Ferroptosis resistance cooperates with cellular senescence in the overt stage of nonalcoholic fatty liver disease/nonalcoholic steatohepatitis
Cellular senescence and ferroptosis are the two main, fine-tuned processes in tissue damage restraint; however, they can be overactivated in pathologies such as nonalcoholic fatty liver disease/nonalcoholic steatohepatitis (NAFLD/NASH), becoming dangerous stimuli. Senescence is characterized by a decline in cell division and an abnormal release of reactive oxygen species (ROS), and ferroptosis is represented by iron deposition associated with an excessive accumulation of ROS. ROS and cellular stress pathways are also drivers of NAFLD/NASH development. The etiology of NAFLD/NASH lies in poor diets enriched in fat and sugar....
Source: European Journal of Histochemistry - June 21, 2022 Category: Biomedical Science Authors: Antonella Vetuschi, Alfredo Cappariello, Paolo Onori, Eugenio Gaudio, Giovanni Latella, Simona Pompili, Roberta Sferra Source Type: research
MALAT1 regulates hypertrophy of cardiomyocytes by modulating the miR-181a/HMGB2 pathway
Noncoding RNAs are important for regulation of cardiac hypertrophy. The function of MALAT1 (a long noncoding mRNA), miR-181a, and HMGB2; their contribution to cardiac hypertrophy; and the regulatory relationship between them during this process remain unknown. In the present study, we treated primary cardiomyocytes with angiotensin II (Ang II) to mimic cardiac hypertrophy. MALAT1 expression was significantly downregulated in Ang II-treated cardiomyocytes compared with control cardiomyocytes. Ang II-induced cardiac hypertrophy was suppressed by overexpression of MALAT1 and promoted by genetic knockdown of MALAT1. A dual-luc...
Source: European Journal of Histochemistry - June 21, 2022 Category: Biomedical Science Authors: Feng Chen, Wenfeng Li, Dandan Zhang, Youlin Fu, Wenjin Yuan, Gang Luo, Fuwei Liu, Jun Luo Source Type: research
Endoplasmic reticulum stress contributed to inflammatory bowel disease by activating p38 MAPK pathway
In conclusion, ER str ess promotes inflammatory response in IBD, and the effects may be mediated by the activation of p38 MAPK signaling pathway. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - May 23, 2022 Category: Biomedical Science Authors: Yan Long, Yan Zhao, Xiaoqing Ma, Ya Zeng, Tian Hu, Weijie Wu, Chongtian Deng, Jinyue Hu, Yueming Shen Source Type: research
Seasonal changes in the expression of PACAP, VPAC1, VPAC2, PAC1 and testicular activity in the testis of the muskrat ( < em > Ondatra zibethicus < /em > )
In this study, we investigated the seasonal expressions of PACAP, PAC1, VPAC1, VPAC2, luteinizing hormone receptor (LHR), follicle stimulating hormone receptor (FSHR), steroidogenic acute regulatory protein (StAR), 3 β-hydroxysteroid dehydrogenase (3β-HSD), and CYP17A1 in the testis of the male muskrat during the breeding season and the non-breeding season. Histologically, we found the presence of Leydig cells, Sertoli cells and all kinds of germ cells in the testis during the breeding season but only Leydig c ells, Sertoli cells, spermatogonia and primary spermatocyte during the non-breeding season. The immunohistochemi...
Source: European Journal of Histochemistry - May 2, 2022 Category: Biomedical Science Authors: Zeqi Tang, Xiaojie Yuan, Yuming Bai, Yiming Guo, Haolin Zhang, Yingying Han, Zhengrong Yuan, Qiang Weng Source Type: research
Immunohistochemical expression and clinicopathological assessment of PD-1, PD-L1, NY-ESO-1, and MAGE-A4 expression in highly aggressive soft tissue sarcomas
Immunotherapy has altered the treatment paradigm for soft tissue sarcomas (STSs). Considering the limited information regarding the clinical significance of immunohistochemical markers in STS, the purpose of this study was to determine the clinical significance of programmed cell death-1 (PD-1), PD ligand-1 (PD-L1), New York esophageal squamous cell carcinoma-1 (NY-ESO-1), and melanoma-associated antigen-A4 (MAGE-A4) expression in STSs. Twenty-two patients (median age, 72.5 years) with STSs treated at our hospital were included in this study. The specimens obtained at the time of biopsy were used to perform immunostaining ...
Source: European Journal of Histochemistry - April 22, 2022 Category: Biomedical Science Authors: Kazuhiko Hashimoto, Shunji Nishimura, Tomohiko Ito, Ryosuke Kakinoki, Masao Akagi Source Type: research
Histochemistry for nucleic acid research: 60 years in the European Journal of Histochemistry
Since the discovery of DNA structure in 1953, the deoxyribonucleic acid has always been playing a central role in biological research. As physical and ordered nucleotides sequence, it stands at the base of genes existence. Furthermore, beside this 2-dimensional sequence, DNA is characterized by a 3D structural and functional organization, which is of interest for the scientific community due to multiple levels of expression regulation, of interaction with other biomolecules, and much more. Analogously, the nucleic acid counterpart of DNA, RNA, represents a central issue in research, because of its fundamental role in gene ...
Source: European Journal of Histochemistry - April 20, 2022 Category: Biomedical Science Authors: Claudio Casali, Stella Siciliani, Lorena Zannino, Marco Biggiogera Source Type: research
Mammaglobin, GATA-binding protein 3 (GATA3), and epithelial growth factor receptor (EGFR) expression in different breast cancer subtypes and their clinical significance
This study aimed to evaluate mammaglobin, GATA3, and EGFR expression in different breast cancer subtypes, as well as their clinical significance. Subjects of the study included 228 patients with breast cancer at The First Affiliated Hospital of University of Science and Technology of China. They were divided into triple negative (TN), Luminal A, Luminal B, and HER-2 positive (HER-2.P) breast cancer groups based on molecular classification. Immunohistochemical methods were used to detect mammaglobin, GATA3, and EGFR expression in cases of different molecular subtypes before determining the correlation between protein expres...
Source: European Journal of Histochemistry - April 7, 2022 Category: Biomedical Science Authors: Xin Kong, Qi Wang, Jie Li, Ming Li, Fusheng Deng, Chuanying Li Source Type: research
Genome size evaluations in cockroaches: new entries
In this paper, we report genome size (GS) values for nine cockroaches (order Blattodea, families Blattidae, Blaberidae and Ectobiidae, ex Blattelidae,), three of which are original additions to the ten already present in the GS database: the death ’s head roach (Blaberus craniifer), the Surinam cockroach (Pycnoscelus surinamensis) and the Madeira cockroach (Leucophaea maderae). Regarding the American cockroach (Periplaneta americana), the GS database contains two contrasting values (2.72 vs 3.41 pg); likely, the 2.72 pg value is the correct one as it is strikingly similar to our sperm DNA content evaluation (2.80 ± 0.11...
Source: European Journal of Histochemistry - March 24, 2022 Category: Biomedical Science Authors: Manuela Monti, CarloAlberto Redi, Ernesto Capanna Source Type: research
Colorimetric and fluorescent TRAP assays for visualising and quantifying fish osteoclast activity
Histochemical detection of tartrate-resistant acid phosphatase (TRAP) activity is a fundamental technique for visualizing osteoclastic bone resorption and assessing osteoclast activity status in tissues. This approach has mostly employed colorimetric detection, which has limited quantification of activityin situ and co-labelling with other skeletal markers. Here we report simple colorimetric and fluorescent TRAP assays in zebrafish and medaka, two important model organisms for investigating the pathogenesis of bone disorders. We show fluorescent TRAP staining, utilising the ELF97 substrate, is a rapid, robust and stable sy...
Source: European Journal of Histochemistry - March 24, 2022 Category: Biomedical Science Authors: Lalith Prabha Ethiraj, En Lei Samuel Fong, Ranran Liu, Madelynn Chan, Christoph Winkler, Tom James Carney Source Type: research
MiR-130a-5p contributed to the progression of endothelial cell injury by regulating FAS
In conclusion, we detected that miR-130a-5p contributed to the progression of endothelial cell injury by regulating of FAS, which may provide a new and promising therapeutic target for AS. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - March 24, 2022 Category: Biomedical Science Authors: Wei Wang, Wenbo Tang, Erbo Shan, Lei Zhang, Shiyuan Chen, Chaowen Yu, Yong Gao Source Type: research
microRNA-16-5p suppresses cell proliferation and angiogenesis in colorectal cancer by negatively regulating forkhead box K1 to block the PI3K/Akt/mTOR pathway
In conclusion, miR-16-5p hampers cell angiogenesis and proliferation during CRC by targeting FOXK1 to block the PI3K/Akt/mTOR pathway. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - March 24, 2022 Category: Biomedical Science Authors: Xin Huang, Xuan Xu, Huajing Ke, Xiaolin Pan, Jiaoyu Ai, Ruyi Xie, Guilian Lan, Yang Hu, Yao Wu Source Type: research
Secreted key regulators (Fgf1, Bmp4, Gdf3) are expressed by PAC1-immunopositive retinal ganglion cells in the postnatal rat retina
Identified as a member of the secretin/glucagon/VIP superfamily, pituitary adenylate cyclase-activating polypeptide (PACAP1-38) has been recognized as a hormone, neurohormone, transmitter, trophic factor, and known to be involved in diverse and multiple developmental processes. PACAP1-38 was reported to regulate the production of important morphogens (Fgf1, Bmp4, Gdf3) through PAC1-receptor in the newborn rat retina. To follow up, we aimed to reveal the identity of retinal cells responsible for the production and secretion of Fgf1, Bmp4, and Gdf3 in response to PACAP1-38 treatment. Newborn (P1) rats were treated with 100 p...
Source: European Journal of Histochemistry - March 24, 2022 Category: Biomedical Science Authors: Vikt ória Dénes, Kármen Kovacs, Ákos Lukáts, Adrienn Mester, Gergely Berta, Arnold Szabó, Robert Gabriel Source Type: research
Postnatal development of thalamic reticular nucleus projections to the anterior thalamic nuclei in rats
The thalamic reticular nucleus (TRN) projects inhibitory signals to the thalamus, thereby controlling thalamocortical connections. Few studies have examined the development of TRN projections to the anterior thalamic nuclei with regard to axon course and the axon terminal distributions. In the present study, we used parvalbumin (PV) immunostaining to investigate inhibitory projections from the TRN to the thalamus in postnatal (P) 2- to 5-week-old rats (P14 –35). The distribution of PV-positive (+) nerve fibers and nerve terminals markedly differed among the anterior thalamic nuclei at P14. Small, beaded nerve terminals w...
Source: European Journal of Histochemistry - March 24, 2022 Category: Biomedical Science Authors: Hitoshi Fujita, Kosuke Imura, Masahito Takiguchi, Kengo Funakoshi Source Type: research
Stretching prior to resistance training promotes adaptations on the postsynaptic region in different myofiber types
In conclusion, the neuromuscular system ’s components responded according to the myofiber type even though it is the same physical exercise. Besides, static stretching (isolated or combined) plays a crucial role in neuromuscular adaptations. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - February 15, 2022 Category: Biomedical Science Authors: Carolina dos Santos Jacob, Gabriela Klein Barbosa, Mariana Pasquini Rodrigues, Jurandyr Pimentel Neto, Lara Caetano Rocha, Adriano Polican Ciena Source Type: research
Seasonal expressions of GPR41 and GPR43 in the colon of the wild ground squirrels ( < em > Spermophilus dauricus < /em > )
G-protein-coupled receptor 41 (GPR41) and G-protein-coupled receptor 43 (GPR43) are important short-chain fatty acids (SCFAs) receptors. Previous studies indicated that GPR41 and GPR43 are involved in the secretion of gastrointestinal peptides, and glucose and lipid metabolism, and are closely related to obesity and type II diabetes, and other diseases. The purpose of the study was to explore the relationship between the GPR41 and GPR43 and seasonal breeding, and provide new prospects for further exploring the nutritional needs of breeding. We identified the localization and expression levels of GPR41 and GPR43 in the colo...
Source: European Journal of Histochemistry - January 21, 2022 Category: Biomedical Science Authors: Xiaoying Yang, Xuhao Liu, Fengcheng Song, Hao Wei, Fuli Gao, Haolin Zhang, Yingying Han, Qiang Weng, Zhengrong Yuan Source Type: research
