Morphological analysis of the seeds of three pseudocereals by using light microscopy and ESEM-EDS
The seed morphology of three Pseudocereal Grains (PSCg),i.e. quinoa (Chenopodium quinoaWilld,Chenopodiaceae), buckwheat (Fagopyrum esculentumMoench,Polygonaceae) and amaranth (Amaranthus caudatusL.,Amaranthaceae) was studied by light microscopy (LM) and Environmental Scanning Electron Microscopy coupled with Energy Dispersive Spectroscopy (ESEM-EDS). LM was used with visible light to evaluate either unstained sections or sections stained with Azan mixture and with fluorescent light. The aim of the study was to compare the architecture of the three seeds in order to connect their morphology with nutrient localization. The A...
Source: European Journal of Histochemistry - January 10, 2020 Category: Biomedical Science Source Type: research
Immunohistochemical study for relationship between vessel and lymphatic properties and tooth marks in human oral mucosa
Angiogenesis is an important issue related to normal growth and differentiation, and it is a critical issue in the progression of human disease in oral mucosa. Tooth marks occur after clenching the teeth for a long period under muscle tension in the human oral cavity. However, the sites of angiogenesis, cell differentiation and microvessel density are not known for human mucosa with tooth marks. Therefore, we investigated the relationship between the markers of differentiation (Ki-67), angiogenesis (CD31, D2-40, VEGF-A), and marks from teeth in the second molar region using immunohistochemical methods. In addition, we comp...
Source: European Journal of Histochemistry - January 10, 2020 Category: Biomedical Science Source Type: research
A journal of histochemistry as a forum for non-histochemical scientific societies
Histochemical techniques are widely applied in biomedical research and, during the last twenty years, they were among the methods used in more than 590,000 scientific articles in indexed journals. However, a very small percentage of these papers were published in strictly histochemical journals. A possible strategy to widen the audience of the histochemical journals making them attractive to non-histochemist authors might be to publish and make open-access available the proceedings of the meetings and conferences of valued scientific societies whose fellows use microscopy and histochemistry in their experimental activity. ...
Source: European Journal of Histochemistry - December 23, 2019 Category: Biomedical Science Source Type: research
Alcian blue staining to track the intracellular fate of hyaluronic-acid-based nanoparticles at transmission electron microscopy
In this study, the critical-electrolyte-concentration Alcian Blue method set up by Schofieldet al. in 1975 was applied to track hyaluronic-acid-based NPs in muscle cellsin vitro. This long-established histochemical method proved to be a powerful tool allowing to identify not only whole NPs while entering cells and moving into the cytoplasm, but also their remnants following lysosomal degradation and extrusion. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - December 12, 2019 Category: Biomedical Science Source Type: research
Downregulation of SRPK2 promotes cell cycle arrest though E2F1 in non-small cell lung cancer
Serine-arginine protein kinase (SRPK) belongs to a class of cell cycle regulating kinases that can phosphorylate proteins containing serine/arginine-Rich (SR) regions. SR proteins are a family of RNA binding phosphoproteins that control both constitutive and alternative pre-mRNA splicing events. However, little is known about their role in non-small cell lung cancer (NSCLC). In the present study, we found that serine-arginine protein kinase 2 (SRPK2) expression was upregulated in NSCLC tissues compared with adjacent normal tissues. Kaplan-Meier curve analyses showed that the overall survival time of NSCLC patients with hig...
Source: European Journal of Histochemistry - December 11, 2019 Category: Biomedical Science Source Type: research
Bleaching melanin in formalin-fixed and paraffin-embedded melanoma specimens using visible light: a pilot study
In conclusion, visible light can be effectively exploited to bleach pigmented melanoma FFPE sections prior to perform routine histochemical and immunohistochemical stains. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - October 31, 2019 Category: Biomedical Science Source Type: research
Increased expression of STAT3 and SOCS3 in placenta from hyperglycemic rats
Signal transducer and activator of transcription 3 (STAT3) is a transcription factor that is activated by interleukin (IL)-6 and IL-10 that generate nearly opposing responses. The suppressor of cytokine signaling 3 (SOCS3) is the negative regulator of STAT3 and plays an important role in the negative regulation of the inflammatory process. Evidence has shown the importance of STAT3 and SOCS3 during implantation and normal pregnancy. However, little is known about the relationship of both factors under hyperglycemic condition. The aim of this study was to evaluate the placenta regions exhibiting immunopositivity for STAT3 a...
Source: European Journal of Histochemistry - October 30, 2019 Category: Biomedical Science Source Type: research
Erythrocytes of the Rhesus and Cynomolgus Monkeys
The monographErythrocytes of the Rhesus and Cynomolgus Monkeys is devoted to the cytologic, quantitative, generative normal and abnormal presentations of the erythrocytes in these two species of primates... (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - October 22, 2019 Category: Biomedical Science Source Type: research
Bradykinin and noradrenaline preconditioning influences level of antioxidant enzymes SOD, CuZn-SOD, Mn-SOD and catalase in the white matter of spinal cord in rabbits after ischemia/reperfusion
The aim of present work is to assess the effects of bradykinin (Br) or noradrenaline (Nor) preconditioning to the levels of antioxidant enzymes: superoxide dismutase (SOD), copper, zinc superoxide dismutase (CuZn-SOD), manganese superoxide dismutase (Mn-SOD) and catalase in ischemia/reperfusion (I/R) model in the rabbit spinal cord white matter as well as effect on glial fibrillary acidic protein (GFAP) and ubiquitin immunoreaction in glial cells. Rabbits were preconditioned by intraperitoneal single dose of Br or Nor 48 h prior to 20 min of ischemia followed by 24 or 48 h of reperfusion. White matter of L3-L6 spinal cord ...
Source: European Journal of Histochemistry - October 18, 2019 Category: Biomedical Science Source Type: research
Immunofluorescence characterization of innervation and nerve-immune cell interactions in mouse lymph nodes
The peripheral nervous system communicates specifically with the immune system via local interactions. These interactions include the “hardwiring” of sympathetic/parasympathetic (efferent) and sensory nerves (afferent) to primary (e.g., thymus and bone marrow) and secondary (e.g., lymph node, spleen, and gut-associated lymphoid tissue) lymphoid tissue/organs. To gain a better understanding of this bidirectional interaction/crosstalk between the two systems, we have investigated the distribution of nerve fibres and PNS-immune cell associationsin situ in the mouse lymph node by using immunofluorescent staining and confoc...
Source: European Journal of Histochemistry - October 18, 2019 Category: Biomedical Science Source Type: research
Digital PCR - Methods and Protocols
Sensitivity, reproducibility, precision and accuracy are recurring words in this book. Indeed, digital PCR (dPCR) represents a major step forward in the quantification of nucleic acids. In this field, for many years it has been used the quantitative PCR (qPCR), a technology that allows a real-time monitoring of DNA amplification which has proved useful for obtaining a relative measurement, but that only indirectly, through the use of reference standards, provides an absolute quantification.... (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - October 18, 2019 Category: Biomedical Science Source Type: research
Bradykinin and noradrenaline preconditioning influences level of antioxidant enzymes SOD, CuZn-SOD, Mn-SOD and catalase in the white matter of spinal cord in rabbits after ischemia/reperfusion
The aim of present work is to assess the effects of bradykinin (Br) or noradrenaline (Nor) preconditioning to the levels of antioxidant enzymes: superoxide dismutase (SOD), copper, zinc superoxide dismutase (CuZn-SOD), manganese superoxide dismutase (Mn-SOD) and catalase in ischemia/reperfusion (I/R) model in the rabbit spinal cord white matter as well as effect on glial fibrillary acidic protein (GFAP) and ubiquitin immunoreaction in glial cells. Rabbits were preconditioned by intraperitoneal single dose of Br or Nor 48 h prior to 20 min of ischemia followed by 24 or 48 h of reperfusion. White matter of L3-L6 spinal cord ...
Source: European Journal of Histochemistry - October 18, 2019 Category: Biomedical Science Source Type: research
