Alcian blue staining to track the intracellular fate of hyaluronic-acid-based nanoparticles at transmission electron microscopy

In this study, the critical-electrolyte-concentration Alcian Blue method set up by Schofieldet al. in 1975 was applied to track hyaluronic-acid-based NPs in muscle cellsin vitro. This long-established histochemical method proved to be a powerful tool allowing to identify not only whole NPs while entering cells and moving into the cytoplasm, but also their remnants following lysosomal degradation and extrusion.
Source: European Journal of Histochemistry - Category: Biomedical Science Source Type: research