Myricanol improves metabolic profiles in dexamethasone induced lipid and protein metabolism disorders in mice
This study for the first time applied widely targeted metabolomics to investigate the beneficial effects of MY on glucose, lipids, and protein metabolism in DEX-induced metabolic abnormality in mice. The results showed that MY significantly reversed DEX-induced soleus and gastrocnemius muscle weight loss, muscle fiber damage, and muscle strength loss. MY alleviated DEX-induced metabolic disorders by increasing SIRT1 and glucose transporter type 4 (GLUT4) expressions. Additionally, myricanol prevented muscle cell apoptosis and atrophy by inhibiting caspase 3 cleavages and muscle ring-finger protein-1 (MuRF1) expression. Met...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - April 7, 2024 Category: Drugs & Pharmacology Authors: Tiandan Li Xiaochao Hu Lingyang Fan Yong Yang Kai He Source Type: research
Myricanol improves metabolic profiles in dexamethasone induced lipid and protein metabolism disorders in mice
This study for the first time applied widely targeted metabolomics to investigate the beneficial effects of MY on glucose, lipids, and protein metabolism in DEX-induced metabolic abnormality in mice. The results showed that MY significantly reversed DEX-induced soleus and gastrocnemius muscle weight loss, muscle fiber damage, and muscle strength loss. MY alleviated DEX-induced metabolic disorders by increasing SIRT1 and glucose transporter type 4 (GLUT4) expressions. Additionally, myricanol prevented muscle cell apoptosis and atrophy by inhibiting caspase 3 cleavages and muscle ring-finger protein-1 (MuRF1) expression. Met...
Source: Atherosclerosis - April 7, 2024 Category: Cardiology Authors: Tiandan Li Xiaochao Hu Lingyang Fan Yong Yang Kai He Source Type: research
Myricanol improves metabolic profiles in dexamethasone induced lipid and protein metabolism disorders in mice
This study for the first time applied widely targeted metabolomics to investigate the beneficial effects of MY on glucose, lipids, and protein metabolism in DEX-induced metabolic abnormality in mice. The results showed that MY significantly reversed DEX-induced soleus and gastrocnemius muscle weight loss, muscle fiber damage, and muscle strength loss. MY alleviated DEX-induced metabolic disorders by increasing SIRT1 and glucose transporter type 4 (GLUT4) expressions. Additionally, myricanol prevented muscle cell apoptosis and atrophy by inhibiting caspase 3 cleavages and muscle ring-finger protein-1 (MuRF1) expression. Met...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - April 7, 2024 Category: Drugs & Pharmacology Authors: Tiandan Li Xiaochao Hu Lingyang Fan Yong Yang Kai He Source Type: research
Myricanol improves metabolic profiles in dexamethasone induced lipid and protein metabolism disorders in mice
This study for the first time applied widely targeted metabolomics to investigate the beneficial effects of MY on glucose, lipids, and protein metabolism in DEX-induced metabolic abnormality in mice. The results showed that MY significantly reversed DEX-induced soleus and gastrocnemius muscle weight loss, muscle fiber damage, and muscle strength loss. MY alleviated DEX-induced metabolic disorders by increasing SIRT1 and glucose transporter type 4 (GLUT4) expressions. Additionally, myricanol prevented muscle cell apoptosis and atrophy by inhibiting caspase 3 cleavages and muscle ring-finger protein-1 (MuRF1) expression. Met...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - April 7, 2024 Category: Drugs & Pharmacology Authors: Tiandan Li Xiaochao Hu Lingyang Fan Yong Yang Kai He Source Type: research
pH-dependent regulation of an acidophilic < em > O < /em > -acetylhomoserine sulfhydrylase from < em > Lactobacillus plantarum < /em >
Appl Environ Microbiol. 2024 Apr 3:e0011824. doi: 10.1128/aem.00118-24. Online ahead of print.ABSTRACTBacteria have two routes for the l-methionine biosynthesis. In one route called the direct sulfuration pathway, acetylated l-homoserine is directly converted into l-homocysteine. The reaction using H2S as the second substrate is catalyzed by a pyridoxal 5'-phosphate-dependent enzyme, O-acetylhomoserine sulfhydrylase (OAHS). In the present study, we determined the enzymatic functions and the structures of OAHS from Lactobacillus plantarum (LpOAHS). The LpOAHS enzyme exhibited the highest catalytic activity under the weak ac...
Source: Applied and Environmental Microbiology - April 3, 2024 Category: Microbiology Authors: Yasuyuki Matoba Kosuke Oda Maho Wataeda Hina Kanemori Koichi Matsuo Source Type: research
Boosting the immunogenicity of the CoronaVac SARS-CoV-2 inactivated vaccine with Huoxiang Suling Shuanghua Decoction: a randomized, double-blind, placebo-controlled study
ConclusionOral HSSD boosts the immunogenicity of the CVS vaccine in young and adult individuals. This trial provides clinical reference for evaluation of TCM immunomodulators to improve the immune response to COVID-19 vaccines. (Source: Frontiers in Immunology)
Source: Frontiers in Immunology - April 3, 2024 Category: Allergy & Immunology Source Type: research
Biochemical and structural impact of two novel missense mutations in cystathionine beta-synthase gene associated with homocystinuria
In this study, we present the clinical and biochemical characterization of two novel CBS missense mutations that do not respond to pyridoxine treatment, namely c.689T>A (L230Q) and 215A>T (K72I), identified in a Chinese patient. We observed that the disease-associated K72I genetic variant had no apparent effects on the spectroscopic and catalytic properties of the full-length enzyme. In contrast, the L230Q variant expressed in E. coli did not fully retain heme and when compared to the wild-type enzyme, it exhibited more significant impairments in both the canonical cystathionine-synthesis and the alternative H2S-prod...
Source: The Biochemical Journal - April 2, 2024 Category: Biochemistry Authors: Duaa Al-Sadeq Carolina Conter Angelos Thanassoulas Nader Al-Dewik Bared Safieh-Garabedian Luis Alfonso Luis Alfonso Mart ínez-Cruz Gheyath Nasrallah Alessandra Astegno Michail Nomikos Source Type: research
Biochemical and structural impact of two novel missense mutations in cystathionine beta-synthase gene associated with homocystinuria
In this study, we present the clinical and biochemical characterization of two novel CBS missense mutations that do not respond to pyridoxine treatment, namely c.689T>A (L230Q) and 215A>T (K72I), identified in a Chinese patient. We observed that the disease-associated K72I genetic variant had no apparent effects on the spectroscopic and catalytic properties of the full-length enzyme. In contrast, the L230Q variant expressed in E. coli did not fully retain heme and when compared to the wild-type enzyme, it exhibited more significant impairments in both the canonical cystathionine-synthesis and the alternative H2S-prod...
Source: The Biochemical Journal - April 2, 2024 Category: Biochemistry Authors: Duaa Al-Sadeq Carolina Conter Angelos Thanassoulas Nader Al-Dewik Bared Safieh-Garabedian Luis Alfonso Luis Alfonso Mart ínez-Cruz Gheyath Nasrallah Alessandra Astegno Michail Nomikos Source Type: research
Multivitamins co-intake can reduce the prevalence of kidney stones: a large-scale cross-sectional study
ConclusionsOur research revealed that high levels of vitamin B6 and vitamin D intake were linked to a lower prevalence of kidney stone. With the gradual increase intake of vitamin C, the prevalence of kidney calculi decreased first and then increased. In addition, the co-exposure of nine vitamins is a protective factor for kidney stone disease. (Source: International Urology and Nephrology)
Source: International Urology and Nephrology - April 2, 2024 Category: Urology & Nephrology Source Type: research
l ‐Theanine Prevents Colonic Damage via NF‐κB/MAPK Signaling Pathways Induced by a High‐Fat Diet in Rats
Conclusionsl-Thea alleviates colitis generated by an HFD by restoring the integrity of the intestinal barrier, suppressing inflammation through regulation of MAPK/NF- κB signaling pathways, and enhancing microbial metabolism in colon. (Source: Molecular Nutrition and Food Research)
Source: Molecular Nutrition and Food Research - March 29, 2024 Category: Food Science Authors: Chunyan Fang,
Yifeng Shen,
Ziyang Ma,
Yuchen Li,
Jingyi Zhang,
Chen Liu,
Yulong Ye Tags: Research Article Source Type: research
Causal Relationships Between Gut Microbiota, Inflammatory Cells/Proteins, and Subarachnoid Hemorrhage: A Multi-omics Bidirectional Mendelian Randomization Study and Meta-analysis
This study combined various omics approaches with Mendelian randomization analysis, including MR-IVW, MR Egger, MR weight median, and MR weight mode, to generate preliminary results. It also employed reverse Mendelian randomization, treating SAH as the exposure. Finally, a meta -analysis was conducted to summarize these findings. The study found positive correlations between SAH and both GBPA-Pyridoxal 5 phosphate biosynthesis I (OR=1.48, 95% CI, 1.04–2.12) and GBPA-glucose biosynthesis I (OR=0.68, 95% CI, 0.52–0.90). Increased levels of urokinase-type plasma activator were also associated with SAH (OR=1.17, 95% CI, 1....
Source: Molecular Neurobiology - March 25, 2024 Category: Neurology Source Type: research
Biosynthesis of Strained Amino Acids by a PLP-Dependent Enzyme through Cryptic Halogenation
Angew Chem Int Ed Engl. 2024 Mar 22:e202319344. doi: 10.1002/anie.202319344. Online ahead of print.ABSTRACTAmino acids (AAs) are modular building blocks which nature uses to synthesize both macromolecules, such as proteins, and small molecule natural products, such as alkaloids and non-ribosomal peptides. While the 20 main proteinogenic AAs display relatively limited side chain diversity, a wide range of non-canonical amino acids (ncAAs) exist that are not used by the ribosome for protein synthesis, but contain a broad array of structural features and functional groups. In this communication, we report the discovery of the...
Source: Angewandte Chemie - March 22, 2024 Category: Chemistry Authors: Max B Sosa Jacob T Leeman Lorenzo J Washington Henrik V Scheller Michelle Chang Source Type: research
A review on L-methioninase in cancer therapy: Precision targeting, advancements and diverse applications for a promising future
Int J Biol Macromol. 2024 Mar 18:130997. doi: 10.1016/j.ijbiomac.2024.130997. Online ahead of print.ABSTRACTCancer remains a global health challenge, demanding novel therapeutic options due to the debilitating side effects of conventional treatments on healthy tissues. The review highlights the potential of L-methioninase, a pyridoxal-5-phosphate (PLP)-dependent enzyme, as a promising avenue in alternative cancer therapy. L-methioninase offers a unique advantage, its ability to selectively target and inhibit the growth of cancer cells without harming healthy cells. This selectivity arises because tumor cells lack an essent...
Source: International Journal of Biological Macromolecules - March 20, 2024 Category: Biochemistry Authors: Bhumi M Javia Megha S Gadhvi Suhas J Vyas Anjana Ghelani Nengah Wirajana Dushyant R Dudhagara Source Type: research
A review on L-methioninase in cancer therapy: Precision targeting, advancements and diverse applications for a promising future
Int J Biol Macromol. 2024 Mar 18:130997. doi: 10.1016/j.ijbiomac.2024.130997. Online ahead of print.ABSTRACTCancer remains a global health challenge, demanding novel therapeutic options due to the debilitating side effects of conventional treatments on healthy tissues. The review highlights the potential of L-methioninase, a pyridoxal-5-phosphate (PLP)-dependent enzyme, as a promising avenue in alternative cancer therapy. L-methioninase offers a unique advantage, its ability to selectively target and inhibit the growth of cancer cells without harming healthy cells. This selectivity arises because tumor cells lack an essent...
Source: International Journal of Biological Macromolecules - March 20, 2024 Category: Biochemistry Authors: Bhumi M Javia Megha S Gadhvi Suhas J Vyas Anjana Ghelani Nengah Wirajana Dushyant R Dudhagara Source Type: research
Metabolic and physiological adaptations of microalgal growth-promoting bacterium Azospirillum brasilense growing under biogas atmosphere: a microarray-based transcriptome analysis
Arch Microbiol. 2024 Mar 16;206(4):173. doi: 10.1007/s00203-024-03890-z.ABSTRACTUsing microalgal growth-promoting bacteria (MGPB) to improve the cultured microalga metabolism during biotechnological processes is one of the most promising strategies to enhance their benefits. Nonetheless, the culture condition effect used during the biotechnological process on MGPB growth and metabolism is key to ensure the expected positive bacterium growth and metabolism of microalgae. In this sense, the present research study investigated the effect of the synthetic biogas atmosphere (75% CH4-25% CO2) on metabolic and physiological adapt...
Source: Archives of Microbiology - March 16, 2024 Category: Microbiology Authors: Carolina Garciglia-Mercado Claudia A Contreras Francisco J Choix Luz E de-Bashan Gracia A G ómez-Anduro Oskar A Palacios Source Type: research
