Knockdown of ARHGAP30 inhibits ovarian cancer cell proliferation, migration, and invasiveness by suppressing the PI3K/AKT/mTOR signaling pathway
In conclusion, ARHGAP30 regulated the PI3K/AKT/mTOR pathway to promote progression of OC. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - March 20, 2023 Category: Biomedical Science Authors: Xiaoyan Chu, Jun Lou, Yun Yi, Linlin Zhong, Ouping Huang Source Type: research
< b > RETRACTION < /b > : Intrinsic innervation and dopaminergic markers after experimental denervation in rat thymus
On behalf of the coauthors and with much regret, I must retract our publication entitled “Intrinsic innervation and dopaminergic markers after experimental denervation in rat thymus” published inEuropean Journal of Histochemistry 2010;54(2):e17 for the following reason:Unfortunately, now, after thirteen years, we have realized that some microphotographs published in the paper have been processed to improve the presentation of the images.The three surviving authors of the paper agree that the processing of the presentation images is against the COPE Ethical Editorial Standard, although the presentation images do not alt...
Source: European Journal of Histochemistry - March 20, 2023 Category: Biomedical Science Authors: Fiorenzo Mignini, Maurizio Sabbatini, Vito D ' Andrea, Carlo Cavallotti Source Type: research
The role of miR-143-3p/FNDC1 axis on the progression of non-small cell lung cancer
In conclusion, FNDC1 promotes the malignant prototypes of NSCLC cells. miR-143-3p is a negative regulator of FNDC1 in NSCLC cells, which may serve as a promising therapeutic target in NSCLC. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - March 20, 2023 Category: Biomedical Science Authors: Zhanshu Ma, Qi Gao, Wenjing Xin, Lei Wang, Yan Chen, Chang Su, Songyan Gao, Ruiling Sun Source Type: research
Clinicopathological assessment of PD-1/PD-L1 immune checkpoint expression in desmoid tumors
This study aimed to determine the expression status of the programmed death-1/programmed death ligand 1 (PD1/PD-L1) immune checkpoint mechanism in DTs. The study included patients with DTs (n=9) treated at our institution between April 2006 and December 2012. Immunostaining for CD4, CD8, PD-1, PD-L1, interleukin-2 (IL-2), and interferon-gamma (IFN- γ) was performed on pathological specimens harvested during the biopsy. The positivity rate of each immune component was calculated as the number of positive cells/total cells. The positivity rate was quantified and correlations between the positivity rates of each immune molec...
Source: European Journal of Histochemistry - March 20, 2023 Category: Biomedical Science Authors: Kazuhiko Hashimoto, Shunji Nishimura, Yu Shinyashiki, Tomohiko Ito, Ryosuke Kakinoki, Masao Akagi Source Type: research
Molecules involved in the sperm interaction in the human uterine tube: a histochemical and immunohistochemical approach
In humans, even where millions of spermatozoa are deposited upon ejaculation in the vagina, only a few thousand enter the uterine tube (UT). Sperm transiently adhere to the epithelial cells lining the isthmus reservoir, and this interaction is essential in coordinating the availability of functional spermatozoa for fertilization. The binding of spermatozoa to the UT epithelium (mucosa) occurs due to interactions between cell-adhesion molecules on the cell surfaces of both the sperm and the epithelial cell. However, in humans, there is little information about the molecules involved. The aim of this study was to perform a h...
Source: European Journal of Histochemistry - March 20, 2023 Category: Biomedical Science Authors: David Cajas , Emanuel Guajardo, Sergio Jara-Rosales, Claudio Nu ñez, Renato Vargas, Victor Carriel, Antonio Campos, Luis Milla, Pedro Orihuela, Carlos Godoy-Guzman Source Type: research
Effects of piperlonguminine on lung injury in severe acute pancreatitis < em > via < /em > the TLR4/NF- κB pathway
Acute pancreatitis is an inflammatory response in the pancreas, involving activation of pancreatic enzymes. Severe acute pancreatitis (SAP) often causes systemic complications that affect distant organs, including the lungs. The aim of this study was to explore the therapeutic potential of piperlonguminine on SAP-induced lung injury in rat models. Acute pancreatitis was induced in rats by repetitive injections with 4% sodium taurocholate. Histological examination and biochemical assays were used to assess the severity of lung injury, including tissue damage, and levels of nicotinamide adenine dinucleotide phosphate (NADPH)...
Source: European Journal of Histochemistry - March 20, 2023 Category: Biomedical Science Authors: Qian Hu, Ran Tao, Xiaoyun Hu, Haibo Wu, Jianjun Xu Source Type: research
Nrf2 as a novel diagnostic biomarker for papillary thyroid carcinoma
In conclusion, Nrf2 displays a high abundance expression in human PTC, which leads to the higher expression of downstream transcriptional proteins: HO-1 and NQO1. Moreover, Nrf2 can be used as an extra biomarker for differential diagnosis of PTC and a predictive biomarker for lymph node metastasis of PTC. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - March 20, 2023 Category: Biomedical Science Authors: Zhiyang Wang, Jing Li, Ziwei Liu, Ling Yue Source Type: research
The contribution of immunohistochemistry to the development of hydrogels for skin repair and regeneration
Hydrogels based on various polymeric materials have been successfully developed in recent years for a variety of skin applications. Several studies have shown that hydrogels with regenerative, antibacterial, and antiinflammatory properties can provide faster and better healing outcomes, particularly in chronic diseases where the normal physiological healing process is significantly hampered. Various experimental tests are typically performed to assess these materials ' ability to promote angiogenesis, re-epithelialization, and the production and maturation of new extracellular matrix. Immunohistochemistry is important in t...
Source: European Journal of Histochemistry - February 23, 2023 Category: Biomedical Science Authors: Flavia Carton Source Type: research
Effects of artificial light with different spectral composition on eye axial growth in juvenile guinea pigs
The purpose of the study was to investigate the effect of artificial light with different spectral composition and distribution on axial growth in guinea pigs. Three-week-old guinea pigs were randomly assigned to groups exposed to natural light, low color temperature light-emitting diode (LED) light, two full spectrum artificial lights (E light and Julia light) and blue light filtered light with the same intensity. Axial lengths of guinea pigs ’ eyes were measured by A-scan ultrasonography prior to the experiment and every 2 weeks during the experiment. After light exposure for 12 weeks, retinal dopamine (DA), dihydroxy-...
Source: European Journal of Histochemistry - February 6, 2023 Category: Biomedical Science Authors: Xinyu Xu, Jiayu Shi, Chuanwei Zhang, Lixin Shi, Yujie Bai, Wei Shi, Yuliang Wang Source Type: research
Neurokinin-1 receptor is highly expressed in cervical cancer and its antagonist induces cervical cancer cell apoptosis
In conclusion, these findings suggest that NK1R plays an oncogenic role in cervical cancer and is a promising target for cervical cancer therapy. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - January 11, 2023 Category: Biomedical Science Authors: Liyun Guan, Shifa Yuan, Jing Ma, Hong Liu, Lizhen Huang, Fengzhen Zhang Source Type: research
The Qi-Bang-Yi-Shen formula ameliorates renal dysfunction and fibrosis in rats with diabetic kidney disease < em > via < /em > regulating PI3K/AKT, ERK and PPAR γ signaling pathways
In this study, we found that QBF or Los treatment could decrease serum BUN, CRE, UA levels and reduce urine albumin-to-creatinine ratio (ACR) in DKD rats. Additionally, QBF or Los treatment obviously inhibited glomerular mesangial expansion and glomerular fibrosis, attenuated glomerular injury in kidney tissues of DKD rats. Moreover, QBF or Los treatment significantly reduced PI3K, AKT and ERK1/2 protein expressions, but increased PPAR γ level in kidney tissues of DKD rats. As expected, combined treatment of QBF and Los could exert enhanced reno-protective effects compared with the single treatment. Collectively, combinat...
Source: European Journal of Histochemistry - January 2, 2023 Category: Biomedical Science Authors: Zhi Wang, Guihua Jian, Teng Chen, Yiping Chen, Junhui Li, Niansong Wang Source Type: research
In memoriam of Prof. Stan Fakan
(Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - January 2, 2023 Category: Biomedical Science Authors: Marco Biggiogera, Manuela Malatesta Source Type: research
LINC00926 is involved in hypoxia-induced vascular endothelial cell dysfunction < em > via < /em > miR-3194-5p regulating JAK1/STAT3 signaling pathway
Vascular endothelial cell (VEC) dysfunction is associated with the development of coronary heart disease (CHD). Long intergenic non-protein coding RNA 926 (LINC00926), a kind of long noncoding RNA (lncRNA), has been found to be abnormally expressed in CHD patients. However, the biological role of LINC00926 has not been reported. In our research, we intended to explore the regulatory mechanism of LINC00926 in hypoxia-exposed HUVEC cells (HUVECs). In ourin vitro study, HUVECs were exposed under hypoxic conditions (5% O2) for 24 h. RT-qPCR and Western blotting assay were used to detect the mRNA and protein levels. CCK-8 assay...
Source: European Journal of Histochemistry - January 2, 2023 Category: Biomedical Science Authors: Yong Jiang, Chun-hui Xu, Ying Zhao, Yun-han Ji, Xin-tao Wang, Ying Liu Source Type: research
Growth hormone inhibits the JAK/STAT3 pathway by regulating SOCS1 in endometrial cells < em > in vitro < /em > : a clue to enhance endometrial receptivity in recurrent implantation failure
In this study, using immunohistochemistry and Western blot, we demonstrated that the expression of leukemia inhibitory factor (LIF), Janus kinase 1 (JAK1), and signal transducer and activator of transcription 3 (STAT3) was increased, while that of suppressor of cytokine signaling 1 (SOCS1) was decreased in RIF patients. Growth hormone (GH) administration proved to have positive effects on embryo implantation in RIF patients, but the action mechanism of GH has not been elucidated yet. To this aim, we studied the effects of GH on the proliferationin vitro of endometrial adenocarcinoma Ishikawa cells. GH stimulated the expres...
Source: European Journal of Histochemistry - December 22, 2022 Category: Biomedical Science Authors: Haixia Li, Ning Sun, Yaqiao Zhu, Wei Wang, Meihong Cai, Xiaohuan Luo, Wei Xia, Song Quan Source Type: research
Phosphorylation mutation impairs the promoting effect of spastin on neurite outgrowth without affecting its microtubule severing ability
In conclusion, the data suggest that mutations at multiple phosphorylation sites of spastin do not impair its microtubule cleavage ability in COS7 cells, but reduce its ability to promote neurite outgrowth in rat hippocampal neurons. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - December 22, 2022 Category: Biomedical Science Authors: Yunlong Zhang, Xin He, Jianyu Zou, Jie Yang, Ao Ma, Minghui Tan Source Type: research
