The porin AaxA protein model from Chlamydia pneumonia
Bioinformation. 2020 Oct 31;16(10):786-788. doi: 10.6026/97320630016786. eCollection 2020.ABSTRACTChlamydophila pneumoniae is an intracellular pathogen accountable for various acute respiratory infections. C. pneumoniae has a gene cluster which encodes a putative outer membrane porin (aaxA), arginine decarboxylase (CPn1032 or aaxB) and a putative cytoplasmic membrane transporter (CPn1031 or aaxC). Therefore, it is of interest to document a molecular protein model of porin AaxA from Chlamydia pneumonia to gain structure to functional insight on the protein.PMID:34675464 | PMC:PMC8503770 | DOI:10.6026/97320630016786 (Source: Bioinformation)
Source: Bioinformation - October 22, 2021 Category: Bioinformatics Authors: Jayaraman Selvaraj Srinivasan Perumal Josephine Rex Surapaneni Krishna Mohan Sumetha Suga Deiva Suga Umapathy Vidhya Rekh Veeraraghavan Vishnupriya Periyasamy Vijayalakshmi Rajagopal Ponnulakshmi Source Type: research
The Analysis of Herpes Simplex Virus Type 1 (HSV-1)-Encoded MicroRNAs Targets: A Likely Relationship of Alzheimer's Disease and HSV-1 Infection
Cell Mol Neurobiol. 2021 Oct 18. doi: 10.1007/s10571-021-01154-8. Online ahead of print.ABSTRACTAlzheimer's disease (AD), the most frequently diagnosed dementia, is a senile neurodegenerative disorder characterized by amnesia and cognitive dysfunction. Unfortunately, there are still no successful strategies to prevent AD progression. Thus, the vast majority of research focuses on recognizing risk factors for developing and progressing this disease. Human spirochetes, fungi, Borrelia burgdorferi, Chlamydophila pneumoniae, Helicobacter pylori, and human herpes simplex virus type 1 (HSV-1) have all been implicated in the deve...
Source: Cellular and Molecular Neurobiology - October 18, 2021 Category: Cytology Authors: Nima Hemmat Haniyeh Asadzadeh Zahra Asadzadeh Mahdi Abdoli Shadbad Behzad Baradaran Source Type: research
Association of Chlamydophila pneumoniae infection and hypertension during pregnancy - A case control study
CONCLUSION: There is a significant association between C. pneumoniae infection and hypertension during pregnancy and further studies are required to fulfil the Koch's postulates to prove or disprove it as a causative agent.PMID:34433341 | DOI:10.1080/10641963.2021.1969661 (Source: Clinical and Experimental Hypertension)
Source: Clinical and Experimental Hypertension - August 26, 2021 Category: Cardiology Authors: Bhagyashree Gundagurti Papa Dasari Rakesh Singh Source Type: research
Association of Chlamydophila pneumoniae infection and hypertension during pregnancy - A case control study
CONCLUSION: There is a significant association between C. pneumoniae infection and hypertension during pregnancy and further studies are required to fulfil the Koch's postulates to prove or disprove it as a causative agent.PMID:34433341 | DOI:10.1080/10641963.2021.1969661 (Source: Clinical and Experimental Hypertension)
Source: Clinical and Experimental Hypertension - August 26, 2021 Category: Cardiology Authors: Bhagyashree Gundagurti Papa Dasari Rakesh Singh Source Type: research
A portable centrifugal genetic analyzer for multiplex detection of feline upper respiratory tract disease pathogens
We present a portable genetic analyzer with an integrated centrifugal disc which is equipped with a glass-filter extraction column for purifying nucleic acid (NA) and multiple reaction chambers for analyzing major feline upper respiratory tract disease (FURTD) pathogens. We targeted four kinds of FURTD including Feline herpesvirus 1 (FHV), Mycoplasma felis (MPF), Bordetella bronchiseptica (BDB), and Chlamydophila felis (CDF). The portable genetic analyzer consists of a spinning motor, two pairs of Peltier heaters, two Minco heater, fluorescent optics, a touchscreen, and software for data analysis, so loop-mediated isotherm...
Source: Biosensors and Bioelectronics - August 14, 2021 Category: Biotechnology Authors: Hau Van Nguyen Vu Minh Phan Tae Seok Seo Source Type: research
A portable centrifugal genetic analyzer for multiplex detection of feline upper respiratory tract disease pathogens
We present a portable genetic analyzer with an integrated centrifugal disc which is equipped with a glass-filter extraction column for purifying nucleic acid (NA) and multiple reaction chambers for analyzing major feline upper respiratory tract disease (FURTD) pathogens. We targeted four kinds of FURTD including Feline herpesvirus 1 (FHV), Mycoplasma felis (MPF), Bordetella bronchiseptica (BDB), and Chlamydophila felis (CDF). The portable genetic analyzer consists of a spinning motor, two pairs of Peltier heaters, two Minco heater, fluorescent optics, a touchscreen, and software for data analysis, so loop-mediated isotherm...
Source: Biosensors and Bioelectronics - August 14, 2021 Category: Biotechnology Authors: Hau Van Nguyen Vu Minh Phan Tae Seok Seo Source Type: research
A portable centrifugal genetic analyzer for multiplex detection of feline upper respiratory tract disease pathogens
We present a portable genetic analyzer with an integrated centrifugal disc which is equipped with a glass-filter extraction column for purifying nucleic acid (NA) and multiple reaction chambers for analyzing major feline upper respiratory tract disease (FURTD) pathogens. We targeted four kinds of FURTD including Feline herpesvirus 1 (FHV), Mycoplasma felis (MPF), Bordetella bronchiseptica (BDB), and Chlamydophila felis (CDF). The portable genetic analyzer consists of a spinning motor, two pairs of Peltier heaters, two Minco heater, fluorescent optics, a touchscreen, and software for data analysis, so loop-mediated isotherm...
Source: Biosensors and Bioelectronics - August 14, 2021 Category: Biotechnology Authors: Hau Van Nguyen Vu Minh Phan Tae Seok Seo Source Type: research
Design and Evaluation of Multiplex One-Step Reverse Transcription PCR-Dipstick Chromatography Method for the Analysis of Seven Respiratory Pathogens
The objective of this study was to design and evaluate a sensitive and specific multiplex one-step reverse transcription PCR (RT-PCR)-dipstick chromatography method for simultaneous rapid detection of these seven pathogens. Streptavidin-coated blue latex particles were used to read out a positive signal. Based on the DNA-DNA hybridization of oligonucleotide sequences (Tag) for forward primer with the complementary oligonucleotide sequence (cTag) on the dipstick and biotin-streptavidin interactions, PCR products were able to be illuminated visually on the dipstick. The specificity and the limit of detection (LOD) were also ...
Source: Current Microbiology - August 2, 2021 Category: Microbiology Authors: Li Luo Qianming Chen Sheng Qin Qiang Luo Zhenjie Liu Qiong Li Shuilan Zheng Xianzhang Huang Peifeng Ke Xiangsheng Yang Hui Xiao Ning Xu Source Type: research
Design and Evaluation of Multiplex One-Step Reverse Transcription PCR-Dipstick Chromatography Method for the Analysis of Seven Respiratory Pathogens
The objective of this study was to design and evaluate a sensitive and specific multiplex one-step reverse transcription PCR (RT-PCR)-dipstick chromatography method for simultaneous rapid detection of these seven pathogens. Streptavidin-coated blue latex particles were used to read out a positive signal. Based on the DNA-DNA hybridization of oligonucleotide sequences (Tag) for forward primer with the complementary oligonucleotide sequence (cTag) on the dipstick and biotin-streptavidin interactions, PCR products were able to be illuminated visually on the dipstick. The specificity and the limit of detection (LOD) were also ...
Source: Current Microbiology - August 2, 2021 Category: Microbiology Authors: Li Luo Qianming Chen Sheng Qin Qiang Luo Zhenjie Liu Qiong Li Shuilan Zheng Xianzhang Huang Peifeng Ke Xiangsheng Yang Hui Xiao Ning Xu Source Type: research
Predicting Mycoplasma pneumoniae and Chlamydophila pneumoniae in community-acquired pneumonia (CAP) pneumonia: epidemiological study of respiratory tract infection using multiplex PCR assays
AbstractCommunity-acquired pneumonia (CAP) is a common illness that can lead to mortality. β-lactams are ineffective against atypical pathogen includingMycoplasma pneumoniae. We used molecular examinations to develop a decision tree to predict atypical pathogens with CAP and to examine the prevalence of macrolide resistance inMycoplasma pneumoniae. We conducted a prospective observational study of patients aged ≥ 18 years who had fever and respiratory symptoms and were diagnosed with CAP in one of two community hospitals between December 2016 and October 2018. We assessed combinations of clinical variables that be...
Source: Internal and Emergency Medicine - May 13, 2021 Category: Emergency Medicine Source Type: research
Sputum microbiota as a potential diagnostic marker for multidrug-resistant tuberculosis
This study aims to use 16 rRNA sequencing technology to detect and analyze upper respiratory flora of TB patients with anti-TB drug sensitivity (DS, n = 55), monoresistance isoniazide (MR-INH, n = 33), monoresistance rifampin (MR-RFP, n = 12), multidrug resistance (MDR, n = 26) and polyresistance (PR, n = 39) in southern China. Potential microbial diagnostic markers for different types of TB drug resistance are searched by screening differential flora, which provides certain guiding significance for drug resistance diagnosis and clinical drug use of TB. The results showed that the pulmonary microenvironment of TB patients ...
Source: International Journal of Medical Sciences - April 14, 2021 Category: Biomedical Science Authors: Dongzi Lin Xuezhi Wang Yanyun Li Wei Wang Yumei Li Xiaolin Yu Bingyao Lin Yinwen Chen Chunyan Lei Xueying Zhang Xilin Zhang Juan Huang Bihua Lin Weiqing Yang Jie Zhou Jincheng Zeng Xinguang Liu Source Type: research
Viruses, Vol. 13, Pages 618: A pre- and during Pandemic Survey of Sars-Cov-2 Infection in Stray Colony and Shelter Cats from a High Endemic Area of Northern Italy
niela Proverbio
Cats are susceptible to infection with severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2). Whilst a number of studies have been performed worldwide on owned cats, limited data are available on stray, colony or shelter cats. We investigated SARS-CoV-2 infection in a stray cat population before and during human outbreaks of SARS-CoV-2 in cities in the Lombardy region in northern Italy, a high endemic region for SARS-CoV-2, using serological and molecular methods. A cohort of different samples were collected from 241 cats, including frozen archived serum samples from 136 cats collected before the...
Source: Viruses - April 3, 2021 Category: Virology Authors: Eva Spada Fabrizio Vitale Federica Bruno Germano Castelli Stefano Reale Roberta Perego Luciana Baggiani Daniela Proverbio Tags: Article Source Type: research
Mucosal Respiratory Syndrome: A Systematic Literature Review
Background:Mycoplasma pneumoniae atypical pneumonia is frequently associated with erythema multiforme. Occasionally, a mycoplasma infection does not trigger any cutaneous but exclusively mucosal lesions. The term mucosal respiratory syndrome is employed to denote the latter condition. Available reviews do not address the possible association of mucosal respiratory syndrome with further atypical bacterial pathogens such asChlamydophila pneumoniae,Chlamydophila psittaci,Coxiella burnetii,Francisella tularensis, orLegionella species. We therefore performed a systematic review of the literature addressing this issue in the Nat...
Source: Dermatology - March 26, 2021 Category: Dermatology Source Type: research
The role of typical and atypical pathogens in acute exacerbations of chronic obstructive pulmonary disease
ConclusionThe additional use of DFA forLegionella and multiplex PCR in combination with nonspecific microbiological culturing methods greatly improves the ability to identify infectious agents in acute exacerbations of COPD. There should be a high index of suspicion forP.aeruginosa as a causative organism, particularly in subjects receiving continuous oxygen therapy and/or using NIV andL. pneumophila should certainly be taken into consideration in severe COPD exacerbations. (Source: The Clinical Respiratory Journal)
Source: The Clinical Respiratory Journal - February 20, 2021 Category: Respiratory Medicine Authors: Sevilay Yildiz,
Nevriye Gonullu,
Birsen Pinar Yildiz,
Didem Gorgun Hattatoglu,
Mert Kuskucu,
Kenan Midilli,
Gokhan Aygun,
Pelin Yuksel Mayda,
Benan Musellim Tags: ORIGINAL ARTICLE Source Type: research
