Localization of CGRP and VEGF mRNAs in the mouse superior cervical ganglion during pre- and postnatal development
The neuropeptide calcitonin gene-related peptide (CGRP) mediates inflammation and head pain by influencing the functional vascular blood supply. CGRP is a well-characterized mediator of receptor-regulated neurotransmitter release. However, knowledge regarding the role of CGRP during the development of the superior cervical ganglion (SCG) is limited. In the present study, we observed the localization of CGRP and vascular endothelial growth factor (VEGF-A) mRNAs during prenatal development at embryonic day 14.5 (E14.5), E17.5 and postnatal day 1 (P1) usingin situ hybridization. The antisense probe for CGRP was detected byin ...
Source: European Journal of Histochemistry - November 22, 2018 Category: Biomedical Science Source Type: research
Overexpression of kynurenic acid and 3-hydroxyanthranilic acid after rat traumatic brain injury
Using an immunohistochemical technique, we have studied the distribution of kynuneric acid (KYNA) and 3-hydroxyanthranilic acid (3-HAA) in a rat brain injury model (trauma). The study was carried out inducing a cerebral ablation of the frontal motor cortex. Two mouse monoclonal specific antibodies previously developed by our group directed against KYNA and 3-HAA were used. In control animals (sham-operated), the expression of both KYNA and 3-HAA was not observed. In animals in which the ablation was performed, the highest number of immunoreactive cells containing KYNA or 3-HAA was observed in the region surrounding the les...
Source: European Journal of Histochemistry - November 14, 2018 Category: Biomedical Science Source Type: research
Ultrastructural histochemistry in biomedical research: Alive and kicking
The high-resolution images provided by the electron microscopy has constituted a limitless source of information in any research field of life and materials science since the early Thirties of the last century. Browsing the scientific literature, electron microscopy was especially popular from the 1970 ’s to 80’s, whereas during the 90’s, with the advent of innovative molecular techniques, electron microscopy seemed to be downgraded to a subordinate role, as a merely descriptive technique. Ultrastructural histochemistry was crucial to promote theRenaissance of electron microscopy, when it became evident that a precis...
Source: European Journal of Histochemistry - November 7, 2018 Category: Biomedical Science Source Type: research
Cytoplasmic lattices are not linked to mouse 2-cell embryos developmental arrest
Cytoplasmic lattices are important regulators of oocyte maturation. They store components of the protein synthesis machinery including ribosomes and, among others, they are involved in the regulation of microtubule dynamics in both mouse and human. Cytoplasmic lattices undergo dramatic reorganizations at crucial stages of oocyte maturation, where they are abundantly present in the cytoplasm of developmentally competent oocytes named SN (Surrounded Nucleolus) while they are rare in the cytoplasm of 2-cell stage-arresting NSN (Not Surrounded Nucleolus) oocytes, suggestive of a requirement of cytoplasmic lattices for developm...
Source: European Journal of Histochemistry - October 25, 2018 Category: Biomedical Science Source Type: research
Regenerative potential of the Bichat fat pad determined by the quantification of multilineage differentiating stress enduring cells
Published studies regarding Bichat fat pad focused, quite exclusively, on the implant of this adipose depot for different facial portions reconstruction. The regenerative components of Bichat fat pad were poorly investigated. The present study aimed to describe by an ultrastructural approach the Bichat fat pad, providing novel data at the ultrastructural and cellular level. This data sets improve the knowledge about the usefulness of the Bichat fat pad in regenerative and reconstructive surgery. Bichat fat pads were harvested form eight patients subjected to maxillofacial, dental and aesthetic surgeries. Biopsies were used...
Source: European Journal of Histochemistry - October 23, 2018 Category: Biomedical Science Source Type: research
Mouse oocyte development - Methods and Protocols
The Springer Protocols series “Methods in Molecular Biology” has published its 1818th volume which is entirely devoted to the development of the female gamete: the oocyte. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - September 25, 2018 Category: Biomedical Science Source Type: research
Study of liver in HBV-related hepatocellular carcinoma: Stereology shows quantitative differences in liver structure
Hepatocellular carcinoma is one of the main consequences of liver chronic disease. Hepatocellular carcinoma-related changes may be seen in patients with chronic hepatitis B. The aim of the current study was to quantitate liver tissue elements by stereological technique in patients with hepatitis B-related cancer and compare the results with control and only hepatitis B group. Needle liver biopsies from 40 patients with only chronic hepatitis B infection, from 41 patients with only early hepatocellular carcinoma, from 40 patients with early hepatitis B-related cancer and 30 healthy subjects (control group) were analyzed by ...
Source: European Journal of Histochemistry - September 18, 2018 Category: Biomedical Science Source Type: research
Cancer stem cell markers ALDH1 and CD44+/CD24- phenotype and their prognosis impact in invasive ductal carcinoma
Breast cancer is a very heterogeneous disease. The intrinsic molecular subtypes can explain the intertumoral heterogeneity and the cancer stem cell (CSC) hypothesis can explain the intratumoral heterogeneity of this kind of tumor. CD44+/CD24- phenotype and ALDH1 expression are the major CSC markers described in invasive breast cancer. In the present study, 144 samples of invasive breast carcinoma, no special type were distributed in 15 tissue microarrays (TMA) and then evaluated for expression of the CD44+/CD24- phenotype and ALDH1 to understand the importance of these CSC markers and the clinical aspects of breast cancer....
Source: European Journal of Histochemistry - September 3, 2018 Category: Biomedical Science Source Type: research
Low ozone concentrations promote adipogenesis in human adipose-derived adult stem cells
In this study, we investigated the effects of mild ozonisation on human adipose-derived adult stem (hADAS) cells i.e., mesenchymal stem cells occurring in the stromal-vascular fraction of the fat tissue and involved in the tissue regeneration processes. hADAS cells were induced to differentiate into the adipoblastic lineage, and the effect of low ozone concentrations on the adipogenic process was studied by combining histochemical, morphometric and ultrastructural analyses. Our results demonstrate that ozone treatment promotes lipid accumulation in hADAS without inducing deleterious effects, thus paving the way to future s...
Source: European Journal of Histochemistry - September 3, 2018 Category: Biomedical Science Source Type: research
From fix to fit into the autoptic human brains
In this study, we analyzed four types of fixation systems and compared histo and immuno-staining. Based on our results, we propose a modified method of combined fixation in formalin and formic acid for the autoptic adult brain to obtain easy, fast, safe and efficient immunolabelling of long-stored FFPE tissue. In particular, we have achieved an improved preservation of cellular morphology and obtained success in postmortem immunostaining for NeuN. This nuclear antigen is an important marker for mapping neurons, for example, to evaluate the histopathology of temporal lobe epilepsy or to draw the topography of cardiorespirat...
Source: European Journal of Histochemistry - August 27, 2018 Category: Biomedical Science Source Type: research
Analysis of mineral apposition rates during alveolar bone regeneration over three weeks following transfer of BMP-2/7 gene via in vivo electroporation
Alveolar bone is not spontaneously regenerated following trauma or periodontitis. We previously proposed an animal model for new alveolar bone regeneration therapy based on the non-viral BMP-2/7 gene expression vector andin vivo electroporation, which induced the formation of new alveolar bone over the course of a week. Here, we analysed alveolar bone during a period of three weeks following gene transfer to periodontal tissue. Non-viral plasmid vector pCAGGS-BMP-2/7 or pCAGGS control was injected into palatal periodontal tissue of the first molar of the rat maxilla and immediately electroporated with 32 pulses of 50 V for...
Source: European Journal of Histochemistry - August 9, 2018 Category: Biomedical Science Source Type: research
Alveolar bone regeneration during three weeks following transfer of BMP-2/7 gene via in vivo electroporation
Alveolar bone is not spontaneously regenerated following trauma or periodontitis. We previously proposed an animal model for new alveolar bone regeneration therapy based on the non-viral BMP-2/7 gene expression vector andin vivo electroporation, which induced the formation of new alveolar bone over the course of a week. Here, we analysed alveolar bone during a period of three weeks following gene transfer to periodontal tissue. Non-viral plasmid vector pCAGGS-BMP-2/7 or pCAGGS control was injected into palatal periodontal tissue of the first molar of the rat maxilla and immediately electroporated with 32 pulses of 50 V for...
Source: European Journal of Histochemistry - August 9, 2018 Category: Biomedical Science Source Type: research
Expression of acetylated tubulin in the postnatal developing mouse cochlea
Acetylation tubulin is one of the major post-translational modifications of microtubules. Stable microtubules are well known to contain acetylated tubulin. Here, we examined the spatiotemporal expression of acetylated tubulin in the mouse cochlea during postnatal development. At postnatal day 1 (P1), acetylated tubulin was localized primarily to the auditory nerve inside the cochlea and their synaptic contacts with the inner and outer hair cells (IHCs and OHCs). In the organ of Corti, acetylated tubulin occurred first at the apex of pillar cells. At P5, acetylated tubulin first appeared in the phalangeal processes of Deite...
Source: European Journal of Histochemistry - August 8, 2018 Category: Biomedical Science Source Type: research
Interaction between sphingosine kinase/sphingosine 1 phosphate and transforming growth factor- β/Smads pathways in experimental intestinal fibrosis. An in vivo immunohistochemical study
This study highlights the relationship between the two pathways and the possible role of SPHK1 in the intestinal fibrosis. These results, if confirmed byin vitro studies, may have important clinical implications in the development of new therapeutical approaches in inflammatory bowel disease. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - July 31, 2018 Category: Biomedical Science Source Type: research
Effect of high-fat mixed lipid diet and swimming on fibre types in skeletal muscles of rats with colon tumours
Skeletal muscle fibre types, whose characteristics are determined by myosin heavy chain (MyHC) isoforms, can adapt to changed physiological demands with changed MyHC isoform expression resulting in the fibre type transitions. The endurance training is known to induce fast-to-slow transitions and has beneficial effect in carcinogenesis, whereas the effect of an excessive fat intake and its interaction with the effect of swimming are less conclusive. Therefore, we studied the effect of high-fat mixed lipid (HFML) diet and long-term (21-week) swimming on fibre type transitions and their average diameters by immunohistochemica...
Source: European Journal of Histochemistry - July 25, 2018 Category: Biomedical Science Source Type: research
