alpha2,3 sialic acid processing enzymes expression in gastric cancer tissues reveals that ST3Gal3 but not Neu3 are associated with Lauren's classification, angiolymphatic invasion and histological grade
This study retrospectively characterized Neu3 and ST3Gal3 expression by immunohistochemistry in 71 paraffin-embedded GC tissue specimens and analyzed the relationship between their expression and the clinicopathological parameters. Neu3 expression was markedly increased in GC tissues compared with non-tumoral tissues (p<0.0001). Intratumoral ST3Gal3 staining was significantly associated with intestinal subtype (p=0.0042) and was negatively associated with angiolymphatic invasion (p=0.0002) and higher histological grade G3 (p=0.0066). Multivariate analysis revealed that ST3Gal3 positivity is able to predict Lauren ' s cl...
Source: European Journal of Histochemistry - September 20, 2022 Category: Biomedical Science Authors: Michael W.L. Quirino, Amanda P.B. Albuquerque, Maria F.D. de Souza, Ant ônio F. da Silva Filho, Mário R. Martins, Maira G. da Rocha Pitta, Michelly C. Pereira, Moacyr J.B. de Melo Rêgo Source Type: research
Imaging and spectral analysis of autofluorescence patterns in larval head structures of mosquito vectors
Autofluorescence (AF) in mosquitoes is currently poorly explored, despite its great potential as a marker of body structures and biological functions. Here, for the first time AF in larval heads of two mosquitoes of key public health importance,Aedes albopictus andCulex pipiens, is studied using fluorescence imaging and spectrofluorometry, similarly to a label-free histochemical approach. In generally conserved distribution patterns, AF shows differences between mouth brushes and antennae of the two species. The blue AF ascribable to resilin at the antennal bases, more extended inCx. pipiens, suggests a potential need to s...
Source: European Journal of Histochemistry - September 20, 2022 Category: Biomedical Science Authors: Francesca Scolari, Alessandro Girella, Anna Cleta Croce Source Type: research
Is mastocytic colitis a specific clinical-pathological entity?
The number of intestinal mast cells (MC) is increased in several types of colitis, but the mucosa of patients with chronic non-bloody diarrhea has not been studied. The current study sought to determine the relationship between MC counts and degranulation and the severity of symptoms in patients with chronic loose stools. Following a negative laboratory workup for the most common causes of chronic diarrhea, patients with chronic non-bloody loose stools were included in the study. Patients with macroscopic evidence of inflammation or organic disease were excluded after endoscopy with biopsies. Biopsies from the 179 patients...
Source: European Journal of Histochemistry - September 20, 2022 Category: Biomedical Science Authors: Filippo Vernia, Tiziana Tatti, Stefano Necozione, Annalisa Capannolo, Nicola Cesaro, Marco Magistroni, Marco Valvano, Simona Pompili, Roberta Sferra, Antonella Vetuschi, Giovanni Latella Source Type: research
Polydatin reverses oxidation low lipoprotein (oxLDL)-induced apoptosis of human umbilical vein endothelial cells < em > via < /em > regulating the miR-26a-5p/BID axis
Atherosclerosis is a disease in which lipids and inflammatory factors accumulate on the walls of arteries, forming plaques that eventually block the flow of blood. Polydatin was derived from plant knotweed, which could play an important role in inhibiting the progression of atherosclerosis. However, the mechanism by which polydatin regulates the genesis and development of atherosclerosis remains unclear. To detect the function of polydatin in atherosclerosis, the proliferation, apoptosis and migration of human umbilical vein endothelial cells (HUVECs) was detected using 5-ethynyl-2 ’-deoxyuridine staining, flow cytometry...
Source: European Journal of Histochemistry - September 20, 2022 Category: Biomedical Science Authors: Dajie Wang, Zhaofeng Zhou, Liang Yuan Source Type: research
Long non-coding RNA WAC antisense RNA 1 mediates hepatitis B virus replication < em > in vitro < /em > by reinforcing miR-192-5p/ATG7-induced autophagy
Long non-coding RNA WAC antisense RNA 1 (lncRNA WAC-AS1) is involved in the replication of the hepatitis B virus (HBV). The purpose of this study was to determine its functions and specific mechanism. The levels of lncRNA WAC-AS1, RNA (miR)-192-5p and were examined in serum of HBV-infected patients and in HepG2.2.15 cells using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and Western blotting. Using the database starBase, the target binding sites of lncRNA WAC-AS1 and miR-192-5p were predicted and confirmed by dual-luciferase reporter assay and RNA pull-down assay. The expression of pgRNA and HBV ...
Source: European Journal of Histochemistry - September 2, 2022 Category: Biomedical Science Authors: Minkai Cao, Deping Yuan, Hongxiu Jiang, Guanlun Zhou, Chao Chen, Guorong Han Source Type: research
MiR-550a-3p restores damaged vascular smooth muscle cells by inhibiting thrombomodulin in an < em > in vitro < /em > atherosclerosis model
Thrombomodulin (TM) is involved in the pathological process of atherosclerosis; however, the underlying mechanism remains unclear. Oxidised low-density lipoprotein (Ox-LDL; 100 μg/mL) was used to induce human vascular smooth muscle cells (HVSMCs) into a stable atherosclerotic cell model. The expression levels of miR-550a-3p and TM were detected by real-time reverse transcription-polymerase chain reaction. Cell proliferation was estimated using CCK8 and EDU assays. Wound s cratch and transwell assays were used to measure the ability of cells to invade and migrate. Propidium iodide fluorescence-activated cell sorting was us...
Source: European Journal of Histochemistry - July 19, 2022 Category: Biomedical Science Authors: Shiyuan Chen, Longfei Zhang, Benchi Feng, Wei Wang, Delang Liu, Xinyu Zhao, Chaowen Yu, Xiaogao Wang, Yong Gao Source Type: research
Clinicopathological assessment of cancer/testis antigens NY ‑ESO‑1 and MAGE‑A4 in osteosarcoma
The cancer/testis antigens (CTAs), New York esophageal squamous cell carcinoma-1 (NY-ESO-1) and melanoma antigen gene (MAGE)-A4 are normally restricted to male germ cells but are aberrantly expressed in several cancers. Considering the limited information regarding their significance in osteosarcoma (OS), the purpose of this study was to determine the clinical significance of NY-ESO-1 and MAGE-A4 expression in OS. Nine patients with OS treated at Kindai University Hospital were included in the study. The median age was 27 years, and median follow-up period was 40 months. The specimens obtained at the time of biopsy were us...
Source: European Journal of Histochemistry - June 21, 2022 Category: Biomedical Science Authors: Kazuhiko Hashimoto, Shunji Nishimura, Tomohiko Ito, Naohiro Oka, Ryosuke Kakinoki, Masao Akagi Source Type: research
Extracellular vesicles from bone mesenchymal stem cells transport microRNA-206 into osteosarcoma cells and target NRSN2 to block the ERK1/2-Bcl-xL signaling pathway
This study investigated the mechanism of BMSCs-EVs on OS. BMSC surface antigens and adipogenic and osteogenic differentiation were detected by flow cytometry, and oil red O and alizarin red staining. EVs were isolated from BMSCs by differential centrifugation and identified by transmission electron microscopy, nanoparticle tracking analysis, and Western blot (WB). miR-206 and neurensin-2 (NRSN2) levels in human osteoblast hFOB 1.19 or OS cells (143B, MG-63, Saos2, HOS) were detected by RT-qPCR. Human OS cells with lower miR-206 levels were selected and treated with BMSCs-EVs or pSUPER-NRSN2. The uptake of EVs by 143B cells...
Source: European Journal of Histochemistry - June 21, 2022 Category: Biomedical Science Authors: Alimu Keremu, Pazila Aila, Aikebaier Tusun , Maimaitiaili Abulikemu, Xiaoguang Zou Source Type: research
Transcription factor nuclear factor erythroid 2 p45-related factor 2 (NRF2) ameliorates sepsis-associated acute kidney injury by maintaining mitochondrial homeostasis and improving the mitochondrial function
Mitochondrial dysfunction has a role in sepsis-associated acute kidney injury (S-AKI), so the restoration of normal mitochondrial homeostasis may be an effective treatment strategy. Transcription factor nuclear factor erythroid 2 p45-related factor 2 (NRF2) is a main regulator of cell-redox homeostasis, and recent studies reported that NRF2 activation helped to preserve mitochondrial morphology and function under conditions of stress. However, the role of NRF2 in the process of S-AKI is still not well understood. The present study investigated whether NRF2 regulates mitochondrial homeostasis and influences mitochondrial fu...
Source: European Journal of Histochemistry - June 21, 2022 Category: Biomedical Science Authors: Zhijiang Chen, Huili Wang, Bin Hu, Xinxin Chen, Meiyu Zheng, Lili Liang, Juanjuan Lyu, Qiyi Zeng Source Type: research
In memoriam of Prof. GianCarlo Panzica
(Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - June 21, 2022 Category: Biomedical Science Authors: Editor in Chief Source Type: research
Immunofluorescence signal intensity measurements as a semi-quantitative tool to assess sarcoglycan complex expression in muscle biopsy
This study provides a sensitive tool for semi-quantification of residual amount of sarcoglycans in patients affected by sarcoglycanopathies, based on immunofluorescence staining on skeletal muscle sections, image acquisition and software elaboration. We applied this method to eleven sarcoglycanopathies, seven Becker muscular dystrophies and four age-matched controls. Fluorescence data analysed in patients and compared to age-matched controls showed a significant reduction of the mutated sarcoglycan expression and a variable reduction of the other sarcoglycans. Fluorescence normalized data analysed in relation to the age of...
Source: European Journal of Histochemistry - June 21, 2022 Category: Biomedical Science Authors: Simona Zanotti, Francesca Magri, Francesca Poggetti, Michela Ripolone, Daniele Velardo, Francesco Fortunato, Patrizia Ciscato, Maurizio Moggio, Stefania Corti, Giacomo Pietro Comi, Monica Sciacco Source Type: research
Inhibition of APLN suppresses cell proliferation and migration and promotes cell apoptosis in esophageal cancer cells < em > in vitro < /em > , through activating PI3K/mTOR signaling pathway
Esophageal cancer is the sixth leading cause of cancer mortalities globally with a high incidence rate. Apelin (APLN) plays regulatory roles in different organs. However, its role in esophageal cancer remains unknown. Therefore, our study aims to explore the effect of APLN on esophageal cancer. One hundred and eighty-four (184) esophageal tumor tissues samples from patients with esophageal cancer, and 11 esophageal tissues samples from healthy volunteers were analyzed for the expression of APLN. APLN was highly expressed in the tumor of patients with esophageal cancer and esophageal cancer cells. Patients with high express...
Source: European Journal of Histochemistry - June 21, 2022 Category: Biomedical Science Authors: Yuhan Wang, Gang Wang, Xiaojun Liu, Dong Yun, Qing Cui, Xiaoting Wu, Wenfeng Lu, Xiwen Yang, Ming Zhang Source Type: research
Lobetyolin suppressed lung cancer in a mouse model by inhibiting epithelial-mesenchymal transition
In conclusion, LBT significantly enhances the efficacy of chemotherapy on lung cancer, and the mechanism may be related to the inhibition of epithelial-mesenchymal transition. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - June 21, 2022 Category: Biomedical Science Authors: Lu Liu, Zhankui Liu, Liu Yang, Xue Wu, Jiaying Zhu, Lili Liu, Yang Liu Source Type: research
Inhibition of HMGB1 suppresses inflammation and catabolism in temporomandibular joint osteoarthritis < em > via < /em > NF- κB signaling pathway
HMGB1 is a highly conserved nuclear protein that is rapidly released into the extracellular environment during infection or tissue damage. In osteoarthritis, HMGB1 acts as a pro-inflammatory cytokine inducing a positive feedback loop for synovial inflammation and cartilage degradation. The aim of this study was to explore the role of HMGB1 in inflammation and catabolism of temporomandibular joint osteoarthritis (TMJOA) and whether inhibition of HMGB1 affects TMJOA. Human synovial fibroblasts were incubated with HMGB1, the expression of pro-inflammatory cytokines and catabolic mediators were measured by Western blot and ELI...
Source: European Journal of Histochemistry - June 21, 2022 Category: Biomedical Science Authors: Yan Yan Li, Ya Ping Feng, Li Liu, Jin Ke, Xing Long Source Type: research
Ferroptosis resistance cooperates with cellular senescence in the overt stage of nonalcoholic fatty liver disease/nonalcoholic steatohepatitis
Cellular senescence and ferroptosis are the two main, fine-tuned processes in tissue damage restraint; however, they can be overactivated in pathologies such as nonalcoholic fatty liver disease/nonalcoholic steatohepatitis (NAFLD/NASH), becoming dangerous stimuli. Senescence is characterized by a decline in cell division and an abnormal release of reactive oxygen species (ROS), and ferroptosis is represented by iron deposition associated with an excessive accumulation of ROS. ROS and cellular stress pathways are also drivers of NAFLD/NASH development. The etiology of NAFLD/NASH lies in poor diets enriched in fat and sugar....
Source: European Journal of Histochemistry - June 21, 2022 Category: Biomedical Science Authors: Antonella Vetuschi, Alfredo Cappariello, Paolo Onori, Eugenio Gaudio, Giovanni Latella, Simona Pompili, Roberta Sferra Source Type: research
