Lotus seedpod-inspired hydrogels as an all-in-one platform for culture and delivery of stem cell spheroids.
Abstract 3D culture of stem cells can improve therapeutic effects. However, there is limited research on how to deliver cultured stem cell spheroids to the desired target. Here, we developed lotus seedpod-inspired hydrogel (LoSH) containing microwells for culture and delivery of stem cell spheroids. Human adipose-derived stem cells (hADSCs) inside the square microwells (200 or 400 μm in width with various depths) spontaneously formed spheroids with high viability (94.08 ± 1.56%), and fibronectins conjugated to the hydrogel successfully gripped the spheroids, similar to the funiculus gripping seeds ...
Source: Biomaterials - September 30, 2019 Category: Materials Science Authors: Kim SJ, Park J, Kim EM, Choi JJ, Kim HN, Chin IL, Choi YS, Moon SH, Shin H Tags: Biomaterials Source Type: research

Iron chelated melanin-like nanoparticles for tumor-associated macrophage repolarization and cancer therapy.
Abstract Tumor-associated macrophages (TAMs) are abundant in many cancers, and predominately display an immunosuppressive M2-like function that fosters tumor progression and promotes malignant metastasis. Current TAMs repolarization strategies mainly focused on harnessing the direct cancer cell killing property of M1-like macrophages repolarized from TAMs. However, the latent role of M1-like macrophages as professional antigen-presenting cells (APCs) also needs to be explored. Here, iron chelated melanin-like nanoparticles (Fe@PDA-PEG) were developed for M2-to-M1 TAMs repolarization and photothermal therapy (PTT) ...
Source: Biomaterials - September 27, 2019 Category: Materials Science Authors: Rong L, Zhang Y, Li WS, Su Z, Fadhil JI, Zhang C Tags: Biomaterials Source Type: research

Development of DNA-anchored assembly of small extracellular vesicle for efficient antigen delivery to antigen presenting cells.
Abstract Tumor-cell derived small extracellular vesicle (sEV) combined with immunostimulatory adjuvants may serve as a promising tumor vaccine through the induction of the cytotoxic T cell response. To achieve an efficient immune response, the prolonged tissue residence after intradermal injection followed by the sustained and efficient delivery of tumor-cell derived sEV combined with adjuvants to antigen-presenting cells (APCs) is a promising strategy. In the present study, we constructed a DNA-anchored sEV superstructure in which tumor-cell derived sEVs were assembled with each other to achieve prolonged tissue ...
Source: Biomaterials - September 25, 2019 Category: Materials Science Authors: Matsumoto A, Takahashi Y, Ariizumi R, Nishikawa M, Takakura Y Tags: Biomaterials Source Type: research

3D bioengineered tissue model of the large intestine to study inflammatory bowel disease.
Abstract An in vitro model of intestinal epithelium with an immune component was bioengineered to mimic immunologic responses seen in inflammatory bowel disease. While intestinal immune phenomena can be modeled in transwells and 2D culture systems, 3D tissue models improve physiological relevance by providing a 3D substrate which enable migration of macrophages towards the epithelium. An intestinal epithelial layer comprised of non-transformed human colon organoid cells and a subepithelial layer laden with monocyte-derived macrophages was bioengineered to mimic native intestinal mucosa cell organization using spon...
Source: Biomaterials - September 25, 2019 Category: Materials Science Authors: Roh TT, Chen Y, Paul HT, Guo C, Kaplan DL Tags: Biomaterials Source Type: research

Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts.
In this study, we aimed to develop novel injectable MSC-delivery microgel systems possessing high anti-oxidant activities. Specifically, we encapsulated MSCs in graphene oxide (GO)/alginate composite microgels by electrospraying. To further enhance the anti-oxidizing activities of the gels, we developed reduced MSC-embedded GO/alginate microgels (i.e., r(GO/alginate)), which have the potential to protect MSCs from the abovementioned harsh environments within MI tissues. Our in vitro studies demonstrated that the MSCs encapsulated in the r(GO/alginate) microgels showed increased viability under oxidative stress conditions w...
Source: Biomaterials - September 24, 2019 Category: Materials Science Authors: Choe G, Kim SW, Park J, Park J, Kim S, Kim YS, Ahn Y, Jung DW, Williams DR, Lee JY Tags: Biomaterials Source Type: research

Selenoxide elimination manipulate the oxidative stress to improve the antitumor efficacy.
Abstract Selenoxide elimination reaction has been widely used in the field of organic synthesis. However, few studies have been conducted to apply this reaction in biodegradable nanomedicine. In this work, the selenoxide elimination reaction was used for cancer treatment via producing excess cellular reactive oxygen species (ROS) for the first time. The β-seleno diesters and porphyrin derivates containing nanoparticle could be responsive to the intracellular ROS and produce acrylates through the elimination reaction. The acrylates would further deplete intracellular GSH in tumor cells and finally improved the...
Source: Biomaterials - September 24, 2019 Category: Materials Science Authors: Sun C, Wang L, Xianyu B, Li T, Gao S, Xu H Tags: Biomaterials Source Type: research

Near-infrared boosted ROS responsive siRNA delivery and cancer therapy with sequentially peeled upconversion nano-onions.
Abstract RNA interference (RNAi) therapy has become an appealing approach for cancer treatment, while the specificity and efficiency of controlled small interference RNA (siRNA) release remain challenging due to the heterogeneity of tumor environment. Herein, upconversion nano-onions (UCNOs) with stacked polymer coating layers are constructed to decompose sequentially in response to extracellular environment and NIR stimulation. The UCNOs (UCNPs-PEIRB-PEISeSe/siRNA-R8-HA) are composed of upconversion nanoparticles (UCNPs) core functionalized with inner coating layer of photosensitizer rose bengal (RB) conjugated P...
Source: Biomaterials - September 20, 2019 Category: Materials Science Authors: He Y, Guo S, Wu L, Chen P, Wang L, Liu Y, Ju H Tags: Biomaterials Source Type: research

Stem cell derived exosomes: microRNA therapy for age-related musculoskeletal disorders.
g H Abstract Age-associated musculoskeletal disorders (MSDs) have been historically overlooked by mainstream biopharmaceutical researchers. However, it has now been recognized that stem and progenitor cells confer innate healing capacity for the musculoskeletal system. Current evidence indicates that exosomes are particularly important in this process as they can mediate sequential and reciprocal interactions between cells to initiate and enhance healing. The present review focuses on stem cells (SCs) derived exosomes as a regenerative therapy for treatment of musculoskeletal disorders. We discuss mechanisms invol...
Source: Biomaterials - September 17, 2019 Category: Materials Science Authors: Yao X, Wei W, Wang X, Chenglin L, Björklund M, Ouyang H Tags: Biomaterials Source Type: research

Nanotherapeutics interfere with cellular redox homeostasis for highly improved photodynamic therapy.
Abstract Redox homeostasis inside malignant cells is a defense mechanism against the reactive oxygen species (ROS)-induced therapy means, but little importance has been paid to this innate barrier. The present study intends to make cancer cells more sensitive to the ROS-induced therapy by disturbing cellular redox homeostasis. To verify this concept, a porous metal-organic framework (MOF) serves not only as the photodynamic therapy (PDT) agent but also as the carrier to transport alkaloid piperlongumine (PL), a thioredoxin reductase (TrxR) inhibitor used to disturb cellular redox homeostasis. The PL-loaded MOF was...
Source: Biomaterials - September 17, 2019 Category: Materials Science Authors: Cheng Q, Yu W, Ye J, Liu M, Liu W, Zhang C, Zhang C, Feng J, Zhang XZ Tags: Biomaterials Source Type: research

Epigenetics-inspired photosensitizer modification for plasma membrane-targeted photodynamic tumor therapy.
Abstract In recent years, epigenetics has attracted great attentions in the field of biomedicine, which is used to denote the heritable changes in gene expression without any variation in DNA sequence, including DNA methylation, histone modification and so on. Inspired by it, a simple and versatile amino acids modification strategy is proposed in this paper to regulate the subcellular distribution of photosensitizer for plasma membrane targeted photodynamic therapy (PDT). Particularly, the plasma membrane anchoring ability and photo toxicity of the photosensitizer against different cell lines could be effectively ...
Source: Biomaterials - September 16, 2019 Category: Materials Science Authors: Cheng H, Fan GL, Fan JH, Yuan P, Deng FA, Qiu XZ, Yu XY, Li SY Tags: Biomaterials Source Type: research

Corrigendum to "A carrier-free multiplexed gene editing system applicable for suspension cells" [Biomaterials 217 (2019) 119298].
Corrigendum to "A carrier-free multiplexed gene editing system applicable for suspension cells" [Biomaterials 217 (2019) 119298]. Biomaterials. 2019 Sep 14;223:119487 Authors: Ju A, Lee S, Lee YE, Han KC, Kim JC, Shin SC, Park HJ, EunKyeong Kim E, Hong S, Jang M PMID: 31526951 [PubMed - as supplied by publisher] (Source: Biomaterials)
Source: Biomaterials - September 14, 2019 Category: Materials Science Authors: Ju A, Lee S, Lee YE, Han KC, Kim JC, Shin SC, Park HJ, EunKyeong Kim E, Hong S, Jang M Tags: Biomaterials Source Type: research

Triggered ferroptotic polymer micelles for reversing multidrug resistance to chemotherapy.
Abstract Drug-tolerant persister cancer cells (PCCs) play an important role in the development of multidrug resistance (MDR) to anti-cancer drugs. This is due to the strong link between PCCs formation and epithelial-mesenchymal transition (EMT), as well as the low numbers of PCCs. In addition, PCC removal by traditional cytotoxic agents is poor due to the intrinsic high MDR activity in these cells. As a novel programmed cell death pathway, ferroptosis shows high potency to eliminate cells at the EMT state via manipulating intracellular redox homeostasis. The aim of this work was to utilize triggered ferroptotic po...
Source: Biomaterials - September 7, 2019 Category: Materials Science Authors: Gao M, Deng J, Liu F, Fan A, Wang Y, Wu H, Ding D, Kong D, Wang Z, Peer D, Zhao Y Tags: Biomaterials Source Type: research

Remote eradication of biofilm on titanium implant via near-infrared light triggered photothermal/photodynamic therapy strategy.
Abstract Biofilm formation is a main challenge in treatment of bone-implant-associated infections, resulting in tolerance to immune system and antibiotics. However, smart non-surgical or non-invasive treatment methods of combating established biofilm on an implant have been less reported. Herein, a therapeutic system consisting of mesoporous polydopamine nanoparticles (MPDA) to combat biofilm is reported for the first time. We develop a synergistic photothermal/photodynamic therapy (PTT/PDT) strategy aiming for biofilms eradication on titanium (Ti) implant, which is integrated with MPDA loading with photosensitize...
Source: Biomaterials - September 6, 2019 Category: Materials Science Authors: Yuan Z, Tao B, He Y, Mu C, Liu G, Zhang J, Liao Q, Liu P, Cai K Tags: Biomaterials Source Type: research

The potentiated checkpoint blockade immunotherapy by ROS-responsive nanocarrier-mediated cascade chemo-photodynamic therapy.
Abstract Checkpoint inhibitors, such as anti-PD-1/PD-L1 antibodies, have been proven as a promising type of immunotherapy in a number of cancers, but the relatively low response rates limit their scope of clinical application. Here, we report the use of cascade chemo-photodynamic therapy (chemo-PDT) with reactive oxygen species (ROS)-sensitive lipid-polymer hybrid nanoparticles TKHNP-C/D to potentiate the antitumor efficacy of anti-PD-L1 antibody (aPD-L1). Under light irradiation, TKHNP-C/D not only induced photodynamic therapy (PDT) but also boosted intracellular DOX release via the rapid degradation of its hydro...
Source: Biomaterials - September 5, 2019 Category: Materials Science Authors: Hu L, Cao Z, Ma L, Liu Z, Liao G, Wang J, Shen S, Li D, Yang X Tags: Biomaterials Source Type: research

Functional extracellular vesicles engineered with lipid-grafted hyaluronic acid effectively reverse cancer drug resistance.
Abstract Multidrug resistance (MDR) is a key issue accounting for ineffectiveness of cancer chemotherapy. Numerous multifunctional nanocarriers have been developed to increase drug delivery efficacy and inhibit drug efflux for overcoming cancer drug resistance. However, limited success has been achieved in clinic because of nanocarriers' complicated multi-step fabrication procedures and their undesired side toxicity as well as potential immunogenicity. Here, hyaluronic acid (HA) functionalized extracellular vesicles (EVs) are generated as natural vehicles to efficiently deliver doxorubicin (DOX) and reverse MDR. T...
Source: Biomaterials - September 5, 2019 Category: Materials Science Authors: Liu J, Ye Z, Xiang M, Chang B, Cui J, Ji T, Zhao L, Li Q, Deng Y, Xu L, Wang G, Wang L, Wang Z Tags: Biomaterials Source Type: research

Integration of cascade delivery and tumor hypoxia modulating capacities in core-releasable satellite nanovehicles to enhance tumor chemotherapy.
Abstract Drug nanovehicles owning tumor microenvironment responsive and modulating capacities are highly demanding for effective tumor chemotherapy but still lack of exploration. Here, a kind of core-releasable satellite nanovehicles was rational constructed, which is composed of polydopamine (PDA) cores as photothermal agents and the carrier for small satellite nanoparticles (NPs) and drugs, G5Au NPs as the drug-loading satellites for deep tumor drug delivery and as catalase-like agents for relieving tumor hypoxia, doxorubicin (DOX) as the model chemotherapeutic drug loaded by both PDA and G5Au NPs, and polyethyl...
Source: Biomaterials - September 5, 2019 Category: Materials Science Authors: Wang J, Wang X, Lu SY, Hu J, Zhang W, Xu L, Gu D, Yang W, Tang W, Liu F, Cao Y, Liu H Tags: Biomaterials Source Type: research

Ta ion implanted nanoridge-platform for enhanced vascular responses.
In this study, we introduce a target-ion-induced plasma sputtering (TIPS) technique to fabricate a platform with a favorable endothelial environment. This technique enables the simple single-step production of a Ta-implanted nanoridged surface on a stent with a complex 3D geometry that shows a clear tendency to become oriented parallel to the direction of blood flow. Moreover, the nanoridges developed show good structural integrity and mechanical stability, resulting in apparently stable morphologies under high strain rates. In vitro cellular responses to the Co-Cr, such as endothelialization, platelet activation, and bloo...
Source: Biomaterials - September 5, 2019 Category: Materials Science Authors: Jang TS, Lee JH, Kim S, Park C, Song J, Jae HJ, Kim HE, Chung JW, Jung HD Tags: Biomaterials Source Type: research

Magnetic nanocomposite hydrogels and static magnetic field stimulate the osteoblastic and vasculogenic profile of adipose-derived cells.
Abstract Exposure of cells to externally applied magnetic fields or to scaffolding materials with intrinsic magnetic properties (magnetic actuation) can regulate several biological responses. Here, we generated novel magnetized nanocomposite hydrogels by incorporation of magnetic nanoparticles (MNPs) into polyethylene glycol (PEG)-based hydrogels containing cells from the stromal vascular fraction (SVF) of human adipose tissue. We then investigated the effects of an external Static Magnetic Field (SMF) on the stimulation of osteoblastic and vasculogenic properties of the constructs, with MNPs or SMF alone used as ...
Source: Biomaterials - September 5, 2019 Category: Materials Science Authors: Filippi M, Dasen B, Guerrero J, Garello F, Isu G, Born G, Ehrbar M, Martin I, Scherberich A Tags: Biomaterials Source Type: research

Hydrogen gas improves photothermal therapy of tumor and restrains the relapse of distant dormant tumor.
Abstract Inflammation during photothermal therapy (PTT) of tumor usually results in adverse consequences. Here, a biomembrane camouflaged nanomedicine (mPDAB) containing polydopamine and ammonia borane was designed to enhance PTT efficacy and mitigate inflammation. Polydopamine, a biocompatible photothermal agent, can effectively convert light into heat for PTT. Ammonia borane was linked to the surface of polydopamine through the interaction of hydrogen bonding, which could destroy redox homoeostasis in tumor cells and reduce inflammation by H2 release in tumor microenvironment. Owing to the same origin of outer b...
Source: Biomaterials - September 3, 2019 Category: Materials Science Authors: Zhang C, Zheng DW, Li CX, Zou MZ, Yu WY, Liu MD, Peng SY, Zhong ZL, Zhang XZ Tags: Biomaterials Source Type: research

Hyaluronic acid-targeted and pH-responsive drug delivery system based on metal-organic frameworks for efficient antitumor therapy.
Abstract Drug delivery systems (DDSs) have emerged to help delivering the required cargo into the region of the tumor, achieving the objectives of extenuating the potential damage to the body and improving the therapeutic effectiveness. Here, we developed a one-pot process for encapsulating the unstable and hydrophobic d-α-Tocopherol succinate (α-TOS) in zeolitic imidazolate framework-8 (ZIF-8) compounds (defined as α-TOS@ZIF-8) and subsequently coated with a hyaluronic acid (HA) shell to form the HA/α-TOS@ZIF-8 nanoplatform. Of particular note was when the concentration of α-TOS is l...
Source: Biomaterials - September 3, 2019 Category: Materials Science Authors: Sun Q, Bi H, Wang Z, Li C, Wang X, Xu J, Zhu H, Zhao R, He F, Gai S, Yang P Tags: Biomaterials Source Type: research

Porphyrin-based covalent organic framework nanoparticles for photoacoustic imaging-guided photodynamic and photothermal combination cancer therapy.
Abstract Combination of photodynamic therapy (PDT) and photothermal therapy (PTT) generally requires different components to build a composite irradiated with different excitation lights. One component photoactive agent for enhanced combination of PDT and PTT under the excitation of a single wavelength light source is more urgent in tumor phototherapy via adjusting spatial arrangement of photoactive units. Herein, porphyrin-based covalent organic framework nanoparticles (COF-366 NPs) were synthesized to control the orderly spatial arrangement of the photoactive building units and firstly used for antitumor therapy...
Source: Biomaterials - August 30, 2019 Category: Materials Science Authors: Wang D, Zhang Z, Lin L, Liu F, Wang Y, Guo Z, Li Y, Tian H, Chen X Tags: Biomaterials Source Type: research

Renal-clearable ultrasmall covalent organic framework nanodots as photodynamic agents for effective cancer therapy.
Abstract Covalent organic frameworks (COFs) and their derivatives represent an emerging class of crystalline porous materials with broad potential applications. However, the biomedical applications of them were limited by the large size, low dispersivity, poor bioavailability within cells and metabolic problems. Herein, renal-clearable ultrasmall COF nanodots have been synthesized and utilized as efficient cancer therapy agents. A simple liquid exfoliation strategy was used to prepare COF nanodots. After polyethylene glycol (PEG) conjugation, the PEG coated COF nanodots (COF nanodots-PEG) showed improved physiolog...
Source: Biomaterials - August 30, 2019 Category: Materials Science Authors: Zhang Y, Zhang L, Wang Z, Wang F, Kang L, Cao F, Dong K, Ren J, Qu X Tags: Biomaterials Source Type: research

Simultaneous nano- and microscale structural control of injectable hydrogels via the assembly of nanofibrous protein microparticles for tissue regeneration.
Abstract Injectable hydrogels are advantageous as tissue regeneration scaffolds, as they can be delivered through a minimally invasive injection and seamlessly integrate with the target tissues. However, an important shortcoming of current injectable hydrogels is the lack of simultaneous control over their micro- and nanoscale structures. In this article, the authors report a strategy for developing injectable hydrogels that integrate a fibrous nanostructure and porous microstructure. The hydrogels are prepared by using novel nanofibrous microparticles as the building blocks. The protein based nanofibrous micropar...
Source: Biomaterials - August 29, 2019 Category: Materials Science Authors: Hou S, Niu X, Li L, Zhou J, Qian Z, Yao D, Yang F, Ma PX, Fan Y Tags: Biomaterials Source Type: research

Liver-targeted Nano-MitoPBN normalizes glucose metabolism by improving mitochondrial redox balance.
Abstract Recent advances in Nanomedicine provide promising disease treatment through improved drug delivery efficiency, but clinical applications have encountered difficulties, largely due to the majority of injected nanoparticle is sequestered in liver. In contrast, liver cells seem to be a perfect target for nanoparticles. Here we generated a new formula of liposome encapsulated Nano-MitoPBN as a liver mitochondrial-targeting free radical scavenger. We found that Nano-MitoPBN mainly accumulated in hepatocytes and scavenged hepatic mitochondrial superoxide/hydrogen peroxide generated from mono-electron leak of el...
Source: Biomaterials - August 26, 2019 Category: Materials Science Authors: Wu M, Liao L, Jiang L, Zhang C, Gao H, Qiao L, Liu S, Shi D Tags: Biomaterials Source Type: research

Chiral geometry regulates stem cell fate and activity.
Abstract Geometry sensing of cells inevitably involves cytoskeletal remodeling and the activation of biochemical signaling, which control multiple aspects of cell behaviors, such as proliferation, differentiation and migration. A variety of size-, shape- and geometry-dependent cell behaviors have been revealed, but the role of geometric chirality in regulating cellular behaviors and the underlying biophysical mechanisms remain elusive. Here, we report an intriguing mechanotransduction of stem cells on chiral geometries that human mesenchymal stem cells (hMSCs) prefer to migrate towards dextral geometry with nearly...
Source: Biomaterials - August 26, 2019 Category: Materials Science Authors: Dong L, Gong J, Wang Y, He J, You D, Zhou Y, Li Q, Liu Y, Cheng K, Qian J, Weng W, Wang H, Yu M Tags: Biomaterials Source Type: research

Influence of hyaluronic acid modification on CD44 binding towards the design of hydrogel biomaterials.
ck JA Abstract Hyaluronic acid (HA) is a linear polysaccharide of d-glucuronic acid and N-acetyl-d-glucosamine that is native to many tissues and interacts with cells via cell-surface receptors (e.g., CD44). HA has been extensively explored as a chemically-modified macromer for crosslinking into biomaterials, such as hydrogels and macroporous scaffolds. However, the influence of the extent and type of HA modification on its binding to CD44 is not well understood or quantified. To address this, we modified HA at either the carboxylic acid or the primary alcohol with various chemical groups (e.g., norbornenes, metha...
Source: Biomaterials - August 23, 2019 Category: Materials Science Authors: Kwon MY, Wang C, Galarraga JH, Puré E, Han L, Burdick JA Tags: Biomaterials Source Type: research

Bio-inspired swellable hydrogel-forming double-layered adhesive microneedle protein patch for regenerative internal/external surgical closure.
Abstract Significant tissue damage, scarring, and an intense inflammatory response remain the greatest concerns for conventional wound closure options, including sutures and staples. In particular, wound closure in internal organs poses major clinical challenges due to air/fluid leakage, local ischemia, and subsequent impairment of healing. Herein, to overcome these limitations, inspired by endoparasites that swell their proboscis to anchor to host's intestines, we developed a hydrogel-forming double-layered adhesive microneedle (MN) patch consisting of a swellable mussel adhesive protein (MAP)-based shell and a n...
Source: Biomaterials - August 21, 2019 Category: Materials Science Authors: Jeon EY, Lee J, Kim BJ, Joo KI, Kim KH, Lim G, Cha HJ Tags: Biomaterials Source Type: research

Antigen-specific immune decoys intercept and exhaust autoimmunity to prevent disease.
Abstract Relapsing-remitting patterns of many autoimmune diseases such as multiple sclerosis (MS) are perpetuated by a recurring circuit of adaptive immune cells that amplify in secondary lymphoid organs (SLOs) and traffic to compartments where antigen is abundant to elicit damage. Some of the most effective immunotherapies impede the migration of immune cells through this circuit, however, broadly suppressing immune cell migration can introduce life-threatening risks for patients. We developed antigen-specific immune decoys (ASIDs) to mimic tissues targeted in autoimmunity and selectively intercept autoimmune cel...
Source: Biomaterials - August 19, 2019 Category: Materials Science Authors: Griffin JD, Song JY, Huang A, Sedlacek AR, Flannagan KL, Berkland CJ Tags: Biomaterials Source Type: research

Synthetic probes for in vitro purification and in vivo tracking of hepatocytes derived from human pluripotent stem cells.
Abstract Hepatocytes derived from human pluripotent stem cells (hPSCs) are promising candidates for cell therapy and drug discovery. However, it remains challenging to efficiently purify hepatocytes from undesired cell types after differentiation and to accurately monitor grafted cells after transplantation. Indocyanine Green (ICG), an FDA-approved, near-infrared (NIR) dye, has been used for various clinical purposes and is exclusively taken up by hepatocytes. However, ICG has a long emission wavelength (λem > 800 nm) that is beyond the detection range of fluorescence-activated cell sorting (FACS) ...
Source: Biomaterials - August 14, 2019 Category: Materials Science Authors: Park JY, Han J, Jung HS, Lee G, Kim HJ, Cho GS, Park HJ, Han C, Kim JS, Kim JH Tags: Biomaterials Source Type: research

3D printed HUVECs/MSCs cocultures impact cellular interactions and angiogenesis depending on cell-cell distance.
Abstract Vascularization is a crucial process during the growth and development of bone 1, yet it remains one of the main challenges in the reconstruction of large bone defects. The use of in vitro coculture of human umbilical vein endothelial cells (HUVECs) and human mesenchymal stem cells (hMSCs) has been one of the most explored options. Both cell types secrete specific growth factors that are mutually beneficial, and studies suggested that cell-cell communication and paracrine secretion could be affected by a number of factors. However, little is known about the effect of cell patterning and the distance betwe...
Source: Biomaterials - August 14, 2019 Category: Materials Science Authors: Piard C, Jeyaram A, Liu Y, Caccamese J, Jay SM, Chen Y, Fisher J Tags: Biomaterials Source Type: research

Selenium nanoparticles as new strategy to potentiate γδ T cell anti-tumor cytotoxicity through upregulation of tubulin-α acetylation.
In conclusion, SeNPs could significantly potentiate anti-tumor cytotoxicity of Vγ9Vδ2 T cells, and both cytotoxicity related molecules and tubulin acetylation were involved in fine-tuning γδ T cell toxicity against cancer cells. Our present work demonstrated a new strategy for further enhancing anti-tumor cytotoxicity of human Vγ9Vδ2 T cells by using SeNPs-based nanotechnology, not gene modification, implicating SeNPs-based nanotechnology had a promising clinical perspective in the γδ T cell immunotherapy for malignant tumors. PMID: 31442884 [PubMed - as supplied by ...
Source: Biomaterials - August 13, 2019 Category: Materials Science Authors: Hu Y, Liu T, Li J, Mai F, Li J, Chen Y, Jing Y, Dong X, Lin L, He J, Xu Y, Shan C, Hao J, Yin Z, Chen T, Wu Y Tags: Biomaterials Source Type: research

Photothermal-pH-hypoxia responsive multifunctional nanoplatform for cancer photo-chemo therapy with negligible skin phototoxicity.
Abstract Highly specific and effective cancer phototherapy remains as a great challenge. Herein, a smart nanoplatform (TENAB NP) sequentially responsive to light, low pH and hypoxia is demonstrated for multi-mode imaging guided synergistic cancer therapy with negligible skin phototoxicity. Upon 808-nm laser irradiation, TENAB NPs can generate hyperthermia to melt the phase change material (PCM-LASA) coat and thereafter release chemo-drug tirapazamine (TPZ). Meanwhile, under acidic pH, photosensitizer ENAB would turn "off" its charge-transfer state, generating prominent 1O2 for photodynamic therapy (PDT) ...
Source: Biomaterials - August 12, 2019 Category: Materials Science Authors: Chen D, Tang Y, Zhu J, Zhang J, Song X, Wang W, Shao J, Huang W, Chen P, Dong X Tags: Biomaterials Source Type: research

Bismuth embedded silica nanoparticles loaded with autophagy suppressant to promote photothermal therapy.
This study demonstrates a potential new PTT platform with superior therapeutic efficacy. PMID: 31421315 [PubMed - as supplied by publisher] (Source: Biomaterials)
Source: Biomaterials - August 9, 2019 Category: Materials Science Authors: Chen T, Cen D, Ren Z, Wang Y, Cai X, Huang J, Di Silvio L, Li X, Han G Tags: Biomaterials Source Type: research

Multifunctional nanoparticles for genetic engineering and bioimaging of natural killer (NK) cell therapeutics.
Abstract Recently, natural killer (NK)-based immunotherapy has attracted attention as a next-generation cell-based cancer treatment strategy due to its mild side effects and excellent therapeutic efficacy. Here, we describe multifunctional nanoparticles (MF-NPs) capable of genetically manipulating NK cells and tracking them in vivo through non-invasive magnetic resonance (MR) and fluorescence optical imaging. The MF-NPs were synthesized with a core-shell structure by conjugation of a cationic polymer labeled with a near-infrared (NIR) fluorescent molecule, with the aid of a polydopamine (PDA) coating layer. When a...
Source: Biomaterials - August 9, 2019 Category: Materials Science Authors: Kim KS, Han JH, Park JH, Kim HK, Choi SH, Kim GR, Song H, An HJ, Han DK, Park W, Park KS Tags: Biomaterials Source Type: research

Engineered skeletal muscles for disease modeling and drug discovery.
Abstract Skeletal muscle is the largest organ of human body with several important roles in everyday movement and metabolic homeostasis. The limited ability of small animal models of muscle disease to accurately predict drug efficacy and toxicity in humans has prompted the development in vitro models of human skeletal muscle that fatefully recapitulate cell and tissue level functions and drug responses. We first review methods for development of three-dimensional engineered muscle tissues and organ-on-a-chip microphysiological systems and discuss their potential utility in drug discovery research and development o...
Source: Biomaterials - August 8, 2019 Category: Materials Science Authors: Wang J, Khodabukus A, Rao L, Vandusen K, Abutaleb N, Bursac N Tags: Biomaterials Source Type: research

Co-delivery of immunomodulators in biodegradable nanoparticles improves therapeutic efficacy of cancer vaccines.
In this study, the potential of intratumoral immune modulation with poly (I:C), Resiquimod (R848) and CCL20 (MIP3α) was explored. Biodegradable polymeric nanoparticles were used as delivery vehicles for slow and sustained release of these drugs in the tumor area and were combined with specific immunotherapy based on therapeutic peptide vaccination in two aggressive murine carcinoma and lymphoma tumor models. Whereas nanoparticle delivery of poly (I:C) or R848 improved therapeutic efficacy, the combination with MIP3α remarkably potentiated the cancer vaccine antitumor effects. The long-term survival increased to...
Source: Biomaterials - August 7, 2019 Category: Materials Science Authors: Da Silva CG, Camps MGM, Li TMWY, Chan AB, Ossendorp F, Cruz LJ Tags: Biomaterials Source Type: research

The design of a thermoresponsive surface for the continuous culture of human pluripotent stem cells.
Abstract Commonly, stem cell culture is based on batch-type culture, which is laborious and expensive. We continuously cultured human pluripotent stem cells (hPSCs) on thermoresponsive dish surfaces, where hPSCs were partially detached on the same thermoresponsive dish by decreasing the temperature of the thermoresponsive dish to be below the lower critical solution temperature for only 30 min. Then, the remaining cells were continuously cultured in fresh culture medium, and the detached stem cells were harvested in the exchanged culture medium. hPSCs were continuously cultured for ten cycles on the thermorespon...
Source: Biomaterials - August 7, 2019 Category: Materials Science Authors: Sung TC, Yang JS, Yeh CC, Liu YC, Jiang YP, Lu MW, Ling QD, Kumar SS, Chang Y, Umezawa A, Chen H, Higuchi A Tags: Biomaterials Source Type: research

Laser-triggered polymeric lipoproteins for precision tumor penetrating theranostics.
Abstract Natural particles ranging from various cell membranes to nascent proteins are highly optimized for their specific functions in vivo and possess features that are desired in drug delivery carriers. However, the current endeavor in research on bioparticles is still seeking the appropriate strategy to shield multiple agents and circumvent biological hurdles. These issues have propelled the advancement of lipid-polymer hybrid nanocarriers, which could be employed as drug reservoirs and strive to meet these expectations. We thereby proposed functionalized biopeptide-lipid hybrid particles, which were applied t...
Source: Biomaterials - August 6, 2019 Category: Materials Science Authors: Wang R, Zhang C, Li J, Huang J, Opoku-Damoah Y, Sun B, Zhou J, Di L, Ding Y Tags: Biomaterials Source Type: research

Engineering "cell-particle hybrids" of pancreatic islets and bioadhesive FK506-loaded polymeric microspheres for local immunomodulation in xenogeneic islet transplantation.
This study presents a promising platform that facilitates the translation of local immunomodulation towards an effective strategy with improved safety profiles for treating type I diabetes. PMID: 31419652 [PubMed - as supplied by publisher] (Source: Biomaterials)
Source: Biomaterials - August 6, 2019 Category: Materials Science Authors: Nguyen TT, Pham TT, Nguyen HT, Nepal MR, Phung CD, You Z, Katila N, Pun NT, Jeong TC, Choi DY, Park PH, Yong CS, Kim JO, Yook S, Jeong JH Tags: Biomaterials Source Type: research

A multifunctional lipid that forms contrast-agent liposomes with dual-control release capabilities for precise MRI-guided drug delivery.
Abstract Monitoring of nanoparticle-based therapy in vivo and controlled drug release are urgently needed for the precise treatment of disease. We have synthesized a multifunctional Gd-DTPA-ONB (GDO) lipid by introducing the Gd-DTPA contrast agent moiety into an o-nitro-benzyl ester lipid. By design, liposomes formed from the GDO lipid combine MRI tracking ability and dual-trigger release capabilities with maximum sensitivity (because all lipids bear the cleavable moiety) without reducing the drug encapsulation rate. We first confirmed that both photo-treatment and pH-triggered hydrolysis are able to cleave the GD...
Source: Biomaterials - August 6, 2019 Category: Materials Science Authors: Liu C, Ewert KK, Wang N, Li Y, Safinya CR, Qiao W Tags: Biomaterials Source Type: research

Fabrication of 3D scaffolds reproducing intestinal epithelium topography by high-resolution 3D stereolithography.
Abstract The small intestine is a complex tissue with a crypt/villus architecture and high tissue polarity. Maintenance of tissue integrity and function is supported by a constant renewal of the epithelium, with proliferative cells located in the crypts and differentiated cells migrating upward to the top of villi. So far, most in vitro studies have been limited to 2D surfaces or 3D organoid cultures that do not fully recapitulate the tissue 3D architecture, microenvironment and cell compartmentalization found in vivo. Here, we report the development of a 3D model that reproduces more faithfully the architecture o...
Source: Biomaterials - August 5, 2019 Category: Materials Science Authors: Creff J, Courson R, Mangeat T, Foncy J, Souleille S, Thibault C, Besson A, Malaquin L Tags: Biomaterials Source Type: research

In vivo and in vitro evaluation of a biodegradable magnesium vascular stent designed by shape optimization strategy.
Abstract The performance of biodegradable magnesium alloy stents (BMgS) requires special attention to non-uniform residual stress distribution and stress concentration, which can accelerate localized degradation after implantation. We now report on a novel concept in stent shape optimization using a finite element method (FEM) toolkit. A Mg-Nd-Zn-Zr alloy with uniform degradation behavior served as the basis of our BMgS. Comprehensive in vitro evaluations drove stent optimization, based on observed crimping and balloon inflation performance, measurement of radial strength, and stress condition validation via micro...
Source: Biomaterials - August 5, 2019 Category: Materials Science Authors: Chen C, Chen J, Wu W, Shi Y, Jin L, Petrini L, Shen L, Yuan G, Ding W, Ge J, Edelman ER, Migliavacca F Tags: Biomaterials Source Type: research

Improved zonal chondrocyte production protocol integrating size-based inertial spiral microchannel separation and dynamic microcarrier culture for clinical application.
This study demonstrates a novel scale-up zonal chondrocyte production protocol, incorporating size-based zonal chondrocyte separation and dMC platform, to maintain zonal chondrocytes' phenotypes better to support zonal repair of articular cartilage. PMID: 31404789 [PubMed - as supplied by publisher] (Source: Biomaterials)
Source: Biomaterials - August 5, 2019 Category: Materials Science Authors: Tee CA, Yang Z, Yin L, Wu Y, Han J, Lee EH Tags: Biomaterials Source Type: research

Oxygen-supplementing mesoporous polydopamine nanosponges with WS2 QDs-embedded for CT/MSOT/MR imaging and thermoradiotherapy of hypoxic cancer.
Abstract Multifunctional nanoplatforms with flexible architectures and tumor microenvironment response are highly anticipated within the field of thermoradiotherapy. Herein, the multifunctional nanoplatforms for thermoradiotherapy have been successfully constructed by the embedding of tungsten disulfide quantum dots (WS2 QDs) into mesoporous polydopamine nanosponges (MPDA NSs), followed by integration with manganese dioxide (MnO2). MPDA-WS2@MnO2, the resultant nanoplatforms, exhibit radiosensitization enhanced behavior and a capacity for responsive oxygen self-supplementation. The ingenious mesoporous structure of...
Source: Biomaterials - August 5, 2019 Category: Materials Science Authors: Wang Y, Song S, Lu T, Cheng Y, Song Y, Wang S, Tan F, Li J, Li N Tags: Biomaterials Source Type: research

IFN- γ-tethered hydrogels enhance mesenchymal stem cell-based immunomodulation and promote tissue repair.
In this study, we engineered an injectable synthetic hydrogel with tethered recombinant IFN-γ that activates encapsulated hMSCs to increase their immunomodulatory functions and avoids the need for ex vivo manipulation. Tethering IFN-γ to the hydrogel increases retention of IFN-γ within the biomaterial while preserving its biological activity. hMSCs encapsulated within hydrogels with tethered IFN-γ exhibited significant differences in cytokine secretion and showed a potent ability to halt activated T-cell proliferation and monocyte-derived dendritic cell differentiation compared to hMSCs that were pr...
Source: Biomaterials - August 2, 2019 Category: Materials Science Authors: García JR, Quirós M, Han WM, O'Leary MN, Cox GN, Nusrat A, García AJ Tags: Biomaterials Source Type: research

A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies.
AS Abstract Alterations of the microbial composition in the gut and the concomitant dysregulation of the mucosal immune response are associated with the pathogenesis of opportunistic infections, chronic inflammation, and inflammatory bowel disease. To create a platform for the investigation of the underlying mechanisms, we established a three-dimensional microphysiological model of the human intestine. This model resembles organotypic microanatomical structures and includes tissue resident innate immune cells exhibiting features of mucosal macrophages and dendritic cells. The model displays the physiological immu...
Source: Biomaterials - August 2, 2019 Category: Materials Science Authors: Maurer M, Gresnigt MS, Last A, Wollny T, Berlinghof F, Pospich R, Cseresnyes Z, Medyukhina A, Graf K, Gröger M, Raasch M, Siwczak F, Nietzsche S, Jacobsen ID, Figge MT, Hube B, Huber O, Mosig AS Tags: Biomaterials Source Type: research

Atializumab, a humanized anti-aminoacyl-tRNA synthetase-interacting multifunctional protein-1 (AIMP1) antibody significantly improves nephritis in (NZB/NZW) F1 mice.
Abstract Aminoacyl-tRNA synthetase (ARS)-interacting multifunctional protein 1 (AIMP1) enhances the expression of proinflammatory cytokines. In our previous study, we have shown that serum AIMP1 in patients with SLE was significantly higher than that of healthy controls. To address whether neutralization of AIMP1 could ameliorate nephritis in lupus-prone mice, we generated atializumab, a humanized antibody against AIMP1 and investigated its therapeutic efficacy. ELISA showed that serum AIMP1 at 23 weeks old was significantly higher than that at 13 weeks old in lupus-prone mice. Therefore, lupus-prone mice were ran...
Source: Biomaterials - August 2, 2019 Category: Materials Science Authors: Mun CH, Kim JO, Ahn SS, Yoon T, Kim SJ, Ko E, Noh HD, Park YB, Jung HJ, Kim TS, Lee SW, Park SG Tags: Biomaterials Source Type: research

Corrigendum to "Microwave pumped high-efficient thermoacoustic tumor therapy with single wall carbon nanotubes" [Biomaterials 75 (2016) 163-173].
Corrigendum to "Microwave pumped high-efficient thermoacoustic tumor therapy with single wall carbon nanotubes" [Biomaterials 75 (2016) 163-173]. Biomaterials. 2019 Aug 02;219:119392 Authors: Wen L, Ding W, Yang S, Xing D PMID: 31382207 [PubMed - as supplied by publisher] (Source: Biomaterials)
Source: Biomaterials - August 2, 2019 Category: Materials Science Authors: Wen L, Ding W, Yang S, Xing D Tags: Biomaterials Source Type: research

3D inkjet printing of biomaterials with strength reliability and cytocompatibility: Quantitative process strategy for Ti-6Al-4V.
Abstract Among additive manufacturing (AM) techniques, laser or electron beam based processes have been widely investigated for metallic implants. Despite the potential in manufacturing of patient-specific biomedical implants, 3D inkjet powder printing (3DIJPP, a variant of AM) of biomaterials is still in its infancy, as little is known quantitatively about the transient process physics and dynamics. An equally important challenge has been the ink formulation to manufacture biomaterials with reliable mechanical properties and desired biocompatibility. We have developed, for the very first time, the theoretical fou...
Source: Biomaterials - June 5, 2019 Category: Materials Science Authors: Barui S, Panda AK, Naskar S, Kuppuraj R, Basu S, Basu B Tags: Biomaterials Source Type: research

Corrigendum to: "Gold and gold-silver alloy nanoparticles enhance the myogenic differentiation of myoblasts through p38 MAPK signaling pathway and promote in vivo skeletal muscle regeneration" [Biomaterials 175(2018)19-29].
Corrigendum to: "Gold and gold-silver alloy nanoparticles enhance the myogenic differentiation of myoblasts through p38 MAPK signaling pathway and promote in vivo skeletal muscle regeneration" [Biomaterials 175(2018)19-29]. Biomaterials. 2019 Jun;204:80-81 Authors: Ge J, Liu K, Niu W, Chen M, Wang M, Xue Y, Gao C, Ma PX, Lei B PMID: 30928127 [PubMed - in process] (Source: Biomaterials)
Source: Biomaterials - April 2, 2019 Category: Materials Science Authors: Ge J, Liu K, Niu W, Chen M, Wang M, Xue Y, Gao C, Ma PX, Lei B Tags: Biomaterials Source Type: research