Evaluation of a novel monoclonal antibody against tumor-associated MUC1 for diagnosis and prognosis of breast cancer.
Authors: Stergiou N, Nagel J, Pektor S, Heimes AS, Jäkel J, Brenner W, Schmidt M, Miederer M, Kunz H, Roesch F, Schmitt E Abstract There is still a great unmet medical need concerning diagnosis and treatment of breast cancer which could be addressed by utilizing specific molecular targets. Tumor-associated MUC1 is expressed on over 90 % of all breast cancer entities and differs strongly from its physiological form on epithelial cells, therefore presenting a unique target for breast cancer diagnosis and antibody-mediated immune therapy. Utilizing an anti-tumor vaccine based on a synthetically prepared glycopept...
Source: International Journal of Medical Sciences - October 9, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

The impact of PRDX4 and the EGFR mutation status on cellular proliferation in lung adenocarcinoma.
Conclusions: The PRDX4 expression and EGFR mutation status were significantly associated with the prognosis of patients with stage I LUAD, and EGFR mutations affected the role of PRDX4 in the proliferation of LUAD cells. PMID: 31588184 [PubMed - in process] (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - October 9, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Parvalbumin Promoter Methylation Altered in Major Depressive Disorder.
Conclusion: This study provides the first evidence of abnormalities of PVALB promoter methylation in MDD and its correlation with MDD severity indicating a role for epigenetics in this psychiatric disorder. PMID: 31588185 [PubMed - in process] (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - October 9, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Living Donor Liver Transplantation in Biliary Atresia Children with Pulmonary Hypertension.
CONCLUSION: LDLT is a safe procedure in a selected group of BA patients with PH, however, further long-term clinical investigations and mechanical researches are needed. PMID: 31588186 [PubMed - in process] (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - October 9, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Circulating and Pulmonary T-cell Populations Driving the Immune Response in Non-HIV Immunocompromised Patients with Pneumocystis jirovecii Pneumonia.
Conclusions: Our findings suggest that pulmonary expansion of Th1 and Tc9 subsets may play protective and detrimental roles in PCP patients, respectively. Thus, these specific T-cell subsets in the lungs may serve as targeted immunotherapies for patients with PCP. PMID: 31588187 [PubMed - in process] (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - October 9, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Loss of Circulating Exosomal miR-92b is a Novel Biomarker of Colorectal Cancer at Early Stage.
Authors: Min L, Chen L, Liu S, Yu Y, Guo Q, Li P, Zhu S Abstract Early diagnosis of colorectal cancer (CRC) is clinically critical but technically challenging, especially in a minimal-invasive way. Emerging evidence suggests that exosome-encapsulated microRNAs (miRNAs) is a kind of promising cancer biomarker. Here we investigated the predictive potential of exosomal miR-92b in plasma samples obtained from 114 participants [40 CRC, 22 colorectal adenomas (CA), 52 non-neoplasm controls (NC)] by RT-qPCR. We found that exosomal miR-92b level was significantly down-regulated in CRC patients compared with CA and NC patie...
Source: International Journal of Medical Sciences - October 9, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Exosomes Released by Bone Marrow Mesenchymal Stem Cells Attenuate Lung Injury Induced by Intestinal Ischemia Reperfusion via the TLR4/NF- κB Pathway.
Conclusions: MSC-derived exosomes provide protection similar to that of MSCs against IIR-induced ALI via inhibition of TLR4/NF-κB signaling, suggesting that a potential strategy against IIR-mediated acute lung injury could be therapy with exosomes as a non-cellular alternative to MSC transplantation. PMID: 31588189 [PubMed - in process] (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - October 9, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Increased resting-state functional connectivity in suprasellar tumor patients with postoperative visual improvement.
In this study, we aimed to investigate alterations in brain functional connectivity (FC) in suprasellar tumor patients with visual improvement using resting-state functional magnetic resonance imaging (rs-fMRI). Methods: This longitudinal study was conducted on 13 suprasellar tumor patients who had ophthalmological examinations and rs-fMRI at the following time points: within 1-week preoperation (Pre-op), 1-week postoperation (Post-1w) and 1-month postoperation (Post-1m). The visual impairment score (VIS), local functional correlation (LCOR) and FC values were subjected to one-way ANOVA. Pearson correlation coefficients be...
Source: International Journal of Medical Sciences - October 9, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Protein chip and bioinformatic analyses of differentially expressed proteins involved in the effect of hydrogen-rich water on myocardial ischemia-reperfusion injury.
Authors: Li L, Liu T, Li X, Liu X, Liu L, Li S, Li Z, Zhou Y, Liu F Abstract Background: The differentially expressed proteins (DEPs) involved in the effect of hydrogen-rich water on myocardial ischemia reperfusion injury (MIRI) and their biological processes and signaling pathway were analyzed. Methods: 20 Wistar rats were randomly and equally divided into a control and a hydrogen-rich group. Hearts were removed and fixed in a Langendorff device. The control group was perfused with K-R solution, and the hydrogen-rich water group was perfused with K-R solution + hydrogen-rich water. Protein was extracted from the v...
Source: International Journal of Medical Sciences - October 9, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Fluoxetine ameliorates dysbiosis in a depression model induced by chronic unpredicted mild stress in mice.
Conclusions: This study revealed that fluoxetine led to restoration of dysbiosis induced by stress stimulation, which may imply a possible pathway through which one CNS target drug plays its role in reshaping the gut microbiota. PMID: 31588192 [PubMed - in process] (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - October 9, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Dual-function of Baicalin in nsPEFs-treated Hepatocytes and Hepatocellular Carcinoma cells for Different Death Pathway and Mitochondrial Response.
Authors: Wang Y, Yin S, Zhou Y, Zhou W, Chen T, Wu Q, Zhou L, Zheng S Abstract Nanosecond pulsed electric fields (nsPEFs) is emerged as a potential curative modality to ablate hepatocellular carcinoma (HCC). The application of local ablation is usually limited by insufficiency of liver function. While baicalin, a flavonoid isolated from Scutellaria baicalensis Georgi, has been proven to possess both anti-tumor and protective effects. Our study aimed to estimate different responses of hepatic cancer cells and hepatocytes to the combination of nsPEFs and baicalin. Cell viability, apoptosis and necrosis, mitochondrial...
Source: International Journal of Medical Sciences - October 9, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Effects of semi-solidification of enteral nutrients on the pharmacokinetic behavior of orally administered carbamazepine in rats.
Authors: Nagai K, Omotani S, Shibano M, Kobayashi A, Ito A, Nishimura I, Hatsuda Y, Mukai J, Teramachi H, Myotoku M Abstract The use of semi-solid enteral nutrients plays an extremely important role in accurate nutrition management. In the present study, we compared the pharmacokinetic profile of orally administered carbamazepine (CBZ) in rats treated with liquid RACOL®, semi-solid RACOL®, and HINE E-gel®, which are enteral nutrients marketed in Japan. Since liquid and semi-solid formulations are both marketed in Japan for RACOL®, liquid RACOL® was orally administered to control rats. The serum ...
Source: International Journal of Medical Sciences - October 9, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Impact of general anaesthesia on endoplasmic reticulum stress: propofol vs. isoflurane.
Conclusion: Significant higher of ER stress from blood and liver were observed in rats under anaesthesia with isoflurane, compared to those that received propofol. ROS from blood also showed significant higher under anaesthesia with isoflurane. However, these findings were not associated with any changes in cytokines in blood or immunohistochemical assay in tissues. PMID: 31588195 [PubMed - in process] (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - October 9, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Adverse reaction profiles of hemorrhagic adverse reactions caused by direct oral anticoagulants analyzed using the Food and Drug Administration Adverse Event Reporting System (FAERS) database and the Japanese Adverse Drug Event Report (JADER) database.
Authors: Shimada K, Hasegawa S, Nakao S, Mukai R, Sasaoka S, Ueda N, Kato Y, Abe J, Mori T, Yoshimura T, Kinosada Y, Nakamura M Abstract Direct oral anticoagulants (DOACs) are used in anticoagulant therapy. The purpose of this study was to evaluate the association of DOAC-induced gastrointestinal (GI) and nervous system hemorrhage using the FDA's Adverse Event Reporting System (FAERS) database and the Japanese Adverse Drug Event Report (JADER) database. We identified and analyzed the reports of hemorrhagic reactions between 2004 and 2016 from the FAERS and JADER databases, and calculated the adjusted reported odds ...
Source: International Journal of Medical Sciences - October 9, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Mild hypothermia during the reperfusion phase protects mitochondrial bioenergetics against ischemia-reperfusion injury in an animal model of ex-vivo liver transplantation-an experimental study.
Authors: Martins RM, Teodoro JS, Furtado E, Oliveira RC, Tralhão JG, Rolo AP, Palmeira CM Abstract The organ preservation paradigm has changed following the development of new ways to preserve organs. The use of machine perfusion to preserve organs appears to have several advantages compared with conventional static cold storage. For liver transplants, the temperature control provided by machine perfusion improves organ preservation. In this experimental study, we measured the effects of different temperatures on mitochondrial bioenergetics during the reperfusion phase. An experimental model of ex-vivo liver...
Source: International Journal of Medical Sciences - October 9, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Preimplantation Genetic Testing for Monogenic Disease of Spinal Muscular Atrophy by Multiple Displacement Amplification: 11 unaffected livebirths.
Conclusion: Careful handling during the MDA procedure can improve subsequent PCR efficiency and reduce the ADO rate. We suggest that this protocol is reliable for increasing the accuracy of the PGT-M for SMA. PMID: 31588198 [PubMed - in process] (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - October 9, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Nephrolithiasis predicts ischemic stroke: A longitudinal follow-up study using a national sample cohort.
This study sought to evaluate associations between nephrolithiasis and hemorrhagic and ischemic stroke using a national sample cohort from Korea. Data from 2002 to 2013 were collected for individuals ≥ 20 years of age in the Korean National Health Insurance Service (NHIS)-National Sample Cohort. We extracted nephrolithiasis patients (n = 22,636) and 1:4 matched controls (n = 90,544) and analyzed the occurrence of stroke. Matching was performed based on age, sex, income, region of residence, hypertension, diabetes mellitus, and dyslipidemia history. Crude and adjusted hazard ratios (HRs) were calculated using Cox proport...
Source: International Journal of Medical Sciences - September 17, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Identification of Novel Genes in Osteoarthritic Fibroblast-Like Synoviocytes Using Next-Generation Sequencing and Bioinformatics Approaches.
Authors: Chen YJ, Chang WA, Wu LY, Huang CF, Chen CH, Kuo PL Abstract Synovitis in osteoarthritis (OA) the consequence of low grade inflammatory process caused by cartilage breakdown products that stimulated the production of pro-inflammatory mediators by fibroblast-like synoviocytes (FLS). FLS participate in joint homeostasis and low grade inflammation in the joint microenvironment triggers FLS transformation. In the current study, we aimed to identify differentially expressed genes and potential miRNA regulations in human OA FLS through deep sequencing and bioinformatics approaches. The 245 differentially express...
Source: International Journal of Medical Sciences - September 17, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Cellular Substrates for Cell-Based Tissue Engineering of Human Corneal Endothelial Cells.
Authors: Zhu Q, Sun H, Yang D, Tighe S, Liu Y, Zhu Y, Hu M Abstract Corneal endothelial tissue engineering aims to find solutions for blindness due to endothelial dysfunction. A suitable combination of endothelial cells, substrates and environmental cues should be deployed for engineering functional endothelial tissues. This manuscript reviews up-to-date topics of corneal endothelial tissue engineering with special emphasis on biomaterial substrates and their properties, efficacy, and mechanisms of supporting functional endothelial cells in vitro. PMID: 31523168 [PubMed - in process] (Source: International Jour...
Source: International Journal of Medical Sciences - September 17, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Yunnan Baiyao reduces hospital-acquired pressure ulcers via suppressing virulence gene expression and biofilm formation of Staphylococcus aureus.
Authors: Liu J, Cai M, Yan H, Fu J, Wu G, Zhao Z, Zhao Y, Wang Y, Sun Y, You Y, Lin L, Huang J, Huang R, Zeng J Abstract Yunnan Baiyao (YB) as a kind of famous Chinese herbal medicine, possessed hemostatic, invigorating the circulation of blood, and anti-inflammatory effects. Identifying strategies to protect patients at risk for hospital-acquired pressure ulcers (HAPU) is essential. Herein, our results showed that YB treatment can effectively reduce the acne wound area and improve efficacy in a comparative study of 60 cases HAPU patients with S. aureus positive of acne wound pathogens. Furthermore, YB inhibited HI...
Source: International Journal of Medical Sciences - September 17, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C polymorphisms in breast cancer: a Sardinian preliminary case-control study.
Authors: Castiglia P, Sanna V, Azara A, De Miglio MR, Murgia L, Pira G, Sanges F, Fancellu A, Carru C, Bisail M, Muroni MR Abstract Two common polymorphisms in the MTHFR gene, C677T and A1298C, are associated with reduced enzyme activity and may be associated with breast cancer susceptibility. We performed a case-control study to investigate the association between the two SNPs in the MTHFR gene and risk of breast cancer. In total, 58 breast cancer patients and 58 unaffected controls were enrolled in the study. Polymerase chain reaction/restriction fragment length polymorphism technique (PCR-RFLP) was conducted to ...
Source: International Journal of Medical Sciences - September 17, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Utility of human papillomavirus L1 capsid protein and HPV test as prognostic markers for cervical intraepithelial neoplasia 2+ in women with persistent ASCUS /LSIL cervical cytology.
Authors: Ki EY, Park JS, Lee A, Kim TJ, Jin HT, Seo YB, Gen Y, Park MY, Lee SJ Abstract Objective: Efficient and highly predictive biomarkers reflecting the prognosis of persistent atypical squamous cells of unknown significance(ASCUS) and low grade squamous intraepithelial lesion(LSIL)s are unavailable and need to be developed urgently. We aimed to develop a predictive model for diagnosis of cervical intraepithelial neoplasia(CIN)2+ by analyzing the immunocytochemical expression of the HPV L1 capsid protein in patients with persistent ASCUS and LSIL with a high risk of HPV infection. Methods: Cervical cytology sam...
Source: International Journal of Medical Sciences - September 17, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

A new method to confirm the absence of human and animal serum in mesenchymal stem cell culture media.
In this study, we attempted to establish further the potential of this gene as a new marker indicative of the presence of serum in media. Using a real-time quantitative PCR gene array screening containing 84 functional genes, we verified BDKRB1 as a specific gene upregulated in the presence of serum. The expression of BDKRB1 in Ad-MSCs was induced not only by bovine serum but also by human serum. The BDKRB1 expression was induced even when Ad-MSCs was cultured with 0.1% serum in the medium. We concluded that BDKRB1 is a valuable marker to detect traces of both human and animal serum in Ad-MSCs cultures. Our study provides ...
Source: International Journal of Medical Sciences - September 17, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

The extracts of Astragalus membranaceus enhance chemosensitivity and reduce tumor indoleamine 2, 3-dioxygenase expression.
Authors: Phacharapiyangkul N, Wu LH, Lee WY, Kuo YH, Wu YJ, Liou HP, Tsai YE, Lee CH Abstract Astragalus membranaceus has been shown to possess anti-inflammation and antitumor properties. Several studies have indicated that extracts of Astragalus membranaceus (PG2) have growth inhibitory effects on tumor. However, the effect of PG2 on enhancing the chemotherapy, modulating tumor immune escape and their mechanism of action is unknown and need further investigation. Connexin (Cx) 43 is ubiquitous in cells and involved in facilitating the passage of chemotherapeutic drugs to bystander tumor cells. The indoleamine 2, 3...
Source: International Journal of Medical Sciences - September 17, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Poncirus Trifoliata (L.) Raf. Extract Inhibits the Development of Atopic Dermatitis-like Lesions in Human Keratinocytes and NC/Nga mice.
This study investigated the anti-allergic effect of Poncirus trifoliata (L.) Raf. (PT) on human keratinocytic HaCaT cells in vitro and on 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis-like lesions in vivo. The release of TARC, MCP-1, IL-6 and IL-8 is increased by IFN-γ and TNF-α in HaCaT cells, and PT extract suppressed the increased production of TARC, MCP-1, IL-6, and IL-8. PT extract recovered the expression of filaggrin decreased by IFN-γ and TNF-α. in vivo experiment, PT administration decreased the skin severity score, thickening of the epidermis, movement of inflammatory cells int...
Source: International Journal of Medical Sciences - September 17, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

High mobility group box 1 protein (HMGB1) as biomarker in hypoxia-induced persistent pulmonary hypertension of the newborn: a clinical and in vivo pilot study.
Conclusions: This study indicates that HMGB1 is related to hypoxia-induced PPHN pathogenesis. HMGB1 changes might thus be used as an early indicator to diagnose hypoxia-induced PPHN and evaluate its improvement. We also provide important evidence for the involvement of inflammation in the progression of hypoxia-induced PPHN. PMID: 31523175 [PubMed - in process] (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 17, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Ribosomal Protein L15 is involved in Colon Carcinogenesis.
In this study, we examine the role of RPL15, a large ribosomal subunit protein, in human colon carcinogenesis. Our results reveal that RPL15 is remarkably upregulated in human primary colon cancer tissues and cultured cell lines when compared with paired non-cancerous tissues and non-transformed epithelium cells. Elevated expression of RPL15 in colon cancer tissues is closely correlated with clinicopathological characteristics in patients. We determine the effects of RPL15 on nucleolar maintenance, ribosomal biogenesis and cell proliferation in human cells. We show that RPL15 is required for maintenance of nucleolar struct...
Source: International Journal of Medical Sciences - September 17, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Expression pattern of CDK12 protein in gastric cancer and its positive correlation with CD8+ cell density and CCL12 expression.
Authors: Ji J, Zhou C, Wu J, Cai Q, Shi M, Zhang H, Yu Y, Zhu Z, Zhang J Abstract Background: The aims of this study were to investigate the expression pattern of CDK12 protein in gastric cancer, and to analyze the correlations of CDK12 expression between CD8+ cell density and CCL12 expression. Methods: Eighty-six paired tumor and non-tumor samples were collected from patients who underwent radical surgery and had pathological confirmed gastric adenocarcinoma. Immunohistochemistry was used to assess CDK12 expression and CD8+ cell density. Expression of CDK12 and CCL21 mRNA was detected by quantitative reverse trans...
Source: International Journal of Medical Sciences - September 17, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

The phenotype of vascular smooth muscle cells co-cultured with endothelial cells is modulated by PDGFR- β/IQGAP1 signaling in LPS-induced intravascular injury.
Conclusion The phenotype of vascular SMCs co-cultured with ECs was modulated by IQGAP1 through the PDGFR-β pathway, which may lead to vascular remodeling and homeostasis in LPS-induced intravascular injury. This pathway could be a novel target for the treatment of vascular damage. PMID: 31523178 [PubMed - in process] (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 17, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

MRC-5 Cancer-associated Fibroblasts Influence Production of Cancer Stem Cell Markers and Inflammation-associated Cell Surface Molecules, in Liver Cancer Cell Lines.
Conclusion: MRC-5 extensively affected the production of CSC markers and inflammation-associated cell surface molecules. Tumor-targeting molecular therapies should consider these findings. PMID: 31523179 [PubMed - in process] (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 17, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Elevated plasma S100A1 level is a risk factor for ST-segment elevation myocardial infarction and associated with post-infarction cardiac function.
We examined the plasma S100A1 level in 207 STEMI patients (STEMI group) and 217 clinically healthy subjects for routine physical examination without a history of coronary artery disease (Control group). Baseline characteristics and concentrations of relevant biomarkers were compared. The relationship between S100A1 and other plasma biomarkers was detected using correlation analysis. The predictive role of S100A1 on occurrence of STEMI was then assessed using multivariate ordinal regression model analysis after adjusting for other covariates. RESULTS: The plasma S100A1 level was found to be significantly higher (P
Source: International Journal of Medical Sciences - September 17, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Greater Protective Potent of s-Methyl Cysteine and Syringic Acid Combination for NGF-differentiated PC12 Cells against Kainic acid-induced Injury.
Conclusion: These findings suggested that this combination, SMC+SA, might provide greater protective potent for neuronal cells. PMID: 31523181 [PubMed - in process] (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - September 17, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Impact of matrix metalloproteinase-11 gene polymorphisms on development and clinicopathologcial variables of uterine cervical cancer in Taiwanese women.
In conclusion, it indicates no impact of the MMP-11 SNPs on uterine cervical cancer in Taiwanese women. PMID: 31337950 [PubMed - in process] (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - July 26, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Increased Expression of LAMTOR5 Predicts Poor Prognosis and Is Associated with Lymph Node Metastasis of Head and Neck Squamous Cell Carcinoma.
Authors: Yang QC, Wu CC, Cao LY, Xiao Y, Li H, Liu B, Sun ZJ Abstract Late endosomal/lysosomal adaptor and MAPK and mTOR activator 5 (LAMTOR5) is a novel oncoprotein associated with several human malignancies, but its clinical role in head and neck squamous cell carcinoma (HNSCC) remains unclear. The present study aims to investigate the clinical and pathological significance of LAMTOR5 in HNSCC. We utilized immunohistochemical staining of human tissue microarrays (210 primary HNSCC, 42 normal oral mucosae, 69 oral epithelial dysplasia, and 68 metastasis lymph nodes) to explore the clinical and pathological signifi...
Source: International Journal of Medical Sciences - July 26, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

A Prediction Model of Essential Hypertension Based on Genetic and Environmental Risk Factors in Northern Han Chinese.
Conclusions: The present study identified environmental and genetic risk factors for EH in northern Han Chinese population and constructed prediction models for SBP and DBP. PMID: 31337952 [PubMed - in process] (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - July 26, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Identification of Key Genes and Pathways in Cervical Cancer by Bioinformatics Analysis.
Authors: Wu X, Peng L, Zhang Y, Chen S, Lei Q, Li G, Zhang C Abstract Cervical cancer is a common malignant tumour of the female reproductive system that seriously threatens the health of women. The aims of this study were to identify key genes and pathways and to illuminate new molecular mechanisms underlying cervical cancer. Altogether, 1829 DEGs were identified, including 794 significantly down-regulated DEGs and 1035 significantly up-regulated DEGs. GO analysis suggested that the up-regulated DEGs were mainly enriched in mitotic cell cycle processes, including DNA replication, organelle fission, chromosome segr...
Source: International Journal of Medical Sciences - July 26, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Altered expression of renin-angiotensin system receptors throughout colorectal adenoma-adenocarcinoma sequence.
Conclusions: These results suggest that: 1) RAS receptors are differentially regulated as the genetic and epigenetic alterations accumulate throughout the uninvolved mucosa-adenoma-CRC sequence. 2) Loss of AT2 expression could contribute to the aggressive behavior of advanced CRC cells. PMID: 31337954 [PubMed - in process] (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - July 26, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Low-Frequency Electrical Stimulation of Denervated Skeletal Muscle Retards Muscle and Trabecular Bone Loss in Aged Rats.
Authors: Tamaki H, Yotani K, Ogita F, Hayao K, Kirimto H, Onishi H, Kasuga N, Yamamoto N Abstract Electrical stimulation (ES)-induced muscle contraction has multiple effects; however, mechano-responsiveness of bone tissue declines with age. Here, we investigated whether daily low-frequency ES-induced muscle contraction treatment reduces muscle and bone loss and ameliorates bone fragility in early-stage disuse musculoskeletal atrophy in aged rats. Twenty-seven-month-old male rats were assigned to age-matched groups comprising the control (CON), sciatic nerve denervation (DN), or DN with direct low-frequency ES (DN+E...
Source: International Journal of Medical Sciences - July 26, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Sensory Nerves Affect Bone Regeneration in Rabbit Mandibular Distraction Osteogenesis.
Authors: Cao J, Zhang S, Gupta A, Du Z, Lei D, Wang L, Wang X Abstract Objectives: To investigate the effects of inferior alveolar nerve on new bone formation in rabbit mandibular distraction osteogenesis. Methods: 20 New Zealand White rabbits underwent bilateral distraction osteogenesis with a rate of 1 mm/day. The inferior alveolar nerve of one side was resected under the surgical microscope, with the inferior alveolar vascular intact. The contralateral side received sham operation. The rabbits were sacrificed at consolidation time of 28 days. The regenerate callus underwent radiograph examination, dual-energy X-...
Source: International Journal of Medical Sciences - July 26, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Nocardiosis in Kidney Disease Patients under Immunosuppressive Therapy: Case Report and Literature Review.
Authors: Wang T, Jia Y, Chu B, Liu H, Dong X, Zhang Y Abstract The increased use of novel and powerful immunosuppressive drugs in kidney diseases may concomitantly expose the patients to higher risk of opportunistic infections, some of which still remain underdiagnosed thus mishandled. As such, we recently had a less prepared encounter of pulmonary nocardial infection in an ANCA-associated renal vasculitis patient under steroid therapy. Despite the use of broad-spectrum antimicrobials including micafungin, the infection was still unbridled and eventually culminated in lethal brain abscess. We thus chose to renew th...
Source: International Journal of Medical Sciences - July 26, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

A Combination of Tamarindus indica seeds and Curcuma longa Rhizome Extracts Improves Knee Joint Function and Alleviates Pain in Non-Arthritic Adults Following Physical Activity.
Authors: Rao PS, Ramanjaneyulu YS, Prisk VR, Schurgers LJ Abstract Background: Knee joint pain is the most common reason for physical disability which associates with age. TamaFlexTM (NXT15906F6) is a synergistic anti-inflammatory formulation which contains ethanol/aqueous extracts of Tamarindus indica seeds and ethanol extract of Curcuma longa rhizome. Methods: In a 90-day randomized, double-blind, placebo-controlled study, we evaluated efficacy of NXT15906F6 in relieving pain and improving joint function in non-arthritic adults. Ninety non-arthritic subjects who experienced knee pain and joint discomfort followin...
Source: International Journal of Medical Sciences - July 26, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Hepatocyte growth factor plays a particular role in progression of overall cardiac damage in experimental pulmonary hypertension.
Conclusion: We suggest that cardiac overexpression of Hgf might contribute to increased plasma HGF in MCT-induced PH. HGF could be exploited as a cardiospecific biomarker and HGF/MET pathway as a target in drug discovery for PH. PMID: 31337959 [PubMed - in process] (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - July 26, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

TIMD4 rs6882076 SNP Is Associated with Decreased Levels of Triglycerides and the Risk of Coronary Heart Disease and Ischemic Stroke.
Authors: Khounphinith E, Yin RX, Cao XL, Huang F, Wu JZ, Li H Abstract Background: The T-cell immunoglobulin and mucin domain 4 gene (TIMD4) rs6882076 single nucleotide polymorphism (SNP) has been associated with serum total cholesterol, low-density lipoprotein cholesterol and triglycerides (TG) levels, but the results are inconsistent. Moreover, little is known about such association in Chinese populations. The aim of this study was to detect the association of the TIMD4 rs6882076 SNP and serum lipid levels and the risk of coronary heart disease (CHD) and ischemic stroke (IS) in a Southern Chinese Han population. ...
Source: International Journal of Medical Sciences - July 26, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Differential Analysis of Hypertension-Associated Intestinal Microbiota.
In this study, fecal samples from 62 cases with normal blood pressure (BP) and 67 cases with high BP were used for 16S amplicon sequencing. Sixty-one cases of HTN and 61 corresponding cases with normal BP were obtained by propensity score matching (PSM), and differential analysis was conducted using the DEseq2 package. PSM was also used to match six IDH patients with six controls and to match 35 cases of SH with 35 controls. There were 54 differential genera between the HTN and normal BP groups, and there were five differential genera between the IDH and normal BP groups. There were 38 differential genera between the SH an...
Source: International Journal of Medical Sciences - July 26, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Microarray and proteome array in an atherosclerosis mouse model for identification of biomarkers in whole blood.
In this study, we screened potential biomarker genes and proteins from whole blood of apolipoprotein E knockout (ApoE-/- ) mice maintained on a Western diet, by comparing them to ApoE+/+ mice. We used whole blood for microarray and proteome array. Candidate genes and proteins identified from each method were confirmed with quantitative real-time PCR and ELISA. Based on our data, we speculate that Lilrb4a, n-R5s136, and IL-5 are potential targets that can be developed into novel biomarkers of atherosclerosis. Our study contributes to the diagnosis of atherosclerosis using whole blood in clinical settings. PMID: 31337962...
Source: International Journal of Medical Sciences - July 26, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Differential Response of Non-cancerous and Malignant Breast Cancer Cells to Conditioned Medium of Adipose tissue-derived Stromal Cells (ASCs).
Conclusions: Overall, this study demonstrated that cancerous and non-cancerous breast cells respond differently to ASC-derived CM. ASC-derived CM triggered significant cell death in breast cancer cell lines, however non-cancerous breast cells exhibited dissimilar response to ASC-derived CM. PMID: 31337963 [PubMed - in process] (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - July 26, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

NONRATT021972 long-noncoding RNA: A promising lncRNA in diabetes-related diseases.
Authors: Suwal A, Hao JL, Liu XF, Zhou DD, Pant OP, Gao Y, Hui P, Dai XX, Lu CW Abstract Diabetes mellitus (DM) is a principal health problem with increasing incidence worldwide. It can be associated with various systemic diseases. Long non-coding RNA (lncRNA), a member of non-coding RNA has been newly linked with various human diseases. Recent evidence from animal experiments has shown that the incidence and development of type 2 diabetes are contributed by the atypical expression of lncRNA in which the biomarker with capable clinical potential was lncRNA NONRATT021972. In this review, we demonstrated the numerous...
Source: International Journal of Medical Sciences - July 26, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Possible association of arrestin domain-containing protein 3 and progression of non-alcoholic fatty liver disease.
Authors: Ogawa M, Kanda T, Higuchi T, Takahashi H, Kaneko T, Matsumoto N, Nirei K, Yamagami H, Matsuoka S, Kuroda K, Moriyama M Abstract The prevalence of non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH) is increasing worldwide. Several effective drugs for these diseases are now in development and under clinical trials. It is important to reveal the mechanism of the development of NAFLD and NASH. We investigated the role of arrestin domain-containing protein 3 (ARRDC3), which is linked to obesity in men and regulates body mass, adiposity and energy expenditure, in the progression o...
Source: International Journal of Medical Sciences - July 26, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

The molecular landscape of histone lysine methyltransferases and demethylases in non-small cell lung cancer.
Conclusions: Our result suggests that alteration of histone methylation is strongly involved in NSCLC. Some histone methylation related genes might serve as potential prognosis predictor or therapeutic target for NSCLC. The significance of some histone methylation related genes was contrary to the literature and awaits further validation. PMID: 31341405 [PubMed - in process] (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - July 26, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

First-line Screening of OXPHOS Deficiencies Using Microscale Oxygraphy in Human Skin Fibroblasts: A Preliminary Study.
Authors: Germain N, Dessein AF, Vienne JC, Dobbelaere D, Mention K, Joncquel M, Dekiouk S, Laine W, Kluza J, Marchetti P Abstract The diagnosis of mitochondrial diseases is a real challenge because of the vast clinical and genetic heterogeneity. Classically, the clinical examination and genetic analysis must be completed by several biochemical assays to confirm the diagnosis of mitochondrial disease. Here, we tested the validity of microscale XF technology in measuring oxygen consumption in human skin fibroblasts isolated from 5 pediatric patients with heterogeneous mitochondrial disorders. We first set up the prot...
Source: International Journal of Medical Sciences - July 26, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research