Effects of mifepristone on adipocyte differentiation in mouse 3T3-L1 cells
CONCLUSIONS: Mifepristone alone is capable of inducing adipocyte differentiation in 3T3-L1 cells and adipogenesis in vivo. PPARγ plays a critical role in the mifepristone-induced adipocyte differentiation. Mifepristone-induced adipocytes are closer to the in situ adipocytes than those induced by the conventional protocol. The present study proposes a single treatment with mifepristone as a novel protocol to induce more physiologically relevant adipocytes in 3T3-L1 cells than the conventional protocol.PMID:38553665 | PMC:PMC10981365 | DOI:10.1186/s11658-024-00559-9 (Source: Cellular and Molecular Biology Letters)
Source: Cellular and Molecular Biology Letters - March 30, 2024 Category: Biochemistry Authors: Takeshi Hashimoto Katsuya Hirano Source Type: research
PANoptosis, an indicator of COVID-19 severity and outcomes
This study conducted a bioinformatics analysis of online single-cell RNA sequence (scRNA-seq) and bulk RNA-seq datasets to explore the potential of PANoptosis as an indicator of COVID-19 severity. The degree of PANoptosis in bronchoalveolar lavage fluid (BALF) and peripheral blood mononuclear cells (PBMC) indicated the severity of COVID-19. Single-cell transcriptomics identified pro-inflammatory monocytes as one of the primary sites of PANoptosis in COVID-19. The study subsequently demonstrated the immune and metabolic characteristics of this group of pro-inflammatory monocytes. In addition, the analysis illustrated that d...
Source: Briefings in Bioinformatics - March 30, 2024 Category: Bioinformatics Authors: Qingyuan Yang Wanmei Song Hanizaier Reheman Dan Wang Jieming Qu Yanan Li Source Type: research
Effects of mifepristone on adipocyte differentiation in mouse 3T3-L1 cells
CONCLUSIONS: Mifepristone alone is capable of inducing adipocyte differentiation in 3T3-L1 cells and adipogenesis in vivo. PPARγ plays a critical role in the mifepristone-induced adipocyte differentiation. Mifepristone-induced adipocytes are closer to the in situ adipocytes than those induced by the conventional protocol. The present study proposes a single treatment with mifepristone as a novel protocol to induce more physiologically relevant adipocytes in 3T3-L1 cells than the conventional protocol.PMID:38553665 | PMC:PMC10981365 | DOI:10.1186/s11658-024-00559-9 (Source: Mol Biol Cell)
Source: Mol Biol Cell - March 30, 2024 Category: Molecular Biology Authors: Takeshi Hashimoto Katsuya Hirano Source Type: research
Effects of mifepristone on adipocyte differentiation in mouse 3T3-L1 cells
CONCLUSIONS: Mifepristone alone is capable of inducing adipocyte differentiation in 3T3-L1 cells and adipogenesis in vivo. PPARγ plays a critical role in the mifepristone-induced adipocyte differentiation. Mifepristone-induced adipocytes are closer to the in situ adipocytes than those induced by the conventional protocol. The present study proposes a single treatment with mifepristone as a novel protocol to induce more physiologically relevant adipocytes in 3T3-L1 cells than the conventional protocol.PMID:38553665 | PMC:PMC10981365 | DOI:10.1186/s11658-024-00559-9 (Source: Cellular and Molecular Biology Letters)
Source: Cellular and Molecular Biology Letters - March 30, 2024 Category: Biochemistry Authors: Takeshi Hashimoto Katsuya Hirano Source Type: research
α2,6-sialic Acid on the Platelet Membrane Surface is a Novel Biomarker and Therapeutic Target in Children with Primary Immune Thrombocytopenia
CONCLUSION: The sialylation of platelet membrane glycoprotein is abnormally expressed in primary immune thrombocytopenia. The sialylation of platelet membrane glycoprotein may be involved in the occurrence and development of the disease. It is of great practical significance to diagnose and evaluate the therapeutic effect of the disease by detecting the sialylation of platelet membrane glycoprotein.PMID:38551414 (Source: Alternative Therapies in Health and Medicine)
Source: Alternative Therapies in Health and Medicine - March 29, 2024 Category: Complementary Medicine Authors: Jingxin Gong Zhaoyang Liu Wenjuan Wang Cuimin Zhu Chen Li Source Type: research
α2,6-sialic Acid on the Platelet Membrane Surface is a Novel Biomarker and Therapeutic Target in Children with Primary Immune Thrombocytopenia
CONCLUSION: The sialylation of platelet membrane glycoprotein is abnormally expressed in primary immune thrombocytopenia. The sialylation of platelet membrane glycoprotein may be involved in the occurrence and development of the disease. It is of great practical significance to diagnose and evaluate the therapeutic effect of the disease by detecting the sialylation of platelet membrane glycoprotein.PMID:38551414 (Source: Alternative Therapies in Health and Medicine)
Source: Alternative Therapies in Health and Medicine - March 29, 2024 Category: Complementary Medicine Authors: Jingxin Gong Zhaoyang Liu Wenjuan Wang Cuimin Zhu Chen Li Source Type: research
α2,6-sialic Acid on the Platelet Membrane Surface is a Novel Biomarker and Therapeutic Target in Children with Primary Immune Thrombocytopenia
CONCLUSION: The sialylation of platelet membrane glycoprotein is abnormally expressed in primary immune thrombocytopenia. The sialylation of platelet membrane glycoprotein may be involved in the occurrence and development of the disease. It is of great practical significance to diagnose and evaluate the therapeutic effect of the disease by detecting the sialylation of platelet membrane glycoprotein.PMID:38551414 (Source: Alternative Therapies in Health and Medicine)
Source: Alternative Therapies in Health and Medicine - March 29, 2024 Category: Complementary Medicine Authors: Jingxin Gong Zhaoyang Liu Wenjuan Wang Cuimin Zhu Chen Li Source Type: research
α2,6-sialic Acid on the Platelet Membrane Surface is a Novel Biomarker and Therapeutic Target in Children with Primary Immune Thrombocytopenia
CONCLUSION: The sialylation of platelet membrane glycoprotein is abnormally expressed in primary immune thrombocytopenia. The sialylation of platelet membrane glycoprotein may be involved in the occurrence and development of the disease. It is of great practical significance to diagnose and evaluate the therapeutic effect of the disease by detecting the sialylation of platelet membrane glycoprotein.PMID:38551414 (Source: Alternative Therapies in Health and Medicine)
Source: Alternative Therapies in Health and Medicine - March 29, 2024 Category: Complementary Medicine Authors: Jingxin Gong Zhaoyang Liu Wenjuan Wang Cuimin Zhu Chen Li Source Type: research
α2,6-sialic Acid on the Platelet Membrane Surface is a Novel Biomarker and Therapeutic Target in Children with Primary Immune Thrombocytopenia
CONCLUSION: The sialylation of platelet membrane glycoprotein is abnormally expressed in primary immune thrombocytopenia. The sialylation of platelet membrane glycoprotein may be involved in the occurrence and development of the disease. It is of great practical significance to diagnose and evaluate the therapeutic effect of the disease by detecting the sialylation of platelet membrane glycoprotein.PMID:38551414 (Source: Alternative Therapies in Health and Medicine)
Source: Alternative Therapies in Health and Medicine - March 29, 2024 Category: Complementary Medicine Authors: Jingxin Gong Zhaoyang Liu Wenjuan Wang Cuimin Zhu Chen Li Source Type: research
Molecules, Vol. 29, Pages 1546: Channel Expansion in the Ligand-Binding Domain of the Glucocorticoid Receptor Contributes to the Activity of Highly Potent Glucocorticoid Analogues
Shawn R. Campagna
Glucocorticoids (GCs) act through the glucocorticoid receptor (GR) and are commonly used as anti-inflammatory and immunosuppressant medications. Chronic GC use has been linked with unwanted complications such as steroid-induced diabetes mellitus (SIDM), although the mechanisms for these effects are not completely understood. Modification of six GC parent molecules with 2-mercaptobenzothiazole resulted in consistently less promoter activity in transcriptional activation assays using a 3xGRE reporter construct while constantly reducing inflammatory pathway activity. The most selective candidate, DX1, ...
Source: Molecules - March 29, 2024 Category: Chemistry Authors: Wesley B. Seaton Susan J. Burke Alexander R. Fisch William A. Schilletter Mary Grace A. Beck Gabrielle A. Cassagne Innocence Harvey Molly S. Fontenot J. Jason Collier Shawn R. Campagna Tags: Article Source Type: research
Controlled Release of Drugs from Extracellular Matrix-Derived Peptide-Based Nanovesicles through Tailored Noncovalent Interactions
This study provides important insights into the drug delivery potential of ECnVs and offers useful information for future development of ECnV-based drug delivery systems for the treatment of various diseases.PMID:38546162 | DOI:10.1021/acs.biomac.3c01361 (Source: Biomacromolecules)
Source: Biomacromolecules - March 28, 2024 Category: Biochemistry Authors: Haofu Huang Jeongmin Hwang Sudha Anilkumar Kristi L Kiick Source Type: research
Efficacy and safety of daratumumab plus bortezomib and dexamethasone in newly diagnosed Mayo 2004 stage IIIA or IIIB light-chain amyloidosis: a prospective phase II study
Haematologica. 2024 Mar 28. doi: 10.3324/haematol.2024.285145. Online ahead of print.ABSTRACTNot available.PMID:38546676 | DOI:10.3324/haematol.2024.285145 (Source: Haematologica)
Source: Haematologica - March 28, 2024 Category: Hematology Authors: Kai-Ni Shen Ya-Juan Gao Long Chang Lu Zhang Xin-Xin Cao Zhuang Tian Yi-Ning Wang Dao-Bin Zhou Jian Li Source Type: research
Controlled Release of Drugs from Extracellular Matrix-Derived Peptide-Based Nanovesicles through Tailored Noncovalent Interactions
This study provides important insights into the drug delivery potential of ECnVs and offers useful information for future development of ECnV-based drug delivery systems for the treatment of various diseases.PMID:38546162 | DOI:10.1021/acs.biomac.3c01361 (Source: Biomacromolecules)
Source: Biomacromolecules - March 28, 2024 Category: Biochemistry Authors: Haofu Huang Jeongmin Hwang Sudha Anilkumar Kristi L Kiick Source Type: research
Biomaterial-mediated intracellular control of macrophages for cell therapy in pro-inflammatory and pro-fibrotic conditions
We present a tunable biomaterial-based strategy to control macrophages intracellularly via small molecule-releasing microparticles. Poly(lactic-co-glycolic acid) microparticles encapsulating the anti-inflammatory and anti-fibrotic drug dexamethasone were administered to macrophages in vitro, with uptake rates controlled by different loading regimes. Microparticle dose and dexamethasone content directly affected macrophage phenotype and phagocytic capacity, independent of particle content per cell, leading to an overall pro-reparative, anti-inflammatory, anti-fibrotic phenotype with increased phagocytic and ECM degrading fu...
Source: Biomaterials - March 28, 2024 Category: Materials Science Authors: Tina Tylek Joanna Wong Andrew E Vaughan Kara L Spiller Source Type: research
Efficacy and safety of daratumumab plus bortezomib and dexamethasone in newly diagnosed Mayo 2004 stage IIIA or IIIB light-chain amyloidosis: a prospective phase II study
Haematologica. 2024 Mar 28. doi: 10.3324/haematol.2024.285145. Online ahead of print.ABSTRACTNot available.PMID:38546676 | DOI:10.3324/haematol.2024.285145 (Source: Haematologica)
Source: Haematologica - March 28, 2024 Category: Hematology Authors: Kai-Ni Shen Ya-Juan Gao Long Chang Lu Zhang Xin-Xin Cao Zhuang Tian Yi-Ning Wang Dao-Bin Zhou Jian Li Source Type: research
