Autofluorescence in freshly isolated adult human liver sinusoidal cells
Autofluorescent granules of various sizes were observed in primary human liver endothelial cells (LSECs) upon laser irradiation using a wide range of wavelengths. Autofluorescence was detected in LAMP-1 positive vesicles, suggesting lysosomal location. Confocal imaging of freshly prepared cultures and imaging flow cytometry of non-cultured cells revealed fluorescence in all channels used. Treatment with a lipofuscin autofluorescence quencher reduced autofluorescence, most efficiently in the near UV-area. These results, combined with the knowledge of the very active blood clearance function of LSECs support the notion that ...
Source: European Journal of Histochemistry - November 2, 2021 Category: Biomedical Science Authors: Anett Kristin Larsen, Jaione Sim ón-Santamaría, Kjetil Elvevold, Bo Göran Ericzon, Kim Erlend Mortensen, Peter McCourt, Bård Smedsrød, Karen Kristine Sørensen Source Type: research
Different fusion tags affect the activity of ubiquitin overexpression on spastin protein stability
Spastin is one of the proteins which lead to hereditary spastic paraplegia (HSP), whose dysfunction towards microtubule severing and membrane transporting is critically important. The present study is to elucidate the mechanisms of the protein stability regulation of spastin. The ubiquitin encoding plasmids are transfected into COS-7 cells with different fusion tags including Green Fluorescent Protein (GFP), mCherry and Flag. The expression level of spastin was detected, microtubule severing activity and neurite outgrowth were quantified. The data showed that ubiquitin overexpression significantly induced the decreased exp...
Source: European Journal of Histochemistry - November 2, 2021 Category: Biomedical Science Authors: Jianyu Zou, Zhenbin Cai, Zhi Liang , Yaozhong Liang, Guowei Zhang, Jie Yang, Yunlong Zhang, Hongsheng Lin, Minghui Tan Source Type: research
Mucin1 relieves acute lung injury by inhibiting inflammation and oxidative stress
We examined the effect of MUC1 on ALI/ARDS in mice by detecting the expression of inflammatory factors and oxidative stress molecules in mouse lung tissue, bronchoalveolar lavage fluid (BALF) and serum. Overexpression of MUC1 effectively ameliorated LPS-induced damage to BEAS-2B cells. Results of H&E staining indicate that LPS successfully induced ALI/ARDS in mice and MUC1 attenuated lung injury. MUC1 also reduced the expression of inflammatory factors (IL-1 β, TNF-α, IL-6 and IL-8) and oxidative stress levels in mice. In addition, LPS results in an increase in the activity of the TLR4/NF-κB signaling pathway in mic...
Source: European Journal of Histochemistry - November 2, 2021 Category: Biomedical Science Authors: Chunlin Ye, Bin Xu, Jie Yang, Yunkun Liu, Zhikai Zeng, Lingchun Xia, Quanjin Li, Guowen Zou Source Type: research
The MEG3 lncRNA promotes trophoblastic cell growth and invasiveness in preeclampsia by acting as a sponge for miR-21, which regulates BMPR2 levels
This study aimed to investigate the potential impact and regulatory mechanisms of bone morphogenetic protein receptor 2 (BMPR2) on the progression of PE. We obtained placental tissues from pregnant women with PE and normal pregnant women, and the results showed that BMPR2 was expressed at low levels in the tissue from PE women. Genetic knockdown of BMPR2 increased the proliferation and invasion of cultured trophoblast cells, whereas its overexpression reduced these characteristics. Bioinformatics analysis and luciferase reporter gene assays confirmed thatBMPR2 is a direct target of miR-21. Overexpression of a miR-21 inhibi...
Source: European Journal of Histochemistry - November 2, 2021 Category: Biomedical Science Authors: Huyi Liu, Xiangdao Cai , Jia Liu, Fengxiang Zhang , Andong He, Ruiman Li Source Type: research
Downregulation of Williams syndrome transcription factor (WSTF) suppresses glioblastoma cell growth and invasion by inhibiting PI3K/AKT signal pathway
In conclusion, WSTF contributed to GBM cell growth and invasion through activation of PI3K/AKT pathway. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - November 2, 2021 Category: Biomedical Science Authors: Liyuan Yang, Chunfu Du, Hui Chen, Zhengwen Diao Source Type: research
Correlation of the AKT/mTOR signaling pathway with the clinicopathological features and prognosis of nasopharyngeal carcinoma
The primary aim of this study was to examine the correlation of the AKT/mTOR signaling pathway with the clinicopathological features and prognostic significance in nasopharyngeal carcinoma (NPC). The study tissues were collected from 285 patients with NPC and normal mucosal tissues were obtained from 289 individuals with normal nasopharynxes. Immunohistochemical staining was used to detected the expression of the AKT, mTOR, andp70 ribosomal S6 kinase(P70S6K) proteins. Follow-up was performed for between 8 and 60 months. Spearman ’s rank correlation analysis was performed to evaluate the correlation of the expression of t...
Source: European Journal of Histochemistry - November 2, 2021 Category: Biomedical Science Authors: Yan Wang, Jie Sun, Ninghua Yao Source Type: research
Distribution of α-synuclein in normal human jejunum and its relations with the chemosensory and neuroendocrine system
Alpha-synuclein ( α-syn) is a presynaptic neuronal protein and its structural alterations play an important role in the pathogenesis of neurodegenerative diseases, such as Parkinson’s disease (PD). It has been originally described in the brain and aggregated α-syn has also been found in the peripheral nerves incl uding the enteric nervous system (ENS) of PD patients. ENS is a network of neurons and glia found in the gut wall which controls gastrointestinal function independently from the central nervous system. Moreover, two types of epithelial cells are crucial in the creation of an interface between the lu men and th...
Source: European Journal of Histochemistry - November 2, 2021 Category: Biomedical Science Authors: Arianna Casini, Romina Mancinelli, Caterina Loredana Mammola, Luigi Pannarale, Piero Chirletti, Paolo Onori, Rosa Vaccaro Source Type: research
Distribution of alpha-synuclein in normal human jejunum and its relations with the chemosensory and neuroendocrine system
Alpha-synuclein ( α-syn) is a presynaptic neuronal protein and its structural alterations play an important role in the pathogenesis of neurodegenerative diseases, such as Parkinson’s disease (PD). It has been originally described in the brain and aggregated α-syn has also been found in the peripheral nerves incl uding the enteric nervous system (ENS) of PD patients. ENS is a network of neurons and glia found in the gut wall which controls gastrointestinal function independently from the central nervous system. Moreover, two types of epithelial cells are crucial in the creation of an interface between the lu men and th...
Source: European Journal of Histochemistry - November 2, 2021 Category: Biomedical Science Authors: Arianna Casini, Romina Mancinelli, Caterina Loredana Mammola, Luigi Pannarale, Piero Chirletti, Paolo Onori, Rosa Vaccaro Source Type: research
Morphological and histochemical characterization of the secretory epithelium in the canine lacrimal gland
In the present study, the expression of secretory components and vesicular transport proteins in the canine lacrimal gland was examined and morphometric analysis was performed. The secretory epithelium consists of two types of secretory cells with different morphological features. The secretory cells constituting acinar units (type A cells) exhibited higher levels of glycoconjugates, including β-GlcNAc, than the other cell type constituting tubular units (type T cells). Immunoblot analysis revealed that antimicrobial proteins, such as lysozyme, lactoferrin and lactoperoxidase, Rab proteins (Rab3d, Rab27a and Rab27b) and s...
Source: European Journal of Histochemistry - November 2, 2021 Category: Biomedical Science Authors: Tadashi Yasui, Kenya Miyata, Chie Nakatsuka, Azuma Tsukise, Hiroshi Gomi Source Type: research
Identification of abnormally high expression of POGZ as a new biomarker associated with a poor prognosis in osteosarcoma
Osteosarcoma (OS) is the most prevalent malignant bone tumor in children and young adults. There is an urgent need for a novel biomarker related to the prognosis of OS. We performed a meta-analysis incorporating six independent datasets and performed a survival analysis with one independent dataset GSE21257 in the GEO database for gene screening. The results revealed that one potential biomarker related to OS survival,POGZ was the most significantly upregulated gene. We also verified that thePOGZ was overexpressed in clinical samples. The survival analysis revealed thatPOGZ is associated with a poor prognosis in OS. Moreov...
Source: European Journal of Histochemistry - September 3, 2021 Category: Biomedical Science Authors: Sikuan Zheng, Yue Liu, Haohe Sun, Jingyu Jia, Tianlong Wu, Rui Ding, Xigao Cheng Source Type: research
Long noncoding RNA Meg3 mediates ferroptosis induced by oxygen and glucose deprivation combined with hyperglycemia in rat brain microvascular endothelial cells, through modulating the p53/GPX4 axis
In this study, rat brain microvascular endothelial cells (RBMVECs) were exposed to 6 h of oxygen and glucose deprivation (OGD), and subsequent reperfusionvia incubating cells with glucose of various high concentrations for 24 h to imitateinvitro diabetic brain ischemic injury. It was shown that the marker events of ferroptosis and increasedMeg3 expression occurred after the injury induced by OGD combined with hyperglycemia. However, all ferroptotic events were reversed with the treatment ofMeg3-siRNA. Moreover, in this in vitro model, p53 was also characterized as a downstream target ofMeg3. Furthermore, p53 knockdown prot...
Source: European Journal of Histochemistry - July 2, 2021 Category: Biomedical Science Authors: Cheng Chen, Yan Huang, Pingping Xia, Fan Zhang, Longyan Li, E Wang, Qulian Guo, Zhi Ye Source Type: research
miR-29a-3p enhances the radiosensitivity of oral squamous cell carcinoma cells by inhibiting ADAM12
This study aims to determine the role and function of miR-29a-3p and ADAM12 in the radiosensitivity of OSCC cells. The expression pattern of ADAM12 in OSCC cells was searched in TCGA database. The binding of miR-29a-3p and ADAM12 was predicted by Starbase and verified using dual luciferase reporter gene assay. The RNA or protein expressions of miR-29a-3p and ADAM12 were measured by RT-qPCR or western blot. OSCC cell lines were treated by various γ-ray irradiation dosages before the alteration on miR-29a-3p expression and on the cell viability, proliferation, migration and cell apoptosis was detected. ADAM12 was highly exp...
Source: European Journal of Histochemistry - July 2, 2021 Category: Biomedical Science Authors: Cuihong Jiang, Feng Liu , Shuai Xiao, Lili He, Wenqiong Wu, Qi Zhao Source Type: research
miRNA-877-5p inhibits malignant progression of prostate cancer by directly targeting SSFA2
In this study, we aimed to investigate the role of miR-877-5p in the malignant phenotypes of prostate cancer (PCa) cells and its underlying mechanism. RT-qPCR analysis was performed to examine the expression of miR-877-5p and sperm-specific antigen 2 (SSFA2) in PCa tissues and cells. Cell counting kit-8 (CCK-8) assay, 5-ethynyl-20-deoxyuridine (EdU) assay, flow cytometry, wound-healing assay, and Transwell invasion assay were performed to determine the functional roles of miR-877-5p in PCa cells. The association of miR-877-5p with SSFA2 was determined by luciferase reporter and RNA pull-down assays. In this study, we found...
Source: European Journal of Histochemistry - July 2, 2021 Category: Biomedical Science Authors: Wanchun Wang, Jun Yi, Degang Dong, Wenli Mao, Xuanyu Wang, Zhangren Yan Source Type: research
Long noncoding RNA H19 accelerates tenogenic differentiation by modulating miR-140-5p/VEGFA signaling
Rotator cuff tear (RCT) is a common tendon injury, but the mechanisms of tendon healing remain incompletely understood. Elucidating the molecular mechanisms of tenogenic differentiation is essential to develop novel therapeutic strategies in clinical treatment of RCT. The long noncoding RNA H19 plays a regulatory role in tenogenic differentiation and tendon healing, but its detailed mechanism of action remains unknown. To elucidate the role of H19 in tenogenic differentiation and tendon healing, tendon-derived stem cells were harvested from the Achilles tendons of Sprague Dawley rats and a rat model of cuff tear was e...
Source: European Journal of Histochemistry - July 2, 2021 Category: Biomedical Science Authors: You-Jie Liu, Hua-Jun Wang, Zhao-Wen Xue, Lek-Hang Cheang, Man-Seng Tam, Ri-Wang Li, Jie-Ruo Li, Hui-Ge Hou, Xiao-Fei Zheng Source Type: research
Morphological and morphometric changes and epithelial apoptosis are induced in the rat epididymis by long-term letrozole treatment
The epididymis is an organ that plays a key role in sperm maturation. The aim of this study was to examine the association between the chronic treatment of mature male rats with letrozole and morphological evaluation and morphometric values of epididymis as well as changes in the number of apoptotic cells in epididymal epithelium. Adult rats were treated with letrozole for 6 months and the epididymis weight, morphology, morphometric values and the number of apoptotic cells in the epithelium were examined. Long-term aromatase inhibition resulted in presence of intraepithelial clear vacuoles, hyperplasia of clear cells ...
Source: European Journal of Histochemistry - July 2, 2021 Category: Biomedical Science Authors: Anna Pilutin, Kamila Misiakiewicz-Has, Sylwia Rzeszotek, Barbara Wiszniewska Source Type: research
