1954-2024: 70 years of histochemical research with the European Journal of Histochemistry
This Editorial celebrates the 70th anniversary of theEuropean Journal of Histochemistry since its foundation asRivista di Istochimica Normale e Patologica, and introduces a Special Collection of selected articles on the application of the histochemical approach for investigating cell biological features and processes in animals and plants, and under diseased conditions. The year 2024 is a special one for histochemists, as 100 years ago J.W. Robert Feulgen and H. Rossenbeck introduced the histochemical procedure for the specific stoichiometric staining of DNA in histological samples: to commemorate this influential publicat...
Source: European Journal of Histochemistry - January 29, 2024 Category: Biomedical Science Authors: Carlo Pellicciari, Marco Biggiogera, Manuela Malatesta Source Type: research
Umbilical cord mesenchymal stem cells inhibited inflammation of bronchial epithelial cells by regulating Hedgehog pathway
In conclusion, UCMSCs have protective effects on TGF-β1-induced inflammation in human bronchial epithelial cells by regulating the Hedgehog pathway. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - December 12, 2023 Category: Biomedical Science Authors: Qiong Lin, Tianxing Yu, Xiaohua Li, Xin Lin, Yong Fan, Liyu Xu Source Type: research
Proceedings of the 33rd National Conference of the Italian Group for the Study of Neuromorphology “Gruppo Italiano per lo Studio della Neuromorfologia” G.I.S.N., Verona, November 24-25, 2023
(Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - November 23, 2023 Category: Biomedical Science Authors: Conference Chair: Raffaella Mariotti Source Type: research
SNORA38B promotes proliferation, migration, invasion and epithelial-mesenchymal transition of gallbladder cancer cells < em > via < /em > activating TGF- β/Smad2/3 signaling
Evidence has shown that small nucleolar RNAs (snoRNAs) participate in the tumorigenesis in multiple cancers, including gallbladder cancer (GBC). Our results showed that SNORA38B level was increased in GBC tissues compared to adjacent normal tissues. Thus, this research aimed to explore the role and molecular mechanisms of SNORA38B in GBC. SNORA38B level between normal and GBC tissues was evaluated by RT-qPCR. Cell proliferation, apoptosis, migration, and invasion were tested by EdU assay, TUNEL staining and transwell assay, respectively on human intrahepatic biliary epithelial cells (HIBEpiCs) and the GBC cell lines, NOZ a...
Source: European Journal of Histochemistry - October 2, 2023 Category: Biomedical Science Authors: Yiyu Qin, Jian Li, Hongchao Han, Yongliang Zheng, Haiming Lei, Yang Zhou, Hongyan Wu, Guozhe Zhang, Xiang Chen, Zhengping Chen Source Type: research
Investigation of seasonal changes in lipid synthesis and metabolism-related genes in the oviduct of Chinese brown frog ( < em > Rana dybowskii < /em > )
A peculiar physiological characteristic of the Chinese brown frog (Rana dybowskii) is that its oviduct dilates during pre-brumation rather than during the breeding season. This research aimed to examine the expression of genes connected with lipid synthesis and metabolism in the oviduct ofR. dybowskii during both the breeding season and pre-brumation. We observed significant changes in the weight and size of the oviduct between the breeding season and pre-brumation. Furthermore, compared to the breeding season, pre-brumation exhibited significantly lower triglyceride content and a marked increase in free fatty acid content...
Source: European Journal of Histochemistry - October 2, 2023 Category: Biomedical Science Authors: Yankun Wang, Yuning Liu, Yawei Wang, Ao Zhang, Wenqian Xie, Haolin Zhang, Qiang Weng, Meiyu Xu Source Type: research
Curculigoside inhibits osteoarthritis < em > via < /em > the regulation of NLRP3 pathway
In conclusion, curculigoside exhibits beneficial effect on cartilageviathe inhibition of NLRP3 pathway. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - October 2, 2023 Category: Biomedical Science Authors: Xufei Wang, Yinlian Liu, Yongnian Zhou, Yang Zhou, Yueping Li Source Type: research
Pre-treatment with galectin-1 attenuates lipopolysaccharide-induced myocarditis by regulating the Nrf2 pathway
In conclusion, pre-treatment with Gal-1 exhibited cardioprotective effects in myocarditisvia anti-inflammatory and antioxidant functions, and the mechanism may relate to the Nrf2 pathway, which offered new solid evidence for the use of Gal-1 in preventing myocarditis. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - October 2, 2023 Category: Biomedical Science Authors: Liying Shen, Kongjie Lu, Zhenfeng Chen, Yingwei Zhu, Cong Zhang, Li Zhang Source Type: research
Regulatory roles of ACSL5 in the anti-tumor function of palmitic acid (C16:0) < em > via < /em > the ERK signaling pathway
Previous studies have highlighted the susceptibility of cancer to perturbations in lipid metabolism. In particular, C16:0 has emerged as a promising novel treatment for hepatocellular carcinoma. In our study, we investigated the levels of C16:0 in the serum of non-small lung cancer patients were significant downregulation compared to healthy individuals (n=10; p<0.05). Moreover, ourin vitro experiments using A549 cells demonstrated that C16:0 effectively inhibited proliferation, apoptosis, migration, and invasion. Despite these promising results, its pathogenesis remains poorly understood. CCK-8 assay, annexin V-FITC/PI...
Source: European Journal of Histochemistry - October 2, 2023 Category: Biomedical Science Authors: Jiapei Lv, Wang Yanting, Shan Wei Source Type: research
Expression and role of cocaine-amphetamine regulated transcript (CART) in the proliferation of biliary epithelium
Cholangiocytes, the epithelial cells that line the biliary tree, can proliferate under the stimulation of several factors through both autocrine and paracrine pathways. The cocaine-amphetamine-regulated-transcript (CART) peptide has several physiological functions, and it is widely expressed in several organs. CART increases the survival of hippocampal neurons by upregulating brain-derived neurotrophic factor (BDNF), whose expression has been correlated to the proliferation rate of cholangiocytes. In the present study, we aimed to evaluate the expression of CART and its role in modulating cholangiocyte proliferation in hea...
Source: European Journal of Histochemistry - October 2, 2023 Category: Biomedical Science Authors: Arianna Casini, Giorgio Vivacqua, Rosa Vaccaro, Anastasia Renzi, Stefano Leone, Luigi Pannarale, Antonio Franchitto, Paolo Onori, Romina Mancinelli, Eugenio Gaudio Source Type: research
Ozone and procaine increase secretion of platelet-derived factors in platelet-rich plasma
Platelet-rich plasma (PRP) is gaining more and more attention in regenerative medicine as an innovative and efficient therapeutic approach. The regenerative properties of PRP rely on the numerous bioactive molecules released by the platelets: growth factors are involved in proliferation and differentiation of endothelial cells and fibroblasts, angiogenesis and extracellular matrix formation, while cytokines are mainly involved in immune cell recruitment and inflammation modulation. Attempts are ongoing to improve the therapeutic potential of PRP by combining it with agents able to promote regenerative processes. Two intere...
Source: European Journal of Histochemistry - October 2, 2023 Category: Biomedical Science Authors: Chiara Rita Inguscio, Barbara Cisterna, Maria Assunta Lacavalla, Franco Donati, Osvaldo Angelini, Gabriele Tabaracci, Manuela Malatesta Source Type: research
Overexpression of hsa_circ_0001861 inhibits pulmonary fibrosis through targeting miR-296-5p/BCL-2 binding component 3 axis
Pulmonary fibrosis is a progressive lung disorder. Evidence has shown that hsa_circular (circ)RNA_0001861 is dysregulated in pulmonary fibrosis. However, the detailed function of hsa_circRNA_0001861 in pulmonary fibrosis remains unexplored. To investigate the function of hsa_circRNA_0001861 in pulmonary fibrosis, human pulmonary fibroblastsin vitro were used, and cell counting kit-8 (CCK-8) and 5-ethynyl-2 ’-deoxyuridine (EdU) staining were performed to assess cell viability and proliferation, respectively. Western blot analysis and reverse transcription-quantitative PCR (RT-qPCR) were used to evaluate protein and mRNA l...
Source: European Journal of Histochemistry - October 2, 2023 Category: Biomedical Science Authors: Tao Wu, Shikui Wu, Hailu Jiao, Jun Feng, Xiang Zeng Source Type: research
Management of autofluorescence in formaldehyde-fixed myocardium: choosing the right treatment
Autofluorescence (AF) poses challenges for detecting proteins of interestin situ when employing immunofluorescence (IF) microscopy. This interference is particularly pronounced in strongly autofluorescent tissues such as myocardium, where tissue AF can be comparable to IF. Although various histochemical methods have been developed to achieve effective AF suppression in different types of tissue, their applications on myocardial samples have not been well validated. Due to inconsistency across different autofluorescent structures in sometypes of tissue, it is unclear if these methods can effectively suppress AF across all a...
Source: European Journal of Histochemistry - October 2, 2023 Category: Biomedical Science Authors: Zhao Zhang, Hongming Fan, William Richardson, Bruce Z. Gao, Tong Ye Source Type: research
Seasonal patterns of prolactin, prolactin receptor, and STAT5 expression in the ovaries of wild ground squirrels ( < em > Citellus dauricus < /em > Brandt)
This study aimed to examine the immunolocalization and expression patterns of PRL, prolactin receptor (PRLR), and signal transducer and activator of transcription 5 (STAT5) in the ovaries of wild ground squirrels during both breeding and non-breeding periods. Significant seasonal variations were observed in ovarian weights, with higher values during the breeding season and relatively lower values during the nonbreeding season. PRL, PRLR, STAT5, and p-STAT5 were immunolocalized in granulosa cells and luteal cells during the breeding season, whereas they were exclusively found in granulosa cells during the non-breeding seaso...
Source: European Journal of Histochemistry - October 2, 2023 Category: Biomedical Science Authors: Qingjing Gao, Wenqian Xie, Wenjing Lu, Yuning Liu, Haolin Zhang, Yingying Han, Qiang Weng Source Type: research
Expression and localization of & #945; < sub > 2A < /sub > -adrenergic receptor in the rat post-natal developing cochlea
This study provides direct evidence for the expression of α2A-AR in HCs, SGNs, and the SV of the cochlea, indicating that norepinephrine might play a vital role in hearing function within the cochlea through α2A-AR. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - August 7, 2023 Category: Biomedical Science Authors: Chaoyong Tian, Yang Yang, Yao Li, Fei Sun, Juan Qu, Dingjun Zha Source Type: research
Crocin exerts anti-tumor effect in colon cancer cells < em > via < /em > repressing the JAK pathway
Crocin has been reported to have therapeutic effects on multiple cancers including colon cancer, but its specific mechanism is still ambiguous and needs to be further explored. Human colorectal adenocarcinoma cells (HCT-116) and human normal colonic epithelial cells (CCD841) were first treated with increasing concentrations of crocin. Subsequently, with 150 and 200 μM of crocin, the cell vitality was examined by cell counting kit 8. Cell apoptosis and proliferation were tested by TUNEL staining and colony formation assay, respectively. The expression of Ki-67 was assessed by immunofluorescence. Enzyme-linked immunosorbent...
Source: European Journal of Histochemistry - July 26, 2023 Category: Biomedical Science Authors: Hui Yang, Yunlong Zhang, Desheng Zhang, Liping Qian, Tianxing Yang, Xiaocheng Wu Source Type: research
