Cellular and Tissue Selectivity of AAV Serotypes for Gene Delivery to Chondrocytes and Cartilage
Conclusions: Our findings indicate that AAV2 and AAV6.2 may be the best choices for AAV-mediated gene delivery into intra-articular cartilage tissue. These AAV vectors hold the potential to be of use in clinical applications to prevent OA progression if appropriate therapeutic genes are inserted into the vector.PMID:34522160 | PMC:PMC8436087 | DOI:10.7150/ijms.56760 (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Dong Suk Yoon Kyoung-Mi Lee Sehee Cho Eun Ae Ko Jihyun Kim Sujin Jung Jae-Hyuck Shim Guangping Gao Kwang Hwan Park Jin Woo Lee Source Type: research

Ferroptosis and Liver Fibrosis
Int J Med Sci. 2021 Jul 25;18(15):3361-3366. doi: 10.7150/ijms.62903. eCollection 2021.ABSTRACTFerroptosis is an iron-dependent form of regulated cell death, which is characterized by a large amount of lipid peroxide accumulation and the imbalance of redox state in cells. Ferroptosis is usually accompanied with the dysfunction of lipid repair enzyme (glutathione peroxidase 4, GPX4), large masses of iron accumulation and lipid peroxidation of polyunsaturated fatty acids (PUFAs). Ferroptosis is related to several signaling pathways, including amino acid and iron metabolism, ferritinophagy, cell adhesion and p53 and Keap1/Nrf...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Qi Pan Yi Luo Qiang Xia Kang He Source Type: research

Antenatal Betamethasone Induces Increased Surfactant Proteins and Decreased Foxm1 Expressions in Fetal Rabbit Pups
Conclusion: Decreased Foxm1 expression was associated in an antenatal betamethasone animal model.PMID:34522162 | PMC:PMC8436089 | DOI:10.7150/ijms.62286 (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Yong-Sung Choi Chae-Young Kim Hye Kyung Chang Young Joo Lee Sung-Hoon Chung Source Type: research

Otorhinolaryngological Management in Taiwanese Patients with Mucopolysaccharidoses
Conclusions: MPS patients had a high incidence of ENT problems. ENT surgery reduced the severity of hearing loss, degree of symptoms related to upper airway obstruction, and severity of respiratory tract and otological infections of patients with MPS.PMID:34522163 | PMC:PMC8436092 | DOI:10.7150/ijms.61827 (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Chung-Lin Lee Kuo-Sheng Lee Chih-Kuang Chuang Chin-Hui Su Huei-Ching Chiu Ru-Yi Tu Yun-Ting Lo Ya-Hui Chang Hsiang-Yu Lin Shuan-Pei Lin Source Type: research

The combination therapy with EpCAM/CD3 BsAb and MUC-1/CD3 BsAb elicited antitumor immunity by T-cell adoptive immunotherapy in lung cancer
In this study, we combined two BsAbs to achieve a dual-target therapy strategy of EpCAM+ and MUC-1+ with high affinity and specificity. The results showed that the combination of two BsAbs against EpCAM and MUC-1 could inhibit the growth of lung cancer more effectively in cell lines and primary tumors. The superior antitumor effect of two BsAbs could be attributable to enhanced CTL and increased production of type I IFNs. At the same time, the combination of EpCAM/CD3 BsAb and MUC-1/CD3 BsAb significantly regulated T population in the TDLNs. Therefore, we have found a potential immunotherapeutic strategy, which was the com...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Ce Wang Shang Chen Yingjuan Wu Di Wu Jingbo Wang Furong Li Source Type: research

Hypothesis: Immunotherapy by Selective Convalescent Blood Engineering to Stifle Diseases like COVID-19
This report incorporates all these aspects by suggesting a novel treatment therapy called selective convalescent leukapheresis and transfusion (SCLT) and also highlights its potential in vaccination. The anticipated advantages of the proposed technique outweigh the cost, time, and efficiency of other available transfusion and vaccination processes. It is envisioned that in the future this new approach could serve as a rapid emergency response to subdue a pathogen outbreak and to stop it from becoming an epidemic, or pandemic.PMID:34522165 | PMC:PMC8436102 | DOI:10.7150/ijms.46363 (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Arka Prava Mukherjee Source Type: research

Chest CT abnormalities in COVID-19: a systematic review
Int J Med Sci. 2021 Aug 1;18(15):3395-3402. doi: 10.7150/ijms.50568. eCollection 2021.ABSTRACTComputed tomography (CT) of the chest is one of the main diagnositic tools for coronavirus disease 2019 (COVID-19) infection. To document the chest CT findings in patients with confirmed COVID-19 and their association with the clinical severity, we searched related literatures through PubMed, MEDLINE, Embase, Web of Science (inception to May 4, 2020) and reviewed reference lists of previous systematic reviews. A total of 31 case reports (3768 patients) on CT findings of COVID-19 were included. The most common comorbid conditions w...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Ramy Abou Ghayda Keum Hwa Lee Jae Seok Kim Seul Lee Sung Hwi Hong Kyeong Seok Kim Kyeong Eon Kim Jinhyn Seok Hajeong Kim Jangsuk Seo Seungmin Lee Ai Koyanagi Louis Jacob Lee Smith Han Li Andreas Kronbichler Jae Il Shin Source Type: research

Cobalt chloride-stimulated hypoxia promotes the proliferation of cholesteatoma keratinocytes via the PI3K/Akt signaling pathway
Int J Med Sci. 2021 Aug 2;18(15):3403-3411. doi: 10.7150/ijms.60617. eCollection 2021.ABSTRACTHerein, we purposed to explore whether hypoxia triggers proliferation of cholesteatoma keratinocytes via the PI3K-Akt signaling cascade. Cells were inoculated with different concentration of CoCl2. The proliferation and cellular HIF-1α, p-PDK1 and p‑Akt expression levels of cholesteatoma keratinocytes were assessed in vitro. Hypoxia escalated cell proliferation via upregulating p-PDK1 and p‑Akt expressions. Specific inhibitor of the PI3K-Akt signaling cascade, LY294002 markedly inhibited the expression of p‑Akt and sig...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Chen Zhang Min Chen Qi Tao Zhangcai Chi Source Type: research

Integrated analysis of the molecular mechanisms in idiopathic pulmonary fibrosis
Conclusion: These findings provide a new research direction for the diagnosis and treatment of IPF.PMID:34522168 | PMC:PMC8436110 | DOI:10.7150/ijms.61309 (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Ke Zhu Aiqun Xu Wanli Xia Pulin Li Rui Han Enze Wang Sijing Zhou Ran Wang Source Type: research

Identification of Key Genes and Pathways associated with Endometriosis by Weighted Gene Co-expression Network Analysis
Conclusion: Our study identified, for the first time, the hub genes and enrichment pathways in the EC and EU using WGCNA, which may provide a comprehensive understanding of the pathogenesis of endometriosis and have important clinical implications for the treatment and diagnosis of endometriosis.PMID:34522169 | PMC:PMC8436105 | DOI:10.7150/ijms.63541 (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Jingni Wu Xiaoling Fang Xiaomeng Xia Source Type: research

Simultaneous silencing Aurora-A and UHRF1 inhibits colorectal cancer cell growth through regulating expression of DNMT1 and STAT1
Int J Med Sci. 2021 Aug 5;18(15):3437-3451. doi: 10.7150/ijms.61969. eCollection 2021.ABSTRACTAurora-A has attracted a great deal of interest as a potential therapeutic target for patients with CRC. However, the outcomes of inhibitors targeting Aurora-A are not as favorable as expected, and the basis behind the ineffectiveness remains unknown. Here, we found that signal transducer and activator of transcription 1 (STAT1) was highly expressed in colorectal cancer (CRC) xenograft mouse models that were resistant to alisertib, an Aurora-A inhibitor. Unexpectedly, we found that alisertib disrupted Aurora-A binding with ubiquit...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Jing Han Xin Chen Jiawei Xu Laili Chu Rongqing Li Na Sun Zhen Jiang Hongyang Liu Xing Ge Junnian Zheng Jing Yang Takayuki Ikezoe Source Type: research

Areca nut extract (ANE) inhibits the progression of hepatocellular carcinoma cells via activation of ROS production and activation of autophagy
In conclusion, our study demonstrated that the ANE may be a new potential compound for HCC therapy.PMID:34522171 | PMC:PMC8436086 | DOI:10.7150/ijms.61570 (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Po-Li Wei Chin-Sheng Hung Hsuan-Hsuan Lu Uyanga Batzorig Chien-Yu Huang Yu-Jia Chang Source Type: research

Verification of the Efficacy of New Diagnostic Criteria for Retropharyngeal Nodes in a Cohort of Nasopharyngeal Carcinoma Patients
Int J Med Sci. 2021 Aug 13;18(15):3463-3469. doi: 10.7150/ijms.58375. eCollection 2021.ABSTRACTPurpose: A multistage approach to diagnose lateral retropharyngeal nodes (LRPNs) of nasopharyngeal carcinoma (NPC) had been proposed and warranted for validation. Methods: Between 2012 and 2017, the patients with newly diagnosed NPC were enrolled. The responsive nodes or those that progressed during follow-up were positive. The criteria for the multistage approach delimited LRPNs with a minimal axial diameter (MIAD) ≥ 6.1 mm were assessed as positive and if the mean standard uptake value ≥ 2.6, or if the maximal coronal dia...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Dom-Gene Tu Hsuan-Yu Chen Wei-Jen Yao Yu-Sheng Hung Yu-Kang Chang Chih-Han Chang Yu-Wen Wang Source Type: research

Sex Difference in the Associations among Obesity-Related Indices with Metabolic Syndrome in Patients with Type 2 Diabetes Mellitus
Int J Med Sci. 2021 Aug 13;18(15):3470-3477. doi: 10.7150/ijms.63180. eCollection 2021.ABSTRACTBackground: The aim of this study was to investigate the associations among obesity-related indices and MetS in diabetic patients, and explore sex differences in these associations. Methods: Patients with type 2 DM were included from two hospitals in southern Taiwan. The Adult Treatment Panel III criteria for an Asian population were used to define MetS. In addition, the following obesity-related indices were evaluated: waist-to-height ratio, waist-hip ratio (WHR), conicity index (CI), body mass index (BMI), body roundness index,...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Hsiu-Fen Jao Chih-Hsuan Wung Hui-Chen Yu Mei-Yueh Lee Po-Chih Chen Szu-Chia Chen Jer-Ming Chang Source Type: research

Bioinformatics analysis identified shared differentially expressed genes as potential biomarkers for Hashimoto's thyroiditis-related papillary thyroid cancer
Conclusions: We identified that expression of LTF and CCL21 are significantly different in HT and PTC, suggesting an underlying association between HT and PTC.PMID:34522174 | PMC:PMC8436097 | DOI:10.7150/ijms.63402 (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Chang Liu Yu Pan Qinyu Li Yifan Zhang Source Type: research

Comparison of short- and long-axis nerve hydrodissection for carpal tunnel syndrome: A prospective randomized, single-blind trial
This study is to compare the efficacy of short-axis hydrodissection with long-axis hydrodissection for patients with mild-to-moderate carpal tunnel syndrome (CTS). Methods: Forty-seven patients with mild-to-moderate CTS were enrolled in a prospective, randomized, single-blinded, controlled trial (6 months follow-up). With ultrasound guidance, patients in both groups (short-axis or long-axis groups) were injected with normal saline (5 mL per session). Assessments were performed before and 2 weeks after the injection, as well as at 1, 3, and 6 months post-intervention. The primary outcome measure was the Boston Carpal Tunnel...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Si-Ru Chen Tsung-Yen Ho Yu-Ping Shen Tsung-Ying Li Yu-Chi Su King Hei Stanley Lam Liang-Cheng Chen Yung-Tsan Wu Source Type: research

Expression and localization of Sox10 during hair follicle morphogenesis and induced hair cycle
Int J Med Sci. 2021 Aug 13;18(15):3498-3505. doi: 10.7150/ijms.60728. eCollection 2021.ABSTRACTSox transcription factors play many diverse roles during development, including regulating stem cell states, directing differentiation, and influencing the local chromatin landscape. Sox10 has been implicated in the control of stem/progenitor activity and epithelial-mesenchymal transition, yet it has not been studied in relation to the hair follicle cycle or hair follicle stem cell (HFSC) control. To elucidate the role of Sox10 in hair follicle cycle control, we performed immunohistochemical and immunofluorescence analysis of its...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Jing Jing Peng Xu Jia-Li Xu Yu-Xin Ding Xiao-Shuang Yang Xiao-Qin Jin Li-Juan Zhou Yu-Hong Chen Xian-Jie Wu Zhong-Fa Lu Source Type: research

Downregulation of circ_0000673 Promotes Cell Proliferation and Migration in Endometriosis via the Mir-616-3p/PTEN Axis
Int J Med Sci. 2021 Aug 17;18(15):3506-3515. doi: 10.7150/ijms.63564. eCollection 2021.ABSTRACTEndometriosis is a common gynecological disease, affecting up to 10% of women of reproductive age and approximately 50% of women with infertility. Circular RNAs (circRNAs) have been shown to be involved in a number of diseases. Dysregulated expression of circRNAs in endometriosis has been reported, and circ_0000673 was significantly downregulated. However, the details of its role in the pathogenesis of endometriosis are still poorly understood. We investigated the location and effects of the downregulation of circ_0000673 in endo...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Yongwen Yang Deying Ban Chun Zhang Licong Shen Source Type: research

PLK1 Inhibition Induces Immunogenic Cell Death and Enhances Immunity against NSCLC
In this study, we found that inhibiting PLK1 could alter the tumor immune microenvironment by increasing DC maturation, and enriching T cells infiltration. PLK1 inhibitors, serving as immunogenic cell death (ICD) inducers, indirectly activated DCs, instead of directly acting on DC cells, through the surface expression of costimulatory molecules on and enhanced phagocytosis by DCs. Furthermore, upon targeting PLK1, tumor cells that had undergone ICD were converted into an endogenous vaccine, which triggered the immune memory responses and protected the mice from tumor challenge. Collectively, these results suggested that th...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Jie Zhou Qifan Yang Lisen Lu Zhan Tuo Zhexing Shou Jing Cheng Source Type: research

Risk factor comparison in young patients presenting with acute coronary syndrome with atherosclerotic coronary artery disease vs. angiographically normal coronaries
Int J Med Sci. 2021 Aug 21;18(15):3526-3532. doi: 10.7150/ijms.60869. eCollection 2021.ABSTRACTBackground: Acute myocardial infarction is a relatively rare phenomenon in the young population. The incidence has nevertheless increased from years past, likely due to the presence of multiple risk factors from an increasingly younger age. Regardless of whether they have atherosclerotic coronary artery disease or normal coronary angiogram, young patients with risk factors for coronary artery disease (CAD), chest pain, and positive troponin, are initially treated in a similar fashion. Our goal was to shed light on whether risk fa...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Sarah Jamil Gohar Jamil Hanaa Mesameh Anwer Qureshi Juma AlKaabi Charu Sharma Faisal Aziz Ali Rashed Al-Shamsi Javed Yasin Source Type: research

Possible mechanisms of cholesterol elevation aggravating COVID-19
Conclusions and Relevance: Cholesterol can indirectly increase the susceptibility of patients to SARS-CoV-2 and increase the risk of death from COVID-19, which are mediated by NLRP3 and atherosclerotic plaques, respectively. Cholesterol present in the host cell membrane, virus, and blood may also directly participate in the virus cell entry process, but the specific mechanism still needs further study. Patients with COVID-19 are recommended to continue lipid-lowering therapy.PMID:34522180 | PMC:PMC8436106 | DOI:10.7150/ijms.62021 (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Yan Tang Longtai Hu Yi Liu Bangyi Zhou Xiaohuan Qin Jujian Ye Maoze Shen Zhijian Wu Peidong Zhang Source Type: research

Thymopentin treatment of murine premature ovarian failure via attenuation of immune cell activity and promotion of the BMP4/Smad9 signalling pathway
Int J Med Sci. 2021 Aug 21;18(15):3544-3555. doi: 10.7150/ijms.61975. eCollection 2021.ABSTRACTPremature ovarian failure (POF) is a typical form of pathological aging with complex pathogenesis and no effective treatment. Meanwhile, recent studies have reported that a high-fat and high-sugar (HFHS) diet adversely affects ovarian function and ovum quality. Here, we investigated the therapeutic effect of thymopentin (TP-5) as a treatment for murine POF derived from HFHS and its target. Pathological examination and hormone assays confirmed that TP-5 significantly improved murine POF symptoms. And, TP-5 could reduce oxidative s...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Xueqin Zhu Jianjun Liu Hao Pan Zixiang Geng William Huang Te Liu Bimeng Zhang Source Type: research

Roles of the miR-139-5p/CCT5 axis in hepatocellular carcinoma: a bioinformatic analysis
Int J Med Sci. 2021 Aug 25;18(15):3556-3564. doi: 10.7150/ijms.57504. eCollection 2021.ABSTRACTBackground: MiRNAs are pivotal regulators involved in proliferation, apoptosis, invasion, metastasis, epithelial-mesenchymal transition (EMT), angiogenesis, drug resistance and autophagy in hepatocellular carcinoma (HCC). The aim of this study was to investigate the influence of miR-139-5p and its target genes on the outcomes of HCC. Methods: Survival analysis of miR-139-5p in HCC was conducted in Kaplan-Meier plotter. Target genes of miR-139-5p were identified in TargetScan, miRTarBase and starBase. Gene Expression Omnibus (GEO)...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Jingjing Xu Yuan Zhang Cheng Liu Ping Yan Zongguo Yang Source Type: research

Risk of Pneumonia is associated with Antipsychotic Drug Use among older patients with Parkinson's Disease: A Case-control Study
Conclusion: Older patients with PD receiving typical antipsychotics or atypical antipsychotics had a higher risk of pneumonia. Among these antipsychotics, clozapine had the highest risk of pneumonia. Clinicians should pay attention to the risk of pneumonia in older patients with PD who receive typical antipsychotics and atypical antipsychotics.PMID:34522183 | PMC:PMC8436093 | DOI:10.7150/ijms.63246 (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Kuang-Hua Huang Wei-Yin Kuo Yu-Hsiang Kuan Yu-Chia Chang Tung-Han Tsai Chien-Ying Lee Source Type: research

Polypharmacy Is Associated With Amiodarone-Induced Hypothyroidism
In this study, we investigated the association between polypharmacy and AIH. Methods: We conducted a retrospective study using data from January 2006 to May 2020 collected from a large, organized database of prescriptions constructed by the Japan Medical Information Research Institute, Inc. (Tokyo, Japan). To investigate the association between number of prescribed drugs with amiodarone and AIH, we divided patients into two groups: polypharmacy (≥ 5 prescribed drugs) and non-polypharmacy (
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Satoshi Yokoyama Yuki Tanaka Kouichi Hosomi Mitsutaka Takada Source Type: research

Plasma levels of amino acids and derivatives in retinopathy of prematurity
Conclusion: The plasma levels of citrulline, creatinine, arginine, and aminoadipic acid were significantly changed in ROP patients. These metabolites could be considered potential biomarkers of ROP, and their related metabolic pathways might be involved in ROP pathogenesis.PMID:34522185 | PMC:PMC8436098 | DOI:10.7150/ijms.63603 (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Yedi Zhou Yu Xu Xiang Zhang Qian Huang Wei Tan Yonghui Yang Xiaori He Shigeo Yoshida Peiquan Zhao Yun Li Source Type: research

PRMT4 promotes hepatocellular carcinoma progression by activating AKT/mTOR signaling and indicates poor prognosis
Conclusions: This study suggests that PRMT4 may promote the progression of HCC cells by activating the AKT/mTOR signaling pathway, which may be a valuable biomarker and potential target for HCC.PMID:34522186 | PMC:PMC8436100 | DOI:10.7150/ijms.62467 (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Peng Du Kaifeng Luo Guoyong Li Jisheng Zhu Qi Xiao Yong Li Xingjian Zhang Source Type: research

Predictive performance of eLIFT for liver inflammation and fibrosis in chronic liver diseases
Conclusion: The eLIFT is a potentially useful noninvasive predictor of liver inflammation and fibrosis in patients with CLD.PMID:34522187 | PMC:PMC8436088 | DOI:10.7150/ijms.62386 (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Zongguo Yang Xin Ma Xinlan Zhou Dan Huang Yanbing Wang Xiufen Li Wei Lu Zhanqing Zhang Rongrong Ding Source Type: research

The Impact of Motor Disability and the Level of Fatigue on Adherence to Therapeutic Recommendations in Patients with Multiple Sclerosis Treated with Immunomodulation
Conclusion: It was found that the level of adherence to therapeutic recommendations in patients with relapsing-remitting multiple sclerosis treated with immunomodulation in the study group remained high. There is a relationship between the patient's disability and the level of adherence to therapeutic recommendations.PMID:34522188 | PMC:PMC8436112 | DOI:10.7150/ijms.61964 (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Robert Ślusarz Joanna Olkiewicz Robert Bonek Karolina Filipska Monika Biercewicz Adam Wi śniewski Source Type: research

Role of "Stiff Rim" sign obtained by shear wave elastography in diagnosis and guiding therapy of breast cancer
This study aimed to investigate the usefulness of the stiff rim sign in the diagnosis and tumor, node, metastasis stage of breast cancer. Methods: Two hundred and ten breast lesions were analyzed retrospectively. The maximum, mean, minimum Young's modulus (YM), and the YM standard deviation in the lesion, the peritumoral stiffness (shell), and the region containing lesion and shell were obtained. The suspicious SWE feature with the best diagnostic performance was chosen to downgrade or upgrade the Breast Imaging Reporting and Data System (BI-RADS) classification. The coincidence rates of SWE and B-mode ultrasound in T stag...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Yan-Jun Xu Hui-Ling Gong Bin Hu Bing Hu Source Type: research

Cellular and Tissue Selectivity of AAV Serotypes for Gene Delivery to Chondrocytes and Cartilage
Conclusions: Our findings indicate that AAV2 and AAV6.2 may be the best choices for AAV-mediated gene delivery into intra-articular cartilage tissue. These AAV vectors hold the potential to be of use in clinical applications to prevent OA progression if appropriate therapeutic genes are inserted into the vector.PMID:34522160 | PMC:PMC8436087 | DOI:10.7150/ijms.56760 (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Dong Suk Yoon Kyoung-Mi Lee Sehee Cho Eun Ae Ko Jihyun Kim Sujin Jung Jae-Hyuck Shim Guangping Gao Kwang Hwan Park Jin Woo Lee Source Type: research

Ferroptosis and Liver Fibrosis
Int J Med Sci. 2021 Jul 25;18(15):3361-3366. doi: 10.7150/ijms.62903. eCollection 2021.ABSTRACTFerroptosis is an iron-dependent form of regulated cell death, which is characterized by a large amount of lipid peroxide accumulation and the imbalance of redox state in cells. Ferroptosis is usually accompanied with the dysfunction of lipid repair enzyme (glutathione peroxidase 4, GPX4), large masses of iron accumulation and lipid peroxidation of polyunsaturated fatty acids (PUFAs). Ferroptosis is related to several signaling pathways, including amino acid and iron metabolism, ferritinophagy, cell adhesion and p53 and Keap1/Nrf...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Qi Pan Yi Luo Qiang Xia Kang He Source Type: research

Antenatal Betamethasone Induces Increased Surfactant Proteins and Decreased Foxm1 Expressions in Fetal Rabbit Pups
Conclusion: Decreased Foxm1 expression was associated in an antenatal betamethasone animal model.PMID:34522162 | PMC:PMC8436089 | DOI:10.7150/ijms.62286 (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Yong-Sung Choi Chae-Young Kim Hye Kyung Chang Young Joo Lee Sung-Hoon Chung Source Type: research

Otorhinolaryngological Management in Taiwanese Patients with Mucopolysaccharidoses
Conclusions: MPS patients had a high incidence of ENT problems. ENT surgery reduced the severity of hearing loss, degree of symptoms related to upper airway obstruction, and severity of respiratory tract and otological infections of patients with MPS.PMID:34522163 | PMC:PMC8436092 | DOI:10.7150/ijms.61827 (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Chung-Lin Lee Kuo-Sheng Lee Chih-Kuang Chuang Chin-Hui Su Huei-Ching Chiu Ru-Yi Tu Yun-Ting Lo Ya-Hui Chang Hsiang-Yu Lin Shuan-Pei Lin Source Type: research

The combination therapy with EpCAM/CD3 BsAb and MUC-1/CD3 BsAb elicited antitumor immunity by T-cell adoptive immunotherapy in lung cancer
In this study, we combined two BsAbs to achieve a dual-target therapy strategy of EpCAM+ and MUC-1+ with high affinity and specificity. The results showed that the combination of two BsAbs against EpCAM and MUC-1 could inhibit the growth of lung cancer more effectively in cell lines and primary tumors. The superior antitumor effect of two BsAbs could be attributable to enhanced CTL and increased production of type I IFNs. At the same time, the combination of EpCAM/CD3 BsAb and MUC-1/CD3 BsAb significantly regulated T population in the TDLNs. Therefore, we have found a potential immunotherapeutic strategy, which was the com...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Ce Wang Shang Chen Yingjuan Wu Di Wu Jingbo Wang Furong Li Source Type: research

Hypothesis: Immunotherapy by Selective Convalescent Blood Engineering to Stifle Diseases like COVID-19
This report incorporates all these aspects by suggesting a novel treatment therapy called selective convalescent leukapheresis and transfusion (SCLT) and also highlights its potential in vaccination. The anticipated advantages of the proposed technique outweigh the cost, time, and efficiency of other available transfusion and vaccination processes. It is envisioned that in the future this new approach could serve as a rapid emergency response to subdue a pathogen outbreak and to stop it from becoming an epidemic, or pandemic.PMID:34522165 | PMC:PMC8436102 | DOI:10.7150/ijms.46363 (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Arka Prava Mukherjee Source Type: research

Chest CT abnormalities in COVID-19: a systematic review
Int J Med Sci. 2021 Aug 1;18(15):3395-3402. doi: 10.7150/ijms.50568. eCollection 2021.ABSTRACTComputed tomography (CT) of the chest is one of the main diagnositic tools for coronavirus disease 2019 (COVID-19) infection. To document the chest CT findings in patients with confirmed COVID-19 and their association with the clinical severity, we searched related literatures through PubMed, MEDLINE, Embase, Web of Science (inception to May 4, 2020) and reviewed reference lists of previous systematic reviews. A total of 31 case reports (3768 patients) on CT findings of COVID-19 were included. The most common comorbid conditions w...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Ramy Abou Ghayda Keum Hwa Lee Jae Seok Kim Seul Lee Sung Hwi Hong Kyeong Seok Kim Kyeong Eon Kim Jinhyn Seok Hajeong Kim Jangsuk Seo Seungmin Lee Ai Koyanagi Louis Jacob Lee Smith Han Li Andreas Kronbichler Jae Il Shin Source Type: research

Cobalt chloride-stimulated hypoxia promotes the proliferation of cholesteatoma keratinocytes via the PI3K/Akt signaling pathway
Int J Med Sci. 2021 Aug 2;18(15):3403-3411. doi: 10.7150/ijms.60617. eCollection 2021.ABSTRACTHerein, we purposed to explore whether hypoxia triggers proliferation of cholesteatoma keratinocytes via the PI3K-Akt signaling cascade. Cells were inoculated with different concentration of CoCl2. The proliferation and cellular HIF-1α, p-PDK1 and p‑Akt expression levels of cholesteatoma keratinocytes were assessed in vitro. Hypoxia escalated cell proliferation via upregulating p-PDK1 and p‑Akt expressions. Specific inhibitor of the PI3K-Akt signaling cascade, LY294002 markedly inhibited the expression of p‑Akt and sig...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Chen Zhang Min Chen Qi Tao Zhangcai Chi Source Type: research

Integrated analysis of the molecular mechanisms in idiopathic pulmonary fibrosis
Conclusion: These findings provide a new research direction for the diagnosis and treatment of IPF.PMID:34522168 | PMC:PMC8436110 | DOI:10.7150/ijms.61309 (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Ke Zhu Aiqun Xu Wanli Xia Pulin Li Rui Han Enze Wang Sijing Zhou Ran Wang Source Type: research

Identification of Key Genes and Pathways associated with Endometriosis by Weighted Gene Co-expression Network Analysis
Conclusion: Our study identified, for the first time, the hub genes and enrichment pathways in the EC and EU using WGCNA, which may provide a comprehensive understanding of the pathogenesis of endometriosis and have important clinical implications for the treatment and diagnosis of endometriosis.PMID:34522169 | PMC:PMC8436105 | DOI:10.7150/ijms.63541 (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Jingni Wu Xiaoling Fang Xiaomeng Xia Source Type: research

Simultaneous silencing Aurora-A and UHRF1 inhibits colorectal cancer cell growth through regulating expression of DNMT1 and STAT1
Int J Med Sci. 2021 Aug 5;18(15):3437-3451. doi: 10.7150/ijms.61969. eCollection 2021.ABSTRACTAurora-A has attracted a great deal of interest as a potential therapeutic target for patients with CRC. However, the outcomes of inhibitors targeting Aurora-A are not as favorable as expected, and the basis behind the ineffectiveness remains unknown. Here, we found that signal transducer and activator of transcription 1 (STAT1) was highly expressed in colorectal cancer (CRC) xenograft mouse models that were resistant to alisertib, an Aurora-A inhibitor. Unexpectedly, we found that alisertib disrupted Aurora-A binding with ubiquit...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Jing Han Xin Chen Jiawei Xu Laili Chu Rongqing Li Na Sun Zhen Jiang Hongyang Liu Xing Ge Junnian Zheng Jing Yang Takayuki Ikezoe Source Type: research

Areca nut extract (ANE) inhibits the progression of hepatocellular carcinoma cells via activation of ROS production and activation of autophagy
In conclusion, our study demonstrated that the ANE may be a new potential compound for HCC therapy.PMID:34522171 | PMC:PMC8436086 | DOI:10.7150/ijms.61570 (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Po-Li Wei Chin-Sheng Hung Hsuan-Hsuan Lu Uyanga Batzorig Chien-Yu Huang Yu-Jia Chang Source Type: research

Verification of the Efficacy of New Diagnostic Criteria for Retropharyngeal Nodes in a Cohort of Nasopharyngeal Carcinoma Patients
Int J Med Sci. 2021 Aug 13;18(15):3463-3469. doi: 10.7150/ijms.58375. eCollection 2021.ABSTRACTPurpose: A multistage approach to diagnose lateral retropharyngeal nodes (LRPNs) of nasopharyngeal carcinoma (NPC) had been proposed and warranted for validation. Methods: Between 2012 and 2017, the patients with newly diagnosed NPC were enrolled. The responsive nodes or those that progressed during follow-up were positive. The criteria for the multistage approach delimited LRPNs with a minimal axial diameter (MIAD) ≥ 6.1 mm were assessed as positive and if the mean standard uptake value ≥ 2.6, or if the maximal coronal dia...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Dom-Gene Tu Hsuan-Yu Chen Wei-Jen Yao Yu-Sheng Hung Yu-Kang Chang Chih-Han Chang Yu-Wen Wang Source Type: research

Sex Difference in the Associations among Obesity-Related Indices with Metabolic Syndrome in Patients with Type 2 Diabetes Mellitus
Int J Med Sci. 2021 Aug 13;18(15):3470-3477. doi: 10.7150/ijms.63180. eCollection 2021.ABSTRACTBackground: The aim of this study was to investigate the associations among obesity-related indices and MetS in diabetic patients, and explore sex differences in these associations. Methods: Patients with type 2 DM were included from two hospitals in southern Taiwan. The Adult Treatment Panel III criteria for an Asian population were used to define MetS. In addition, the following obesity-related indices were evaluated: waist-to-height ratio, waist-hip ratio (WHR), conicity index (CI), body mass index (BMI), body roundness index,...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Hsiu-Fen Jao Chih-Hsuan Wung Hui-Chen Yu Mei-Yueh Lee Po-Chih Chen Szu-Chia Chen Jer-Ming Chang Source Type: research

Bioinformatics analysis identified shared differentially expressed genes as potential biomarkers for Hashimoto's thyroiditis-related papillary thyroid cancer
Conclusions: We identified that expression of LTF and CCL21 are significantly different in HT and PTC, suggesting an underlying association between HT and PTC.PMID:34522174 | PMC:PMC8436097 | DOI:10.7150/ijms.63402 (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Chang Liu Yu Pan Qinyu Li Yifan Zhang Source Type: research

Comparison of short- and long-axis nerve hydrodissection for carpal tunnel syndrome: A prospective randomized, single-blind trial
This study is to compare the efficacy of short-axis hydrodissection with long-axis hydrodissection for patients with mild-to-moderate carpal tunnel syndrome (CTS). Methods: Forty-seven patients with mild-to-moderate CTS were enrolled in a prospective, randomized, single-blinded, controlled trial (6 months follow-up). With ultrasound guidance, patients in both groups (short-axis or long-axis groups) were injected with normal saline (5 mL per session). Assessments were performed before and 2 weeks after the injection, as well as at 1, 3, and 6 months post-intervention. The primary outcome measure was the Boston Carpal Tunnel...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Si-Ru Chen Tsung-Yen Ho Yu-Ping Shen Tsung-Ying Li Yu-Chi Su King Hei Stanley Lam Liang-Cheng Chen Yung-Tsan Wu Source Type: research

Expression and localization of Sox10 during hair follicle morphogenesis and induced hair cycle
Int J Med Sci. 2021 Aug 13;18(15):3498-3505. doi: 10.7150/ijms.60728. eCollection 2021.ABSTRACTSox transcription factors play many diverse roles during development, including regulating stem cell states, directing differentiation, and influencing the local chromatin landscape. Sox10 has been implicated in the control of stem/progenitor activity and epithelial-mesenchymal transition, yet it has not been studied in relation to the hair follicle cycle or hair follicle stem cell (HFSC) control. To elucidate the role of Sox10 in hair follicle cycle control, we performed immunohistochemical and immunofluorescence analysis of its...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Jing Jing Peng Xu Jia-Li Xu Yu-Xin Ding Xiao-Shuang Yang Xiao-Qin Jin Li-Juan Zhou Yu-Hong Chen Xian-Jie Wu Zhong-Fa Lu Source Type: research

Downregulation of circ_0000673 Promotes Cell Proliferation and Migration in Endometriosis via the Mir-616-3p/PTEN Axis
Int J Med Sci. 2021 Aug 17;18(15):3506-3515. doi: 10.7150/ijms.63564. eCollection 2021.ABSTRACTEndometriosis is a common gynecological disease, affecting up to 10% of women of reproductive age and approximately 50% of women with infertility. Circular RNAs (circRNAs) have been shown to be involved in a number of diseases. Dysregulated expression of circRNAs in endometriosis has been reported, and circ_0000673 was significantly downregulated. However, the details of its role in the pathogenesis of endometriosis are still poorly understood. We investigated the location and effects of the downregulation of circ_0000673 in endo...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Yongwen Yang Deying Ban Chun Zhang Licong Shen Source Type: research

PLK1 Inhibition Induces Immunogenic Cell Death and Enhances Immunity against NSCLC
In this study, we found that inhibiting PLK1 could alter the tumor immune microenvironment by increasing DC maturation, and enriching T cells infiltration. PLK1 inhibitors, serving as immunogenic cell death (ICD) inducers, indirectly activated DCs, instead of directly acting on DC cells, through the surface expression of costimulatory molecules on and enhanced phagocytosis by DCs. Furthermore, upon targeting PLK1, tumor cells that had undergone ICD were converted into an endogenous vaccine, which triggered the immune memory responses and protected the mice from tumor challenge. Collectively, these results suggested that th...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Jie Zhou Qifan Yang Lisen Lu Zhan Tuo Zhexing Shou Jing Cheng Source Type: research

Risk factor comparison in young patients presenting with acute coronary syndrome with atherosclerotic coronary artery disease vs. angiographically normal coronaries
Int J Med Sci. 2021 Aug 21;18(15):3526-3532. doi: 10.7150/ijms.60869. eCollection 2021.ABSTRACTBackground: Acute myocardial infarction is a relatively rare phenomenon in the young population. The incidence has nevertheless increased from years past, likely due to the presence of multiple risk factors from an increasingly younger age. Regardless of whether they have atherosclerotic coronary artery disease or normal coronary angiogram, young patients with risk factors for coronary artery disease (CAD), chest pain, and positive troponin, are initially treated in a similar fashion. Our goal was to shed light on whether risk fa...
Source: International Journal of Medical Sciences - September 15, 2021 Category: Biomedical Science Authors: Sarah Jamil Gohar Jamil Hanaa Mesameh Anwer Qureshi Juma AlKaabi Charu Sharma Faisal Aziz Ali Rashed Al-Shamsi Javed Yasin Source Type: research