Aberrant expression of < em > CCDC69 < /em > in breast cancer and its clinicopathologic significance

Coiled-coil domain-containing protein 69 (CCDC69) is a novel gene and limited knowledge in known in breast cancer. In the present study, we aimed to explore the relationship betweenCCDC69 and breast cancer, demonstrate the clinicopathological significance and prognostic role ofCCDC69 in breast cancer, and analyze the possible mechanism ofCCDC69 affecting the prognosis of breast cancer. First, from GEO database, TIMER, GEPIA, and OncoLnc, we selectCCDC69 as the potential gene which closely involved in breast cancer progression. Next, by real-time PCR detection, the expression ofCCDC69 in breast cancer tissue was notably lower than that in normal breast tissues (p=0.0002). In addition, our immunohistochemistry (IHC) indicated that the positive expression rate ofCCDC69 in the triple-negative breast cancer (TNBC) was lower than that in the non-TNBC (p=0.0362), and it was negatively correlated with the expression of Ki67 (p=0.001). Further enrichment analysis ofCCDC69 and the similar genes performed on FunRich3.1.3 revealed that these genes were significantly associated with fat differentiation, and most of them were related to peroxisome proliferator-activated receptor (PPAR) signal pathway. Collectively, our findings suggest thatCCDC69 is down regulated in breast cancer tissue especially in TNBC which has higher malignant grade and poorer clinical prognosis.
Source: European Journal of Histochemistry - Category: Biomedical Science Authors: Source Type: research