Phosphorylation mutation impairs the promoting effect of spastin on neurite outgrowth without affecting its microtubule severing ability
In conclusion, the data suggest that mutations at multiple phosphorylation sites of spastin do not impair its microtubule cleavage ability in COS7 cells, but reduce its ability to promote neurite outgrowth in rat hippocampal neurons. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - December 22, 2022 Category: Biomedical Science Authors: Yunlong Zhang, Xin He, Jianyu Zou, Jie Yang, Ao Ma, Minghui Tan Source Type: research
An < em > ex vivo < /em > experimental system to track fluorescent nanoparticles inside skeletal muscle
The development of novel nanoconstructs for biomedical applications requires the assessment of their biodistribution, metabolism and clearance in single cells, organs and entire organisms in a living environment. To reduce the number ofin vivo experiments performed and to refine the methods used, in accordance with the 3Rs principle, this work proposes anex vivo experimental system to monitor, using fluorescence microscopy, the distribution of nanoparticles in explanted murine skeletal muscle maintained in a bioreactor that can preserve the structural and functional features of the organ for long periods of time. Fluoresce...
Source: European Journal of Histochemistry - December 22, 2022 Category: Biomedical Science Authors: Laura Calderan, Flavia Carton, Ilaria Andreana, Valeria Bincoletto, Silvia Arpicco, Barbara Stella, Manuela Malatesta Source Type: research
DPY30 promotes the growth and survival of osteosarcoma cell by regulating the PI3K/AKT signal pathway
We reported an upregulation of DPY30 in OS tumor tissues in both published dataset and clinical samples. A high level of DPY30 expression was associated with larger tumor size and more metastasis in OS patients, as well as poor overall survival. DPY30 knockdown in OS cells significantly impairs proliferation, migration and invasion, but induced cellular apoptosis. We further demonstrated that the agonist of PI3K/AKT pathway can rescue the inhibitory effects of DPY30 knockdown in OS cells. Together, our data indicate that DPY30 functions as an oncogene to promote the malignancy of OS cells possibly through PI3K/AKT pathway....
Source: European Journal of Histochemistry - December 22, 2022 Category: Biomedical Science Authors: Gong Cheng, Fengmin An, Zhilin Cao, Mingdi Zheng, Zhongyuan Zhao, Hao Wu Source Type: research
Differential antigen expression between human apocrine sweat glands and eccrine sweat glands
Bromhidrosis has a great negative impact on personal occupation and social psychology. It is not yet clear whether bromhidrosis is caused by apocrine sweat glands or the co-action of apocrine sweat glands and eccrine sweat glands. To distinguish between apocrine sweat glands and eccrine sweat glands, specific antigen markers for apocrine sweat glands and eccrine sweat glands must be found first. In the study, we detected the expression of K7, K18, K19, Na+-K+-2Cl- cotransporter 1 (NKCC1), carbonic anhydrase II (CAII), Forkhead transcription factor a1 (Foxa1), homeobox transcription factor engrailed homeobox1 (En1), gross c...
Source: European Journal of Histochemistry - December 22, 2022 Category: Biomedical Science Authors: Manxiu Cao, Lei Zhang, Jiaqi Chen, Cangyu Wang, Junhong Zhao, Xiang Liu, Yongjing Yan, Yue Tang, Zixiu Chen, Haihong Li Source Type: research
Decreased VEGFA alleviates the symptoms of LPS-induced sepsis in a mouse model by inhibiting glycolysis and thereby regulating the polarization of macrophages
This study demonstrates that decreased VEGFA inhibits glycolysis and thus inhibits LPS-induced M1 polarization of macrophages, ultimately relieving sepsis. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - December 22, 2022 Category: Biomedical Science Authors: Jun Lin, Liping Jiang, Kun Guo, Ning Feng Source Type: research
Expression profile of the zinc transporter ZnT3 in taste cells of rat circumvallate papillae and its role in zinc release, a potential mechanism for taste stimulation
Zinc is an essential trace element, and its deficiency causes taste dysfunction. Zinc accumulates in zinc transporter (ZnT)3-expressing presynaptic vesicles in hippocampal neurons and acts as a neurotransmitter in the central nervous system. However, the distribution of zinc and its role as a signal transmitter in taste buds remain unknown. Therefore, we examined the distribution of zinc and expression profiles of ZnT3 in taste cells and evaluated zinc release from isolated taste cells upon taste stimuli. Taste cells with a spindle or pyriform morphology were revealed by staining with the fluorescent zinc dye ZnAF-2DA and ...
Source: European Journal of Histochemistry - November 10, 2022 Category: Biomedical Science Authors: Kentaro Nishida, Saho Bansho, Akiko Ikukawa, Teruyo Kubota, Akihiro Ohishi, Kazuki Nagasawa Source Type: research
SerpinB3 administration protects liver against ischemia-reperfusion injury
We have investigated the change in SerpinB3 during hepatic ischemia and the potential role of its antiprotease activity in cell protection by the administration of wild-type SerpinB3 (SerpinB3-WT) or active loop-deleted recombinant SerpinB3 protein (SerpinB3-D) in a rat model of ischemia (60 min)/reperfusion (60 min) (I/R). A time-dependent increase of SerpinB3, both at transcription and protein level, was found in ischemic livers after 60, 120 and 180 min. SerpinB3-WT decreased polymorphonuclear cell infiltration and serum enzymes and increased ATP when compared with I/R group. These events were not obtained using SerpinB...
Source: European Journal of Histochemistry - October 28, 2022 Category: Biomedical Science Authors: Cristian Turato, Mariapia Vairetti, Marta Cagna, Alessandra Biasiolo, Andrea Ferrigno, Santina Quarta, Mariagrazia Ruvoletto, Silvia De Siervi, Patrizia Pontisso, Laura Giuseppina Di Pasqua Source Type: research
Chemotherapy-induced nephrotoxicity was improved by crocin in mouse model
In conclusion, crocin protects against CDDP-induced oxidative stress and nephrotoxicity by attenuating the activation of p38 mitogen-activated protein kinase and caspase-3 cleavage. (Source: European Journal of Histochemistry)
Source: European Journal of Histochemistry - October 3, 2022 Category: Biomedical Science Authors: Qichao Yin, Hua Xiong Source Type: research
Assessing the interactions between nanoparticles and biological barriers in vitro: a new challenge for microscopy techniques in nanomedicine
Nanoconstructs intended to be used as biomedical tool must be assessed for their capability to cross biological barriers. However, studyingin vivo the permeability of biological barriers to nanoparticles is quite difficult due to the many structural and functional factors involved. Therefore, thein vitro modeling of biological barriers -2D cell monocultures, 2D/3D cell co-cultures, microfluidic devices- is gaining more and more relevance in nanomedical research. Microscopy techniques play a crucial role in these studies, as they allow both visualizing nanoparticles inside the biological barrier and evaluating their impact ...
Source: European Journal of Histochemistry - September 20, 2022 Category: Biomedical Science Authors: Flavia Carton, Manuela Malatesta Source Type: research
Combination of tolvaptan and valsartan improves cardiac and renal functions in doxorubicin-induced heart failure in mice
In this study, we found that compared to tolvaptan or valsartan alone treatment group, tolvaptan/valsartan combination obviously improved the left ventricular ejection fraction (LVEF) and the left ventricular fractional shortening (LVFS), and reduced serum ALD and Ang II level in Dox-treated mice. Additionally, tolvaptan/valsartan combination significantly prevented the inflammation and fibrosis of heart and kidney tissues in Dox-treated mice. Meanwhile, tolvaptan/valsartan combination notably inhibited the myocardial and renal cell apoptosis in Dox-treated mice via upregulation of Bcl-2 and downregulation of Bax and cleav...
Source: European Journal of Histochemistry - September 20, 2022 Category: Biomedical Science Authors: Fengqin Yan, Hong Zhu, Yingxia He, Qinqin Wu, Xiaoyu Duan Source Type: research
VEGF promotes diabetic retinopathy by upregulating the PKC/ET/NF- κB/ICAM-1 signaling pathway
In this study, we investigated the regulatory role and clinical significance of the vascular endothelial growth factor (VEGF)/protein kinase C (PKC)/endothelin (ET)/nuclear factor- κB (NF-κB)/intercellular adhesion molecule 1 (ICAM-1) signaling pathway in DR using a rat model. Intraperitoneal injections of the VEGF agonist, streptozotocin (STZ) were used to generate the DR model rats. DR rats treated with the VEGF inhibitor (DR+VEGF inhibitor) were used to study the specific effects of VEGF on DR pathology and the underlying mechanisms. DR and DR+VEGF agonist rats were injected with the PKCβ2 inhibitor, GF109203X to det...
Source: European Journal of Histochemistry - September 20, 2022 Category: Biomedical Science Authors: Meiying Zhang, Min Zhou, Xia Cai, Yan Zhou, Xueling Jiang, Yan Luo, Yue Hu, Rong Qiu, Yanrong Wu, Yuejin Zhang, Yan Xiong Source Type: research
Docetaxel-resistant triple-negative breast cancer cell-derived exosomal lncRNA LINC00667 reduces the chemosensitivity of breast cancer cells to docetaxel < em > via < /em > targeting miR-200b-3p/Bcl-2 axis
Development of docetaxel (TXT) resistance is a major obstacle for triple-negative breast cancer (TNBC) treatment. Additionally, chemoresistant cell-derived exosomes were able to change the chemo-response of chemosensitive recipient cellsvia transportation of lncRNAs. It has been shown that lncRNA LINC00667 level was significantly elevated in breast cancer tissues. Therefore, we explored whether LINC00667 level is increased in TXT-resistant TNBC cell-derived exosomes. In addition, whether exosomal LINC00667 derived from TXT-resistant TNBC cell could affect TXT sensitivity in TXT-sensitive TNBC cells was investigated as well...
Source: European Journal of Histochemistry - September 20, 2022 Category: Biomedical Science Authors: Jindong Li, Jie Kang, Weiyan Liu, Jiazhe Liu, Gaofeng Pan, Anwei Mao, Qing Zhang, Jingfeng Lu, Junbin Ding, Hongchang Li Source Type: research
Tumor cell-derived exosomal lncRNA LOC441178 inhibits the tumorigenesis of esophageal carcinoma through suppressing macrophage M2 polarization
Esophageal carcinoma (EC) is a highly malignant type of tumor. In a previous study, the authors found that long non-coding RNA (lncRNA) LOC441178 inhibited the tumorigenesis of EC. Moreover, exosomes derived from tumor cells containing lncRNAs were found to play a key role in the tumor environment; however, whether exosomes can affect the tumor microenvironment by carrying LOC441178 remains unclear. Thus, the present study aimed to clarify this. In order to assess the effects of exosomal LOC441178 in EC, cell invasion and migration were examined using the Transwell assay. Exosomes were identified using transmission electro...
Source: European Journal of Histochemistry - September 20, 2022 Category: Biomedical Science Authors: Jiangmu Chen, Zongchi Chen, Weitao Hu, Daxing Cai Source Type: research
Glyphosate, AMPA and glyphosate-based herbicide exposure leads to GFAP, PCNA and caspase-3 increased immunoreactive area on male offspring rat hypothalamus
Glyphosate, aminomethylphosphonic acid (AMPA), and glyphosate-based herbicides altered the neuroendocrine axis, the content of brain neurotransmitters, and behavior in experimental animal models. Glyphosate alone, AMPA or Roundup® Active were administered to postpartum female rats, from P0 to P10, and their water consumption was measured daily. The immunoreactivity for glial fibrillary acidic protein (GFAP), proliferating cell nuclear antigen (PCNA) and caspase-3 was measured in the anterior, medial preoptic, periventricular, supraoptic and lateroanterior hypothalamic nuclei of P0-P10 male pups after exposure,vialactation...
Source: European Journal of Histochemistry - September 20, 2022 Category: Biomedical Science Authors: Ewing Duque-D íaz, Hernán Hurtado Giraldo, Linda P. Rocha-Muñoz, Rafael Coveñas Source Type: research
Overexpression of hsa_circ_0006470 inhibits the malignant behavior of gastric cancer cells < em > via < /em > regulation of miR-1234/TP53I11 axis
Gastric cancer (GC) is a subtype of a common malignant tumor found in the digestive system. Hsa_circ_0006470 is known to be closely associated with the development of GC. Nevertheless, the mechanism by which hsa_circ_0006470 regulates the tumorigenesis of GC has not been fully elucidated. To investigate the role of hsa_circ_0006470 in GC, its expression levels were assessed in GES-1, AGS, MKN45, and SNU5 cells by reverse transcription-quantitative PCR. Fluorescencein situ hybridization was used to evaluate the localization of hsa_circ_0006470 in AGS and MKN45 cells. In addition, cell counting kit-8 and 5-ethynyl-2 ’-deox...
Source: European Journal of Histochemistry - September 20, 2022 Category: Biomedical Science Authors: Jinbi Xie, Yong Ning, Lihang Zhang, Yuan Lin, Runsheng Guo, Shanjuan Wang Source Type: research
