Identification of abnormally high expression of POGZ as a new biomarker associated with a poor prognosis in osteosarcoma

Osteosarcoma (OS) is the most prevalent malignant bone tumor in children and young adults. There is an urgent need for a novel biomarker related to the prognosis of OS. We performed a meta-analysis incorporating six independent datasets and performed a survival analysis with one independent dataset GSE21257 in the GEO database for gene screening. The results revealed that one potential biomarker related to OS survival,POGZ was the most significantly upregulated gene. We also verified that thePOGZ was overexpressed in clinical samples. The survival analysis revealed thatPOGZ is associated with a poor prognosis in OS. Moreover, flow cytometry analysis of isolated OS cells demonstrated that OS cells were arrested in the G1 phase afterPOGZ knockdown. The RNA-seq results indicated thatPOGZ was co-expressed with CCNE1 and CCNB1. Pathway analysis showed that genes associated with high expression levels ofPOGZ were related to the cell cycle pathway. A cell model was constructed to detect the effects ofPOGZ. AfterPOGZ knockdown, OS cell proliferation, invasion and migration were all decreased. Therefore, POGZ is an important gene for evaluating the prognosis of OS patients and is a potential therapeutic target.
Source: European Journal of Histochemistry - Category: Biomedical Science Authors: Source Type: research