020 Category: Biomedical Science Source Type: research
Expression of E-cadherin, Ki-67, and p53 in urinary bladder cancer in relation to progression, survival, and recurrence
In this study, we analyzed the tumor tissues of 224 patients with pTa, pT1, and pT2 urinary bladder cancer. We performed a histomorphologic analysis and immunohistochemistry for p53, Ki-67, and E-cadherin, which were selected as markers of the malignant process. For pTa and pT1, univariate analyses of cancer-specific survival (CSS), progression-free survival (PFS), and recurrence-free survival (RFS) were calculated using the Kaplan-Meier method, the log-rank test and Cox regression. Multivariate analysis was performed by a Cox regression analysis. Ki-67 (P<0.001) was significantly associated with CSS, but the highest as...
Source: European Journal of Histochemistry - March 26, 2020 Category: Biomedical Science Source Type: research
Erratum: Correction of Affiliation
This corrects the article " Anastomotic healing in a rat model of peritonitis after non-steroidal anti-inflammatory drug administration " in volume 64(1):3085In the published article “Anastomotic healing in a rat model of peritonitis after non-steroidal anti-inflammatory drug administration” Eur J Histochem 2020;64(1):3085,https://doi.org/10.4081/ejh.2020.3085, ” one affiliation was published incorrectly. The authors apologize for any inconvenience that it may have caused.Roberto Ghiselli,1 Guendalina Lucarini,2 Monica Ortenzi,1 Eleonora Salvolini,3 Stefania Saccomanno,2Fiorenza Orlando,4 Mauro Provinciali,4 Fabio Ca...
Source: European Journal of Histochemistry - February 26, 2020 Category: Biomedical Science Source Type: research
Chronic cypermethrin exposure alters mouse embryonic stem cell growth kinetics, induces Phase II detoxification response and affects pluripotency and differentiation gene expression
Worldwide uncontrolled use of synthetic pyrethroids contaminates water and soil leading to health hazards. Cypermethrin (CYP), the most used pyrethroid, induces detrimental effects on adults and embryos at different stages of development of several vertebrate species. In Mammals, CYP-induced alterations have been previously described in adult somatic cells and in post-implantation embryos. It remains unknown whether CYP has effects during pre-implantation development. Studies to access pre-implantation embryo toxicity are complicated by the restricted number of blastocysts that may be obtained, eitherin vivo orin vitro. Em...
Source: European Journal of Histochemistry - February 17, 2020 Category: Biomedical Science Source Type: research
Immunolocalization of aromatase P450 in the epididymis of Podarcis sicula and Rattus rattus
The goal of this study was to evaluate P450 aromatase localization in the epididymis of two different vertebrates: the lizardPodarcis sicula, a seasonal breeder, andRattus rattus, a continuous breeder. P450 aromatase is a key enzyme involved in the local control of spermatogenesis and steroidogenesis and we proved for the first time that this enzyme is represented in the epididymis of bothP. sicula andR. rattus. In details, P450 aromatase was well represented in epithelial and myoid cells and in the connective tissue ofP. sicula epididymis during the reproductive period; instead, during autumnal resumption this enzyme was ...
Source: European Journal of Histochemistry - January 27, 2020 Category: Biomedical Science Source Type: research
Can the AGE/RAGE/ERK signalling pathway and the epithelial-to-mesenchymal transition interact in the pathogenesis of chronic rhinosinusitis with nasal polyps?
Chronic rhinosinusitis with nasal polyps (CRSwNP) is a persistent sinonasal mucosa inflammatory disease with still unclear pathophysiologic mechanisms that imply events of tissue repair and structural remodelling. Several cascades seem to have a considerable role in the onset and progression of mucosa hyperproliferation in nasal polyps including transforming growth factor β/Small mother against decapentaplegic (TGFβ/Smads), mitogenactivated protein kinases (MAPKs), advanced glycosylation end-products (AGEs) together with epithelial-tomesenchymal transition (EMT). Since many inflammatory mediators are reported to play imp...
Source: European Journal of Histochemistry - January 23, 2020 Category: Biomedical Science Source Type: research
Nuclear factor-kB and nitric oxide synthases in red blood cells: good or bad in obesity? A preliminary study
In conclusion, we have considered the literature that focuses on the omega-6/omega-3 ratio. The ratio changed from the past, especially in people whose diet is strongly westernized worsening the state of health of the patient and leading to an higher incidence of obesity. Our study hypoth esizes that the supplementation could help to restore the correct ratio. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - January 21, 2020 Category: Biomedical Science Source Type: research
Immuno- and glyco-histochemistry as a tool to evaluate the oregano supplemented feed effects in pig gut
This study evaluated the effects of adding an aqueous extract of oregano (OAE) to the diet of two homogenous groups of pigs during the finisher phase. The diets were as follows: control commercial diet (CTR group) and CTR diet supplemented (2 g/kg) with OAE (O group). Samples of ileum and caecum from the two groups were examined by conventional histochemistry to analyze complex carbohydrates and by immunohistochemistry to detect Bcl-2 Associate X protein (BAX), an indicator of oxidative stress. Glyco-histochemistry showed significant differences between the two groups. Immunohistochemistry revealed a lower presence of BAX ...
Source: European Journal of Histochemistry - January 10, 2020 Category: Biomedical Science Source Type: research
Expression, localization and synthesis of small leucine-rich proteoglycans in developing mouse molar tooth germ
The gene expression and protein synthesis of small leucine-rich proteoglycans (SLRPs), including decorin, biglycan, fibromodulin, and lumican, was analyzed in the context of the hypothesis that they are closely related to tooth formation.In situ hybridization, immunohistochemistry, and organ culture with metabolic labeling of [35S] were carried out in mouse first molar tooth germs of different developmental stages using ICR mice at embryonic day (E) 13.5 to postnatal day (P) 7.0. At the bud and cap stage,decorin mRNA was expressed only in the surrounding mesenchyme, but not within the tooth germ.BiglycanmRNA was then expre...
Source: European Journal of Histochemistry - January 10, 2020 Category: Biomedical Science Source Type: research
Anastomotic healing in a rat model of peritonitis after non-steroidal anti-inflammatory drug administration
The tissue inflammatory response can influence the outcome of anastomotic healing. Anastomotic leakage represents a dreadful complication after gastrointestinal surgery, in particular sepsis and intra-abdominal infections impair the restorative process of colic anastomoses. It has been debated whether the administration of non-steroidal anti-inflammatory drugs (NSAIDs) is a risk factor for dehiscence, since many patients receive NSAIDs in the early postoperative period. Our aim was, for the first time, to analyze the morpho-functional effects of postoperative administration of two commonly used NSAIDs, Diclofenac and Ketor...
Source: European Journal of Histochemistry - January 10, 2020 Category: Biomedical Science Source Type: research
