Expression and clinical significance of HER2/neu, aromatase P450 and adhesion molecule CD24 in endometrial cancer
In conclusion, aromatase P450 may be one factor associated with endometrial cancer cell proliferation, while CD24 and HER2/neu may be important factors associated with the invasion and metastasis of endometrial cancer. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - July 26, 2023 Category: Biomedical Science Authors: Liyun Guan, Ying Wang, Jianxin Cheng, Jun Zhang, Shan Kang Source Type: research
Quercetin reverses 5-fluorouracil resistance in colon cancer cells by modulating the NRF2/HO-1 pathway
Quercetin (Que) has been proven to enhance the chemosensitivity of multiple cancers, including colon cancer (CC). However, whether the combination of Que and 5-fluorouracil (5-FU) has a synergistic effect on drug-resistant CC cells has not previously been reported. The effect of Que (5 and 10 μg/mL) on cell vitality and apoptosis of CC and CC drug-resistant cells was examined using a cell counting kit-8 (CCK-8) and flow cytometry. After cells were treated with 5-FU (10, 40 μg/mL), Que (10 μM, 40 μM), or 5-FU in combination with Que, cell proliferation, apoptosis, oxidative stress-rel ated factors, reactive oxygen speci...
Source: European Journal of Histochemistry - July 26, 2023 Category: Biomedical Science Authors: Zhongzhu Tang, Lei Wang, Yunwang Chen, Xiaomin Zheng, Runyu Wang, Bingxue Liu, Shiqi Zhang, Huimin Wang Source Type: research
Tumor cells-derived exosomal PD-L1 promotes the growth and invasion of lung cancer cells < em > in vitro via < /em > mediating macrophages M2 polarization
Lung cancer originating from the bronchial epithelium is the most common lung malignancy. It has been reported that programmed cell death 1 ligand 1 (PD-L1) and tumor-associated macrophages are closely related to the development of lung cancer. However, whether tumor-derived exosomal PD-L1 could mediate the regulation of macrophage polarization in lung cancer remains unclear. For this research, the level of PD-L1 in normal tissues and lung cancer tissues was evaluated using RT-qPCR. Next, the apoptosis of lung cancer cells was evaluated using flow cytometry assay. Then, the structure and morphology of vesicles were observe...
Source: European Journal of Histochemistry - July 26, 2023 Category: Biomedical Science Authors: Xiangjun Lu, Jian Shen, Siyuan Huang, Dongdong Liu, Haitao Wang Source Type: research
Substance P is overexpressed in cervical squamous cell carcinoma and promoted proliferation and invasion of cervical cancer cells < em > in vitro < /em >
In conclusion, substance P is highly expressed in cervical squamous cell carcinoma and can promote cervical cancer cell proliferation and invasion. The mechanism is related to the activation of ERK1/2 pathway to upregulate MMP9. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - July 26, 2023 Category: Biomedical Science Authors: Ying Wang, Shifa Yuan, Jing Ma, Hong Liu, Lizhen Huang, Fengzhen Zhang Source Type: research
Therapeutic effects of human umbilical cord mesenchymal stem cell-derived extracellular vesicles on ovarian functions through the PI3K/Akt cascade in mice with premature ovarian failure
This study intended to uncover the therapeutic effects of human umbilical cord MSC-derived extracellular vesicles (hucMSCEVs) on POF. hucMSCs were identified by observing morphology and examining differentiation capabilities. EVs were extracted from hucMSCs and later identified utilizing nanoparticle tracking analysis, transmission electron microscopy, and Western blotting. POF mouse models were established by injecting D-galactose (Dgal). The estrous cycles were assessed through vaginal cytology, and serum levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), anti-mullerian hormone (AMH), estradiol (E2),...
Source: European Journal of Histochemistry - July 26, 2023 Category: Biomedical Science Authors: Nan Li, Xue Fan, Lihong Liu, Yanbing Liu Source Type: research
Dexmedetomidine attenuates neuroinflammation and microglia activation in LPS-stimulated BV2 microglia cells through targeting circ-Shank3/miR-140-3p/TLR4 axis
In this study, we found that Dex notably decreased circ-Shank3 and TLR4 levels and elevated miR-140-3p level in LPS-treated BV2 cells. Mechanistically, circ-Shank3 harbor miR-140-3p, functioning as a miRNA sponge, and then miR-140-3p targeted the 3 ’-UTR of TLR4. Additionally, Dex treatment significantly reduced TLR4 level and phosphorylation of p65, and decreased the expressions of microglia markers Iba-1 and CD11b in LPS-treated BV2 cells. As expected, silenced circ-Shank3 further reduced TLR4, p65 and Iba-1 and CD11b levels in LPS-treated BV2 cells in the presence of Dex, whereas these phenomena were reversed by miR-1...
Source: European Journal of Histochemistry - July 26, 2023 Category: Biomedical Science Authors: Guangbao He, Yibo He, Hongwei Ni, Kai Wang, Yijun Zhu, Yang Bao Source Type: research
Deletion of osteopontin in non-small cell lung cancer cells affects bone metabolism by regulating miR-34c/Notch1 axis: a clue to bone metastasis
Lung cancer is prone to bone metastasis, and osteopontin (OPN) has an important significance in maintaining bone homeostasis. The goal of this study was to explore the impact of OPN level on bone metabolism and the molecular mechanism of inhibiting bone metastasis in non-small cell lung cancer (NSCLC). The expression of OPN in NSCLC was ascertained by Western blot and immunohistochemistry, and the correlation between the expression level of OPN and survival of patients was analyzed. Then the shRNA technology was applied to reduce the expression of OPN in NSCLC cells, and CCK-8 assay was carried out to investigate the effec...
Source: European Journal of Histochemistry - July 26, 2023 Category: Biomedical Science Authors: Jing Guo, Chang-Yong Tong, Jian-Guang Shi, Xin-Jian Li, Xue-Qin Chen Source Type: research
Erratum - Transcription factor nuclear factor erythroid 2 p45-related factor 2 (NRF2) ameliorates sepsis-associated acute kidney injury by maintaining mitochondrial homeostasis and improving the mitochondrial function
This corrects the article published inEuropean Journal of Histochemistry 2022;66:3412. doi: 10.4081/ejh.2022.3412. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - June 20, 2023 Category: Biomedical Science Authors: Zhijiang Chen, Huili Wang, Bin Hu, Xinxin Chen, Meiyu Zheng, Lili Liang, Juanjuan Lyu, Qiyi Zeng Source Type: research
Proceedings of the workshop NANO23@uniVR - 8-9 June 2023, University of Verona, Italy
The workshop, organized by thePhD Course in Nanosciences andAdvanced Technologies, aims to create a forum on hot topics of current interest in Nanoscience and Nanotechnology such as Nanomedicine, Biotechnology, Energy-nanotech, Environmental nanoscience, Green nanotechnology, Nanoengineering. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - May 31, 2023 Category: Biomedical Science Authors: The Scientific Committee Source Type: research
LncRNA gadd7 promotes mitochondrial membrane potential decrease and apoptosis of alveolar type II epithelial cells by positively regulating MFN1 in an < em > in vitro < /em > model of hyperoxia-induced acute lung injury
Hyperoxia-induced acute lung injury (HALI) is a complication of oxygen therapy. LncRNA gadd7 shows associations with HALI. We explored the effects of gadd7 on mitochondrial membrane potential (MMP) and apoptosis of alveolar type II epithelial cells (AEC II) in HALI. RLE-6TN cells were transfected with 50 nM silenced and overexpressed gadd7 and MFN1 plasmids (sh-gadd7, oe-gadd7 and/or oe-MFN1), respectively for 48 h, followed by hyperoxic culture for 24 h. gadd7 and MFN1 levels were assessed by RT-qPCR and Western blot. RLE-6TN cells were stained by JC-1 and MMP changes were observed using a fluorescence microscope. The ave...
Source: European Journal of Histochemistry - May 31, 2023 Category: Biomedical Science Authors: Guoyue Liu, Cunzhi Yin, Mingjiang Qian, Xuan Xiao, Hang Wu, Fujian Fu Source Type: research
High glucose inhibits neural differentiation by excessive autophagy < em > via < /em > peroxisome proliferator-activated receptor gamma
The high prevalence of prediabetes and diabetes globally has led to the widespread occurrence of severe complications, such as diabetic neuropathy, which is a result of chronic hyperglycemia. Studies have demonstrated that maternal diabetes can lead to neural tube defects by suppressing neurogenesis during neuroepithelium development. While aberrant autophagy has been associated with abnormal neuronal differentiation, the mechanism by which high glucose suppresses neural differentiation in stem cells remains unclear. Therefore, we developed a neuronal cell differentiation model of retinoic acid induced P19 cells to investi...
Source: European Journal of Histochemistry - May 11, 2023 Category: Biomedical Science Authors: Yin Pan, Di Qiu, Shu Chen, Xiaoxue Han, Ruiman Li Source Type: research
LncRNA FBXO18-AS promotes gastric cancer progression by TGF- β1/Smad signaling
For the digestive system, there exists one common malignant tumor, known as gastric cancer. It is the third most prevalent type of tumor among different tumors worldwide. It has been reported that long noncoding RNAs (lncRNAs), participate in various biological processes of gastric cancer. However, there are still many lncRNAs with unknown functions, and we discovered a novel lncRNA designated as FBXO18-AS. Whether lncRNAFBXO18-AS participates in gastric cancer progression is still unknown. Bioinformatic analysis, immunohistochemistry, Western blotting, and qPCR were carried out to explore FBXO18-AS and TGF- β1 expression...
Source: European Journal of Histochemistry - March 20, 2023 Category: Biomedical Science Authors: Yiming Zhang, Wanqiong Zheng, Liang Zhang, Yechun Gu, Lihe Zhu, Yingpeng Huang Source Type: research
Sodium hyaluronate promotes proliferation, autophagy, and migration of corneal epithelial cells by downregulating miR-18a in the course of corneal epithelial injury
Corneal epithelium can resist the invasion of external pathogenic factors to protect the eye from external pathogens. Sodium hyaluronate (SH) has been confirmed to promote corneal epithelial wound healing. However, the mechanism by which SH protects against corneal epithelial injury (CEI) is not fully understood. CEI model mice were made by scratching the mouse corneal epithelium, andin vitro model of CEI were constructed via curettage of corneal epithelium or ultraviolet radiation. The pathologic structure and level of connective tissue growth factor (CTGF) expression were confirmed by Hematoxylin and Eosin staining and i...
Source: European Journal of Histochemistry - March 20, 2023 Category: Biomedical Science Authors: Yingzhuo Guo, Hua Wang Source Type: research
Metformin sensitises osteosarcoma to chemotherapy < em > via < /em > the IGF-1R/miR-610/FEN1 pathway
Metformin can enhance cancer cell chemosensitivity to anticancer drugs. IGF-1R is involved in cancer chemoresistance. The current study aimed to elucidate the role of metformin in osteosarcoma (OS) cell chemosensitivity modulation and identify its underlying mechanism in IGF-1R/miR-610/FEN1 signalling. IGF-1R, miR-610, and FEN1 were aberrantly expressed in OS and participated in apoptosis modulation; this effect was abated by metformin treatment. Luciferase reporter assays confirmed that FEN1 is a direct target of miR-610. Moreover, metformin treatment decreased IGF-1R and FEN1 but elevated miR-610 expression. Metformin se...
Source: European Journal of Histochemistry - March 20, 2023 Category: Biomedical Science Authors: Suwei Dong, Yanbin Xiao, Ziqiang Zhu, Xiang Ma, Zhuohui Peng, Jianping Kang, Jianqiang Wang, Yunqing Wang, Zhen Li Source Type: research
Knockdown of ARHGAP30 inhibits ovarian cancer cell proliferation, migration, and invasiveness by suppressing the PI3K/AKT/mTOR signaling pathway
In conclusion, ARHGAP30 regulated the PI3K/AKT/mTOR pathway to promote progression of OC. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - March 20, 2023 Category: Biomedical Science Authors: Xiaoyan Chu, Jun Lou, Yun Yi, Linlin Zhong, Ouping Huang Source Type: research
