Deletion of the lipid droplet protein kinase gene affects lipid droplets biogenesis, parasite infectivity, and resistance to trivalent antimony in < i > Leishmania infantum < /i >
by Juliana Martins Ribeiro, Paula Alves Silva, H éllida Marina Costa-Silva, Ana Maria Murta Santi, Silvane Maria Fonseca Murta
The Lipid Droplet Protein Kinase (LDK) facilitates lipid droplet (LD) biogenesis, organelles involved in various metabolic and signaling functions in trypanosomatids. As LDK ’s function has not been previously explored inLeishmania spp., we utilized CRISPR/Cas9 technology to generateLDK-knockout lines ofLeishmania infantum to investigate its role in this parasite. Our findings demonstrate thatLDK is not an essential gene forL.infantum, as its deletion did not impede parasite survival. Furthermo...
Source: PLoS Neglected Tropical Diseases - January 18, 2024 Category: Tropical Medicine Authors: Juliana Martins Ribeiro Source Type: research
Pediatric cutaneous mucormicosis
A 9-year-old boy diagnosed with acute myeloblastic leukemia and undergoing chemotherapy, was admitted with febrile neutropenia. During his admission, several violaceous plaques appeared on the upper extremities and anterior left hemithorax, which worsened and acquired a necrotic center. We performed a biopsy and histology showed a cutaneous infarction at the dermoepidermal and subcutaneous level. We observed abundant wide hyphae with right-angled branching and a culture isolated Rhizopus oryzae. A plastic surgery consultant performed a surgical debridement of the lesions and treatment was started with intravenous amphoteri...
Source: Dermatology Online Journal - January 18, 2024 Category: Dermatology Authors: Matei, Mariana Claudia Source Type: research
In vitro characterization and molecular epidemiology of Cryptococcus spp. isolates from non-HIV patients in Guangdong, China
ConclusionPhenotypically variant and non-wild-type strains were found in Guangdong, and a significant proportion of these strains had MIC values near the ECV values towards the 6 antifungal drugs, and resistance was observed for 4 out of 6 drugs. The molecular type was highly homogeneous but compositionally diverse, with rare types found. Enhanced surveillance of the aetiology and evolution and continuous monitoring of antifungal susceptibility are needed to provide references for decision-making in the health sector and optimization of disease prevention and control. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - January 15, 2024 Category: Microbiology Source Type: research
Computer-made peptide RQ18 acts as a dual antifungal and antibiofilm peptide though membrane-associated mechanisms of action
Arch Biochem Biophys. 2024 Jan 11;753:109884. doi: 10.1016/j.abb.2024.109884. Online ahead of print.ABSTRACTThe spread of fungi resistant to conventional drugs has become a threatening problem. In this context, antimicrobial peptides (AMPs) have been considered as one of the main alternatives for controlling fungal infections. Here, we report the antifungal and antibiofilm activity and some clues about peptide RQ18's mechanism of action against Candida and Cryptococcus. This peptide inhibited yeast growth from 2.5 μM and killed all Candida tropicalis cells within 2 h incubation. Moreover, it showed a synergistic effect wi...
Source: Archives of Biochemistry and Biophysics - January 13, 2024 Category: Biochemistry Authors: Claudiane Vilharroel Almeida Caio Fernando Ramalho de Oliveira Lu ís Henrique de Oliveira Almeida Suellen Rodrigues Ramalho Camila de Oliveira Gutierrez Janaina de Cassia Orlandi Sardi Oct ávio Luiz Franco Marlon Henrique Cardoso Maria Ligia Rodrigues M Source Type: research
Gastrointestinal bleeding in a kidney transplant recipient
We report a case of disseminated histoplasmosis in a kidney transplant recipient whose initial presentation was acute lower gastrointestinal bleeding with no other symptoms. The colonoscopy showed scattered punchout circular colonic ulcers with biopsy revealing budding yeasts consistent with a diagnosis of histoplasmosis. The patient was successfully treated with a prolonged course of intravenous amphotericin B followed by oral itraconazole.PMID:38214175 | DOI:10.5414/CN111298 (Source: Clinical Nephrology)
Source: Clinical Nephrology - January 12, 2024 Category: Urology & Nephrology Authors: Navneet Kaur Michael Vutam Guillermo Orlando Padilla Neeraj Singh Source Type: research
Fungicide-tolerant persister formation during cryptococcal pulmonary infection
Cell Host Microbe. 2024 Feb 14;32(2):276-289.e7. doi: 10.1016/j.chom.2023.12.012. Epub 2024 Jan 11.ABSTRACTBacterial persisters, a subpopulation of genetically susceptible cells that are normally dormant and tolerant to bactericides, have been studied extensively because of their clinical importance. In comparison, much less is known about the determinants underlying fungicide-tolerant fungal persister formation in vivo. Here, we report that during mouse lung infection, Cryptococcus neoformans forms persisters that are highly tolerant to amphotericin B (AmB), the standard of care for treating cryptococcosis. By exploring s...
Source: Cell Host and Microbe - January 12, 2024 Category: Microbiology Authors: Weixin Ke Yuyan Xie Yingying Chen Hao Ding Leixin Ye Haoning Qiu Hao Li Lanyue Zhang Lei Chen Xiuyun Tian Zhenghao Shen Zili Song Xin Fan Jian-Fa Zong Zhengyan Guo Xiaoyu Ma Meng Xiao Guojian Liao Cui Hua Liu Wen-Bing Yin Zhiyang Dong Feng Yang Yuan-Ying Source Type: research
< em > In vitro < /em > activity of manogepix and comparators against infrequently encountered yeast and mold isolates from the SENTRY Surveillance Program (2017-2022)
This study determined the in vitro activity of manogepix and comparators against a large collection of infrequently encountered yeast and molds. Manogepix demonstrated potent in vitro activity against infrequently encountered yeasts exhibiting elevated MIC values to other drug classes, including Candida spp. (MIC50/90, 0.008/0.12 mg/L), Saprochaete clavata (Magnusiomyces clavatus) (MIC50/90, 0.03/0.06 mg/L), Magnusiomyces capitatus (MICrange, 0.016-0.06 mg/L), Rhodotorula minuta (MIC, 0.016 mg/L), and Rhodotorula mucilaginosa (MIC50/90, 0.03/0.12 mg/L). Similarly, manogepix was active against infrequently encountered mold ...
Source: Antimicrobial Agents and Chemotherapy - January 11, 2024 Category: Microbiology Authors: Michael Pfaller Michael Huband Paul A Bien Cecilia G Carvalhaes Abby Klauer Mariana Castanheira Source Type: research
Antifungal activity of the repurposed drug disulfiram against Cryptococcus neoformans
This study investigated the antifungal effects and the mechanism of action of DSF against C. neoformans to provide a new theoretical foundation for the treatment of Cryptococcal infections. In vitro studies demonstrated that DSF inhibited Cryptococcus growth at minimum inhibitory concentrations (MICs) ranging from 1.0 to 8.0 μg/mL. Combined antifungal effects have been observed for DSF with 5-fluorocytosine, amphotericin B, terbinafine, or ketoconazole. DSF exerts significant protective effects and synergistic effects combined with 5-FU for Galleria mellonella infected with C. neoformans. Mechanistic investigations showe...
Source: Frontiers in Pharmacology - January 11, 2024 Category: Drugs & Pharmacology Source Type: research
MALDI-TOF mass spectrometry identification and antifungal susceptibility testing of yeasts causing vulvovaginal candidiasis (VVC) in Tebessa (Northeastern Algeria)
Ann Biol Clin (Paris). 2024 Jan 1;81(6). doi: 10.1684/abc.2023.1852. Online ahead of print.ABSTRACTVulvovaginal candidiasis (VVC) alongside with antifungal resistance are becoming a major clinical problem in recent years. A prospective study aimed to evaluate the diversity of yeast strains associated with VVC in Tebessa city (northeastern Algeria) and investigate their susceptibility patterns. Over two months, yeasts were isolated on chromogenic medium from twenty-nine non-pregnant women with symptomatic VVC. The isolates were characterized with MALDI-TOF MS and antifungal susceptibility testing was performed for nine anti...
Source: Annales de Biologie Clinique - January 8, 2024 Category: Biochemistry Authors: Mabrouka Benhadj Taha Menasria St éphane Ranque Source Type: research
MALDI-TOF mass spectrometry identification and antifungal susceptibility testing of yeasts causing vulvovaginal candidiasis (VVC) in Tebessa (Northeastern Algeria)
Ann Biol Clin (Paris). 2024 Jan 1;81(6). doi: 10.1684/abc.2023.1852. Online ahead of print.ABSTRACTVulvovaginal candidiasis (VVC) alongside with antifungal resistance are becoming a major clinical problem in recent years. A prospective study aimed to evaluate the diversity of yeast strains associated with VVC in Tebessa city (northeastern Algeria) and investigate their susceptibility patterns. Over two months, yeasts were isolated on chromogenic medium from twenty-nine non-pregnant women with symptomatic VVC. The isolates were characterized with MALDI-TOF MS and antifungal susceptibility testing was performed for nine anti...
Source: Annales de Biologie Clinique - January 8, 2024 Category: Biochemistry Authors: Mabrouka Benhadj Taha Menasria St éphane Ranque Source Type: research
MALDI-TOF mass spectrometry identification and antifungal susceptibility testing of yeasts causing vulvovaginal candidiasis (VVC) in Tebessa (Northeastern Algeria)
Ann Biol Clin (Paris). 2024 Jan 1;81(6). doi: 10.1684/abc.2023.1852. Online ahead of print.ABSTRACTVulvovaginal candidiasis (VVC) alongside with antifungal resistance are becoming a major clinical problem in recent years. A prospective study aimed to evaluate the diversity of yeast strains associated with VVC in Tebessa city (northeastern Algeria) and investigate their susceptibility patterns. Over two months, yeasts were isolated on chromogenic medium from twenty-nine non-pregnant women with symptomatic VVC. The isolates were characterized with MALDI-TOF MS and antifungal susceptibility testing was performed for nine anti...
Source: Annales de Biologie Clinique - January 8, 2024 Category: Biochemistry Authors: Mabrouka Benhadj Taha Menasria St éphane Ranque Source Type: research
MALDI-TOF mass spectrometry identification and antifungal susceptibility testing of yeasts causing vulvovaginal candidiasis (VVC) in Tebessa (Northeastern Algeria)
Ann Biol Clin (Paris). 2024 Jan 1;81(6). doi: 10.1684/abc.2023.1852. Online ahead of print.ABSTRACTVulvovaginal candidiasis (VVC) alongside with antifungal resistance are becoming a major clinical problem in recent years. A prospective study aimed to evaluate the diversity of yeast strains associated with VVC in Tebessa city (northeastern Algeria) and investigate their susceptibility patterns. Over two months, yeasts were isolated on chromogenic medium from twenty-nine non-pregnant women with symptomatic VVC. The isolates were characterized with MALDI-TOF MS and antifungal susceptibility testing was performed for nine anti...
Source: Annales de Biologie Clinique - January 8, 2024 Category: Biochemistry Authors: Mabrouka Benhadj Taha Menasria St éphane Ranque Source Type: research
MALDI-TOF mass spectrometry identification and antifungal susceptibility testing of yeasts causing vulvovaginal candidiasis (VVC) in Tebessa (Northeastern Algeria)
Ann Biol Clin (Paris). 2024 Jan 1;81(6). doi: 10.1684/abc.2023.1852. Online ahead of print.ABSTRACTVulvovaginal candidiasis (VVC) alongside with antifungal resistance are becoming a major clinical problem in recent years. A prospective study aimed to evaluate the diversity of yeast strains associated with VVC in Tebessa city (northeastern Algeria) and investigate their susceptibility patterns. Over two months, yeasts were isolated on chromogenic medium from twenty-nine non-pregnant women with symptomatic VVC. The isolates were characterized with MALDI-TOF MS and antifungal susceptibility testing was performed for nine anti...
Source: Annales de Biologie Clinique - January 8, 2024 Category: Biochemistry Authors: Mabrouka Benhadj Taha Menasria St éphane Ranque Source Type: research
MALDI-TOF mass spectrometry identification and antifungal susceptibility testing of yeasts causing vulvovaginal candidiasis (VVC) in Tebessa (Northeastern Algeria)
Ann Biol Clin (Paris). 2024 Jan 1;81(6). doi: 10.1684/abc.2023.1852. Online ahead of print.ABSTRACTVulvovaginal candidiasis (VVC) alongside with antifungal resistance are becoming a major clinical problem in recent years. A prospective study aimed to evaluate the diversity of yeast strains associated with VVC in Tebessa city (northeastern Algeria) and investigate their susceptibility patterns. Over two months, yeasts were isolated on chromogenic medium from twenty-nine non-pregnant women with symptomatic VVC. The isolates were characterized with MALDI-TOF MS and antifungal susceptibility testing was performed for nine anti...
Source: Annales de Biologie Clinique - January 8, 2024 Category: Biochemistry Authors: Mabrouka Benhadj Taha Menasria St éphane Ranque Source Type: research
MALDI-TOF mass spectrometry identification and antifungal susceptibility testing of yeasts causing vulvovaginal candidiasis (VVC) in Tebessa (Northeastern Algeria)
Ann Biol Clin (Paris). 2024 Jan 1;81(6). doi: 10.1684/abc.2023.1852. Online ahead of print.ABSTRACTVulvovaginal candidiasis (VVC) alongside with antifungal resistance are becoming a major clinical problem in recent years. A prospective study aimed to evaluate the diversity of yeast strains associated with VVC in Tebessa city (northeastern Algeria) and investigate their susceptibility patterns. Over two months, yeasts were isolated on chromogenic medium from twenty-nine non-pregnant women with symptomatic VVC. The isolates were characterized with MALDI-TOF MS and antifungal susceptibility testing was performed for nine anti...
Source: Annales de Biologie Clinique - January 8, 2024 Category: Biochemistry Authors: Mabrouka Benhadj Taha Menasria St éphane Ranque Source Type: research
