In memoriam of Prof. Antonio Lauria
(Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - September 28, 2020 Category: Biomedical Science Authors: Editor in Chief Source Type: research

Stem Cells and Therapy: Emerging Approaches
The ten chapters of this volume exploit the potentials of stem cells biology in translational medicine in a very clear way also thanks to nice color illustrations together with tricks and suggestions useful to get reproducible results... (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - September 11, 2020 Category: Biomedical Science Authors: Manuela Monti Source Type: research

An improved method for preparation of mouse retinal cryosections
Immunohistochemistry using mouse retinal cryosections is a routine assay used in vision research. However, retinal tissues are fragile, and it is difficult to obtain an ideal retinal cryosection. Here, we developed a modified method for preparing retinal cryosection. Super Glue was applied on the surface of the sclera before the cornea and the lens are removed from either the unfixed or PFA-fixed mouse eyeballs. The new methods largely prevented retinal detachment in mouse retinal cryosections. Immunostaining of retinal cryosections derived from PFA-fixed mouse eyes using rod and cone markers yielded high-quality...
Source: European Journal of Histochemistry - September 3, 2020 Category: Biomedical Science Authors: Ling Li, Yongqiong Lin, Fang Yang, Tongdan Zou, Jialiang Yang, Houbin Zhang Source Type: research

Effect of TNBS-induced colitis on enteric neuronal subpopulations in adult zebrafish
This study focuses on the effect of 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis on changes in enteric neuronal subpopulations in adult zebrafish. These changes are suggested to be related to the altered neuro-immune interactions and GI motility, and in IBD pathogenesis. New insights into neuroplasticity will be instrumental in finding appropriate therapeutic treatments. TNBS was intraluminally administered in the distal intestine (DI) of anesthetized adult zebrafish. A histological time course of the intestinal inflammatory response was created to establish optimal TNBS concentration and acute inflammation ...
Source: European Journal of Histochemistry - August 28, 2020 Category: Biomedical Science Authors: Leen Uyttebroek, Casper Pype, Guy Hubens, Jean-Pierre Timmermans, Luc van Nassauw Source Type: research

Effect of carboplatin injection on Bcl-2 protein expression and apoptosis induction in Raji cells
To investigate the effects of carboplatin (CBP) injection on apoptosis induction in the human lymphoma cell line Raji and to explore the underlying mechanism, Raji cells were randomly divided into two treatment groups. Cells in the experimental groups were treated with 15 μM CBP injection, those in the control groups were treated with solvent, and both groups were treated for 24, 48 and 72 h. Cells from each group were collected for subsequent assays. For each group, the relative expression of B-cell lymphoma-2 (Bcl-2) was determined by Western blot (WB), the expres sion pattern of Bcl-2 was observed by immunocytochemis...
Source: European Journal of Histochemistry - July 1, 2020 Category: Biomedical Science Authors: Peng Lin, Boliang Zhou, Haiying Yao, Ya-ping Guo Source Type: research

Imaging techniques in nanomedical research
About twenty years ago, nanotechnology began to be applied to biomedical issues giving rise to the research field called nanomedicine. Thus, the study of the interactions between nanomaterials and the biological environment became of primary importance in order to design safe and effective nanoconstructs suitable for diagnostic and/or therapeutic purposes. Consequently, imaging techniques have increasingly been used in the production, characterisation and preclinical/clinical application of nanomedical tools. This work aims at making an overview of the microscopy and imaging techniquesin vivo andin vitro in their applicati...
Source: European Journal of Histochemistry - July 1, 2020 Category: Biomedical Science Authors: Laura Calderan, Manuela Malatesta Source Type: research

Dark-field microscopy enhance visibility of CD31 endothelial staining
A simple dark field microscopy technique was used for visualization of blood vessels in normal human renal tissues and carcinoma. Phase contrast condenser ring apt for high power objectives was combined with a 10x objective in order to create a dark field illumination of the specimens examined. The endothelial lining of the vessels had been stained by using CD31 monoclonal antibodies combined with conventional peroxidase immunohistochemistry. The final DAB addition used for this technique induced an intense light scatter in the dark field microscope. This scattered light originating from the endothelial lining made the wal...
Source: European Journal of Histochemistry - July 1, 2020 Category: Biomedical Science Authors: Eva Jennische, Stefan Lange, Ragnar Hultborn Source Type: research

The synovial surface of the articular cartilage
The articular cartilage has been the subject of a huge amount of research carried out with a wide array of different techniques. Most of the existing morphological and ultrastructural data on the this tissue, however, were obtained either by light microscopy or by transmission electron microscopy. Both techniques rely on thin sections and neither allows a direct, face-on visualization of the free cartilage surface (synovial surface), which is the only portion subject to frictional as well as compressive forces. In the present research, high resolution visualization by scanning electron microscopy and by atomic force micros...
Source: European Journal of Histochemistry - July 1, 2020 Category: Biomedical Science Authors: Petra Rita Basso, Elena Carava ' , Marina Protasoni, Marcella Reguzzoni, Mario Raspanti Source Type: research

Combined lectin- and immuno-histochemistry (CLIH) for applications in cell biology and cancer diagnosis: Analysis of human urothelial carcinomas
Lectin histochemistry (LHC) and immunohistochemistry (IHC), which demonstrate the composition and localisation of sugar residues and proteins in cell membranes, respectively, are generally used separately. Using these two methods, we previously demonstrated that malignant transformation of urothelial cells results in the alterations of protein glycosylation and reduced expression of urothelium-specific integral membrane proteins uroplakins (UPs). However, the correlation between these changes was not studied yet. To evaluate this correlation, we developed innovative method, which we named combined lectin- and immuno- histo...
Source: European Journal of Histochemistry - July 1, 2020 Category: Biomedical Science Authors: Da ša Zupančič, Mateja Erdani Kreft, Igor Sterle, Rok Romih Source Type: research

A morphological analysis of fresh and brine-cured olives attacked by < em > Bactrocera oleae < /em > using light microscopy and ESEM-EDS
The present study investigated the morphology of fresh and brine-cured table olives (TOs) as well as the changes that occur when drupes are attacked by the fruit flyBactrocera oleae. Morphological analyses were performed using light microscopy (LM) and environmental scanning electron microscopy coupled with energy dispersive spectroscopy (ESEM-EDS). The LM analysis was carried out with visible light to evaluate sections stained with either PAS or Azan mixtures as well as unstained sections observed at fluorescence microscopy. The results of the analyses showed that: i) Azan and PAS staining played a useful complementary ro...
Source: European Journal of Histochemistry - July 1, 2020 Category: Biomedical Science Authors: Barbara Lanza, Anna Panato, Laura Valentini, Pamela Rodegher, Federica Bortolotti, Michela Battistelli, Paolino Ninfali, Pietro Gobbi Source Type: research

β-catenin mediates the effect of GLP-1 receptor agonist on ameliorating hepatic steatosis induced by high fructose diet
The hypoglycemic drug GLP-1 receptor agonist can ameliorate hepatic steatosis but the mechanism is not clear. Intake of high fructose leads to non-alcoholic fatty liver disease by stimulating lipid synthesis, and β-catenin is the key molecule for realizing GLP-1 function in extrahepatic tissues; with the discovery of GLP-1 receptor in liver, we speculate that β-catenin might mediate GLP-1 receptor agonist on ameliorating hepatic steatosis induced by high fructose. Wistar rats were fed with high fructose di et for 8 weeks and then treated with GLP-1 receptor agonist exenatide for 4 weeks; the changes of lipid synt...
Source: European Journal of Histochemistry - July 1, 2020 Category: Biomedical Science Authors: Zhe Gao, Guang-Yao Song, Lu-Ping Ren, Hui-Juan Ma, Bo-Qing Ma, Shu-Chun Chen Source Type: research

Morphological and ultrastructural characteristics of the tongue of wild boar
In conclusion, we found new descriptions and shapes of the CTCs of the lingual papillae. In addition, we demonstrated the epithelium structural characteristics, the nuclear distribution between the epithelial layers, and the ultrastructural aspects of the dorsal epithelium of the tongue. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - May 5, 2020 Category: Biomedical Science Authors: Gabriela de Souza Reginato, Gabriela Klein Barbosa, Amanda Olivotti Ferreira, Bruno Gomes Vasconcelos, Rose Eli Grassi Rici, Ii-sei Watanabe, Adriano Polican Ciena Source Type: research

Defense and protection mechanisms in lung exposed to asbestiform fiber: the role of macrophage migration inhibitory factor and heme oxygenase-1
Fluoro-edenite (FE), an asbestiform fiber, is responsible for many respiratory pathologies: chronic obstructive diseases, pleural plaques, fibrosis, and malignant mesothelioma. Macrophage migration inhibitory factor (MIF) is one of the first cytokines produced in response to lung tissue damage. Heme oxygenase-1 (HO-1) is a protein with protective effects against oxidative stress. It is up regulated by several stimuli including pro-inflammatory cytokines and factors that promote oxidative stress. In this research, thein vivo model of sheep lungs naturally exposed to FE was studied in order to shed light on the pathophysiolo...
Source: European Journal of Histochemistry - April 16, 2020 Category: Biomedical Science Source Type: research

MMP-7 and MMP-9 are overexpressed in the synovial tissue from severe temporomandibular joint dysfunction
The objective of this study was to determine the relation of MMP-7 and MMP-9 expression with severe temporomandibular joint dysfunction, in particular with anterior disk displacement without reduction (ADDwoR), using an immunohistochemical approach. Therefore, twenty human temporomandibular synovia in the test group and ten in the control group were collected. The results showed there was a statistically significant difference (P
Source: European Journal of Histochemistry - April 16, 2020 Category: Biomedical Science Source Type: research

Ozone at low concentrations does not affect motility and proliferation of cancer cells in vitro
Exposure to low ozone concentrations is used in medicine as an adjuvant/complementary treatment for a variety of diseases. The therapeutic potential of low ozone concentrations relies on their capability to increase the nuclear translocation of the Nuclear factor erythroid 2-related factor 2 (Nrf2), thus inducing the transcription of Antioxidant Response Elements (ARE)-driven genes and, through a cascade of events, a general cytoprotective response. However, based on the controversial role of Nrf2 in cancer initiation, progression and resistance to therapies, possible negative effects of ozone therapy may be hypothesised i...
Source: European Journal of Histochemistry - April 2, 2020 Category: Biomedical Science Source Type: research

Isolation of rat hepatocytes for pharmacological studies on metabotropic glutamate receptor (mGluR) subtype 5: a comparison between collagenase I versus collagenase IV
Isolated hepatocytes can be obtained from the liver by collagenase infusion, a procedure that could affect cell isolation as well as the integrity of membrane receptors. In this respect we compared metabotropic glutamate subtype 5 receptor (mGluR5) protein expression and activity in rat hepatocytes isolated by two collagenases, type I and type IV. Hepatocytes were isolated from male Wistar rats (200-250 g) using collagenase I or collagenase IV and after isolation, viability and morphology of rat hepatocytes were assessed measuring mGluR5 protein expression by Western blot analyses. mGluR5 activation was evaluated by inosit...
Source: European Journal of Histochemistry - March 26, 2020 Category: Biomedical Science Source Type: research

A new method for the direct tracking of < em > in vivo < /em > lignin nanocapsules in < em > Eragrostis tef < /em > (Poaceae) tissues
Environmental concerns have driven scientists to research new eco-friendly approaches for the preparation of nanosystems. For this purpose, novel bio-polymers have been selected. Among these, one of the most promising is lignin, which is biodegradable and biocompatible. Additionally, lignin is one of the main by-products of the paper industry and can be re-used in nanosystems building. Lignin-based nanosystems could be used in agriculture, to improve the uptake of bioactive compounds, thus avoiding soil pollution. However, the mechanism of penetration in the plant and the route of transportation within the internal plant t...
Source: European Journal of Histochemistry - March 26, 2020 Category: Biomedical Science Source Type: research

A new method for the direct tracking of < em > in vivo < /em > lignin nanocapsules in Eragrostis tef (Poaceae) tissues
Environmental concerns have driven scientists to research new eco-friendly approaches for the preparation of nanosystems. For this purpose, novel bio-polymers have been selected. Among these, one of the most promising is lignin, which is biodegradable and biocompatible. Additionally, lignin is one of the main by-products of the paper industry and can be re-used in nanosystems building. Lignin-based nanosystems could be used in agriculture, to improve the uptake of bioactive compounds, thus avoiding soil pollution. However, the mechanism of penetration in the plant and the route of transportation within the internal plant t...
Source: European Journal of Histochemistry - March 26, 2020 Category: Biomedical Science Source Type: research

A new method for the direct tracking of in vivo lignin nanocapsules in Eragrostis tef (Poaceae) tissues
Environmental concerns have driven scientists to research new eco-friendly approaches for the preparation of nanosystems. For this purpose, novel bio-polymers have been selected. Among these, one of the most promising is lignin, which is biodegradable and biocompatible. Additionally, lignin is one of the main by-products of the paper industry and can be re-used in nanosystems building. Lignin-based nanosystems could be used in agriculture, to improve the uptake of bioactive compounds, thus avoiding soil pollution. However, the mechanism of penetration in the plant and the route of transportation within the internal plant t...
Source: European Journal of Histochemistry - March 26, 2020 Category: Biomedical Science Source Type: research

Presence of N-acetylneuraminic acid in the lung during postnatal development
Sialic acids, particularly N-acetylneuraminic acid (Neu5Ac), are present as terminal components of rich and complex oligosaccharide chains, which are termed glycans, and are exhibited on the cell surfaces, especially on epithelial cells. Crucial in the ‘social behavior’ of the cell, sialic acids play vital roles in many physiological and pathological phenomena. The aim of the present study was to separate, identify, and quantify Neu5Ac in purified lung membranes from 4-, 14-, and 21-day-old animals, followed by the statistical analysis of thes e results with our previously reported data (0-day-old and adult res...
Source: European Journal of Histochemistry - March 26, 2020 Category: Biomedical Science Authors: Maria de F átima Martins, Marco S. Freitas, Ana Honório-Ferreira, Carlos Alberto Gonçalves Source Type: research

The substance P and neurokinin-1 receptor system in human thyroid cancer: an immunohistochemical study
This study aims to examine by immunohistochemistry, the localization of SP and the NK-1R in human TC samples (papillary, follicular, medullary, anaplastic), in metastasis and in healthy thyroid samples. SP and the NK-1R were expressed in all normal and TC samples. In healthy glands, SP was located in follicular cells (nucleus) and colloid and NK-1R in follicular cells (cytoplasm) and stroma. In TC samples, SP was visualized in follicular cells (nucleus and cytoplasm), stroma and colloid and NK-1R in follicular cells (cytoplasm), stroma and colloid. A semiquantitative scoring system (Allred Unit Scoring System) was applied....
Source: European Journal of Histochemistry - March 26, 2020 Category: Biomedical Science Authors: Inmaculada Isorna, Francisco Esteban, Juan Solanellas, Rafael Cove ñas, Miguel Muñoz Source Type: research

Seasonal expressions of SPAG11A and androgen receptor in the epididymis of the wild ground squirrels ( < em > Citellus dauricus < /em > Brandt)
Sperm-associated antigen 11A (SPAG11A) is expressed exclusively in the epididymis, which can specifically regulate sperm maturation. The aim of this study was to investigate the seasonal expressions of beta-defensin (SPAG11A) and androgen receptor (AR) in the epididymis of the wild ground squirrels (Citellus dauricusBrandt). Morphologically, the results showed that epididymis length and weight in the breeding season were significantly higher than those of the non-breeding season. Histologically, the results revealed that enlarged lumen diameters, thickened epithelial and abundant sperm in the breeding season while reduced ...
Source: European Journal of Histochemistry - March 26, 2020 Category: Biomedical Science Source Type: research

The psoriatic shift induced by interleukin 17 is promptly reverted by a specific anti-IL-17A agent in a three-dimensional organotypic model of normal human skin culture
Interleukin 17A (IL-17A), mainly produced by the T helper subclass Th17, plays a key role in the psoriatic plaque formation and progression. The clinical effectiveness of anti-IL-17A agents is documented, but the early and specific mechanisms of their protection are not identified yet. The challenge of the present study is to investigate the possible reversal exerted by a specific anti-IL-17A agent on the psoriatic events induced by IL-17A in a three-dimensional organotypic model of normal human skin. Bioptic skin fragments obtained after aesthetic surgery of healthy women (n=5) were incubated with i) IL-17A biological inh...
Source: European Journal of Histochemistry - March 26, 2020 Category: Biomedical Science Source Type: research

Thrombin regulates the ability of Schwann cells to support neuritogenesis and to maintain the integrity of the nodes of Ranvier
Schwann cells (SC) are characterized by a remarkable plasticity that enables them to promptly respond to nerve injury promoting axonal regeneration. In peripheral nerves after damage SC convert to a repair-promoting phenotype activating a sequence of supportive functions that drive myelin clearance, prevent neuronal death, and help axon growth and guidance. Regeneration of peripheral nerves after damage correlates inversely with thrombin levels. Thrombin is not only the key regulator of the coagulation cascade but also a protease with hormone-like activities that affects various cells of the central and peripheral nervous ...
Source: European Journal of Histochemistry - March 26, 2020 Category: Biomedical Science Source Type: research

Expression of E-cadherin, Ki-67, and p53 in urinary bladder cancer in relation to progression, survival, and recurrence
In this study, we analyzed the tumor tissues of 224 patients with pTa, pT1, and pT2 urinary bladder cancer. We performed a histomorphologic analysis and immunohistochemistry for p53, Ki-67, and E-cadherin, which were selected as markers of the malignant process. For pTa and pT1, univariate analyses of cancer-specific survival (CSS), progression-free survival (PFS), and recurrence-free survival (RFS) were calculated using the Kaplan-Meier method, the log-rank test and Cox regression. Multivariate analysis was performed by a Cox regression analysis. Ki-67 (P
Source: European Journal of Histochemistry - March 26, 2020 Category: Biomedical Science Source Type: research

Erratum: Correction of Affiliation
This corrects the article " Anastomotic healing in a rat model of peritonitis after non-steroidal anti-inflammatory drug administration " in volume 64(1):3085In the published article “Anastomotic healing in a rat model of peritonitis after non-steroidal anti-inflammatory drug administration” Eur J Histochem 2020;64(1):3085,https://doi.org/10.4081/ejh.2020.3085, ” one affiliation was published incorrectly. The authors apologize for any inconvenience that it may have caused.Roberto Ghiselli,1 Guendalina Lucarini,2 Monica Ortenzi,1 Eleonora Salvolini,3 Stefania Saccomanno,2Fiorenza Orlando,4 Mauro ...
Source: European Journal of Histochemistry - February 26, 2020 Category: Biomedical Science Source Type: research

Chronic cypermethrin exposure alters mouse embryonic stem cell growth kinetics, induces Phase II detoxification response and affects pluripotency and differentiation gene expression
Worldwide uncontrolled use of synthetic pyrethroids contaminates water and soil leading to health hazards. Cypermethrin (CYP), the most used pyrethroid, induces detrimental effects on adults and embryos at different stages of development of several vertebrate species. In Mammals, CYP-induced alterations have been previously described in adult somatic cells and in post-implantation embryos. It remains unknown whether CYP has effects during pre-implantation development. Studies to access pre-implantation embryo toxicity are complicated by the restricted number of blastocysts that may be obtained, eitherin vivo orin vitro. Em...
Source: European Journal of Histochemistry - February 17, 2020 Category: Biomedical Science Source Type: research

Immunolocalization of aromatase P450 in the epididymis of Podarcis sicula and Rattus rattus
The goal of this study was to evaluate P450 aromatase localization in the epididymis of two different vertebrates: the lizardPodarcis sicula, a seasonal breeder, andRattus rattus, a continuous breeder. P450 aromatase is a key enzyme involved in the local control of spermatogenesis and steroidogenesis and we proved for the first time that this enzyme is represented in the epididymis of bothP. sicula andR. rattus. In details, P450 aromatase was well represented in epithelial and myoid cells and in the connective tissue ofP. sicula epididymis during the reproductive period; instead, during autumnal resumption this enzyme was ...
Source: European Journal of Histochemistry - January 27, 2020 Category: Biomedical Science Source Type: research

Can the AGE/RAGE/ERK signalling pathway and the epithelial-to-mesenchymal transition interact in the pathogenesis of chronic rhinosinusitis with nasal polyps?
Chronic rhinosinusitis with nasal polyps (CRSwNP) is a persistent sinonasal mucosa inflammatory disease with still unclear pathophysiologic mechanisms that imply events of tissue repair and structural remodelling. Several cascades seem to have a considerable role in the onset and progression of mucosa hyperproliferation in nasal polyps including transforming growth factor β/Small mother against decapentaplegic (TGFβ/Smads), mitogenactivated protein kinases (MAPKs), advanced glycosylation end-products (AGEs) together with epithelial-tomesenchymal transition (EMT). Since many inflammatory mediators are reported to ...
Source: European Journal of Histochemistry - January 23, 2020 Category: Biomedical Science Source Type: research

Nuclear factor-kB and nitric oxide synthases in red blood cells: good or bad in obesity? A preliminary study
In conclusion, we have considered the literature that focuses on the omega-6/omega-3 ratio. The ratio changed from the past, especially in people whose diet is strongly westernized worsening the state of health of the patient and leading to an higher incidence of obesity. Our study hypoth esizes that the supplementation could help to restore the correct ratio. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - January 21, 2020 Category: Biomedical Science Source Type: research

Immuno- and glyco-histochemistry as a tool to evaluate the oregano supplemented feed effects in pig gut
This study evaluated the effects of adding an aqueous extract of oregano (OAE) to the diet of two homogenous groups of pigs during the finisher phase. The diets were as follows: control commercial diet (CTR group) and CTR diet supplemented (2 g/kg) with OAE (O group). Samples of ileum and caecum from the two groups were examined by conventional histochemistry to analyze complex carbohydrates and by immunohistochemistry to detect Bcl-2 Associate X protein (BAX), an indicator of oxidative stress. Glyco-histochemistry showed significant differences between the two groups. Immunohistochemistry revealed a lower presence of BAX ...
Source: European Journal of Histochemistry - January 10, 2020 Category: Biomedical Science Source Type: research

Expression, localization and synthesis of small leucine-rich proteoglycans in developing mouse molar tooth germ
The gene expression and protein synthesis of small leucine-rich proteoglycans (SLRPs), including decorin, biglycan, fibromodulin, and lumican, was analyzed in the context of the hypothesis that they are closely related to tooth formation.In situ hybridization, immunohistochemistry, and organ culture with metabolic labeling of [35S] were carried out in mouse first molar tooth germs of different developmental stages using ICR mice at embryonic day (E) 13.5 to postnatal day (P) 7.0. At the bud and cap stage,decorin mRNA was expressed only in the surrounding mesenchyme, but not within the tooth germ.BiglycanmRNA was then expre...
Source: European Journal of Histochemistry - January 10, 2020 Category: Biomedical Science Source Type: research

Anastomotic healing in a rat model of peritonitis after non-steroidal anti-inflammatory drug administration
The tissue inflammatory response can influence the outcome of anastomotic healing. Anastomotic leakage represents a dreadful complication after gastrointestinal surgery, in particular sepsis and intra-abdominal infections impair the restorative process of colic anastomoses. It has been debated whether the administration of non-steroidal anti-inflammatory drugs (NSAIDs) is a risk factor for dehiscence, since many patients receive NSAIDs in the early postoperative period. Our aim was, for the first time, to analyze the morpho-functional effects of postoperative administration of two commonly used NSAIDs, Diclofenac and Ketor...
Source: European Journal of Histochemistry - January 10, 2020 Category: Biomedical Science Source Type: research

Morphological analysis of the seeds of three pseudocereals by using light microscopy and ESEM-EDS
The seed morphology of three Pseudocereal Grains (PSCg),i.e. quinoa (Chenopodium quinoaWilld,Chenopodiaceae), buckwheat (Fagopyrum esculentumMoench,Polygonaceae) and amaranth (Amaranthus caudatusL.,Amaranthaceae) was studied by light microscopy (LM) and Environmental Scanning Electron Microscopy coupled with Energy Dispersive Spectroscopy (ESEM-EDS). LM was used with visible light to evaluate either unstained sections or sections stained with Azan mixture and with fluorescent light. The aim of the study was to compare the architecture of the three seeds in order to connect their morphology with nutrient localization. The A...
Source: European Journal of Histochemistry - January 10, 2020 Category: Biomedical Science Source Type: research

Immunohistochemical study for relationship between vessel and lymphatic properties and tooth marks in human oral mucosa
Angiogenesis is an important issue related to normal growth and differentiation, and it is a critical issue in the progression of human disease in oral mucosa. Tooth marks occur after clenching the teeth for a long period under muscle tension in the human oral cavity. However, the sites of angiogenesis, cell differentiation and microvessel density are not known for human mucosa with tooth marks. Therefore, we investigated the relationship between the markers of differentiation (Ki-67), angiogenesis (CD31, D2-40, VEGF-A), and marks from teeth in the second molar region using immunohistochemical methods. In addition, we comp...
Source: European Journal of Histochemistry - January 10, 2020 Category: Biomedical Science Source Type: research

A journal of histochemistry as a forum for non-histochemical scientific societies
Histochemical techniques are widely applied in biomedical research and, during the last twenty years, they were among the methods used in more than 590,000 scientific articles in indexed journals. However, a very small percentage of these papers were published in strictly histochemical journals. A possible strategy to widen the audience of the histochemical journals making them attractive to non-histochemist authors might be to publish and make open-access available the proceedings of the meetings and conferences of valued scientific societies whose fellows use microscopy and histochemistry in their experimental activity. ...
Source: European Journal of Histochemistry - December 23, 2019 Category: Biomedical Science Source Type: research

Alcian blue staining to track the intracellular fate of hyaluronic-acid-based nanoparticles at transmission electron microscopy
In this study, the critical-electrolyte-concentration Alcian Blue method set up by Schofieldet al. in 1975 was applied to track hyaluronic-acid-based NPs in muscle cellsin vitro. This long-established histochemical method proved to be a powerful tool allowing to identify not only whole NPs while entering cells and moving into the cytoplasm, but also their remnants following lysosomal degradation and extrusion. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - December 12, 2019 Category: Biomedical Science Source Type: research

Downregulation of SRPK2 promotes cell cycle arrest though E2F1 in non-small cell lung cancer
Serine-arginine protein kinase (SRPK) belongs to a class of cell cycle regulating kinases that can phosphorylate proteins containing serine/arginine-Rich (SR) regions. SR proteins are a family of RNA binding phosphoproteins that control both constitutive and alternative pre-mRNA splicing events. However, little is known about their role in non-small cell lung cancer (NSCLC). In the present study, we found that serine-arginine protein kinase 2 (SRPK2) expression was upregulated in NSCLC tissues compared with adjacent normal tissues. Kaplan-Meier curve analyses showed that the overall survival time of NSCLC patients with hig...
Source: European Journal of Histochemistry - December 11, 2019 Category: Biomedical Science Source Type: research

Bleaching melanin in formalin-fixed and paraffin-embedded melanoma specimens using visible light: a pilot study
In conclusion, visible light can be effectively exploited to bleach pigmented melanoma FFPE sections prior to perform routine histochemical and immunohistochemical stains. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - October 31, 2019 Category: Biomedical Science Source Type: research

Increased expression of STAT3 and SOCS3 in placenta from hyperglycemic rats
Signal transducer and activator of transcription 3 (STAT3) is a transcription factor that is activated by interleukin (IL)-6 and IL-10 that generate nearly opposing responses. The suppressor of cytokine signaling 3 (SOCS3) is the negative regulator of STAT3 and plays an important role in the negative regulation of the inflammatory process. Evidence has shown the importance of STAT3 and SOCS3 during implantation and normal pregnancy. However, little is known about the relationship of both factors under hyperglycemic condition. The aim of this study was to evaluate the placenta regions exhibiting immunopositivity for STAT3 a...
Source: European Journal of Histochemistry - October 30, 2019 Category: Biomedical Science Source Type: research

Erythrocytes of the Rhesus and Cynomolgus Monkeys
The monographErythrocytes of the Rhesus and Cynomolgus Monkeys is devoted to the cytologic, quantitative, generative normal and abnormal presentations of the erythrocytes in these two species of primates... (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - October 22, 2019 Category: Biomedical Science Source Type: research

Bradykinin and noradrenaline preconditioning influences level of antioxidant enzymes SOD, CuZn-SOD, Mn-SOD and catalase in the white matter of spinal cord in rabbits after ischemia/reperfusion
The aim of present work is to assess the effects of bradykinin (Br) or noradrenaline (Nor) preconditioning to the levels of antioxidant enzymes: superoxide dismutase (SOD), copper, zinc superoxide dismutase (CuZn-SOD), manganese superoxide dismutase (Mn-SOD) and catalase in ischemia/reperfusion (I/R) model in the rabbit spinal cord white matter as well as effect on glial fibrillary acidic protein (GFAP) and ubiquitin immunoreaction in glial cells. Rabbits were preconditioned by intraperitoneal single dose of Br or Nor 48 h prior to 20 min of ischemia followed by 24 or 48 h of reperfusion. White matter of L3-L6 spinal cord ...
Source: European Journal of Histochemistry - October 18, 2019 Category: Biomedical Science Source Type: research

Immunofluorescence characterization of innervation and nerve-immune cell interactions in mouse lymph nodes
The peripheral nervous system communicates specifically with the immune system via local interactions. These interactions include the “hardwiring” of sympathetic/parasympathetic (efferent) and sensory nerves (afferent) to primary (e.g., thymus and bone marrow) and secondary (e.g., lymph node, spleen, and gut-associated lymphoid tissue) lymphoid tissue/organs. To gain a better understanding of this bidirectional interaction/crosstalk between the two systems, we have investigated the distribution of nerve fibres and PNS-immune cell associationsin situ in the mouse lymph node by using immunofluorescent staining an...
Source: European Journal of Histochemistry - October 18, 2019 Category: Biomedical Science Source Type: research

Digital PCR - Methods and Protocols
Sensitivity, reproducibility, precision and accuracy are recurring words in this book. Indeed, digital PCR (dPCR) represents a major step forward in the quantification of nucleic acids. In this field, for many years it has been used the quantitative PCR (qPCR), a technology that allows a real-time monitoring of DNA amplification which has proved useful for obtaining a relative measurement, but that only indirectly, through the use of reference standards, provides an absolute quantification.... (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - October 18, 2019 Category: Biomedical Science Source Type: research

Bradykinin and noradrenaline preconditioning influences level of antioxidant enzymes SOD, CuZn-SOD, Mn-SOD and catalase in the white matter of spinal cord in rabbits after ischemia/reperfusion
The aim of present work is to assess the effects of bradykinin (Br) or noradrenaline (Nor) preconditioning to the levels of antioxidant enzymes: superoxide dismutase (SOD), copper, zinc superoxide dismutase (CuZn-SOD), manganese superoxide dismutase (Mn-SOD) and catalase in ischemia/reperfusion (I/R) model in the rabbit spinal cord white matter as well as effect on glial fibrillary acidic protein (GFAP) and ubiquitin immunoreaction in glial cells. Rabbits were preconditioned by intraperitoneal single dose of Br or Nor 48 h prior to 20 min of ischemia followed by 24 or 48 h of reperfusion. White matter of L3-L6 spinal cord ...
Source: European Journal of Histochemistry - October 18, 2019 Category: Biomedical Science Source Type: research

Immunofluorescence characterization of innervation and nerve-immune cell interactions in mouse lymph nodes
The peripheral nervous system communicates specifically with the immune system via local interactions. These interactions include the “hardwiring” of sympathetic/parasympathetic (efferent) and sensory nerves (afferent) to primary (e.g., thymus and bone marrow) and secondary (e.g., lymph node, spleen, and gut-associated lymphoid tissue) lymphoid tissue/organs. To gain a better understanding of this bidirectional interaction/crosstalk between the two systems, we have investigated the distribution of nerve fibres and PNS-immune cell associationsin situ in the mouse lymph node by using immunofluorescent staining an...
Source: European Journal of Histochemistry - October 18, 2019 Category: Biomedical Science Source Type: research

Digital PCR - Methods and Protocols
Sensitivity, reproducibility, precision and accuracy are recurring words in this book. Indeed, digital PCR (dPCR) represents a major step forward in the quantification of nucleic acids. In this field, for many years it has been used the quantitative PCR (qPCR), a technology that allows a real-time monitoring of DNA amplification which has proved useful for obtaining a relative measurement, but that only indirectly, through the use of reference standards, provides an absolute quantification.... (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - October 18, 2019 Category: Biomedical Science Source Type: research